多囊卵巢综合征患者GH、IGF-1水平检测对预测体外受精-胚胎移植结局的价值
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摘要
目的探究多囊卵巢综合征(PCOS)患者生长激素(GH)、胰岛素样生长因子-1(IGF-1)表达水平及其与体外受精-胚胎移植(IVF-ET)结局的关系。方法选取2016年8月至2018年8月本院行IVF-ET的不孕患者92例。其中49例PCOS患者为PCOS组;43例输卵管因素不孕患者为对照组。观察两组卵泡液GH、IGF-1水平及IVF-ET结局;分析卵泡液GH、IGF-1不同水平PCOS患者IVF-ET结局及妊娠与未妊娠患者卵泡液GH、IGF-1水平。结果 PCOS组卵泡液GH、IGF-1水平、获卵数、卵裂数、优质胚胎数、OHSS率均高于对照组,差异有统计学意义(P<0.05)。高GH水平PCOS患者获卵数、卵裂数、优质胚胎数、临床妊娠率高于低GH水平者,高IGF-1水平PCOS患者获卵数、卵裂数、优质胚胎数、临床妊娠率高于低IGF-1水平者,差异有统计学意义(P<0.05)。PCOS妊娠患者卵泡液GH、IGF-1水平均高于未妊娠者,差异有统计学意义(P<0.05)。结论 PCOS患者卵泡液GH、IGF-1水平表达监测对IVF-ET结局具有重要预测价值。
Objective:To investigate the expression levels of growth hormone(GH)and insulin-like growth factor-1(IGF-1)in patients with polycystic ovary syndrome(PCOS)and their relationship with in vitro fertilization-embryo transfer(IVF-ET)outcomes. Methods:A total of 92 infertile patients with IVF-ET were enrolled in our hospital from August 2016 to August 2018. Among them,49 patients with PCOS were in the PCOS group;43 patients with infertility due to tubal factors were in the control group. The levels of GH,IGF-1 and IVF-ET in follicular fluid were observed. The levels of VEGF and IGF-1 in follicular fluid of patients with PCOS in different levels of follicular fluid GH and IGF-1 were analyzed. Results:The levels of GH,IGF-1,number of eggs,number of cleavage,number of high quality embryos and OHSS in follicular fluid of PCOS group were higher than those of the control group(P<0.05). High GH level PCOS patients obtained number of eggs,number of cleavage,number of high quality embryos,clinical pregnancy rate higher than low GH level,high IGF-1 level PCOS patients obtained number of eggs,number of cleavage,number of high quality embryos,high clinical pregnancy rate At low IGF-1 levels,the difference was statistically significant(P<0.05). The levels of GH and IGF-1 in follicular fluid of patients with PCOS were higher than those without pregnancy(P<0.05). Conclusion:The monitoring of GH and IGF-1 expression in follicular fluid of patients with PCOS has important predictive value for IVF-ET outcome.
Objective:To investigate the expression levels of growth hormone(GH)and insulin-like growth factor-1(IGF-1)in patients with polycystic ovary syndrome(PCOS)and their relationship with in vitro fertilization-embryo transfer(IVF-ET)outcomes. Methods:A total of 92 infertile patients with IVF-ET were enrolled in our hospital from August 2016 to August 2018. Among them,49 patients with PCOS were in the PCOS group;43 patients with infertility due to tubal factors were in the control group. The levels of GH,IGF-1 and IVF-ET in follicular fluid were observed. The levels of VEGF and IGF-1 in follicular fluid of patients with PCOS in different levels of follicular fluid GH and IGF-1 were analyzed. Results:The levels of GH,IGF-1,number of eggs,number of cleavage,number of high quality embryos and OHSS in follicular fluid of PCOS group were higher than those of the control group(P<0.05). High GH level PCOS patients obtained number of eggs,number of cleavage,number of high quality embryos,clinical pregnancy rate higher than low GH level,high IGF-1 level PCOS patients obtained number of eggs,number of cleavage,number of high quality embryos,high clinical pregnancy rate At low IGF-1 levels,the difference was statistically significant(P<0.05). The levels of GH and IGF-1 in follicular fluid of patients with PCOS were higher than those without pregnancy(P<0.05). Conclusion:The monitoring of GH and IGF-1 expression in follicular fluid of patients with PCOS has important predictive value for IVF-ET outcome.
引文
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[14]张俊娣,韩艳,李玉洁,等. GnRH拮抗剂对辅助生殖患者血清及卵泡液中IGF-I、IGFBP-I及雌孕激素水平的影响[J].山东医药,2016,56(41):86-88.
[15]吕芳,张保合,陈智英. IVF-ET患者IGFBP-3和IGF-1水平与卵泡发育、卵巢内分泌、胚胎质量及妊娠之间的关系[J].广东医学,2016,37(12):1836-1839.
[2]崔美静,项莹.生长激素释放多肽在多囊卵巢综合征中的研究进展[J].医学综述,2017,23(12):2447-2452.
[3]吴淑芳,余海洋,颜仙.不同表型多囊卵巢综合征患者代谢综合征特征及代谢紊乱发生危险因素[J].医学临床研究,2017,34(8):1552-1555.
[4]Li D,You Y,Bi F F,et al. Autophagy is activated in the ovarian tissue of polycystic ovary syndrome[J]. Reproduction,2017,155(1):REP-17-0499.
[5]徐鑫,李凌云,张静文,等.两种卵泡刺激素在多囊卵巢综合征患者体外受精-胚胎移植中疗效比较[J].生殖医学杂志,2016,25(4):305-309.
[6]李扬璐,阮祥燕,Alfred O. Mueck.多囊卵巢综合征对妊娠结局的影响研究进展[J].首都医科大学学报,2016,37(4):449-453.
[7]蔡贺,张天真,孔令晶,等.生长激素对卵巢低反应患者卵泡液生长激素、胰岛素样生长因子-I及胰岛素样生长因子结合蛋白-3水平的影响[J].中国妇产科临床杂志,2016,17(4):328-332.
[8]李肖肖,王珊,于彩虹,等.生长激素对IVF—ET结局影响的研究进展[J].中国计划生育学杂志,2017,25(2):141-144.
[9]Kucera R,Babuska V,Ulcovagallova Z,et al. Follicular fluid levels of anti-Müllerian hormone,insulin-like growth factor 1 and leptin in women with fertility disorders.[J]. Systems Biology in Reproductive Medicine,2018,64(3):1-4.
[10] Zhuohui Zhong,Fang Li,Yingying Li,et al. Inhibition of microRNA-19b promotes ovarian granulosa cell proliferation by targeting IGF-1 in polycystic ovary syndrome[J]. Molecular Medicine Reports,2018,17(4):4889-4898.
[11]胡勇,谭超.多囊卵巢综合征患者妊娠母血及脐血中胰岛素样生长因子-1水平对妊娠结局的影响[J].实用医学杂志,2016,32(1):73-75.
[12]赵考考,孙秀芹.多囊卵巢综合征患者体外受精胚胎移植助孕后的妊娠结局分析[J].中国优生与遗传杂志,2016,24(5):124-125.
[13] LuoL,WangQ,ChenM,etal.IGF-1andIGFBP-1in peripheral blood and decidua of early miscarriages with euploid embryos:comparison between women with and without PCOS[J].Gynecological Endocrinology,2016,32(7):538-542.
[14]张俊娣,韩艳,李玉洁,等. GnRH拮抗剂对辅助生殖患者血清及卵泡液中IGF-I、IGFBP-I及雌孕激素水平的影响[J].山东医药,2016,56(41):86-88.
[15]吕芳,张保合,陈智英. IVF-ET患者IGFBP-3和IGF-1水平与卵泡发育、卵巢内分泌、胚胎质量及妊娠之间的关系[J].广东医学,2016,37(12):1836-1839.