α-酮戊二酸对L-羟脯氨酸产量的影响
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摘要
微生物合成L-羟脯氨酸时需要α-酮戊二酸的参与,而细胞内α-酮戊二酸的含量有限,限制了L-羟脯氨酸的高效合成。因此该实验通过在L-羟脯氨酸发酵过程中外源添加α-酮戊二酸,来考察α-酮戊二酸对发酵菌体生长、L-羟脯氨酸产量、糖酸转化率和代谢流的影响。结果表明,α-酮戊二酸对菌体生长有一定的抑制作用,但在一定浓度范围内,外源添加α-酮戊二酸能有效提高L-羟脯氨酸的产量和糖酸转化率,当随糖流加5 g/L(初始发酵液)α-酮戊二酸时,L-羟脯氨酸产量能够达到最大值62.14 g/L,糖酸转化率为22.37%,与不添加α-酮戊二酸相比,分别提高了47.85%和13.04%,同时,L-羟脯氨酸的合成代谢流提高了11.98%,副产物乙酸合成代谢流减少了29.42%。
The synthesis of L-hydroxyproline by microorganisms requires the participation of α-ketoglutarate,and the limited content of α-ketoglutarate in cells limits the efficient synthesis of L-hydroxyproline.Therefore,in this study,α-ketoglutarate was exogenously added during L-hydroxyproline fermentation to study the effect of α-ketoglutarate on cell growth,L-hydroxyproline production,glucose to acid conversion ratio and metabolic flux.The results showed that the cell growth was inhibited by α-ketoglutarate to a certain extent.The production of L-hydroxyproline and the glucose to acid conversion ratio could be effectively increased by the addition of α-ketoglutarate within a certain concentration range.When α-ketoglutarate was added along with glucose 5 g/L(relative to the initial volume of fermentation broth),the highest production of L-hydroxyproline was62.14 g/L,and the glucose to acid conversion ratio was 22.37%.Compared with the fermentation without extra α-ketoglutarate,the L-hydroxyproline production and glucose to acid conversion ratio increased by 47.85% and 13.04%,respectively.At the same time,the metabolic flux of L-hydroxyproline increased by 11.98% and the byproduct acetic acid metabolic flux decreased by 29.42%.
The synthesis of L-hydroxyproline by microorganisms requires the participation of α-ketoglutarate,and the limited content of α-ketoglutarate in cells limits the efficient synthesis of L-hydroxyproline.Therefore,in this study,α-ketoglutarate was exogenously added during L-hydroxyproline fermentation to study the effect of α-ketoglutarate on cell growth,L-hydroxyproline production,glucose to acid conversion ratio and metabolic flux.The results showed that the cell growth was inhibited by α-ketoglutarate to a certain extent.The production of L-hydroxyproline and the glucose to acid conversion ratio could be effectively increased by the addition of α-ketoglutarate within a certain concentration range.When α-ketoglutarate was added along with glucose 5 g/L(relative to the initial volume of fermentation broth),the highest production of L-hydroxyproline was62.14 g/L,and the glucose to acid conversion ratio was 22.37%.Compared with the fermentation without extra α-ketoglutarate,the L-hydroxyproline production and glucose to acid conversion ratio increased by 47.85% and 13.04%,respectively.At the same time,the metabolic flux of L-hydroxyproline increased by 11.98% and the byproduct acetic acid metabolic flux decreased by 29.42%.
引文
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[11]PHANG J M,DONALD S P,PANDHARE J,et al.The metabolism of proline,a stress substrate,modulates carcinogenic pathways[J].Amino Acid,2008,35(4):681-690.
[12]AOKI M,SUTO K,KOMATSU M,et al.Increasing effect of an oral intake of L-hydroxyproline on the soluble collagen content of skin and collagen fragments in rat serum[J].J Agr Chem Soc Jpn,2012,76(6):1242-1244.
[13]左瑞雅,周小华,杜首英.L-羟脯氨酸-Zn(Ⅱ)的配合机制及其抗氧化性研究[J].生物工程学报,2007,23(4):704-709.
[14]张自强,赵东旭,杨新林.羟脯氨酸的研究与开发[J].氨基酸和生物资源,2006(1):55-58,62.
[15]张胜利.产反式-4-羟基-L-脯氨酸大肠杆菌菌株的改造及发酵条件初步研究[D].无锡:江南大学,2015.
[16]崔丽.全细胞催化产羟脯氨酸的大肠杆菌的代谢工程研究[D].北京:中国科学院大学,2015.
[17]蔡萌萌,户红通,刘子强,等.活细胞在线监控L-羟脯氨酸补料发酵工艺的研究[J].食品与发酵工业,2018,44(5):10-15.
[2]KUTTAN R,RADHAKRISHNAN A N.Biochemistry of the hydroxyprolines[J].Adv Enzymol Relat Area Mol Biol,1973,37:273.
[3]REMUZON P.Chem Inform abstract:trans-4-hydroxy-L-proline,a useful and versatile chiral starting block[J].Cheminform,1996,52(44):13803-13835.
[4]BRANDS K M,JOBSON R B,CONRAD K M,et al.Efficient One-Pot synthesis of the 2-aminocarbonylpyrrolidin-4-ylthio-containing side chain(I)of the new broad-spectrum carbapenem antibiotic ertapenem[J].Cheminform,2002,67(14):4771-4776.
[5]贾晓波,庞会从.L-羟脯氨酸用于减肥的实验研究[J].氨基酸和生物资源,2003,25(3):60-62.
[6]LEE R,YANG J,LIN T.Novel,biodegradable,functional poly(ester-carbonate)s by copolymerization of trans-4-hydroxy-L-proline with cyclic carbonate bearing a pendent carboxylic group[J].J Polym Sci Part APolym Chem,2004,42(10):2303-2312.
[7]KUMAR A V,RAO K R.Trans-4-hydroxy-L-proline:a novel starting material for N-alkyl pyrroles synthesis[J].Tetrahed Lett,2011,52(25):3237-3239.
[8]REDDY V P,KUMAR A V,RAO K R.Chem Inform abstract:new strategy for the synthesis of N-aryl pyrroles:Cu-catalyzed C-N cross-coupling reaction of trans-4-hydroxy-L-proline with aryl halides[J].Tetrahed Lett,2011,42(21):777-780.
[9]WU G,BAZER F W,BURGHARDT R C,et al.Proline and hydroxyproline metabolism:implications for animal and human nutrition[J].Amino Acids,2011,40(4):1053-1063.
[10]周汉林,莫放,黄鸿威,等.羟脯氨酸在反刍动物营养研究中的应用[J].草业科学,2005,22(11):84-87.
[11]PHANG J M,DONALD S P,PANDHARE J,et al.The metabolism of proline,a stress substrate,modulates carcinogenic pathways[J].Amino Acid,2008,35(4):681-690.
[12]AOKI M,SUTO K,KOMATSU M,et al.Increasing effect of an oral intake of L-hydroxyproline on the soluble collagen content of skin and collagen fragments in rat serum[J].J Agr Chem Soc Jpn,2012,76(6):1242-1244.
[13]左瑞雅,周小华,杜首英.L-羟脯氨酸-Zn(Ⅱ)的配合机制及其抗氧化性研究[J].生物工程学报,2007,23(4):704-709.
[14]张自强,赵东旭,杨新林.羟脯氨酸的研究与开发[J].氨基酸和生物资源,2006(1):55-58,62.
[15]张胜利.产反式-4-羟基-L-脯氨酸大肠杆菌菌株的改造及发酵条件初步研究[D].无锡:江南大学,2015.
[16]崔丽.全细胞催化产羟脯氨酸的大肠杆菌的代谢工程研究[D].北京:中国科学院大学,2015.
[17]蔡萌萌,户红通,刘子强,等.活细胞在线监控L-羟脯氨酸补料发酵工艺的研究[J].食品与发酵工业,2018,44(5):10-15.