聚缬氨酸修饰电极法测定食品中2,6-二叔丁基对甲苯酚
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摘要
目的建立聚缬氨酸修饰电极法测定食品中2,6-二叔丁基对甲苯酚含量的分析方法。方法对循环伏安法制备聚缬氨酸修饰电极的聚合条件进行了优化,并研究了2,6-二叔丁基对甲苯酚在此电极上的电化学行为。从测定pH、电位、扫描速率等方面,对聚缬氨酸修饰电极法测定饼干中2,6-二叔丁基对甲苯酚含量时的条件进行了优化。结果在pH=4.0的磷酸盐缓冲溶液中,2,6-二叔丁基对甲苯酚的氧化峰电流与其浓度在2.0×10~(-7)～4.0×10~(-5)mol/L范围线性关系良好,线性方程:ipa=1.27×10~(-7)+0.23c,相关系数r为0.9962,检出限为4.0×10~(-8) mol/L。结论本方法简单、快速、灵敏,适用于测定食品中2,6-二叔丁基对甲苯酚的含量。
Objective To establish a method for the determination of butylated hydroxytoluene in food by poly(valine) modified electrode. Methods The polymerization conditions of poly(valine) modified electrode prepared by cyclic voltammetry were optimized, and the electrochemical behavior of butylated hydroxytoluene on this electrode was studied. The conditions for determination of butylated hydroxytoluene content in biscuits by poly(valine) modified electrode method were optimized from the aspects of pH, potential and scanning rate. Results In the pH 4.0 phosphate buffer solution, the oxidation peak current and concentration of butylated hydroxytoluene had a good linear relationship in the range of 2.0×10~(-7)-4.0×10~(-5) mol/L. The linear equation was ipa=1.27×10~(-7)+0.23 c, the correlation coefficient(r) was 0.9962, and the limit of detection was 4.0×10~(-8) mol/L. Conclusion This method is simple, rapid and sensitive, which is suitable for the determination of butylated hydroxytoluene content in food.
Objective To establish a method for the determination of butylated hydroxytoluene in food by poly(valine) modified electrode. Methods The polymerization conditions of poly(valine) modified electrode prepared by cyclic voltammetry were optimized, and the electrochemical behavior of butylated hydroxytoluene on this electrode was studied. The conditions for determination of butylated hydroxytoluene content in biscuits by poly(valine) modified electrode method were optimized from the aspects of pH, potential and scanning rate. Results In the pH 4.0 phosphate buffer solution, the oxidation peak current and concentration of butylated hydroxytoluene had a good linear relationship in the range of 2.0×10~(-7)-4.0×10~(-5) mol/L. The linear equation was ipa=1.27×10~(-7)+0.23 c, the correlation coefficient(r) was 0.9962, and the limit of detection was 4.0×10~(-8) mol/L. Conclusion This method is simple, rapid and sensitive, which is suitable for the determination of butylated hydroxytoluene content in food.
引文
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[13]胡晴晴,朱庆仁,孙登明.聚L-精氨酸/石墨烯修饰电极对日落黄的测定[J].化学传感器,2016,(2):57-61.Hu QQ,Zhu RQ,Sun DM.The determination of sunset yellow by poly-L-arginine-graphene modified electrode[J].Chem Sens,2016,(2):57-61.
[14]Yu ZY,Li XC,Wang XL,et al.Voltammetric determination of dopamine and norepinphrine on a glassy carbon electrode modified with poly(L-aspartic acid)[J].J Chem Sci,2012,24(2):537-544.
[15]马心英,陈美凤.氧氟沙星的聚缬氨酸修饰电极法测定[J].中国医药工业杂志,2014,45(7):680-683.Ma XY,Chen MF.Electrochemical determination of loxacin by polyvaline modified electrode[J].Chin J Pharm,2014,45(7):680-683.
[16]孙登明,胡文娜,马伟.银掺杂聚L-缬氨酸修饰电极的制备及对肾上腺素的测定[J].分析试验室,2011,30(9):50-54.Sun DM,Hu WN,Ma W.Preparation of a silver doped poly(L-valine)modified electrode and its determination for epinephrine[J].Chin J Anal Lab,2011,30(9):50-54.
[2]李井涛,孙迪,刘洋.气相色谱法测定食用油中BHA和BHT残留量的对比研究[J].食品安全质量检测学报,2018,9(1):173-177.Li JT,Sun D,Liu Y.Comparative study on the determination of residues of BHA and BHT in edible oil by gas chromatography[J].J Food Saf Qual,2018,9(1):173-177.
[3]冷鹏,李亲凯,刘素.基于纳米材料增效的2,6-二叔丁基对甲酚分子印迹电化学传感器的研究及应用[J].化学分析计量,2015,24(4):11-15.Leng P,Li QK,Liu S.Study and application of molecularly imprinted electrochemical sensor for detection of 2,6-di-tert-butyl-4-methylphenol based on nano-materials[J].Chem Anal Meter,2015,24(4):11-15.
[4]邓鹏,巩东营,李桂龙,等.BHT的电化学性质研究[J].中国食物与营养,2013,19(10):29-31.Deng P,Gong DY,Li GL,et al.Study on electrochemistry nature of BHT[J].Food Nutr China,2013,19(10):29-31.
[5]耿红超,韩锦伟,曹晶,等.气质联用法测定液晶材料中微量2,6-二叔丁基对甲酚杂质[J].安徽化工,2017,43(2):120-121.Geng HC,Han JW,Cao J,et al.Determination of BHT in liquid crystal materials by GC-MS method[J].Anhui Chem Ind,2017,43(2):120-121.
[6]佘琼虹,洪瑞申.气质联用法测定植物油中BHA和BHT[J].广东化工,2013,21(4):234-237.She QH,Hong RS.The determination of BHA and BHT in the vegetable oil by GC-MS[J].Guangzhou Chem Ind,2013,21(4):234-237.
[7]余涛,叶坚.高效液相色谱同时测定食品中BHA、BHT、TBHQ方法的研究[J].中国卫生检验杂志,2007,(12):2136-2137.Yu T,Ye J.Determination of BHA,BHT and TBHQ in foods by high performance liquid chromatography[J].Chin J Health Lab Technol,2007,(12):2136-2137.
[8]林太凤,刘阳,王慧琴,等.PSE-HPLC法测定食品中叔丁基羟基茴香醚和2,6-二叔丁基羟基甲苯[J].食品科学,2010,31(14):254-257.Lin TF,Liu Y,Wang HQ,et al.Pressurized solvent extraction and HPLCdetermination of BHA and BHT in food[J].Food Sci,2010,31(14):254-257.
[9]王浩,杨悠悠,刘佟,等.分散式固相萃取结合高效液相色谱法快速测定油脂中三种抗氧化剂含量[J].食品安全质量检测学报,2014,42(11):3620-3625.Wang H,Yang YY,Liu T,et al.Determination of 3 antioxidant residues in edible oil by high performance liquid chromatography with dispersive solid phase extraction[J].J Food Saf Qual,2014,42(11):3620-3625.
[10]王佳蕊,郝雅茹,李书国.电分析法同时快速测定植物油中TBHQ、BHA和BHT的研究[J].中国粮油学报,2018,33(8):126-132.Wang JR,Hao YR,Li SG.Rapid determination of three synthetic antioxidants:TBHQ,BHA and BHT in vegetable oils by electrical method[J].J Chin Cere Oil Assoc,2018,33(8):126-132.
[11]陶琳丽,黄伟,杨秀娟,等.20种氨基酸近红外光谱及其分子结构的相关性[J].光谱学与光谱分析,2016,(9):2766-2772.Tao LL,Huang W,Yang XJ,et al.Correlations between near infrared spectra and molecular structures of 20 standard amino acids[J].Spectrosc Spect Anal,2016,36(9):2766-2772.
[12]孙章华,陈美凤,尹明静,等.聚甘氨酸修饰电极测定食品中的诱惑红[J].食品与发酵工业,2014,(12):185-189.Sun ZH,Chen MF,Yin MJ,et al.Poly(glycine)modified electrode for determination of allura red in food[J].Food Ferm Ind,2014,(12):185-189.
[13]胡晴晴,朱庆仁,孙登明.聚L-精氨酸/石墨烯修饰电极对日落黄的测定[J].化学传感器,2016,(2):57-61.Hu QQ,Zhu RQ,Sun DM.The determination of sunset yellow by poly-L-arginine-graphene modified electrode[J].Chem Sens,2016,(2):57-61.
[14]Yu ZY,Li XC,Wang XL,et al.Voltammetric determination of dopamine and norepinphrine on a glassy carbon electrode modified with poly(L-aspartic acid)[J].J Chem Sci,2012,24(2):537-544.
[15]马心英,陈美凤.氧氟沙星的聚缬氨酸修饰电极法测定[J].中国医药工业杂志,2014,45(7):680-683.Ma XY,Chen MF.Electrochemical determination of loxacin by polyvaline modified electrode[J].Chin J Pharm,2014,45(7):680-683.
[16]孙登明,胡文娜,马伟.银掺杂聚L-缬氨酸修饰电极的制备及对肾上腺素的测定[J].分析试验室,2011,30(9):50-54.Sun DM,Hu WN,Ma W.Preparation of a silver doped poly(L-valine)modified electrode and its determination for epinephrine[J].Chin J Anal Lab,2011,30(9):50-54.