风箱果叶色变化及呈色机理研究
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摘要
为研究风箱果叶片不同生长发育过程中叶色变化及呈色机理的问题,以风箱果、‘金叶’风箱果、‘紫叶’风箱果为材料,对其叶片从幼嫩到成熟转变过程5个生长阶段的叶部特征和叶色变化进行比较研究,并采用徒手切片法观察叶片中色素空间分布。结果表明,随着3种风箱果的生长,叶片面积均呈逐渐增大的趋势,‘紫叶’风箱果叶面积最大;3种风箱果叶绿素a含量、叶绿素b含量、叶绿素/类胡萝卜素均呈现逐渐升高的趋势,风箱果不同生长阶段叶绿素a含量、叶绿素b含量均显著高于‘紫叶’风箱果和‘金叶’风箱果;3种风箱果叶片色素均主要分布于栅栏组织内,在海绵组织和表皮细胞分布较少。上述结果明确了风箱果叶片在生长发育过程中的叶部特征和叶色表现,为其科学合理应用提供了理论依据。
This paper aims to investigate the leaf color change under different growing stages and coloring mechanism of different Physocarpus varieties.Three Physocarpus amurensis varieties,including P.amurensis,P.amurensis‘Luteus' and P.amurensis‘Diabolo'were used as materials,we investigated the leaf characteristics of five different leaf growth stages from youth to mature.In addition,comparative analysis was conducted on characteristics in terms of leaf color change.We also examined the spatial distribution of anthocyanins glycosides in P.amurensis.The results showed that the leaf areas significantly increased as the plants grew,and P.amurensis‘Diabolo'had the biggest value of leaf area.As the leaf grew,chlorophyll a,chlorophyll b,and the ratio of chlorophyll to carotenoids showed same increasing trend.The contents of chlorophyll a and chlorophyll b of P.amurensis were extremely higher than those of the other two varieties.The leaf anthocyanins glycosides were mainly distributed in the palisade tissue,while lower in the spongy tissue and surface cells.These results above clearly explained leaf characteristics and performance of color change of the three P.amurensis varieties,and provided a theoretical basis for the scientific and reasonable application.
This paper aims to investigate the leaf color change under different growing stages and coloring mechanism of different Physocarpus varieties.Three Physocarpus amurensis varieties,including P.amurensis,P.amurensis‘Luteus' and P.amurensis‘Diabolo'were used as materials,we investigated the leaf characteristics of five different leaf growth stages from youth to mature.In addition,comparative analysis was conducted on characteristics in terms of leaf color change.We also examined the spatial distribution of anthocyanins glycosides in P.amurensis.The results showed that the leaf areas significantly increased as the plants grew,and P.amurensis‘Diabolo'had the biggest value of leaf area.As the leaf grew,chlorophyll a,chlorophyll b,and the ratio of chlorophyll to carotenoids showed same increasing trend.The contents of chlorophyll a and chlorophyll b of P.amurensis were extremely higher than those of the other two varieties.The leaf anthocyanins glycosides were mainly distributed in the palisade tissue,while lower in the spongy tissue and surface cells.These results above clearly explained leaf characteristics and performance of color change of the three P.amurensis varieties,and provided a theoretical basis for the scientific and reasonable application.
引文
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[23]李亚蒙,王庆菊,沈向.四种李属彩叶树种枝叶花色苷分布的解剖研究[J].山东农业大学学报,2006,37(4):489-494.
[24]王振兴,于云飞,陈丽,等.彩叶植物叶片色素组成、结构以及光合特性的研究进展[J].植物生理学报,2016,1:1-7.
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[2]王德芳.北京地区彩叶植物引种栽培研究[J].中国农学通报,2012,19:297-302.
[3]徐志豪,王桂林.彩叶植物引种及其园林应用后评价研究[J].安徽农业科学,2015,2:194-197.
[4]于晓南,张启翔.彩叶植物多彩形成的研究进展[J].园艺学报,2000(S1):533-538.
[5]聂文鑫,徐晓芳,杨林.Na Cl胁迫对3种李属彩叶植物光合特性的影响[J].安徽农业科学,2015,35:243-245.
[6]俞继红.3种彩叶植物的光合特性比较[J].西北林学院学报,2014,4:21-25.
[7]杨帆,李静,姚雪晗,等.2种引种彩叶植物光合日变化及其影响因子研究[J].安徽农业大学学报,2013,05:746-750.
[8]许鑫科,苑兆和,冯立娟,等.彩叶植物叶色表达机制研究进展[J].中国农学通报,2008,11:339-345.
[9]周以良,董世林,聂绍荃.黑龙江树木志[M].哈尔滨:黑龙江科学技术出版社,1986:284-286.
[10]Wang L S,Hashimoto F,Shiraishi A,et al.Coloration and pigmentation of tree peony cultivars of the Northwest of China[J].J Jpn Soc Hort Sci,2000,69(2):233.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-137.
[12]唐前瑞.红檵木遗传多样性及其叶色变化的生理生化研究[D].长沙:湖南农业大学,2001:10-11.
[13]初庆刚,王伟.植物学实验教程[M].北京:高等教育出版社,2011:106-107.
[14]何奕昆,代庆阳,苏学辉.雁来红叶色转变与超微结构及色素含量的关系[J].四川师范学院学报:自然科学版,1995,3:195-198.
[15]黄晓群,赵海新,董春林,等.水稻叶绿素合成缺陷突变体及其生物学研究进展[J].西北植物学报,2005,8:1685-1691.
[16]常青山,陈发棣,滕年军,等.菊花黄绿叶突变体不同类型叶片的叶绿素含量和结构特征比较[J].西北植物学报,2008,9:1772-1777.
[18]唐前瑞,陈德富,陈友云,等.红檵木叶色变化的生理生化研究[J].林业科学,2006,2:111-115.
[19]吴驭帆,于萍,祝遵凌.春季不同叶色鹅耳枥叶片生理生化特性的研究[J].西北农林科技大学学报:自然科学版,2016,5:120-126,132.
[20]吴慧,王爱波,潘一展.彩叶植物叶片色素含量影响因素研究进展[J].北方园艺,2016,8:197-200.
[21]胡永红,秦俊.上海地区秋色叶成因的调查与分析[J].东北林业大学学报,2004,32(5):84-86.
[22]郝峰鸽,杨立峰,周秀梅.4种彩叶植物生长期色素含量研究[J].西北林学院学报,2006,21(6):63-65.
[23]李亚蒙,王庆菊,沈向.四种李属彩叶树种枝叶花色苷分布的解剖研究[J].山东农业大学学报,2006,37(4):489-494.
[24]王振兴,于云飞,陈丽,等.彩叶植物叶片色素组成、结构以及光合特性的研究进展[J].植物生理学报,2016,1:1-7.
[25]葛雨萱,王亮生,周肖红,等.香山黄栌叶色和色素组成的相互关系及时空变化[J].林业科学,2011,4:38-42.