旱季美花石斛和金沙江石斛形态及生理特性的差异研究
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摘要
本试验以美花石斛和金沙江石斛为材料,采用形态观察和生理指标检测法,研究旱季两种石斛根形态和生理特性方面的差异。结果表明:金沙江石斛根部形成菌根结构,美花石斛根部未发现菌根结构。短时间干旱胁迫下,金沙江石斛的叶绿素a和总叶绿素含量显著高于美花石斛,但叶绿素b含量无显著差异;两种石斛的PSⅡ最大光化学效率无显著差异;金沙江石斛叶片丙二醛含量显著高于美花石斛。综合分析得到:金沙江石斛可能通过形成菌根结构来适应干旱环境,美花石斛的耐旱性可能通过自身调节生理生化反应来完成。
Dendrobium loddigesii and Dendrobium wangliangii grown in dry season were used as experimental materials to analyze their differences in morphological and physiological characteristics. The results showed that the mycorrhizal structure was observed in the roots of Dendrobium wangliangii, but was not found in the roots of Dendrobium loddigesii. Under short-term drought stress, the chlorophyll a content and the total chlorophyll content of Dendrobium wangliangii were significantly higher than those of Dendrobium loddigesii, while there was no significant difference in chlorophyll b between them. There was no significant difference in the maximum photochemical efficiency(Fv/Fm) of PSⅡ between the two species of Dendrobium. The malondialdehyde(MDA) content in the leaves of Dendrobium wangliangii was significantly higher than that of Dendrobium loddigesii. In conclusion, Dendrobium wangliangii might adapt to drought by forming mycorrhizal structure, and the drought tolerance of Dendrobium loddigesii might be accomplished by self-regulating physiological and biochemical responses.
Dendrobium loddigesii and Dendrobium wangliangii grown in dry season were used as experimental materials to analyze their differences in morphological and physiological characteristics. The results showed that the mycorrhizal structure was observed in the roots of Dendrobium wangliangii, but was not found in the roots of Dendrobium loddigesii. Under short-term drought stress, the chlorophyll a content and the total chlorophyll content of Dendrobium wangliangii were significantly higher than those of Dendrobium loddigesii, while there was no significant difference in chlorophyll b between them. There was no significant difference in the maximum photochemical efficiency(Fv/Fm) of PSⅡ between the two species of Dendrobium. The malondialdehyde(MDA) content in the leaves of Dendrobium wangliangii was significantly higher than that of Dendrobium loddigesii. In conclusion, Dendrobium wangliangii might adapt to drought by forming mycorrhizal structure, and the drought tolerance of Dendrobium loddigesii might be accomplished by self-regulating physiological and biochemical responses.
引文
[1] Szechyńska-Hebda M, Czamocka W, Hebda M,et al . PAD4, LSD1 and EDS1 regulate drought tolerance, plant biomass production, and cell wall properties[J]. Plant Cell Reports, 2016,35(3):527-539.
[2] Zou Y N, Huang Y M, Wu Q S, et al.Mycorrhiza-induced lower oxidative burst is related with higher antioxidant enzyme activities, net H2O2 effluxes,and Ca2+ influxes in trifoliate orange roots under drought stress[J]. Mycorrhiza, 2015,25(2):143-152.
[3] Li S F, Fan C M, Li Y, et al. Effects of drought and salt-stresses on gene expression in Caragana korshinskii seedlings revealed by RNA-seq[J]. BMC Genomics, 2016,17:200.
[4] 吴雪俊,安静.丛枝菌根提高百喜草抗旱性的机制研究[J].安徽师范大学学报(自然科学版),2014,37(1):47-52.
[5] Hu G W, Long C L, Jin X H. Dendrobium wangliangii(Orchidaceae), a new species belonging to section Dendrobium from Yunnan, China[J]. Botanical Journal of the Linnean Society, 2008, 157:217-221.
[6] 韦艳梅,谭小明,周雅琴. 不同因素对濒危药用植物美花石斛壮苗的影响[J]. 基因组学与应用生物学,2015, 34(12): 2743-2747.
[7] Madhava Rao K V,Sresty T V S.Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan(L.)Millspaugh)in response to Zn and Ni stresses[J].Plant Science, 2000, 157(1):113-128.
[8] 李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社,2000.
[9] 陈连庆,王小明,裴致达. 石斛气生的兰科菌根组织结构及其对御旱研究[J]. 生态环境学报,2010,19(1):160-164.
[10] 白淑兰,王琚钢,峥嵘,等. 外生菌根对干旱胁迫的响应[J]. 全国菌根学术研讨会,2011,31(6):1571-1576.
[11] 刘方炎,李昆,孙永玉,等. 横断山区干热河谷气候及其对植被恢复的影响[J]. 长江流域资源与环境,2010,19(12): 1386-1391.
[12] 腾开琼,肖琳琳,刘诗慧,等. 短时干旱胁迫对水旱稻叶绿素荧光的影响[J]. 河南农业,2018(4):59-60.
[13] 吴刘萍,许衍才,廖飞雄,等. 杜鹃红山茶叶片叶绿素荧光对干旱胁迫及复水处理的响应[J]. 广东农业科学,2017,44(10):25-31.
[14] 苏畅,李枝林,任智慧,等. 辐射诱发叶艺兰形态特征和生理特性差异的研究[J]. 西部林业科学,2016,45(3):38-43.
[15] 何文铸,张彪,王培,等. 利用叶绿素含量及荧光动力学参数评价青贮玉米耐旱关键指标研究[J]. 干旱地区农业研究,2013,31(3):31-38.
[16] 马坤,王彦淇,杨建军,等.不同干旱胁迫条件下丛枝菌根真菌对木棉叶绿素荧光参数的影响[J]. 植物资源与环境学报,2017,26(3):35-43.
[17] 李婉娇,廖里平,高永,等. 植物延缓剂对羊柴抗旱生理特性的调控效应[J]. 安徽农业大学学报,2018, 45(1):166-170.
[2] Zou Y N, Huang Y M, Wu Q S, et al.Mycorrhiza-induced lower oxidative burst is related with higher antioxidant enzyme activities, net H2O2 effluxes,and Ca2+ influxes in trifoliate orange roots under drought stress[J]. Mycorrhiza, 2015,25(2):143-152.
[3] Li S F, Fan C M, Li Y, et al. Effects of drought and salt-stresses on gene expression in Caragana korshinskii seedlings revealed by RNA-seq[J]. BMC Genomics, 2016,17:200.
[4] 吴雪俊,安静.丛枝菌根提高百喜草抗旱性的机制研究[J].安徽师范大学学报(自然科学版),2014,37(1):47-52.
[5] Hu G W, Long C L, Jin X H. Dendrobium wangliangii(Orchidaceae), a new species belonging to section Dendrobium from Yunnan, China[J]. Botanical Journal of the Linnean Society, 2008, 157:217-221.
[6] 韦艳梅,谭小明,周雅琴. 不同因素对濒危药用植物美花石斛壮苗的影响[J]. 基因组学与应用生物学,2015, 34(12): 2743-2747.
[7] Madhava Rao K V,Sresty T V S.Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan(L.)Millspaugh)in response to Zn and Ni stresses[J].Plant Science, 2000, 157(1):113-128.
[8] 李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社,2000.
[9] 陈连庆,王小明,裴致达. 石斛气生的兰科菌根组织结构及其对御旱研究[J]. 生态环境学报,2010,19(1):160-164.
[10] 白淑兰,王琚钢,峥嵘,等. 外生菌根对干旱胁迫的响应[J]. 全国菌根学术研讨会,2011,31(6):1571-1576.
[11] 刘方炎,李昆,孙永玉,等. 横断山区干热河谷气候及其对植被恢复的影响[J]. 长江流域资源与环境,2010,19(12): 1386-1391.
[12] 腾开琼,肖琳琳,刘诗慧,等. 短时干旱胁迫对水旱稻叶绿素荧光的影响[J]. 河南农业,2018(4):59-60.
[13] 吴刘萍,许衍才,廖飞雄,等. 杜鹃红山茶叶片叶绿素荧光对干旱胁迫及复水处理的响应[J]. 广东农业科学,2017,44(10):25-31.
[14] 苏畅,李枝林,任智慧,等. 辐射诱发叶艺兰形态特征和生理特性差异的研究[J]. 西部林业科学,2016,45(3):38-43.
[15] 何文铸,张彪,王培,等. 利用叶绿素含量及荧光动力学参数评价青贮玉米耐旱关键指标研究[J]. 干旱地区农业研究,2013,31(3):31-38.
[16] 马坤,王彦淇,杨建军,等.不同干旱胁迫条件下丛枝菌根真菌对木棉叶绿素荧光参数的影响[J]. 植物资源与环境学报,2017,26(3):35-43.
[17] 李婉娇,廖里平,高永,等. 植物延缓剂对羊柴抗旱生理特性的调控效应[J]. 安徽农业大学学报,2018, 45(1):166-170.