美花石斛HPLC特征图谱研究及其与水煎液相关性分析
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摘要
目的建立美花石斛HPLC特征图谱分析方法,研究美花石斛及其水煎液指纹图谱的相关性。方法采用Kromasil 100-5 C_(18)色谱柱;流动相为乙腈-0.2%磷酸溶液,梯度洗脱;检测波长为280 nm;柱温为35℃;流速为0.8 mL·min~(-1)。结果 16批美花石斛药材样品,标示出22个特征共有峰,相似度为0.854~0.969;10批美花石斛水煎液样品,标示出药材中的16个共有峰,相似度均在0.909以上。经对照品保留时间定位及特征峰紫外光谱分析,鉴别了2个特征峰(峰14为柚皮素、峰16为石斛酚)。每批药材与其水煎液间的相似度为0.815~0.910。结论方法准确可靠稳定,重复性好,为美花石斛质量控制和物质基础研究提供参考。
Objective To establish the method of HPLC fingerprinting analysis on Dendrobium Loddigesii,and study the correlation of HPLC fingerprint between samples prepared from decoction pieces and decoction, respectively.Methods HPLC was performed on Kromasil 100-5-C_(18) column,the acetonitrile-0.2 % phosphoric acid solution was employed as a mobile phase by gradient elution, and detection wavelength was 280 nm; column temperature was 35℃,and flow rate was 0.8 mL·min~(-1). Results Twenty-two common peaks were separated in the samples prepared from decoction pieces of Dendrobium Loddigesii,the similarity of 16 samples was 0.854~0.969. Sixteen common peaks were separated in the samples prepared from decoction of Dendrobium Loddigesii,the similarity of 10 samples were all above 0.909. Two specific peaks were identified through the retention time and ultraviolet spectra of reference substances(peak 14-Naringenin,peak 16-Kaempferol). The similarity between HPLC fingerprints of the decoction piece and decoction was 0.815~0.910. Conclusion The method established in the current study is accurate,stable and reliable,which will provide references for quality control and material base study of Dendrobium Loddigesii.
Objective To establish the method of HPLC fingerprinting analysis on Dendrobium Loddigesii,and study the correlation of HPLC fingerprint between samples prepared from decoction pieces and decoction, respectively.Methods HPLC was performed on Kromasil 100-5-C_(18) column,the acetonitrile-0.2 % phosphoric acid solution was employed as a mobile phase by gradient elution, and detection wavelength was 280 nm; column temperature was 35℃,and flow rate was 0.8 mL·min~(-1). Results Twenty-two common peaks were separated in the samples prepared from decoction pieces of Dendrobium Loddigesii,the similarity of 16 samples was 0.854~0.969. Sixteen common peaks were separated in the samples prepared from decoction of Dendrobium Loddigesii,the similarity of 10 samples were all above 0.909. Two specific peaks were identified through the retention time and ultraviolet spectra of reference substances(peak 14-Naringenin,peak 16-Kaempferol). The similarity between HPLC fingerprints of the decoction piece and decoction was 0.815~0.910. Conclusion The method established in the current study is accurate,stable and reliable,which will provide references for quality control and material base study of Dendrobium Loddigesii.
引文
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[2]冯耀南,刘明,刘俭,等.中药材商品规格质量鉴别[M].广州:暨南大学出版社,1995:193-196.
[3]李满飞,平田正义,徐国钧,等.粉花石斛化学成分的研究[J].药学学报,1991,26(4):307-310.
[4]秦海林,张建新,王峥涛,等.环草石斛的1H-NMR指纹谱图解析.中国中药杂志,2002,27(12):919-923.
[5]刘建华,高玉琼,霍昕,等.金钗石斛、环草石斛挥发性成分研究[J].中成药,2006,28(9):1339-1342.
[6]MASANOBU I T O,MATSUZAKI K,WANG J,et al.Chem Inform abstract new phenanthrenes and stilbenes from dendrobium loddigesii[J].Chem Pharm Bull,2010,58(5):628-633.
[7]李春燕,陆羽,陈烨,等.环草石斛化学成分研究[J].中山大学学报,2013,52(3):73-76.
[8]滕建北.铁皮石斛与美花石斛茎尖生长机理及其多糖累积规律研究[D].成都:成都中医药大学,2009.
[9]梁洁,腾建北,劳文然.不同栽培方法和生长时间的美花石斛中多糖含量的比较[J].华西药学杂志,2010,25(1):63-65.
[10]卢文芸,张宇斌,罗迎春,等.环草石斛愈伤组织和拟原球茎中生物碱和多糖含量的研究[J].种子,2010,29(3):33-35.
[11]陈化,陈竹,梁斌,等.紫外-可见分光光度法测定环草石斛中石斛多糖的含量[J].中国药房,2011,22(39):3690-3692.
[12]朱华,腾建北,蔡毅,等.美花石斛茎的淀粉粒数量、多糖含量与生物碱含量的相关性研究[J].中国中药杂志,2011,36(23):3262-3264.