深Ⅱ度烫伤后小型猪皮肤愈合的组织病理学研究
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摘要
目的建立猪深Ⅱ度烫伤模型,观察皮肤在不同时间点修复的组织病理学变化。方法通过使用恒温烫伤仪制备小型猪皮肤深Ⅱ度烫伤创面。分别在第1、3、7、14、21、28天观察皮肤创伤愈合的肉眼形态学变化并进行创面愈合率的测定,根据皮肤组织愈合的规律及实验的可操作性,选择性地在第1、14、28天取样,利用HE、Masson染色及免疫组化CD31染色的方法观察皮肤在不同时间点修复的动态病理学变化。结果通过对创面愈合率的测定,雄性猪与雌性猪相比,雄性猪烫伤创面的愈合速率较快。烫伤后皮肤组织经不同染色方法证实损伤已累积到真皮深层,达到深Ⅱ度烫伤的标准。在随后选择的第14天和28天,损伤后皮肤组织分别进行了不同程度的组织修复。深Ⅱ度烫伤28 d后,小型猪皮肤已经基本愈合,但皮肤组织还未完全成熟。除表皮各层相对成熟外,真皮层内还未完全形成瘢痕组织。结论雄性猪与雌性猪相比,雄性猪烫伤创面的愈合速率较快。
Objective To establish the model of porcine deep second degree burn and observe the histopathological changes of the skin at different time points. Method Use the constant tempera turescaler to prepare minipig deep second degree burn. The morphological changes of the wound were observed and the healing rate was measured on the 1 st, 3 rd, 7 th, 14 th, 21 st and 28 th day, respectively. According to the rule of skin tissue healing and the operability of the experiment, scalded tissue was sampled on the 1 st, 14 th and 28 th day, respectively. The histopathological changes of the skin were observed at different time points by HE, Masson staining and immunohistochemical staining with anti-CD31 antibody on the 1 st, 14 th and 28 th day. Result Postburn skin tissue has accumulated into the deep dermis and is confirmed to reach the standard deep second degree burn by using the different methods. On the 14 th and 28 th day of the postburn, different degrees of tissue repair is performed on the damaged skin respectively. On the 28 th day of deep second degree burn, the skin has basically healed, but the skin tissue has not yet fully matured. In addition to the relatively mature layers of the epidermis, the dermis has not yet fully formed the scar tissue.Conclusion The healing rate of male pigs is faster than female pigs.
Objective To establish the model of porcine deep second degree burn and observe the histopathological changes of the skin at different time points. Method Use the constant tempera turescaler to prepare minipig deep second degree burn. The morphological changes of the wound were observed and the healing rate was measured on the 1 st, 3 rd, 7 th, 14 th, 21 st and 28 th day, respectively. According to the rule of skin tissue healing and the operability of the experiment, scalded tissue was sampled on the 1 st, 14 th and 28 th day, respectively. The histopathological changes of the skin were observed at different time points by HE, Masson staining and immunohistochemical staining with anti-CD31 antibody on the 1 st, 14 th and 28 th day. Result Postburn skin tissue has accumulated into the deep dermis and is confirmed to reach the standard deep second degree burn by using the different methods. On the 14 th and 28 th day of the postburn, different degrees of tissue repair is performed on the damaged skin respectively. On the 28 th day of deep second degree burn, the skin has basically healed, but the skin tissue has not yet fully matured. In addition to the relatively mature layers of the epidermis, the dermis has not yet fully formed the scar tissue.Conclusion The healing rate of male pigs is faster than female pigs.
引文
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[9] 李学川,郇京宁,章雄,等.猪深Ⅱ度烫伤早期磨结痂术后创面组织学改变及CK和EGFR表达[J].上海交通大学学报(医学版),2009,29 (5):554-557.
[10] 温馨蔚,崔瑜霞,余蓉.纤维结合蛋白复方制剂在创伤修复模型上的实验研究[J].华西药学杂志,2011,26(5):463-465.
[11] 李敏,姚梅宏,曾玲. PASM-Masson 染色法在肾穿刺活检特殊染色中的应用与体会[J].临床与实验病理学杂志, 2014,30(6): 693-694.
[2] 孙同柱,付小兵,陈伟,等.防疤烧伤膏与京万红促进烫伤创面愈合作用的比较研究[J].中国临床康复,2002, 6(20):3106-3107.
[3] 付小兵,程飚,盛志勇.创面愈合与瘢痕形成的分子学研究[J].中国临床康复,2002,6(4):464-466.
[4] Somboonwong J, Thanamittramaness S, Jariyapongskul A, et al. Therapeutic effects of Aloe vera on cutaneous microcirculation and wound healing in second degree burn model in rats.[J] Journal of the Medical Association of Thailand, 2000,83(4):417-425.
[5] 张静,赵自刚主编.医学科研方法学[M].北京:军事医学科学出版社,2008:103-104.
[6] 付小兵,王德文.现代创伤修复学[M]北京:人民军医出版社,1999:213-233.
[7] 施新猷.医用实验动物学[M].西安:陕西科学技术出版社,1989:64-67.
[8] Singer A, Thode H Jr, McClain S. The effect of octylcyanoacrylate on scarring after burns. [J].Academic Emergency Medicine,2001,8:107-111.
[9] 李学川,郇京宁,章雄,等.猪深Ⅱ度烫伤早期磨结痂术后创面组织学改变及CK和EGFR表达[J].上海交通大学学报(医学版),2009,29 (5):554-557.
[10] 温馨蔚,崔瑜霞,余蓉.纤维结合蛋白复方制剂在创伤修复模型上的实验研究[J].华西药学杂志,2011,26(5):463-465.
[11] 李敏,姚梅宏,曾玲. PASM-Masson 染色法在肾穿刺活检特殊染色中的应用与体会[J].临床与实验病理学杂志, 2014,30(6): 693-694.