活化的白细胞粘附分子与早期子宫内膜样子宫内膜癌的细胞侵袭转移及预后的关系研究
|
摘要
目的检测活化的白细胞粘附分子(ALCAM)在早期子宫内膜样子宫内膜癌的表达情况,探讨ALCAM与该病的进展及预后的关系。方法采用免疫组织化学和荧光定量聚合酶链式反应(RT-PCR)检测105例子宫内膜样子宫内膜癌组织和癌旁组织中ALCAM的表达水平,分析不同分期、浸润深度病例ALCAM的表达差异,及其与疾病预后的关系。结果 ALCAM在早期癌组织中的表达量明显高于癌旁组织(P <0. 05),随着肌层浸润深度增加,其表达有增加的趋势。Cox比例风险回归模型分析显示:淋巴结阳性、ALCAM和肿瘤大小是影响早期子宫内膜样子宫内膜癌总体生存时间预后的独立危险因素。结论 ALCAM可能与早期子宫内膜样子宫内膜癌的疾病进展密切相关,其高表达可能促进癌细胞的侵袭转移,可能预示疾病的预后不佳。
Objective To investigate the expression of ALCAM in early endometrial carcinoma and to explore the relationship between ALCAM and progression and prognosis of ALCAM. Methods Immunohistochemical method and RT-PCR were used to detect the expression of ALCAM in 105 cases of endometrioid endometrial carcinoma and adjacent tissues. The relationship between the expression of ALCAM and the progression of disease was analyzed. Results The expression of ALCAM in early cancer tissues was significantly higher than that in adjacent tissues( P < 0. 05). The expression of ALCAM increased with the increase of myometrial invasion depth. Cox proportional hazards regression analysis showed that lymph node positive,ALCAM and tumor size were independent risk factors for the overall survival time of early endometrial endometrial carcinoma. Conclusion ALCAM may be closely related to the progression of early endometrioid endometrial carcinoma,and its high expression may promote the invasion and metastasis of cancer cells,which may indicate the poor prognosis of the disease.
Objective To investigate the expression of ALCAM in early endometrial carcinoma and to explore the relationship between ALCAM and progression and prognosis of ALCAM. Methods Immunohistochemical method and RT-PCR were used to detect the expression of ALCAM in 105 cases of endometrioid endometrial carcinoma and adjacent tissues. The relationship between the expression of ALCAM and the progression of disease was analyzed. Results The expression of ALCAM in early cancer tissues was significantly higher than that in adjacent tissues( P < 0. 05). The expression of ALCAM increased with the increase of myometrial invasion depth. Cox proportional hazards regression analysis showed that lymph node positive,ALCAM and tumor size were independent risk factors for the overall survival time of early endometrial endometrial carcinoma. Conclusion ALCAM may be closely related to the progression of early endometrioid endometrial carcinoma,and its high expression may promote the invasion and metastasis of cancer cells,which may indicate the poor prognosis of the disease.
引文
[1]秦娟,李海英,寇莉.组织β-Catenin mRNA和IGF-1 mRNA检测在子宫内膜癌和子宫内膜息肉疾病鉴别诊断中的应用[J].临床和实验医学杂志,2017,16(5):468-471.
[2]刘超霞,张克强,肖凌志.早期子宫内膜癌手术患者术后复发转移及预后影响因素分析[J].检验医学与临床,2015,12(24):3676-3681.
[3]李明珠,王志启,赵丽君,等.Ⅰ~Ⅱ期子宫内膜癌患者复发及预后的影响因素分析[J].中华妇产科杂志,2014,49(6):455-459.
[4]罗业琳,雷嘉,黄卓华.子宫内膜癌预后因素的Cox回归分析[J].现代肿瘤医学,2015,23(14):2050-2053.
[5]钟立,张秉强.活化白细胞粘附分子与肿瘤[J].黑龙江医学,2014,38(7):745-747.
[6]Kijima N,Hosen N,Kagawa N,et al.CD166/Activatedleukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion[J].Neuro Oncol,2012,14(10):1254-1264.
[7]Al Hilli MM,Podratz KC,Dowdy SC,et al.Risk-scoring system for the individualized prediction of lymphatic dissemination in patients with endometrioid endometrial cancer[J].Gynecol Oncol,2013,131(1):103-108.
[8]Benedetti Panici P,Basile S,Salerno MG,et al.Secondary analyses from a randomized clinical trial:age as the key prognostic factor in endometrial carcinoma[J].Am J Obstet Gynecol,2014,210(4):363.
[9]Piao D,Jiang T,Liu G,et al.Clinical implications of activated leukocyte cell adhesion moleculeexpression in breast cancer[J].Mol Biol Rep,2012,39(1):661-668.
[10]徐珍,彭芝兰,曾俐琴,等.子宫内膜癌淋巴结转移的高危因素分析:附358例报告[J].中华妇幼临床医学杂志(电子版),2014,10(2):165-169.
[11]陈彩虹,林如茵,周云清,等.卵巢子宫内膜样癌组织中活化白细胞黏附分子的表达及临床意义[J].福建医科大学学报,2012,46(4):266-268,282.
[12]Hila KS.Distribution of estrogen and progesterone receptors isoforms in endometrial cancer[J].Diagn Pathol,2014,9:77-85.
[2]刘超霞,张克强,肖凌志.早期子宫内膜癌手术患者术后复发转移及预后影响因素分析[J].检验医学与临床,2015,12(24):3676-3681.
[3]李明珠,王志启,赵丽君,等.Ⅰ~Ⅱ期子宫内膜癌患者复发及预后的影响因素分析[J].中华妇产科杂志,2014,49(6):455-459.
[4]罗业琳,雷嘉,黄卓华.子宫内膜癌预后因素的Cox回归分析[J].现代肿瘤医学,2015,23(14):2050-2053.
[5]钟立,张秉强.活化白细胞粘附分子与肿瘤[J].黑龙江医学,2014,38(7):745-747.
[6]Kijima N,Hosen N,Kagawa N,et al.CD166/Activatedleukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion[J].Neuro Oncol,2012,14(10):1254-1264.
[7]Al Hilli MM,Podratz KC,Dowdy SC,et al.Risk-scoring system for the individualized prediction of lymphatic dissemination in patients with endometrioid endometrial cancer[J].Gynecol Oncol,2013,131(1):103-108.
[8]Benedetti Panici P,Basile S,Salerno MG,et al.Secondary analyses from a randomized clinical trial:age as the key prognostic factor in endometrial carcinoma[J].Am J Obstet Gynecol,2014,210(4):363.
[9]Piao D,Jiang T,Liu G,et al.Clinical implications of activated leukocyte cell adhesion moleculeexpression in breast cancer[J].Mol Biol Rep,2012,39(1):661-668.
[10]徐珍,彭芝兰,曾俐琴,等.子宫内膜癌淋巴结转移的高危因素分析:附358例报告[J].中华妇幼临床医学杂志(电子版),2014,10(2):165-169.
[11]陈彩虹,林如茵,周云清,等.卵巢子宫内膜样癌组织中活化白细胞黏附分子的表达及临床意义[J].福建医科大学学报,2012,46(4):266-268,282.
[12]Hila KS.Distribution of estrogen and progesterone receptors isoforms in endometrial cancer[J].Diagn Pathol,2014,9:77-85.