三种介类中药生、煅品水煎液中无机元素含量分析研究
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摘要
[目的]测定牡蛎、石决明和瓦楞子三种介类中药生、煅品水煎液中无机元素的含量,初步探讨该类中药中无机元素含量与药物性味、功效的关系。[方法]采用微波消解法对三种介类中药生、煅水煎液样品进行处理后,使用电感耦合等离子体原子发射光谱(inductively coupled plasma optical emission spectrometry,ICP-OES)法分别测定三种介类中药生、煅品水煎液中Ca、Mg、Fe、K、Cu、Zn、Mn、Co、Mo、Se、Cr共11种无机元素的含量,并比较其含量差异。[结果]三种介类中药生品水煎液中Ca、Mg、K三种无机元素含量较高,其他无机元素含量相对较低,各无机元素含量高低顺序趋势大致相同:Ca>Mg>K>Mn>Fe>Cu或Zn>Se(Cr)>Mo(Co)。牡蛎、石决明生品水煎液中Mg、Mn、Fe元素含量均高于瓦楞子生品水煎液(P<0.05)。三种介类中药煅品水煎液中Ca元素含量均较各自生品水煎液增高(P<0.05),K、Se、Cr元素含量与各自生品水煎液比较无统计学差异(P>0.05)。与生品水煎液比较,三种介类中药煅品水煎液中Mg、Mn、Fe、Cu、Zn、Mo、Co元素含量变化差异较大。[结论]牡蛎、石决明和瓦楞子三种介类中药的性味、功效差异可能与无机元素种类及含量差异有关。
[Objective] To determine the contents of mineral elements in the decoction of three testacean traditional Chinese medicines(Oysters, Abalone shell,Concha arcae) and processed one, by comparing the differences of their contents, the relationship between mineral elements and nature and efficacy of testacean TCM were discussed. [Methods] The samples were digested by microwave digestion system, and the amounts of 11 mineral elements(Ca, Mg, Fe, K,Cu, Zn, Mn, Co, Mo, Se, Cr) were determined by inductively coupled plasma optical emission spectrometry(ICP-OES), and the differences of their contents were compared. [Results] The order of the content of each element in the decoction of three testacean TCM had the same trend: Ca >Mg>K>Mn>Fe>Cu or Zn>Se(Cr)>Mo(Co). The contents of Ca, Mg and K were higher in the raw water decoction of three testacean TCM, the contents of other mineral elements were relatively low. The contents of Mg,Mn and Fe in the raw decoction of Oysters, Abalone shell were higher than those of the raw decoction of Concha arcae( P<0.05). The content of Ca in the three calcined decoction was higher than that of the raw decoction( P<0.05), the contents of K, Se and Cr were not significantly different from those of raw water decoction(P>0.05), the contents changes of calcinated decoction on Mg, Mn, Fe, Cu, Zn, Co, Mo elements with nothing in common.[Conclusion] The difference in the performance of the three testacean TCM and calcined products may be related to the mineral elements.
[Objective] To determine the contents of mineral elements in the decoction of three testacean traditional Chinese medicines(Oysters, Abalone shell,Concha arcae) and processed one, by comparing the differences of their contents, the relationship between mineral elements and nature and efficacy of testacean TCM were discussed. [Methods] The samples were digested by microwave digestion system, and the amounts of 11 mineral elements(Ca, Mg, Fe, K,Cu, Zn, Mn, Co, Mo, Se, Cr) were determined by inductively coupled plasma optical emission spectrometry(ICP-OES), and the differences of their contents were compared. [Results] The order of the content of each element in the decoction of three testacean TCM had the same trend: Ca >Mg>K>Mn>Fe>Cu or Zn>Se(Cr)>Mo(Co). The contents of Ca, Mg and K were higher in the raw water decoction of three testacean TCM, the contents of other mineral elements were relatively low. The contents of Mg,Mn and Fe in the raw decoction of Oysters, Abalone shell were higher than those of the raw decoction of Concha arcae( P<0.05). The content of Ca in the three calcined decoction was higher than that of the raw decoction( P<0.05), the contents of K, Se and Cr were not significantly different from those of raw water decoction(P>0.05), the contents changes of calcinated decoction on Mg, Mn, Fe, Cu, Zn, Co, Mo elements with nothing in common.[Conclusion] The difference in the performance of the three testacean TCM and calcined products may be related to the mineral elements.
引文
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[12]盛良.论化学元素具有寒热温凉四性--论中药四性与现代化学的统一[J].中国中医基础医学杂志,2003,9(1):14-15,29.SHENG Liang.Theory of chemical elements and four properties of traditional Chinese medicine--on the unity of traditional Chinese medicine and modern chemistry[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2003,9(1):14-15,29.
[13]谭晓梅,王新雨,张明明,等.5种贝壳类动物药及其煎出物中微量元素含量测定[J].中国实验方剂学杂志,2011,17(1):61-63.TAN Xiaomei,WANG Xinyu,ZHANG Mingming,et al.Determination of microelement of testacean traditional Chinese medicine[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,17(1):61-63.
[14]李珊珊,孙立卿,周长会,等.治疗高血压病中草药中微量元素的主成分分析[J].南阳理工学院学报,2014,6(6):114-116.LI Shanshan,SUN Liqin,ZHOU Changhui,et al.Principal component analysis of micro-elements in the treatment of hypertension on Chinese herbal medicine[J].Journal of Nanyang Institute of Technology,2014,6(6):114-116.
[15]杨柳,张义生,徐惠芳,等.矿物类中药石膏清热作用研究进展[J].中国药师,2016,19(10):1943-1945.YANG Liu,ZHANG Yisheng,XU Huifang,et al.Research progress in heat-clearing activity of mineral drug Gypsum[J].China Pharmacist,2016,19(10):1943-1945.
[16]罗琴,张锦元,谢启明,等.彝药方杆蕨中微量元素含量与药性关系的研究[J].广东微量元素科学,2013,20(6):23-27.LUO Qin,ZHANG Jinyuan,XIE Qiming,et al.Study of the relationship between trace element content and property of medicine in Yi nationality herbal medicine glaphylopteridopsis erubescens[J].Guangdong Trace Element Science,2013,20(6):23-27.
[17]江胜丽.中药炮制对微量元素的总量和溶出性影响[J].世界最新医学信息文摘,2015,15(41):163.JIANG Shengli.Effect of Chinese medicine processing on total amount and dissolution of trace elements[J].World Latest Medicine Information,2015,15(41):163.
[18]姜威,李晶峰,高久堂,等.石决明的化学成分及药理作用[J].吉林中医药,2015,35(3):272-274.JIANG Wei,LI Jingfeng,GAO Jiutang,et al.Chemical constituents and pharmacological effects of Abalone Shell[J].Jilin Journal of Traditional Chinese Medicine,2015,35(3):272-274.
[2]钟赣生.中药学[M].3版.北京:中国中医药出版社,2014:319,342,344.ZHONG Gansheng.Chinese Pharmacy[M].3rd edition.Beijing:China Press of Traditional Chinese Medicine,2014:319,342,344.
[3]彭倩倩,洪寅,廖广辉.6种介类中药对高血压肝阳上亢大鼠“平肝潜阳”作用的实验研究[J].中国中医急症,2014,23(6):1016-1018.PENG Qianqian,HONG Yin,LIAO Guanghui.Experimental study of six testacean TCM on the mechanism of"Calming the Liver and Suppressing Yang"in rats with hypertension of hyperactivity of liver yang[J].Chinese Journal of Emergency in Traditional Chinese Medicine,2014,23(6):1016-1018.
[4]彭倩倩,洪寅,廖广辉.6种介类中药对大鼠甲状腺肿大模型影响的实验研究[J].浙江中医药大学学报,2013,37(12):29-32.PENG Qianqian,HONG Yin,LIAO Guanghui.Experimental study of six testacean TCM"Endometriosis"role on goiter model rats[J].Journal of Zhejiang Chinese Medical University,2013,37(12):29-32.
[5]司玮,阿如娜,李尚蓉,等.7种海洋矿物药的比较分析研究[J].中国中药杂志,2014,39(17):3321-3325.SI Wei,A Ru'na,LI Shangrong,et al.Comparative analysis of seven marine biological source of mineral drugs[J].Chinese Journal of Traditional Chinese Medicine,2014,39(17):3321-3325.
[6]韦正,陈鸿平,陈林,等.石决明、牡蛎、珍珠母三味平肝潜阳药碳酸钙的含量测定及其比较研究[J].中药与临床,2012,3(4):10-13.WEI Zheng,CHEN Hongping,CHEN Lin,et al.Comparative study on the contents of calcium carbonate in three calming liver and suppressing liver-yang herbs Haliotidis concha,Ostreae concha and Margaritifera concha[J].Pharmacy and Clinics of Chinese Materia Medica,2012,3(4):10-13.
[7]李雪莲,杨丽,赵梓辰,等.碳酸钙类矿物药红外光谱比较研究[J].时珍国医国药,2014,25(10):2418-2419.LI Xuelian,YANG Li,ZHAO Zichen,et al.Study on comparative of calcium carbonate mineral medicines by infrared spectrum[J].Lishizhen Medicine and Materia Medica Research,2014,25(10):2418-2419.
[8]秦俊法.中国的中药微量元素研究—Ⅲ.微量元素:中药性效量化的物质基础[J].广东微量元素科学,2011,18(1):1-10.QIN Junfa.The research of trace elements of traditional Chinese medicine in China—Ⅲ.Trace element:material basis of quantifying property and effecacy of traditional Chinese medicine[J].Guangdong Trace Element Science,2011,18(1):1-10.
[9]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:213.Pharmacopoeia Committee.Pharmacopoeia of People's Republic of China:Part1[S].Beijing:The Medicine Science and Technology Press of China,2015:213.
[10]刘长利,尹艳,张淑华,等.中药甘草中微量元素与有效成分相关性研究[J].中国中药杂志,2014,39(17):3335-3338.LIU Changli,YIN Yan,ZHANG Shuhua,et al.Study on correlation between trace elements and active ingredients in Glycyrrhiza Radix et Rhizoma[J].Chinese Journal of Traditional Chinese Medicine,2014,39(17):3335-3338.
[11]马亚兵,王海刚,高海青,等.中药微量元素与其药理的关系研究[J].首都医药,2009,16(22):42-43.MA Yabing,WANG Haigang,GAO Haiqing,et al.Study on the relationship between trace elements and pharmacology in traditional Chinese medicine[J].Capital Medicine,2009,16(22):42-43.
[12]盛良.论化学元素具有寒热温凉四性--论中药四性与现代化学的统一[J].中国中医基础医学杂志,2003,9(1):14-15,29.SHENG Liang.Theory of chemical elements and four properties of traditional Chinese medicine--on the unity of traditional Chinese medicine and modern chemistry[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2003,9(1):14-15,29.
[13]谭晓梅,王新雨,张明明,等.5种贝壳类动物药及其煎出物中微量元素含量测定[J].中国实验方剂学杂志,2011,17(1):61-63.TAN Xiaomei,WANG Xinyu,ZHANG Mingming,et al.Determination of microelement of testacean traditional Chinese medicine[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,17(1):61-63.
[14]李珊珊,孙立卿,周长会,等.治疗高血压病中草药中微量元素的主成分分析[J].南阳理工学院学报,2014,6(6):114-116.LI Shanshan,SUN Liqin,ZHOU Changhui,et al.Principal component analysis of micro-elements in the treatment of hypertension on Chinese herbal medicine[J].Journal of Nanyang Institute of Technology,2014,6(6):114-116.
[15]杨柳,张义生,徐惠芳,等.矿物类中药石膏清热作用研究进展[J].中国药师,2016,19(10):1943-1945.YANG Liu,ZHANG Yisheng,XU Huifang,et al.Research progress in heat-clearing activity of mineral drug Gypsum[J].China Pharmacist,2016,19(10):1943-1945.
[16]罗琴,张锦元,谢启明,等.彝药方杆蕨中微量元素含量与药性关系的研究[J].广东微量元素科学,2013,20(6):23-27.LUO Qin,ZHANG Jinyuan,XIE Qiming,et al.Study of the relationship between trace element content and property of medicine in Yi nationality herbal medicine glaphylopteridopsis erubescens[J].Guangdong Trace Element Science,2013,20(6):23-27.
[17]江胜丽.中药炮制对微量元素的总量和溶出性影响[J].世界最新医学信息文摘,2015,15(41):163.JIANG Shengli.Effect of Chinese medicine processing on total amount and dissolution of trace elements[J].World Latest Medicine Information,2015,15(41):163.
[18]姜威,李晶峰,高久堂,等.石决明的化学成分及药理作用[J].吉林中医药,2015,35(3):272-274.JIANG Wei,LI Jingfeng,GAO Jiutang,et al.Chemical constituents and pharmacological effects of Abalone Shell[J].Jilin Journal of Traditional Chinese Medicine,2015,35(3):272-274.