玉米籽粒含水量性状相关SSR分子标记的筛选和分析
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摘要
籽粒含水量高是影响玉米机收的主要限制性因素之一。目前对于玉米籽粒含水量的内在遗传基础了解的还很少。本研究利用快速脱水自交系Li18和慢速脱水自交系昌7-2组配了252个F2家系群体。通过田间表型分析,分别选取20个低含水量家系和23个高含水量家系构建DNA混池。利用500对SSR标记进行多态性筛选,发现位于玉米第8染色体的标记umc1627和umc1415与籽粒含水量性状紧密相关,两个标记间的遗传距离为2.9 cM。本研究将为快速脱水品种的选育提供理论依据。
High kernel moisture at harvest is one of the main restrictive factors affecting maize mechanical harvesting.Up to now,there has been little study about the genetic basis of kernel moisture in maize.In this research,252 F2 lines were derived from the cross of Li18(quick dehydration rate) and Chang 7-2(slow dehydration rate).20 low kernel moisture lines and 23 high kernel moisture lines were selected respectively to develop two DNA bulks based on the field phenotypic analysis.The polymorphism was screened based on 500 SSR molecular markers,the results of which showed that two SSR markers umc1627 and umc1415 on chromosome 8 were found to be associated with kernel dehydration.The linkage distance between the two markers was 2.9 cM.This study would provide the theoretical basis for the breeding of quick dehydration rate varieties.
High kernel moisture at harvest is one of the main restrictive factors affecting maize mechanical harvesting.Up to now,there has been little study about the genetic basis of kernel moisture in maize.In this research,252 F2 lines were derived from the cross of Li18(quick dehydration rate) and Chang 7-2(slow dehydration rate).20 low kernel moisture lines and 23 high kernel moisture lines were selected respectively to develop two DNA bulks based on the field phenotypic analysis.The polymorphism was screened based on 500 SSR molecular markers,the results of which showed that two SSR markers umc1627 and umc1415 on chromosome 8 were found to be associated with kernel dehydration.The linkage distance between the two markers was 2.9 cM.This study would provide the theoretical basis for the breeding of quick dehydration rate varieties.
引文
Capelle V.,RemouéC.,Moreau L.,Reyss A.,MahéA.,Massonneau A.,Falque M.,Charcosset A.,Thévenot C.,Rogowsky P.,Coursol S.,and Prioul J.L.,2010,QTLs and candidate genes for desiccation and abscisic acid content in maize kernels,BMC Plant Biol.,10:2
Chen H.,Xia F.,Wu X.R.,and Sun Y.H.,2016,The application of bulked segregant analysis in plant gene mapping Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(6):1546-1551(陈浣,夏菲,吴新儒,孙玉合,2016,集群分离分析法在植物基因定位上的应用,基因组学与应用生物学,35(6):1546-1551)
Chen Y.P.,Meng Q.C.,and Yuan J.H.,2008,Identification of the molecular markers linked to the MRDV-resistance locus in maize using SSR-BSA technique,Jiangshu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),24(5):590-594(陈艳萍,孟庆长,袁建华,2008,利用SSR-BSA技术筛选玉米粗缩病抗性基因分子标记,江苏农业学报,24(5)590-594)
Dai L.Q.,Wu L.,Dong Q.S.,Wu N.,Zhang Z.,and Wang P.W.2016,Analysis of genetic variation and correlation of dehydration rate of maize after physiological maturity,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),38(3):261-265(代力强,吴律,董青松,吴楠,张卓,王丕武2016,玉米生理成熟后子粒自然脱水速率的遗传变异与相关分析,吉林农业大学学报,38(3):261-265)
Ding J.Q.,Wang H.W.,Liu Z.F.,Wu Y.J.,Wang W.L.,and Huang C.L.,2012,The research of grain dehydration rate of maize hybrids and inbred lines.Zuowu Zazhi(Crops),5:26-29(丁佳琦,王红武,刘志芳,吴宇锦,王万龙,黄长玲,2012,玉米单交种和自交系生理成熟后子粒脱水速率的研究,作物杂志,5:26-29)
Li F.H.,Guo J.L.,Yu T.,and Shi Z.S.,2012,Comparative study on dehydration rate of kernel among maize hybrids and paren ts with different maturity periods,Yumi Kexue(Journal of Maize Sciences),20(6):17-24(李凤海,郭佳丽,于涛,史振声,2012,不同熟期玉米杂交种及其亲本子粒脱水速率的比较研究,玉米科学,20(6):17-24)
Li J.L.,2014,Preliminary study on dehydration rate of extremely early maturing maize kernels in different recombinant groups,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(3):5-6(李金良,2014,极早熟玉米重组群体籽粒脱水速率差异的初步研究,黑龙江农业科学,(3):5-6)
Liu X.J.,Wang Z.H.,Wang X.,Li T.F.,and Zhang L.,2010,Primary mapping of qtl for dehydration rate of maize kernel after physiological maturing,Zuowu Xuebao(Acta Agronomica Sinica),36(1):47-52(刘显君,王振华,王霞,李庭锋,张林,2010,玉米籽粒生理成熟后自然脱水速率qtl的初步定位,作物学报,36(1):47-52)
Liu Y.Q.,Li M.S.,Li X.H.,Yone H.J.,Yan N.,Zhou Z.Q.,Zhang X.X.,Xia R.P.,Zhang D.G.,Hao Z.F.,Weng J.F.,Bai L.,Shi Z.S.,and Zhang S.H.,2015,Preliminary study on grain filling and dehydration rate of maize hybrids used predominantly in 1970s-2000s,Yumi Kexue(Journal of Maize Sciences),23(1):85-91(刘艳秋,李明顺,李新海,雍洪军,颜娜,周志强,张晓星,夏仁培,张德贵,郝转芳,翁建峰,白丽,史振声,张世煌,2015,1970s~2000s玉米主栽品种灌浆与脱水速率研究,玉米科学,23(1):85-91)
Lu X.L.,Lan J.H.,and Zhang B.S.,2006,Study on ear moisture loss rate in maize,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry),34(2):48-52(吕香玲,兰进好,张宝石,2006,玉米果穗脱水速率的研究,西北农林科技大学学报,34(2):48-52)
Lv X.L.,Zheng K.Z.,Li Y.,Song M.X.,Yan W.,Zhang K.Y.,Li F.H.,and Shi Z.S.,2015,Identification of major QTL for maize gray leaf spot resistance by BSA method,Yumi Kexue(Journal of Maize Sciences),23(5):16-20(吕香玲,郑克志,李元,宋茂兴,闫伟,张旷野,李凤海,史振声,2015,利用BSA法发掘玉米抗灰斑病主效QTL,玉米科学,23(5):16-20)
Michelmore R.W.,Paran I.,and Kesseli R.V.,1991,Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations,Proc.Natl.Acad.Sci.USA,88(21):9828-9832
Qiao J.F.,Li C.,Liu J.B.,Gu L.M.,Xia L.K.,Zhu W.H.,Huang L.,and Xue H.Z.,2015,Relationship between grain water content and seed filling dynamic of different types of natural dehydration maize varieties,Yumi Kexue(Journal of Maize Sciences),23(5):96-101(乔江方,李川,刘京宝,谷利敏,夏来坤,朱卫红,黄璐,薛华政,2015,不同自然脱水类型玉米品种子粒含水率变化与灌浆动态的关系,玉米科学,23(5):96-101)
Rao L.B.,Hu Q.Z.,Yu X.L.,Wang F.Z.,and Lin S.E.,2015,SSRmarker of bolting-related traits in Brassica rapa,Fenzhi Zhiwu Yuzhong(Molecular Plant Breeding),13(8):1786-1793(饶立兵,胡齐赞,余小林,王芳展,林苏娥,2015,大白菜抽薹性状相关SSR分子标记的筛选,分子植物育种,13(8):1786-1793)
Sala R.G.,Andrade F.H.,Camadro E.L.,and Cerono J.C.,2006Quantitative trait loci for grain moisture at harvest and field grain drying rate in maize(Zea mays L.),Theor.Appl.Genet.112(3):462-471
Sala R.G.,Andrade F.H.,and Cerono J.C.,2012,Quantitative trait loci associated with grain moisture at harvest for line per se and testcross performance in maize:a meta-analysis,Euphytica,185(3):429-440
Song W.,Shi Z.,Xing J.,Duan M.,Su A.,Li C.,Zhang R.Y.,Zhao Y.X.,Luo M.J.,Wang J.D.,and Zhao J.R.,2016,Molecular mapping of quantitative trait loci for grain moisture at harvest in maize,Plant Breeding,136:28-32
Wang Z.H.,Zhou F.M.,Guo H.,Wang J.,Jin H.L.,Liu H.X.,,Wang L.F.,Shen Y.F.,Yang M.L.,and Wang B.T.,2015,Research and ana lysis on dehydration rate of maize grain and germplasm improvement tactics,Nongxue Xuebao(Journal of Agriculture),5(7):15-18(王志红,周福民,郭华,王静,靳海蕾,刘海霞,王良发,申亚飞,杨美丽,王帮太,2015,玉米籽粒脱水速率研究分析及种质改良策略,农学学报,5(7):15-18)
Ye Y.S.,Wang X.L.,Li G.,Li Y.,Li Z.,and Wang Y.B.,2015,Research progress on dehydration rate after maize grain physiological mature,Liaoning Nongye Kexue(Liaoning Agricultural Sciences),(3):46-48(叶雨盛,王晓琳,李刚,李洋,李哲,王延波,2015,玉米籽粒生理成熟后脱水速率的研究及应用,辽宁农业科学,(3):46-48)
Chen H.,Xia F.,Wu X.R.,and Sun Y.H.,2016,The application of bulked segregant analysis in plant gene mapping Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(6):1546-1551(陈浣,夏菲,吴新儒,孙玉合,2016,集群分离分析法在植物基因定位上的应用,基因组学与应用生物学,35(6):1546-1551)
Chen Y.P.,Meng Q.C.,and Yuan J.H.,2008,Identification of the molecular markers linked to the MRDV-resistance locus in maize using SSR-BSA technique,Jiangshu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),24(5):590-594(陈艳萍,孟庆长,袁建华,2008,利用SSR-BSA技术筛选玉米粗缩病抗性基因分子标记,江苏农业学报,24(5)590-594)
Dai L.Q.,Wu L.,Dong Q.S.,Wu N.,Zhang Z.,and Wang P.W.2016,Analysis of genetic variation and correlation of dehydration rate of maize after physiological maturity,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),38(3):261-265(代力强,吴律,董青松,吴楠,张卓,王丕武2016,玉米生理成熟后子粒自然脱水速率的遗传变异与相关分析,吉林农业大学学报,38(3):261-265)
Ding J.Q.,Wang H.W.,Liu Z.F.,Wu Y.J.,Wang W.L.,and Huang C.L.,2012,The research of grain dehydration rate of maize hybrids and inbred lines.Zuowu Zazhi(Crops),5:26-29(丁佳琦,王红武,刘志芳,吴宇锦,王万龙,黄长玲,2012,玉米单交种和自交系生理成熟后子粒脱水速率的研究,作物杂志,5:26-29)
Li F.H.,Guo J.L.,Yu T.,and Shi Z.S.,2012,Comparative study on dehydration rate of kernel among maize hybrids and paren ts with different maturity periods,Yumi Kexue(Journal of Maize Sciences),20(6):17-24(李凤海,郭佳丽,于涛,史振声,2012,不同熟期玉米杂交种及其亲本子粒脱水速率的比较研究,玉米科学,20(6):17-24)
Li J.L.,2014,Preliminary study on dehydration rate of extremely early maturing maize kernels in different recombinant groups,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(3):5-6(李金良,2014,极早熟玉米重组群体籽粒脱水速率差异的初步研究,黑龙江农业科学,(3):5-6)
Liu X.J.,Wang Z.H.,Wang X.,Li T.F.,and Zhang L.,2010,Primary mapping of qtl for dehydration rate of maize kernel after physiological maturing,Zuowu Xuebao(Acta Agronomica Sinica),36(1):47-52(刘显君,王振华,王霞,李庭锋,张林,2010,玉米籽粒生理成熟后自然脱水速率qtl的初步定位,作物学报,36(1):47-52)
Liu Y.Q.,Li M.S.,Li X.H.,Yone H.J.,Yan N.,Zhou Z.Q.,Zhang X.X.,Xia R.P.,Zhang D.G.,Hao Z.F.,Weng J.F.,Bai L.,Shi Z.S.,and Zhang S.H.,2015,Preliminary study on grain filling and dehydration rate of maize hybrids used predominantly in 1970s-2000s,Yumi Kexue(Journal of Maize Sciences),23(1):85-91(刘艳秋,李明顺,李新海,雍洪军,颜娜,周志强,张晓星,夏仁培,张德贵,郝转芳,翁建峰,白丽,史振声,张世煌,2015,1970s~2000s玉米主栽品种灌浆与脱水速率研究,玉米科学,23(1):85-91)
Lu X.L.,Lan J.H.,and Zhang B.S.,2006,Study on ear moisture loss rate in maize,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry),34(2):48-52(吕香玲,兰进好,张宝石,2006,玉米果穗脱水速率的研究,西北农林科技大学学报,34(2):48-52)
Lv X.L.,Zheng K.Z.,Li Y.,Song M.X.,Yan W.,Zhang K.Y.,Li F.H.,and Shi Z.S.,2015,Identification of major QTL for maize gray leaf spot resistance by BSA method,Yumi Kexue(Journal of Maize Sciences),23(5):16-20(吕香玲,郑克志,李元,宋茂兴,闫伟,张旷野,李凤海,史振声,2015,利用BSA法发掘玉米抗灰斑病主效QTL,玉米科学,23(5):16-20)
Michelmore R.W.,Paran I.,and Kesseli R.V.,1991,Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations,Proc.Natl.Acad.Sci.USA,88(21):9828-9832
Qiao J.F.,Li C.,Liu J.B.,Gu L.M.,Xia L.K.,Zhu W.H.,Huang L.,and Xue H.Z.,2015,Relationship between grain water content and seed filling dynamic of different types of natural dehydration maize varieties,Yumi Kexue(Journal of Maize Sciences),23(5):96-101(乔江方,李川,刘京宝,谷利敏,夏来坤,朱卫红,黄璐,薛华政,2015,不同自然脱水类型玉米品种子粒含水率变化与灌浆动态的关系,玉米科学,23(5):96-101)
Rao L.B.,Hu Q.Z.,Yu X.L.,Wang F.Z.,and Lin S.E.,2015,SSRmarker of bolting-related traits in Brassica rapa,Fenzhi Zhiwu Yuzhong(Molecular Plant Breeding),13(8):1786-1793(饶立兵,胡齐赞,余小林,王芳展,林苏娥,2015,大白菜抽薹性状相关SSR分子标记的筛选,分子植物育种,13(8):1786-1793)
Sala R.G.,Andrade F.H.,Camadro E.L.,and Cerono J.C.,2006Quantitative trait loci for grain moisture at harvest and field grain drying rate in maize(Zea mays L.),Theor.Appl.Genet.112(3):462-471
Sala R.G.,Andrade F.H.,and Cerono J.C.,2012,Quantitative trait loci associated with grain moisture at harvest for line per se and testcross performance in maize:a meta-analysis,Euphytica,185(3):429-440
Song W.,Shi Z.,Xing J.,Duan M.,Su A.,Li C.,Zhang R.Y.,Zhao Y.X.,Luo M.J.,Wang J.D.,and Zhao J.R.,2016,Molecular mapping of quantitative trait loci for grain moisture at harvest in maize,Plant Breeding,136:28-32
Wang Z.H.,Zhou F.M.,Guo H.,Wang J.,Jin H.L.,Liu H.X.,,Wang L.F.,Shen Y.F.,Yang M.L.,and Wang B.T.,2015,Research and ana lysis on dehydration rate of maize grain and germplasm improvement tactics,Nongxue Xuebao(Journal of Agriculture),5(7):15-18(王志红,周福民,郭华,王静,靳海蕾,刘海霞,王良发,申亚飞,杨美丽,王帮太,2015,玉米籽粒脱水速率研究分析及种质改良策略,农学学报,5(7):15-18)
Ye Y.S.,Wang X.L.,Li G.,Li Y.,Li Z.,and Wang Y.B.,2015,Research progress on dehydration rate after maize grain physiological mature,Liaoning Nongye Kexue(Liaoning Agricultural Sciences),(3):46-48(叶雨盛,王晓琳,李刚,李洋,李哲,王延波,2015,玉米籽粒生理成熟后脱水速率的研究及应用,辽宁农业科学,(3):46-48)