Folin-Ciocalteu比色法测定石榴皮多酚含量条件的优化研究
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摘要
目的筛选确定Folin-Ciocalteu比色法测定石榴皮提取物中多酚含量的最佳条件。方法本研究选用没食子酸为标准物质,确定测定波长后系统考察多酚含量测定过程中Folin-Ciocalteu试剂(FC试剂)浓度、Na2CO3试剂浓度、反应时间、反应温度等多因素的影响,并对方法学进行考察。结果研究表明,采用Folin-Ciocalteu比色法测定石榴皮提取物多酚含量,取样品液1 ml、40%FC试剂用量5 ml、10%Na2CO3试剂用量4 ml、在20℃避光反应100 min为最佳反应条件,多酚含量与750 nm处吸光度呈良好线性关系;本法加标平均回收率为99.51%,RSD为2.56%。利用优化后的方法测定石榴皮样品中多酚含量为(142.42±5.88)mg/g。结论优化后的Folin-Ciocalteu比色法方便稳定,是检测石榴皮中多酚含量的可靠方法。
Objective To screen and determine the optimal conditions for measurement of polyphenol content in Pomegranate Peel extracts by Folin-Ciocalteu colorimetry. Methods In this study, Gallic Acid was selected as the standard substance. After determining the wavelength of measurement, the effects of Folin-Ciocalteu(FC) reagent concentration, Na_2CO_3 reagent concentration, reaction time and reaction temperature were investigated systematically, and the methodology was also investigated. Results It was showed that the optimum conditions for the measurement of polyphenols in Pomegranate Peel extracts were: 1 ml sample solution, 5 ml 40% FC reagent, 4 ml 10% Na_2CO_3 reagent and protected from light at 20℃ for 100 min. The polyphenol content had a good linear relationship with the absorbance at750 nm. The average recovery rate of this method was 99.51%, and the RSD was 2.56%. The polyphenol content in the Pomegranate leaf sample was determined to be(142.42 ±5.88) mg/g by the optimized method. Conclusion The optimized folin-ciocalteu colorimetric method is convenient and stable, and is reliable to measure polyphenol content in Pomegranate Peel extracts.
Objective To screen and determine the optimal conditions for measurement of polyphenol content in Pomegranate Peel extracts by Folin-Ciocalteu colorimetry. Methods In this study, Gallic Acid was selected as the standard substance. After determining the wavelength of measurement, the effects of Folin-Ciocalteu(FC) reagent concentration, Na_2CO_3 reagent concentration, reaction time and reaction temperature were investigated systematically, and the methodology was also investigated. Results It was showed that the optimum conditions for the measurement of polyphenols in Pomegranate Peel extracts were: 1 ml sample solution, 5 ml 40% FC reagent, 4 ml 10% Na_2CO_3 reagent and protected from light at 20℃ for 100 min. The polyphenol content had a good linear relationship with the absorbance at750 nm. The average recovery rate of this method was 99.51%, and the RSD was 2.56%. The polyphenol content in the Pomegranate leaf sample was determined to be(142.42 ±5.88) mg/g by the optimized method. Conclusion The optimized folin-ciocalteu colorimetric method is convenient and stable, and is reliable to measure polyphenol content in Pomegranate Peel extracts.
引文
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[20]朱彩平,夏德水.Folin-Ciocalteu比色法测定石榴皮提取物中的多酚[J].光谱实验室,2012,29,(3):1356-1360.
[21]李梦颖,李建科,于振,等.石榴多酚的提取、检测和成分分析研究进展[J].食品工业科技,2013,34,(17):384-388.
[22]杜丹丹,李建科.Folin-Ciocalteu比色法测定石榴皮多酚含量条件的优化[J].西北农林科技大学学报,2011,39,(5):190-196.
[23]Prior RL,Wu XL,Schaich K.Standardized methods for the determination of antioxidant capacity phonolics in food and dietary supplements[J].J Agr Food Chem,2005,53,(10):4290-4302.
[2]Wang L,Ho J,Glackin C,et al.Specific pomegranate juice components as potential inhibitors of prostate cancer metastasis[J].Transl Oncol,2012,5,(5):344-355.
[3]Hamid RR,Mohammad A,Seyed NO.A comprehensive review of puncia granatum,(pomegranate)properties in toxicological,pharmacological,cellular and molecular biology researches[J].Iran J Pharm Res,2012,11,(2):385-400.
[4]Eliana HE,Ueda-Nakamura T,Celso VN,et al.Activity of spraydried microparticles containing pomegranate peel extract against Candida albicans[J].Molecules,2012,17(9):10 094-10107.
[5]Iqbal A,Maryam Z,Farrukh A,et al.Bioactive compounds from Punica granatum,Curcuma longa and Zingiber officinale and their therapeutic potential[J].Drug future,2008,33,(4):329-346.
[6]Zarfeshany A,Asgary S,Javanmard SH.Potent health effects of pomegranate[J].Adv Biomed Res,2014,3:100-120.
[7]林勇.石榴皮的化学、药理与临床研究概述[J].中药材,2010,33,(11):1816-1818.
[8]滕碧蔚.石榴皮的研究与应用进展[J].大众科技,2013,15(2):59-61.
[9]热依木古丽·阿布都拉,来海中,马依努尔·拜克力,等.石榴皮化学成分及生物活性研究进展[J].新疆医科大学学报,2013,36,(6):737-740.
[10]杨筱静,赵波,那可,等.石榴皮中多酚类物质的研究进展[J].中国医药工业杂志,2013,44,(5):509-514.
[11]王丽珠,吴棱,姚元根,等.酒石酸亚铁分光光度法测定茶多酚[J].光谱实验室,1997,14,(3):52-54.
[12]王文杰.高锰酸钾滴定法测定茶多酚有关用剂的特性研究[J].福建茶叶,2002,(1):15-17.
[13]刘松,李俊清,廖蓉苏.普鲁士兰法测定胡杨中植物多酚含量[J].林业科技开发,2007,21,(2):42-43.
[14]熊俐,曹新志,王凌云,等.Folin-Ciocalteu法检测菜籽多酚含量的优化研究[J].江苏农业科学,2014,42,(3):255.
[15]赵晓娟,李敏仪,黄桂颖,等.Folin-Ciocalteu法测定苹果醋饮料的总多酚含量[J].食品科学,2013,34,(8):31-35.
[16]李静,聂继云,李海飞,等.Folin-酚法测定水果及其制品中总多酚含量的条件[J].果树学报,2008,25,(1):126-131.
[17]郑雪松,龚钢明,陈彧岩.红景天多酚类物质提取工艺研究[J].食品工业,2009,30(4):31-32.
[18]刘焕云,温志英,张香美,等.葡萄皮中多酚类物质的微波辅助提取技术[J].东北林业大学学报,2009,37,(8):77-78.
[19]中华全国供销合作总社杭州茶叶研究院.GB/T8313-2008茶叶中茶多酚和儿茶素类含量的检测方法[S].北京:标准出版社,2008.
[20]朱彩平,夏德水.Folin-Ciocalteu比色法测定石榴皮提取物中的多酚[J].光谱实验室,2012,29,(3):1356-1360.
[21]李梦颖,李建科,于振,等.石榴多酚的提取、检测和成分分析研究进展[J].食品工业科技,2013,34,(17):384-388.
[22]杜丹丹,李建科.Folin-Ciocalteu比色法测定石榴皮多酚含量条件的优化[J].西北农林科技大学学报,2011,39,(5):190-196.
[23]Prior RL,Wu XL,Schaich K.Standardized methods for the determination of antioxidant capacity phonolics in food and dietary supplements[J].J Agr Food Chem,2005,53,(10):4290-4302.