荧光定量PCR检测解脲支原体沙眼衣原体及淋病奈瑟氏菌结果分析
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摘要
目的研究衡阳地区泌尿生殖道炎症患者解脲支原体(UU)、沙眼衣原体(CT)、淋病奈瑟氏菌(NG)三种性传播疾病病原体的感染状况,探讨这些病原体与性别和年龄的相互关系,为临床诊断预防提供依据。方法采用荧光定量聚合酶链式反应(FQ-PCR)对我院2 182例患者进行UU、CT和NG核酸检测。结果 2 182例患者中,UU、NG及CT阳性率分别为61.94%(1 245/2 010)、24.22%(39/161)、7.82%(77/985),三者之间差异均有统计学意义。混合感染以UU+CT二重感染阳性率最高(9.58%)。女性的UU感染率(63.51%)明显高于男性(36.97%),男性的CT及NG感染率(分别为14.75%和40.00%)明显高于女性(分别为7.36%和13.54%),差异均有统计学意义。0～20岁的CT及NG感染率(分别为25.00%和64.29%)明显高于其他年龄段,差异均有统计学意义;UU阳性率(69.41%)高于其他年龄段,但无统计学差异,而21～40岁是三种病原体感染的主要人群。UU以妇产科门诊阳性率最高,而CT及NG的阳性率以泌尿外科门诊和皮肤科门诊最高,差异均有统计学意义。结论本地区泌尿生殖道患者UU感染率最高并且女性感染率高于男性,而男性的CT及NG感染率高于女性。三种病原体感染主要集中于青壮年,而0～20岁人群感染率高于其他年龄段。三种病原体感染率在科室间同样存在一定的差异。
Objective To investigate the infection status of ureaplasma urealyticum(UU), chlamydozoa trachomatis(CT) and neisseria gonorrhoeae(NG) and explore the correlation among the pathogens, sex and age in Hengyang so as to provide references for clinical diagnosis and prevention. Methods Nucleic acid assay of UU, CT and NG were detected in 2 182 patients by fluorescence quantitative PCR(FQ-PCR). Results The positive rates of UU, NG and CT were 61.94%(1 245/2 010), 24.22%(39/161), and 7.82%(77/985). There were statistical differences between the three groups. UU and CT mixed infection was the highest(9.58%). The infection rate of UU was significantly higher in female group(63.51%) than in male group(36.97%), while it was lower in case of CT(7.36% and 14.75%) and NG infection(13.54% and 40.00%). The differences were statistically significant. The incidences of CT and NG in age group between 0-20 years(25.00% and 64.29%) were obviously higher than other age groups, with the differences statistically significant. Although the infection rate of UU(69.41%)was higher, without statistical differences among different age groups. The age group between 21-40 years was the main group infected with UU, NG and CT. UU positive rate was the highest in the gynecology and obstetrics clinic, while CT and NG positive rate was the highest in urology and dermatology clinic. There were statistical differences among those indicators. Conclusion The UU infection rate of urogenital tract patients is the highest and the female infection rate is higher than the male in this area, while CT and NG infection rates are higher in male than in female. The infection rates of the three pathogens are mainly in young adults, while those indicators between 0-20 years old are higher than other age groups. The infection rates of the three pathogens are also different among departments.
Objective To investigate the infection status of ureaplasma urealyticum(UU), chlamydozoa trachomatis(CT) and neisseria gonorrhoeae(NG) and explore the correlation among the pathogens, sex and age in Hengyang so as to provide references for clinical diagnosis and prevention. Methods Nucleic acid assay of UU, CT and NG were detected in 2 182 patients by fluorescence quantitative PCR(FQ-PCR). Results The positive rates of UU, NG and CT were 61.94%(1 245/2 010), 24.22%(39/161), and 7.82%(77/985). There were statistical differences between the three groups. UU and CT mixed infection was the highest(9.58%). The infection rate of UU was significantly higher in female group(63.51%) than in male group(36.97%), while it was lower in case of CT(7.36% and 14.75%) and NG infection(13.54% and 40.00%). The differences were statistically significant. The incidences of CT and NG in age group between 0-20 years(25.00% and 64.29%) were obviously higher than other age groups, with the differences statistically significant. Although the infection rate of UU(69.41%)was higher, without statistical differences among different age groups. The age group between 21-40 years was the main group infected with UU, NG and CT. UU positive rate was the highest in the gynecology and obstetrics clinic, while CT and NG positive rate was the highest in urology and dermatology clinic. There were statistical differences among those indicators. Conclusion The UU infection rate of urogenital tract patients is the highest and the female infection rate is higher than the male in this area, while CT and NG infection rates are higher in male than in female. The infection rates of the three pathogens are mainly in young adults, while those indicators between 0-20 years old are higher than other age groups. The infection rates of the three pathogens are also different among departments.
引文
[1] 崔辰莹.实时荧光定量聚合酶链反应检测泌尿生殖道感染病原体结果分析[J].山西医药杂志,2013,42(1):97-98.
[2] Fr?lund M,Wikstr?m A,Lidbrink P,et al.The bacterial microbiota in first-void urine from men with and without idiopathic urethritis[J].PLoS One,2018,13(7):e0201380.
[3] 徐丽玲,顾伟明,杨阳,等.1996—2003年上海市某医院泌尿生殖道分泌物标本性病检测结果分析[J].中国艾滋病性病,2005,11(2):121-124.
[4] 蓝丽娜,吴肖冰,张春来,等.深圳市2008—2016年生殖道沙眼衣原体感染的流行病学分析[J].中国艾滋病性病,2017,23(10):947-949.
[5] Del Prete R,Ronga L,Lestingi M,et al.Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia,a region of Southern Italy[J].Infection,2017,45(4):469-477.
[6] 陈定华.衣原体、支原体感染与宫颈糜烂的关系及并发症(附400例临床报告)[J].皮肤病与性病,2010,32(1):51-52.
[7] 丁大朋,方丽平.应用荧光定量PCR与常规方法检测解脲脲原体、沙眼衣原体的比较[J].医学与哲学,2015,36(519):57-59.
[8] 张欠欠,仵恒立,胡军婷,等.荧光定量PCR检测淋球菌、沙眼衣原体和解脲脲原体结果分析[J].中国人兽共患病学报,2016,32(3):312-316.
[9] 刘秀珍,吴旗,王晓梅,等.荧光定量聚合酶链反应对女性生殖道感染解脲脲支原体及沙眼衣原体检测分析[J].中华医学感染学杂志,2010,20(24):4038-4040.
[10] 程家明,郑定容.淋球菌、沙眼衣原体和解脲脲原体混合感染临床分析[J].医学信息,2011,24(9):2612-2613.
[11] Kong F,Ma Z,James G,et al.Species Identification and Subtyping of Ureaplasma parvum and Ureaplasma urealyticum Using PCR-Based Assays[J].J Clin Microbiol,2000,38(3):1175-1179.
[12] Ju Z,Yane G,Ding L,et al.Asymmetric GP5+/6+ PCR and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples[J].J Clin Virol,2009,44(2):106-110.
[13] 周宁,夏建晖,郭燕,等.天津市2008—2013年5种STD种的流行病学分析[J].中国艾滋病性病,2015,21(3):213-216.
[14] 张新生,李琼,郑兆敏,等.女性生殖道性传播疾病病原体混合感染的检测及意义[J].中华医学感染学杂志,2017(3):281-282.
[15] 董晓娟,何婵凤,王妍.孕妇生殖道沙眼衣原体与解脲脲支原体感染对胚胎停育的影响分析[J].中华医学感染学杂志,2016,26(8):1868-1870.
[16] 方菊,曹正英,吉文倩,等.医院非淋球菌性尿道炎患者支原体感染和耐药状况研究[J].中华医院感染学杂志,2017,27(3):510-513.
[17] West SK,Moncada J,Munoz B,et al.Is there evidence for resistance of ocular Chlamydia trachomatis to azithromycin after mass treatment for trachoma control[J].J Infect Dis,2014,210(1):65-71.
[2] Fr?lund M,Wikstr?m A,Lidbrink P,et al.The bacterial microbiota in first-void urine from men with and without idiopathic urethritis[J].PLoS One,2018,13(7):e0201380.
[3] 徐丽玲,顾伟明,杨阳,等.1996—2003年上海市某医院泌尿生殖道分泌物标本性病检测结果分析[J].中国艾滋病性病,2005,11(2):121-124.
[4] 蓝丽娜,吴肖冰,张春来,等.深圳市2008—2016年生殖道沙眼衣原体感染的流行病学分析[J].中国艾滋病性病,2017,23(10):947-949.
[5] Del Prete R,Ronga L,Lestingi M,et al.Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia,a region of Southern Italy[J].Infection,2017,45(4):469-477.
[6] 陈定华.衣原体、支原体感染与宫颈糜烂的关系及并发症(附400例临床报告)[J].皮肤病与性病,2010,32(1):51-52.
[7] 丁大朋,方丽平.应用荧光定量PCR与常规方法检测解脲脲原体、沙眼衣原体的比较[J].医学与哲学,2015,36(519):57-59.
[8] 张欠欠,仵恒立,胡军婷,等.荧光定量PCR检测淋球菌、沙眼衣原体和解脲脲原体结果分析[J].中国人兽共患病学报,2016,32(3):312-316.
[9] 刘秀珍,吴旗,王晓梅,等.荧光定量聚合酶链反应对女性生殖道感染解脲脲支原体及沙眼衣原体检测分析[J].中华医学感染学杂志,2010,20(24):4038-4040.
[10] 程家明,郑定容.淋球菌、沙眼衣原体和解脲脲原体混合感染临床分析[J].医学信息,2011,24(9):2612-2613.
[11] Kong F,Ma Z,James G,et al.Species Identification and Subtyping of Ureaplasma parvum and Ureaplasma urealyticum Using PCR-Based Assays[J].J Clin Microbiol,2000,38(3):1175-1179.
[12] Ju Z,Yane G,Ding L,et al.Asymmetric GP5+/6+ PCR and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples[J].J Clin Virol,2009,44(2):106-110.
[13] 周宁,夏建晖,郭燕,等.天津市2008—2013年5种STD种的流行病学分析[J].中国艾滋病性病,2015,21(3):213-216.
[14] 张新生,李琼,郑兆敏,等.女性生殖道性传播疾病病原体混合感染的检测及意义[J].中华医学感染学杂志,2017(3):281-282.
[15] 董晓娟,何婵凤,王妍.孕妇生殖道沙眼衣原体与解脲脲支原体感染对胚胎停育的影响分析[J].中华医学感染学杂志,2016,26(8):1868-1870.
[16] 方菊,曹正英,吉文倩,等.医院非淋球菌性尿道炎患者支原体感染和耐药状况研究[J].中华医院感染学杂志,2017,27(3):510-513.
[17] West SK,Moncada J,Munoz B,et al.Is there evidence for resistance of ocular Chlamydia trachomatis to azithromycin after mass treatment for trachoma control[J].J Infect Dis,2014,210(1):65-71.