上海市宝山区2015—2018年腹泻病人沙门菌血清分型和分子分型
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摘要
目的揭示上海市宝山区腹泻病人分离沙门菌的血清型和分子分型分布特征。方法对2015—2018年分离的118株沙门菌进行血清分型和脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)分型,通过构建聚类树揭示不同血清型菌株的分子分型特征。结果 118株沙门菌分为25种血清型,前三位为肠炎沙门菌(45株,38.1%)、鼠伤寒沙门菌(26株,22.0%)、伦敦沙门菌(11株,9.3%)。其余22种血清型分别只包含1～3株菌。118株沙门菌通过PFGE分为71个PFGE带型,每个带型分别包含1～19株菌。45株肠炎沙门菌分为8个PFGE带型,相似度为75.0%～100.0%,在聚类树上形成一个簇;26株鼠伤寒沙门菌分为21个PFGE带型,相似度为48.0%～100.0%,在聚类树上形成两个簇;11株伦敦沙门菌分为8个PFGE带型,相似度为80.0%～100.0%,在聚类树上形成一个簇。结论肠炎沙门菌和鼠伤寒沙门菌是上海市宝山区腹泻病人沙门菌优势血清型,但近年伦敦沙门菌有增多的趋势。肠炎沙门菌在本地区传播流行过程中的遗传变异相对比较保守,鼠伤寒沙门菌基因型多态性大,而且存在两个克隆。
Objective To reveal the distribution characteristics of serotypes and molecular typing of Salmonella isolated from diarrhea patients in Baoshan District of Shanghai. Methods Serotyping and pulsed-field gel electrophoresis(PFGE) of118 Salmonella strains isolated between 2015 and 2018 were carried out, and molecular subtyping characteristics of different serotypes were revealed by constructing cluster tree. Results One hundred and eighteen Salmonella strains were divided into25 serotypes. The top three were Salmonella enteritidis(45 strains, 38.1%), Salmonella typhimurium(26 strains, 22.0%) and Salmonella london(11 strains, 9.3%). The remaining 22 serotypes contained only 1-3 strains respectively. One hundred and eighteen Salmonella strains were divided into 71 PFGE types, and each type contained 1-19 strains respectively. Forty-five strains of S. enteritidis were divided into eight PFGE types with similarity of 75.0%-100.0%, forming a cluster on the cluster tree; 26 strains of S. typhimurium were divided into 21 PFGE types with similarity of 48.0%-100.0%, forming two clusters on the cluster tree; 11 strains of S. london were divided into eight PFGE types with similarity of 80.0%-100.0%, forming one cluster on the cluster tree. Conclusion S. enteritidis and S. typhimurium are the predominant serotypes of Salmonella in diarrhea patients in Baoshan District of Shanghai, but in recent years, there is an increasing trend of S. london. The genetic variation of S. enteritidis is relatively conservative during its transmission in this area. The genotype of S. typhimurium is highly polymorphic, and there are two clones.
Objective To reveal the distribution characteristics of serotypes and molecular typing of Salmonella isolated from diarrhea patients in Baoshan District of Shanghai. Methods Serotyping and pulsed-field gel electrophoresis(PFGE) of118 Salmonella strains isolated between 2015 and 2018 were carried out, and molecular subtyping characteristics of different serotypes were revealed by constructing cluster tree. Results One hundred and eighteen Salmonella strains were divided into25 serotypes. The top three were Salmonella enteritidis(45 strains, 38.1%), Salmonella typhimurium(26 strains, 22.0%) and Salmonella london(11 strains, 9.3%). The remaining 22 serotypes contained only 1-3 strains respectively. One hundred and eighteen Salmonella strains were divided into 71 PFGE types, and each type contained 1-19 strains respectively. Forty-five strains of S. enteritidis were divided into eight PFGE types with similarity of 75.0%-100.0%, forming a cluster on the cluster tree; 26 strains of S. typhimurium were divided into 21 PFGE types with similarity of 48.0%-100.0%, forming two clusters on the cluster tree; 11 strains of S. london were divided into eight PFGE types with similarity of 80.0%-100.0%, forming one cluster on the cluster tree. Conclusion S. enteritidis and S. typhimurium are the predominant serotypes of Salmonella in diarrhea patients in Baoshan District of Shanghai, but in recent years, there is an increasing trend of S. london. The genetic variation of S. enteritidis is relatively conservative during its transmission in this area. The genotype of S. typhimurium is highly polymorphic, and there are two clones.
引文
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[11]李颖,张爽,王彦波,等.2014-2017年北京市顺义区腹泻病例中沙门菌流行特征与分子分型特征分析[J].疾病监测,2018,33(10):803-808.
[12]孙立珍,孙立平,丁淑芹.北京地区感染性腹泻病原菌监测[J].热带医学杂志,2018,18(1):97-103.
[13]张晶,张欣强,侯水平,等.广州市566株腹泻病例沙门菌的血清型分布及耐药性分析[J].实用预防医学,2016,23(5):616-617,623.
[14]张新峰,王蕾,徐贵永,等.泰安市感染性腹泻非伤寒沙门菌耐药监测及分型研究[J].中国人兽共患病学报,2017,33(4):337-342.
[15]游旅,何寻面,韦小瑜,等.2013-2014年贵阳市感染性腹泻监测病例中沙门菌的病原学监测分析[J].中国人兽共患病学报,2018,34(8):712-715.
[16]沙丹,李泓,管红霞,等.无锡市腹泻病人沙门菌的病原学特征及分子分型研究[J].中国人兽共患病学报,2017,33(4):378-381.2019-02-21
[2]王鑫.2010-2014年我国感染性腹泻病原流行特征研究[D].北京:北京协和医学院,2016.
[3]江晓,杜雪飞,叶艳华,等.2015-2016年南京地区儿童感染性腹泻病原学监测结果分析[J].实用预防医学,2018,25(12):1508-1510.
[4]FERRARIR G,PANZENHAGENP H N,CONTE-JUNIORC A.Phenotypic and genotypic eligible methods for salmonellatyphimurium source tracking[J].Front Microbiol,2017,8:2587.
[5]方艳梅,杨春晓,张丽荣,等.珠海市2012-2014年腹泻病例沙门菌血清型分布和耐药情况分析[J].华南预防医学,2017,43(5):474-477.
[6]林一曼,石晓路,李迎慧,等.2012-2013年深圳市非伤寒沙门菌血清型分布、耐药性及PFGE分型研究[J].热带医学杂志,2018,18(1):79-81,96.
[7]JENSENM B F,SCHJ?RRING S,BJ?RKMANJ T,et al.External quality assessment for molecular typing of Salmonella 2013-2015:Performance of the European national public health reference laboratories[J].Eur J Clin Microbiol Infect Dis,2017,36(10):1923-1932.
[8]GRIMONT P A D,WEILL F X.Antigenic formulae of the Salmonella serovars,WHO Collaborating Centre for Reference and Research on Salmonella[M],Paris,France:2007.
[9]RIBOTE M,FAIRM A,GAUTOM R,et al.Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7,Salmonella,and Shigella for PulseNet[J].Foodborne Pathog Dis,2006,3(1):59-67.
[10]DICEL R.Measures of the amount of ecologic association between species[J].Ecology,1945,26(3):297-302.
[11]李颖,张爽,王彦波,等.2014-2017年北京市顺义区腹泻病例中沙门菌流行特征与分子分型特征分析[J].疾病监测,2018,33(10):803-808.
[12]孙立珍,孙立平,丁淑芹.北京地区感染性腹泻病原菌监测[J].热带医学杂志,2018,18(1):97-103.
[13]张晶,张欣强,侯水平,等.广州市566株腹泻病例沙门菌的血清型分布及耐药性分析[J].实用预防医学,2016,23(5):616-617,623.
[14]张新峰,王蕾,徐贵永,等.泰安市感染性腹泻非伤寒沙门菌耐药监测及分型研究[J].中国人兽共患病学报,2017,33(4):337-342.
[15]游旅,何寻面,韦小瑜,等.2013-2014年贵阳市感染性腹泻监测病例中沙门菌的病原学监测分析[J].中国人兽共患病学报,2018,34(8):712-715.
[16]沙丹,李泓,管红霞,等.无锡市腹泻病人沙门菌的病原学特征及分子分型研究[J].中国人兽共患病学报,2017,33(4):378-381.2019-02-21