东方百合‘Sorbonne’鳞片离体培养研究
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摘要
以东方百合品种‘Sorbonne’鳞片为外植体,研究不同因子对‘Sorbonne’消毒、诱导、增殖、生根等的影响。结果表明:‘Sorbonne’外层鳞片消毒最为困难,而中层和内层差异不大;最佳消毒方法是外层鳞片用75%酒精处理30 s再用0.1%升汞浸泡18~20 min;中层和内层鳞片用75%酒精处理30 s,再用0.1%升汞浸泡14~16 min,其中不同部位鳞片诱导率从高到低分别为外层>中层>内层,其中外层的诱导率为76.7%;百合鳞片最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,增殖系数为6.8;最佳生根培养基为1/2MS+6-BA 0.5 mg/L+NAA0.2 mg/L,平均生根数为20条。
The bulb scales of Lilium oriental Hybrids ′ Sorbonne′ were used as explants for in vitro culture. The results showed that the best disinfection method of the outer bulb scale explants of ′ Sorbonne′ was 75% ethanol for 30 s,followed by 0.1% Hg Cl218 ~20 min,and the best disinfection method of the middle scale and the inner scale bulb scale explants of ′Sorbonne′ was 75% ethanol for 30 s,followed by 0.1% Hg Cl214~16min. The most difficult was the outer scale disinfection,but difference of disinfection effect of the middle scale and the inner scale was smaller. The scales in different parts had different rate of induction of bulbs as follows :the outer scales>the middle scales>the inner scales. The induction rate of the outer scales was 76.7%. The appropriate medium for multiplication was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L with the multiplication coefficient of 6.8;the best medium for root induction was 1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L and the number of roots per plantlets of 20.
The bulb scales of Lilium oriental Hybrids ′ Sorbonne′ were used as explants for in vitro culture. The results showed that the best disinfection method of the outer bulb scale explants of ′ Sorbonne′ was 75% ethanol for 30 s,followed by 0.1% Hg Cl218 ~20 min,and the best disinfection method of the middle scale and the inner scale bulb scale explants of ′Sorbonne′ was 75% ethanol for 30 s,followed by 0.1% Hg Cl214~16min. The most difficult was the outer scale disinfection,but difference of disinfection effect of the middle scale and the inner scale was smaller. The scales in different parts had different rate of induction of bulbs as follows :the outer scales>the middle scales>the inner scales. The induction rate of the outer scales was 76.7%. The appropriate medium for multiplication was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L with the multiplication coefficient of 6.8;the best medium for root induction was 1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L and the number of roots per plantlets of 20.
引文
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[11]吴英英,许昌慧,唐军荣,等.野生紫斑百合高效离体再生体系的建立[J].云南农业大学学报:自然科学,2013(2):242-246.
[12]李敏,王俊.百合鳞片离体培养的研究[J].北方园艺,2008(2):212-214.
[13]王凤兰,周厚高,黄子锋,等.亚洲百合组培快繁技术研究[J].安徽农业科学,2005(5):820-821.
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[15]郭海滨,雷家军.卷丹百合鳞片及珠芽组织培养研究[J].中国农学通报,2006(2):72-74.
[2]祁宏英,徐洪国,张志.索尔邦百合试管鳞茎的诱导[J].北方园艺,2010(10):178-180.
[3]李守丽,石雷,张金政,等.百合育种研究进展[J].园艺学报,2006,33(1):203-210.
[4]马纯艳.有斑百合叶片的组织培养[J].沈阳师范学院学报:自然科学版,2002,20(3):219-221.
[5]陈学林,李卫锋,黄海涛.荷兰百合索尔邦的离体组织培养[J].安徽农业科学,2007(20):6045-6046.
[6]张淑娟,刘与明.新铁炮百合鳞片培养和快速育苗[J].江苏林业科技,1998(S1):133-134.
[7]唐东芹,黄丹枫,唐克轩,等.东方百合鳞片的组织培养[J].植物生理学通讯,2003(5):450-452.
[8]郭海滨,雷家军,吴淑玲,等.索蚌百合鳞片的组织培养[J].东北林业大学学报,2011(8):30-32.
[9]雷家军,徐莹.垂花百合鳞片离体培养研究[J].东北农业大学学报,2013(1):96-100.
[10]丁兰,刘国安,田卫东,等.新铁炮百合组织培养和快速繁殖研究[J].西北师范大学学报:自然科学版,2001(1):80-82.
[11]吴英英,许昌慧,唐军荣,等.野生紫斑百合高效离体再生体系的建立[J].云南农业大学学报:自然科学,2013(2):242-246.
[12]李敏,王俊.百合鳞片离体培养的研究[J].北方园艺,2008(2):212-214.
[13]王凤兰,周厚高,黄子锋,等.亚洲百合组培快繁技术研究[J].安徽农业科学,2005(5):820-821.
[14]卢娟,于显威.新铁炮百合鳞片组培技术的研究[J].辽宁农业科学,2010(1):23-25.
[15]郭海滨,雷家军.卷丹百合鳞片及珠芽组织培养研究[J].中国农学通报,2006(2):72-74.