摘要
为阐明民族药四数九里香Murraya tetramera Huang的药效物质基础,本研究使用色谱分离技术从其醇提取物中分离纯化得7个咔唑类生物碱和1个甾体类化合物,运用波谱方法鉴定为β-谷甾醇(1)、1-甲基-3-丙酰基咔唑(2)、1-甲氧基-3-乙基咔唑(3)、1-甲氧基-3-甲酰基咔唑(4)、1-甲氧基-3-甲基咔唑(5)、1-羧基甲酯-3-甲基咔唑(6)、1-羧基甲酯-3-乙基咔唑(7)、mananimbine(8)。除了化合物1,其他化合物为首次从该植物中分离得到;化合物2~5、7对SMMC-7721的IC50分别为40. 93±0. 66、46. 03±2. 05、114. 21±5. 67、15. 79±0. 99、185. 1±0. 44。结果表明化合物2~5、7对SMMC-7721显现很好的细胞毒活性。
The Carbazole alkaloids from Murraya tetramera Huang and their cytotoxic activities were investigated in this study. The ethanol extract from Murraya tetramera Huang were separated by chromatographic techniques. The structures of these compounds were identified by1 H NMR,3 C NMR,HSQC,HMBC. they were β-sitosterol(1),1-methyl-3-propionylcarbazole(2),1-methoxy-3-ethylCarbazole(3),1-methoxy-3-formylcarbazole(4),1-methoxy-3-methylcarbazole(5),1-carboxymethylester-3-methylcarbazole(6),1-carboxymethylester-3-ethylcar-bazole(7),manembine(8). All of these compounds were isolated from this plant for the first time except compound(1). The IC50 of compound 2,3,4,5,and 7 against SMMC-7721 was40. 93 ± 0. 66,46. 03 ± 2. 05,114. 21 ± 5. 67,15. 79 ± 0. 99 and 185. 1 ± 0. 44,respectively. The compound(2),(3),(4),(5),(7),showed good cytotoxic activities against SMMC-7721.
引文
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