不同氨基酸和植物油对替考拉宁产量及组分含量的影响
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摘要
研究了氨基酸和植物油对替考拉宁产量和组分含量的影响。结果发现,与对照相比,在发酵培养基中添加0.05%异亮氨酸和0.05%L-缬氨酸,替考拉宁产量分别提高24.7%和38.2%;在发酵培养基中添加0.50%大豆油和0.50%玉米油,替考拉宁产量分别提高20.4%和28.7%。L-缬氨酸和大豆油在提高替考拉宁产量的同时,对主成分A2-2的影响作用是相反的,L-缬氨酸可以升高A2-2含量,而大豆油则降低A2-2含量。L-缬氨酸和大豆油的联合使用可进一步提高替考拉宁产量,当L-缬氨酸和大豆油添加量分别为0.06%、0.75%时,替考拉宁产量提高47.2%。优化后的配方经过50L发酵罐发酵验证,替考拉宁的平均发酵效价达到5 652U·mL~(-1),各组分含量均符合质量要求,主成分A2-2含量达到53.1%。
We investigated the effects of amino acids and vegetable oils on the yield and component content of Teicoplanin.The results show that,compared with the contast counterparts,the yields of Teicoplanin increase by 24.7% and 38.2% by adding 0.05% isoleucine and 0.05% L-valine,respectively,and the yields of Teicoplanin increase by 20.4% and 28.7% by adding 0.50% soybean oil and 0.50%corn oil,respectively.Furthermore,L-valine and soybean oil have the opposite effects on the content of the main component A2-2 while increasing the yield of Teicoplanin.Adding L-valine in medium can increase the content of A2-2,whereas adding soybean oil in medium can reduce the content of A2-2.L-valine can further increase the yield of Teicoplanin when used in combination with soybean oil.Compared with the contast counterparts,the yield of Teicoplanin increases by 47.2% when the dosages of L-valine and soybean oil are 0.06%and 0.75%,respectively.The results of 50 Lfermentor verification experiment show that the average fermentation titer of Teicoplanin reaches 5 652 U·mL~(-1),the contents of each component meet the quality requirements,and the content of A2-2 reaches 53.1% under the optimized conditions.
We investigated the effects of amino acids and vegetable oils on the yield and component content of Teicoplanin.The results show that,compared with the contast counterparts,the yields of Teicoplanin increase by 24.7% and 38.2% by adding 0.05% isoleucine and 0.05% L-valine,respectively,and the yields of Teicoplanin increase by 20.4% and 28.7% by adding 0.50% soybean oil and 0.50%corn oil,respectively.Furthermore,L-valine and soybean oil have the opposite effects on the content of the main component A2-2 while increasing the yield of Teicoplanin.Adding L-valine in medium can increase the content of A2-2,whereas adding soybean oil in medium can reduce the content of A2-2.L-valine can further increase the yield of Teicoplanin when used in combination with soybean oil.Compared with the contast counterparts,the yield of Teicoplanin increases by 47.2% when the dosages of L-valine and soybean oil are 0.06%and 0.75%,respectively.The results of 50 Lfermentor verification experiment show that the average fermentation titer of Teicoplanin reaches 5 652 U·mL~(-1),the contents of each component meet the quality requirements,and the content of A2-2 reaches 53.1% under the optimized conditions.
引文
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[7]王明蓉,丁洪,胡宇杰.精氨酸和缬氨酸在替考拉宁生物合成中的作用[J].中国抗生素杂志,1996,21(S1):77-80.WANG M R,DING H,HU Y J.The action of arginine and valine in the biosynthesis of Teicoplanin[J].Chinese Journal of Antibiotics,1996,21(S1):77-80.
[8]夏振强,古勇,官家发.糖肽类抗生素替考拉宁研究进展[J].天然产物研究与开发,2005,17(2):247-252.XIA Z Q,GU Y,GUAN J F.Study of Teicoplanin-a glycopcptide antibiotic[J].Natural Product Research and Development,2005,17(2):247-252.
[9] WANG H Y,YANG L,WU K,et al.Rational selection and engineering of exogenous principal sigma factor(σHrdB)to increase Teicoplanin production in an industrial strain of Actinoplanes teichomyceticus[J].Microbial Cell Factories,2014,13(1):10.
[2] ANGELO B,ROSA P,CAROLINA C,et al.Teichomycin A2pure single factors 1,2,3,4and 5and method for their production:US19830502043[P].1983-06-07.
[3] BARNA J C J,WILLIAMS D H,STONE D J M,et al.Structure elucidation of the Teicoplanin antibiotics[J].Journal of American Chemical Society,1984,106(17):4895-4902.
[4] JIN Z,WANG M,CEN P.Production of Teicoplanin by valine analogue-resistant mutant strains of Actinoplanesteichomyceticus[J].Applied Microbiology and Biotechnology,2002,58(1):63-66.
[5]徐波,王明蓉,江世萍,等.鱼蛋白胨和鱼油对替考拉宁产量和组分含量的影响[J].中国抗生素杂志,2004,29(11):641-644.XU B,WANG M R,JIANG S P,et al.The effect of fish peptone and fish oil on the productivity of Teicoplanin by Actinoplanes teichomyceticus[J].Chinese Journal of Antibiotics,2004,29(11):641-644.
[6]张贵民,董其松,方克忠.一种发酵法生产替考拉宁并调控其组分含量的方法:CN 106854670A[P].2017-01-10.
[7]王明蓉,丁洪,胡宇杰.精氨酸和缬氨酸在替考拉宁生物合成中的作用[J].中国抗生素杂志,1996,21(S1):77-80.WANG M R,DING H,HU Y J.The action of arginine and valine in the biosynthesis of Teicoplanin[J].Chinese Journal of Antibiotics,1996,21(S1):77-80.
[8]夏振强,古勇,官家发.糖肽类抗生素替考拉宁研究进展[J].天然产物研究与开发,2005,17(2):247-252.XIA Z Q,GU Y,GUAN J F.Study of Teicoplanin-a glycopcptide antibiotic[J].Natural Product Research and Development,2005,17(2):247-252.
[9] WANG H Y,YANG L,WU K,et al.Rational selection and engineering of exogenous principal sigma factor(σHrdB)to increase Teicoplanin production in an industrial strain of Actinoplanes teichomyceticus[J].Microbial Cell Factories,2014,13(1):10.