钙螯合胶原多肽与雌激素对去卵巢大鼠骨质改善作用的比较
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摘要
目的将钙螯合胶原多肽(CPCC)的骨质改善效果与雌激素进行比较,为安全的骨质疏松(osteoporosis,OP)防治药物的研发奠定基础。方法切除大鼠双侧卵巢,设置切卵巢组(OVX)、假手术组(sham)、17β-雌二醇(OVX+E_2)注射组与CPCC灌胃组(OVX+CPCC),9周后比较各组的骨骼指标和血液生化指标。结果 OVX组股骨密度显著低于sham组(P<0.01),说明OP发生。和激素一样,CPCC可有效抑制所测指标的异常变化,并使其维持在sham组水平(P>0.05)。然而在抑制体重增加方面,E_2组在第8、9周体重显著低于sham组(P<0.01);在阻止骨矿和骨有机质流失方面,E_2组的Mg、Ca水平显著低于CPCC的中、高剂量组,Cu水平与sham组无差异,而CPCC(中、低剂量)则显著高于sham组。E_2组的Mn、Zn、及羟脯氨酸水平显著低于sham组,而CPCC则可维持在sham组水平。对血液中BGP和Str ACP水平升高的抑制作用,E_2组与OVX组相比差异无显著性,CPCC组则可显著低于OVX组。抑制血钙水平下降时,E_2组与OVX组相比差异无显著性,而CPCC组显著高于OVX组。结论在改善去卵巢大鼠骨质方面,钙螯合胶原多肽比雌激素更为有效。
Objective To compare the ameliorating effect of collagen peptide chelated calcium( CPCC) and estrogen on the bone quality in ovariectomized rats in order to serve the development of safe drugs for prevention of osteoporosis( OP). Methods Bilateral ovariectomized rats were divided into ovariectomized group( OVX),sham group,17β-estradiol injection group( OVX + E_2) and CPCC gavage group( OVX + CCCP). Bone and serum indices of these groups were assessed and compared at 9 weeks after treatment. Results Bone density of the OVX group was significantly lower than the sham group( P < 0. 01),indicating that the rat OP model was successfully established. Like estrogen,CPCC inhibited the abnormal changes of all indices and maintain similar levels with those of the sham group( P > 0. 05),while the body weight gain of the E_2 group at weeks 8 and 9 was significantly lower than those of the sham group( P < 0. 01). As regarding the prevention of bone loss,the Mg and Ca levels of the E_2 group were significantly lower than those of the moderate and high dose CPCC groups. The Cu level was not significantly different compared with the sham group,while those in the moderate and low dose CPCC groups were significantly higher than the sham group. The Mn,Zn and hydroxyproline levels ofthe E_2 group were significantly lower than those of the sham group,while the CPCC group maintained levels similar to that of the sham group. In regarding to the inhibiting effect on the increased blood BGP and StrA CP,the E_2 group was still maintained at levels similar to that of the OVX group,while those of the CPCC group were significantly lower than the OVX group. As regarding the decreased blood Ca,the E_2 group was not significantly different with that of the OVX group,while that of the CPCC group was significantly higher than the OVX group. Conclusions CPCC is more effective than estrogen in ameliorating the bone quality of ovariectomized rats.
Objective To compare the ameliorating effect of collagen peptide chelated calcium( CPCC) and estrogen on the bone quality in ovariectomized rats in order to serve the development of safe drugs for prevention of osteoporosis( OP). Methods Bilateral ovariectomized rats were divided into ovariectomized group( OVX),sham group,17β-estradiol injection group( OVX + E_2) and CPCC gavage group( OVX + CCCP). Bone and serum indices of these groups were assessed and compared at 9 weeks after treatment. Results Bone density of the OVX group was significantly lower than the sham group( P < 0. 01),indicating that the rat OP model was successfully established. Like estrogen,CPCC inhibited the abnormal changes of all indices and maintain similar levels with those of the sham group( P > 0. 05),while the body weight gain of the E_2 group at weeks 8 and 9 was significantly lower than those of the sham group( P < 0. 01). As regarding the prevention of bone loss,the Mg and Ca levels of the E_2 group were significantly lower than those of the moderate and high dose CPCC groups. The Cu level was not significantly different compared with the sham group,while those in the moderate and low dose CPCC groups were significantly higher than the sham group. The Mn,Zn and hydroxyproline levels ofthe E_2 group were significantly lower than those of the sham group,while the CPCC group maintained levels similar to that of the sham group. In regarding to the inhibiting effect on the increased blood BGP and StrA CP,the E_2 group was still maintained at levels similar to that of the OVX group,while those of the CPCC group were significantly lower than the OVX group. As regarding the decreased blood Ca,the E_2 group was not significantly different with that of the OVX group,while that of the CPCC group was significantly higher than the OVX group. Conclusions CPCC is more effective than estrogen in ameliorating the bone quality of ovariectomized rats.
引文
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[9]韩天雨,卜淑敏.去卵巢大鼠血清和骨髓细胞碱性磷酸酶水平变化的动态观察[J].中国实验动物学报,2014,22(4):37-40.
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[13]Jabbar S,Drury J,Fordham JN,et al.Osteoprotegerin,RANKL and bone turnover in postmenopausal osteoporosis[J].J Clin Pathol,2011,64:354-357.
[14]Looker AC,Orwoll ES,Johnston Jr CC,et al.Prevalence of low femoral bone density in older U.S.adults from NHANES III[J].J Bone Miner Res,1997,12:1761-1768.
[15]戴哲浩.糖皮质激素、降钙素对雌性去卵巢大鼠骨强度影响的实验研究[D].湖南:中南大学,2010.
[16]刘昊,张岩,张国彬,等.辛伐他汀不同干预方案对骨质疏松大鼠骨质量的影响[J].中国比较医学杂志,2016,26(6):43-47.
[17]于志锋,顾淑珠,高建军,等.去势大鼠血清ALP动态及骨髓细胞骨形成相关基因表达研究[J].中国骨质疏松杂志,2004,10(2):152-154.
[18]郭盖,卜淑敏,韩天雨.不同干预方法对去卵巢骨质疏松大鼠子宫GSK-3β蛋白表达的影响[J].中国实验动物学报,2014,22(5):53-58.
[2]王运林,匡德宣,马朝霞,等.去卵巢法骨质疏松树鼩模型的建立[J].中国实验动物学报,2015,23(6):562-566.
[3]Nishida A,Ito M,Uetani M,et al.Effects of etidronate on three-dimensional trabecular structure in ovariectomized or sciatic neuretomized rats[J].J Bone Miner Metab.2004,22:335-340.
[4]Rossouw J,Anderson G,Prentice R,et al.Risks and benefits of estrogen plus progestin in healthy postmenopausal women:principal results from the Women’s Health Initiative randomized controlled trial[J].JAMA,2002,288(3):321-333.
[5]Nicklas TA.Calcium intake trends and health consequences from childhood through adulthood[J].J Am Coll Nutrit,2003,22(5):340-356.
[6]田惠光,张兵.保健食品实用指南[M].北京:化学工业出版社,2002,19-25.
[7]霍乃蕊,马俪珍,忻欣,等.SBEF-Ca对骨质疏松大鼠骨密度和骨代谢生化指标的影响[J],中国实验动物学报,2010,18(3):216-220.
[8]霍乃蕊,马俪珍,甄润英,沈钧,张子悦.羊骨胶原肽对去卵巢大鼠骨质疏松的防治作用[J].营养学报,2009,31(3):234-237.
[9]韩天雨,卜淑敏.去卵巢大鼠血清和骨髓细胞碱性磷酸酶水平变化的动态观察[J].中国实验动物学报,2014,22(4):37-40.
[10]曾芳馨,熊中云.抗酒石酸酸性磷酸酶与骨代谢[J].华西医学,2005,20(3):545.
[11]张杰.骨素精粉的制备及其补钙效果的研究[D].吉林:吉林大学,2011.
[12]Ilich JZ,Kerstetter JE.Nutrition in bone health revisited:A story beyond calcium[J].J Am Coll Nutrit.2000,19(6):715-737.
[13]Jabbar S,Drury J,Fordham JN,et al.Osteoprotegerin,RANKL and bone turnover in postmenopausal osteoporosis[J].J Clin Pathol,2011,64:354-357.
[14]Looker AC,Orwoll ES,Johnston Jr CC,et al.Prevalence of low femoral bone density in older U.S.adults from NHANES III[J].J Bone Miner Res,1997,12:1761-1768.
[15]戴哲浩.糖皮质激素、降钙素对雌性去卵巢大鼠骨强度影响的实验研究[D].湖南:中南大学,2010.
[16]刘昊,张岩,张国彬,等.辛伐他汀不同干预方案对骨质疏松大鼠骨质量的影响[J].中国比较医学杂志,2016,26(6):43-47.
[17]于志锋,顾淑珠,高建军,等.去势大鼠血清ALP动态及骨髓细胞骨形成相关基因表达研究[J].中国骨质疏松杂志,2004,10(2):152-154.
[18]郭盖,卜淑敏,韩天雨.不同干预方法对去卵巢骨质疏松大鼠子宫GSK-3β蛋白表达的影响[J].中国实验动物学报,2014,22(5):53-58.