口蹄疫3ABC非结构蛋白抗体检测试剂盒适用范围探讨
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摘要
为了调查口蹄疫3ABC非机构蛋白抗体在临床诊断健康的动物体内是否存在,以及探索3ABC抗体检测试剂盒的适用范围,设计此试验。随机选取新乡市辖区范围内的猪场4个、牛场12个、羊场9个,采集动物全血,分离血清,利用间接ELISA方法(3ABC-I-ELISA)和荧光PCR方法分别检测免疫过口蹄疫疫苗的健康猪、牛、羊体内非结构蛋白3ABC抗体存在情况和血清中口蹄疫病毒存在情况。结果发现,临床健康动物也会产生口蹄疫3ABC非结构蛋白抗体,且动物日龄越大,产生抗体的比例越高。表明口蹄疫3ABC非结构蛋白抗体不仅在野毒感染动物(包括隐性感染动物)体内产生,而且在健康动物中由于免疫频率的增加和免疫剂量的加大也会产生。因此,口蹄疫3ABC非结构蛋白抗体检测试剂盒只适用于对口蹄疫疫情的初筛,且在日龄较小或免疫口蹄疫疫苗次数较少的动物更加适用。
To confirm whether antibody of foot-and-mouth disease 3 ABC non-structural protein exist in the animals which shown to be healthy in the clinical diagnosis, and to estimate the applied range of 3 ABC antibody detection kit, we designed this study. Whole blood of animals were extracted from randomly selected 4 pig farms, 12 cattle farms and 9 goat farms, and the serum was isolated from the whole blood, then indirect ELISA and qRT-PCR was applied to examine the existence of 3 ABC non-structural protein antibody and the foot-and-mouth disease virus in the vaccine animals. Results showed that clinically healthy animals also produce the 3 ABC non-structural protein antibody, and the production gets higher when the age of animal gets older. 3 ABC non-structural protein antibody is not only produced in the wild-type virus infected or recessive infected animals, but also produced in health animals due to the increase of vaccination frequency and dose. So 3 ABC antibody detection kit is only available for the preliminary screening of the footand-mouth disease, and it is more suitable for the animals of small age or experienced few vaccinations.
To confirm whether antibody of foot-and-mouth disease 3 ABC non-structural protein exist in the animals which shown to be healthy in the clinical diagnosis, and to estimate the applied range of 3 ABC antibody detection kit, we designed this study. Whole blood of animals were extracted from randomly selected 4 pig farms, 12 cattle farms and 9 goat farms, and the serum was isolated from the whole blood, then indirect ELISA and qRT-PCR was applied to examine the existence of 3 ABC non-structural protein antibody and the foot-and-mouth disease virus in the vaccine animals. Results showed that clinically healthy animals also produce the 3 ABC non-structural protein antibody, and the production gets higher when the age of animal gets older. 3 ABC non-structural protein antibody is not only produced in the wild-type virus infected or recessive infected animals, but also produced in health animals due to the increase of vaccination frequency and dose. So 3 ABC antibody detection kit is only available for the preliminary screening of the footand-mouth disease, and it is more suitable for the animals of small age or experienced few vaccinations.
引文
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[2]曹轶梅,刘在新,卢增军,等.口蹄疫病毒非结构蛋白基因3ABC在大肠杆菌中的高效表达[J].畜牧兽医学报,2004,35(1):115-118.
[3]蔡利军.口蹄疫非机构蛋白3ABC单克隆抗体的制备及初步应用[D].武汉:华中农业大学,2007:9-10.
[4]安琪,杨路达,赵兴华,等.河北省部分地区规模化养殖场羊口蹄疫3ABC抗体检测[J].中国动物检疫,2018(,12):14-17.
[5]朱来华,朱可,逢春花,等.免疫猪群口蹄疫非结构蛋白抗体与免疫剂量的关系[J].中国动物检疫,2017,34(11):82-85.