两株分离自疫苗免疫猪场的PRRSV毒株的遗传演化分析
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摘要
猪繁殖与呼吸综合征(PRRS)是严重影响全球养猪业的传染病,疫苗免疫是防控该病的重要措施,但猪繁殖与呼吸综合征病毒(PRRSV)容易发生变异从而降低了免疫预防的效果。本研究从免疫了高致病性PRRS疫苗的发病猪场,分离出2株PRRSV毒株,并测定了其全基因组序列,分别命名为:PRRSV/pig/CHN/JTS/201606和PRRSV/pig/CHN/TG/201711。为了探讨临床中分离得到的这2株PRRSV毒株与该场所使用疫苗之间的关系,我们进行了全基因序列的遗传变异和重组分析,结果显示,这2个毒株都以PRRSV-pig-CHN-TJ-200-610(EU860248.1)疫苗毒株为骨架,其中PRRSV/pig/CHN/JTS/201606毒株在ORF1a蛋白的1429-1522 aa区域存在独特的94 aa的缺失,PRRSV/pig/CHN/TG/201711毒株在1013-1132aa区域存在120 aa的缺失。综上,本研究结果表明,PRRSV疫苗株基因组中核苷酸的突变和缺失产生的新毒株是造成猪场疫苗免疫失败的原因。
Porcine reproductive and respiratory syndrome(PRRS)is one of the most expensive diseases in the global pig industry.Immunoprophylaxis is one of the important measures to prevent and control the disease.However,the protective immunization effects could weaken by the variation of the virus.The genome-wide genetic variation characteristics of two PRRSV strains isolated from highly pathogenic PRRSV strains derived from vaccine strain immunized in different intensive pig farms were analyzed.The two PRRSV strains were named PRRSV/pig/CHN/JTS/201606 and PRRSV/pig/CHN/TG/201711.To further explore the relationship between the isolated virus strains and the vaccine strains used in this field,we then carried out the genetic variation and recombination analysis.And the results showed that PRRSV-pig-CHN-TJ-200610(EU860248.1)strain was the backbone of the three strains.Compared to all the PRRSV strains available at Gen Bank,PRRSV/pig/CHN/JTS/201606 strain had a unique 94 aa deletion in the 1429-1522 aa region of ORF1 a protein.Compared to PRRSV-pig-CHN-TJ-200610,PRRSV/pig/CHN/TG/201711 had a 120 aa deleted at 1013-1132 aa region of ORF1 a protein.In summary,this study showed that the deletions and nucleotide mutations in the whole genome of vaccine like PRRSV strains provide clues for the understanding of immune failure in the PRRSV vaccine immunized pig farms.
Porcine reproductive and respiratory syndrome(PRRS)is one of the most expensive diseases in the global pig industry.Immunoprophylaxis is one of the important measures to prevent and control the disease.However,the protective immunization effects could weaken by the variation of the virus.The genome-wide genetic variation characteristics of two PRRSV strains isolated from highly pathogenic PRRSV strains derived from vaccine strain immunized in different intensive pig farms were analyzed.The two PRRSV strains were named PRRSV/pig/CHN/JTS/201606 and PRRSV/pig/CHN/TG/201711.To further explore the relationship between the isolated virus strains and the vaccine strains used in this field,we then carried out the genetic variation and recombination analysis.And the results showed that PRRSV-pig-CHN-TJ-200610(EU860248.1)strain was the backbone of the three strains.Compared to all the PRRSV strains available at Gen Bank,PRRSV/pig/CHN/JTS/201606 strain had a unique 94 aa deletion in the 1429-1522 aa region of ORF1 a protein.Compared to PRRSV-pig-CHN-TJ-200610,PRRSV/pig/CHN/TG/201711 had a 120 aa deleted at 1013-1132 aa region of ORF1 a protein.In summary,this study showed that the deletions and nucleotide mutations in the whole genome of vaccine like PRRSV strains provide clues for the understanding of immune failure in the PRRSV vaccine immunized pig farms.
引文
[1]ROSSOW K D.Porcine re productive and respi ratory syndrome[J].Veterinary Pathology,1998,35(1):1-20.
[2]WENSVOORT G,TERPSTRA C,POL J M A,et al.Mystery swine disease in The Netherlands:the isolation of Lelystad virus[J].Veterinary Quarterly,1991,13(3):121-130.
[3]COLLINS J E,BENFIELD D A,CHRISTIANSON W T,et al.Isolation of swine infertility and respiratory syndrome virus(isolate ATCC VR-2332)in North America and experimental reproduction of the disease in gnotobiotic pigs[J].Journal of Veterinary Diagnostic Investigation,1992,4(2):117-126.
[4]ZHAO K,YE C,CHANG X B,et al.Importation and recombination are responsible for the latest emergence of highly pathogenic porcinere productive and respiratory syndrome virus in China[J].Journal of Virology,2015,89(20):10712-10716.
[5]ZHOU L,WANG Z,DING Y,et al.NADC30-like strain of porcine reproductive and respiratory syndrome virus,China[J].Emerging Infectious Diseases,2015,21(12):2256-2257.
[6]JIANG Y F,XIA T Q,ZHOU Y J,et al.Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain[J].Veterinary Microbiology,2015,179(3-4):242-249.
[7]BIAN T,SUN Y,HAO M,et al.A recombinant type 2porcine reproductive and respiratory syndrome virus between NADC30-like and a MLV-like:genetic characterization and pathogenicity for piglets[J].Infection,Genetics and Evolution,2017,54:279-286.
[8]ZHANG Z,ZHOU L,GE X,et al.Evolutionary analysis of six isolates of porcine reproductive and respiratory syndrome virus from a single pig farm:MLV-evolved and recombinant viruses[J].Infection,Genetics and Evolution,2018,66:111-119.
[9]ZHAO H,HAN Q,ZHANG L,et al.Emergence of mosaic recombinant strains potentially associated with vaccine JXA1-R and predominant circulating strains of porcine reproductive and respiratory syndrome virus in different provinces of China[J].Virology Journal,2017,14(1):67.
[10]ZHANG Q,JIANG P,SONG Z,et al.Pathogenicity and antigenicity of a novel NADC30-like strain of porcine repro-ductive and respiratory syndrome virus emerged in China[J].Veterinary Microbiology,2016,197:93-101.
[11]LI C,ZHUANG J,WANG J,et al.Outbreak investigation ofNADC30-like PRRSV in South-East China[J].Transboundary and Emerging Diseases,2016,63(5):474-479.
[12]SUN Z,WANG J,BAI X,et al.Pathogenicity comparison be-tween highly pathogenic and NADC30-like porcine reproductive and respiratory syndrome virus[J].Archives of Virology,2016,161(8):2257-2261.
[13]YUAN S,NELSEN C J,MURTAUGH M P,et al.Recombination between North American strains of porcine reproductive and respiratory syndrome virus[J].Virus Research,1999,61(1):87-98.
[14]LENG X,LI Z,XIA M P,et al.Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation[J].Veterinary Microbiology,2012,157(1-2):50-60.
[15]ZHANG Q Y,XU X J,YOU S M,et al.Emerging of two new subgenotypes of porcine reproductive and respiratory syndrome viruses in Southeast China[J].Microb Pathogenesis,2016,97:27-33.
[16]隋修馄,侯绍华,林伟东,等.新的高致病性PRRSV毒株FZ16A的分子特征及其致病性的研究[J].中国兽医科学,2018,48(12):1511-1521.SUI Xiu-kun,HOU Shao-hua,LIN Wei-dong,et al.Molecular and pathogenicity analyses of a new highly pathogenic porcine reproductive and respiratory syndrome virus strain FZ16A[J].Chinese Veterinary Science,2018,48(12):1511-1521.(in Chinese)
[2]WENSVOORT G,TERPSTRA C,POL J M A,et al.Mystery swine disease in The Netherlands:the isolation of Lelystad virus[J].Veterinary Quarterly,1991,13(3):121-130.
[3]COLLINS J E,BENFIELD D A,CHRISTIANSON W T,et al.Isolation of swine infertility and respiratory syndrome virus(isolate ATCC VR-2332)in North America and experimental reproduction of the disease in gnotobiotic pigs[J].Journal of Veterinary Diagnostic Investigation,1992,4(2):117-126.
[4]ZHAO K,YE C,CHANG X B,et al.Importation and recombination are responsible for the latest emergence of highly pathogenic porcinere productive and respiratory syndrome virus in China[J].Journal of Virology,2015,89(20):10712-10716.
[5]ZHOU L,WANG Z,DING Y,et al.NADC30-like strain of porcine reproductive and respiratory syndrome virus,China[J].Emerging Infectious Diseases,2015,21(12):2256-2257.
[6]JIANG Y F,XIA T Q,ZHOU Y J,et al.Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain[J].Veterinary Microbiology,2015,179(3-4):242-249.
[7]BIAN T,SUN Y,HAO M,et al.A recombinant type 2porcine reproductive and respiratory syndrome virus between NADC30-like and a MLV-like:genetic characterization and pathogenicity for piglets[J].Infection,Genetics and Evolution,2017,54:279-286.
[8]ZHANG Z,ZHOU L,GE X,et al.Evolutionary analysis of six isolates of porcine reproductive and respiratory syndrome virus from a single pig farm:MLV-evolved and recombinant viruses[J].Infection,Genetics and Evolution,2018,66:111-119.
[9]ZHAO H,HAN Q,ZHANG L,et al.Emergence of mosaic recombinant strains potentially associated with vaccine JXA1-R and predominant circulating strains of porcine reproductive and respiratory syndrome virus in different provinces of China[J].Virology Journal,2017,14(1):67.
[10]ZHANG Q,JIANG P,SONG Z,et al.Pathogenicity and antigenicity of a novel NADC30-like strain of porcine repro-ductive and respiratory syndrome virus emerged in China[J].Veterinary Microbiology,2016,197:93-101.
[11]LI C,ZHUANG J,WANG J,et al.Outbreak investigation ofNADC30-like PRRSV in South-East China[J].Transboundary and Emerging Diseases,2016,63(5):474-479.
[12]SUN Z,WANG J,BAI X,et al.Pathogenicity comparison be-tween highly pathogenic and NADC30-like porcine reproductive and respiratory syndrome virus[J].Archives of Virology,2016,161(8):2257-2261.
[13]YUAN S,NELSEN C J,MURTAUGH M P,et al.Recombination between North American strains of porcine reproductive and respiratory syndrome virus[J].Virus Research,1999,61(1):87-98.
[14]LENG X,LI Z,XIA M P,et al.Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation[J].Veterinary Microbiology,2012,157(1-2):50-60.
[15]ZHANG Q Y,XU X J,YOU S M,et al.Emerging of two new subgenotypes of porcine reproductive and respiratory syndrome viruses in Southeast China[J].Microb Pathogenesis,2016,97:27-33.
[16]隋修馄,侯绍华,林伟东,等.新的高致病性PRRSV毒株FZ16A的分子特征及其致病性的研究[J].中国兽医科学,2018,48(12):1511-1521.SUI Xiu-kun,HOU Shao-hua,LIN Wei-dong,et al.Molecular and pathogenicity analyses of a new highly pathogenic porcine reproductive and respiratory syndrome virus strain FZ16A[J].Chinese Veterinary Science,2018,48(12):1511-1521.(in Chinese)