羊骨胶原肽对去卵巢大鼠的骨质改善作用
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摘要
[目的]探讨羊骨胶原肽(collagen peptide,CP)对去卵巢大鼠的骨质改善作用,为畜骨的高值利用和骨质疏松非药物防治的研发提供试验依据。[方法]切除大鼠双侧卵巢,设置切卵巢组(OVX)、雌激素组(E_2)、羊骨胶原肽的高、中、低剂量组(CP-H、CP-M、CP-L),另设假手术组(SHAM)。各组8只,饲喂基础日粮,CP各剂量组灌胃相应受试物,SHAM组和OVX组灌胃等体积蒸馏水,E_2组注射雌激素。灌胃9 w后处死大鼠,取股骨进行骨密度、骨矿物质、羟脯氨酸含量测定,并测定血清PINP及β-CTx水平。[结果]OVX组骨密度((1.767±0.031) g·cm~(-3))、骨矿物质(Ca、Mg、Zn、Mn)((9.73±0.14) mg·g~(-1),(0.34±0.01) mg·g~(-1),(30.48±1.48)μg·g~(-1),(1.70±0.24)μg·g~(-1))以及羟脯氨酸含量((1.922±0.12)μg·mg~(-1))均显著低于SHAM组((2.283±0.47) g·cm~(-3),(12.59±2.78) mg·g~(-1),(0.49±0.10) mg·g~(-1),(33.78±0.43)μg·g~(-1),(1.94±0.29)μg·g~(-1),(2.258±0.22)μg·mg~(-1)),血液PINP((3 528.37±154.74) pg·mL~(-1))和β-CTx((529.67±23.63) pg·mL~(-1))水平显著高于SHAM组((2.283±0.47) g·cm~(-3),(2 258.55±140.87) pg·mL~(-1),(370.98±61.89) pg·mL~(-1)),说明骨质疏松模型建模成功,CP各剂量组和E_2组骨密度、所测骨矿物质含量、羟脯氨酸含量均显著高于OVX组,血清PINP及β-CTx水平显著低于OVX组(P<0.05)。[结论]羊骨胶原肽可提高去卵巢大鼠股骨密度,抑制骨矿物质和骨有机质的丢失,降低血清骨代谢标志物水平,从而具有骨质改善作用。
[Objective] The positive effect of sheep bone collagen peptide(CP) on bone quality of ovariectomized rats was investigated to provide solid evidence for adding-value utilization of animal bone resources and non-medicine prevention of osteoporosis. [Methods] Bilateral ovariectomized rats were divided into six groups, including ovariectomized(OVX), estrogen(E_2), CP at high level(CP-H), CP at medium level(CP-M), CP at low level(CP-L groups), and SHAM group which were sham operated. Eight rats were involved in each group. In addition to basal diet for all groups, CP groups were also intragastrically administered with CP at different dosages, while SHAM and OVX group were treated with equal-volume distilled water, and rats in E_2 group were injected with 17-βestrodial. After nine weeks treatment, femoral bones of rats in each group were sampled to determine the bone density, bone mineral, and hydroxyproline content, and blood samples were collected to determine serum PINP and β-CTx levels. [Results] Results showed that the bone density, bone minerals(Ca, Mg, Zn, Mn), and hydroxyproline content of OVX group((1.767±0.031) g·cm~(-3),(9.73±0.14) mg·g~(-1),(0.34±0.01) mg·g~(-1),(30.48±1.48) μg·g~(-1),(1.70±0.24) μg·g~(-1), and(1.922±0.12) μg·mg~(-1), respectively) were significantly lower than those of SHAM group((2.283±0.47) g·cm~(-3),(12.59±2.78) mg·g~(-1),(0.49±0.10) mg·g~(-1),(33.78±0.43) μg·g~(-1),(1.94±0.29) μg·g~(-1), and(2.258±0.22) μg·mg~(-1), respectively). The serum PINP and β-CTx levels of OVX at(3528.37±154.74) and(529.67±23.63) pg·mL~(-1) were significantly higher than those in SHAM group at(2258.55±140.87) and(370.98±61.89) pg·mL~(-1)(P<0.05), which indicated that osteoporosis model was successfully established. Bone density, bone mineral, and hydroxyproline content in both CP groups and E_2 group were significantly greater than those in the OVX group, while serum PINP and β-CTx levels were significantly lower than OVX group(P<0.05). [Conclusion] Sheep bone collagen peptide can inhibit the loss of bone mineral and bone organic matter in ovariectomized rats and can be an important role in bone quality improvement.
[Objective] The positive effect of sheep bone collagen peptide(CP) on bone quality of ovariectomized rats was investigated to provide solid evidence for adding-value utilization of animal bone resources and non-medicine prevention of osteoporosis. [Methods] Bilateral ovariectomized rats were divided into six groups, including ovariectomized(OVX), estrogen(E_2), CP at high level(CP-H), CP at medium level(CP-M), CP at low level(CP-L groups), and SHAM group which were sham operated. Eight rats were involved in each group. In addition to basal diet for all groups, CP groups were also intragastrically administered with CP at different dosages, while SHAM and OVX group were treated with equal-volume distilled water, and rats in E_2 group were injected with 17-βestrodial. After nine weeks treatment, femoral bones of rats in each group were sampled to determine the bone density, bone mineral, and hydroxyproline content, and blood samples were collected to determine serum PINP and β-CTx levels. [Results] Results showed that the bone density, bone minerals(Ca, Mg, Zn, Mn), and hydroxyproline content of OVX group((1.767±0.031) g·cm~(-3),(9.73±0.14) mg·g~(-1),(0.34±0.01) mg·g~(-1),(30.48±1.48) μg·g~(-1),(1.70±0.24) μg·g~(-1), and(1.922±0.12) μg·mg~(-1), respectively) were significantly lower than those of SHAM group((2.283±0.47) g·cm~(-3),(12.59±2.78) mg·g~(-1),(0.49±0.10) mg·g~(-1),(33.78±0.43) μg·g~(-1),(1.94±0.29) μg·g~(-1), and(2.258±0.22) μg·mg~(-1), respectively). The serum PINP and β-CTx levels of OVX at(3528.37±154.74) and(529.67±23.63) pg·mL~(-1) were significantly higher than those in SHAM group at(2258.55±140.87) and(370.98±61.89) pg·mL~(-1)(P<0.05), which indicated that osteoporosis model was successfully established. Bone density, bone mineral, and hydroxyproline content in both CP groups and E_2 group were significantly greater than those in the OVX group, while serum PINP and β-CTx levels were significantly lower than OVX group(P<0.05). [Conclusion] Sheep bone collagen peptide can inhibit the loss of bone mineral and bone organic matter in ovariectomized rats and can be an important role in bone quality improvement.
引文
[1]曹丽梅, 李波. 骨质疏松症的药物治疗新进展[J]. 中国药事, 2005, 19(11):681-684.
[2]刘学军. 骨质疏松症[J]. 中国矫形外科杂志, 2006, 14(21):1668-1670.
[3]贾齐新, 孙儒, 任豫黔,等. 老年骨质疏松症的药物治疗新进展[J]. 中国社区医师, 2018(3):6.
[4]杨巍, 成晓瑜, 陈文华,等. 畜禽骨深加工技术与应用现状[J]. 肉类研究, 2009(11):75-79.
[5]蒋智然. 胶原肽的制备及其促进成骨细胞增殖分化活性研究[D].南宁:广西大学, 2014.
[6]姚薇, 甄守艳, 王钰慧,等. 羊骨粉复合蛋白酶水解工艺[J]. 食品工业, 2016(9):8-11.
[7]霍乃蕊, 马俪珍, 甄润英,等. 羊骨胶原肽对去卵巢大鼠骨质疏松症的防治作用[J]. 营养学报, 2009, 31(3):234-237.
[8]张曼华, 王保岚, YING N ZHANG FOUTZ. 骨密度测量技术诊断骨质疏松的评价[J]. 中国骨质疏松杂志, 2007, 13(11):818-820.
[9]顾新丰, 郑昱新, 庞坚,等. 膝关节骨矿物质密度测定研究进展[J]. 国际骨科学杂志, 2014, 35(3):167-169.
[10]蔡若男. 糖基化终产物对成骨细胞Ⅰ型胶原积聚的影响[D]. 南京:东南大学, 2011.
[11]陈玮. 中青年Graves病患者血清PINP及β-CTx与骨代谢关系分析[D]. 合肥:安徽医科大学, 2013.
[12]田茂友, 李洪洋. 去卵巢大鼠骨质疏松模型研究[J]. 现代预防医学, 2007, 34(12):2239-2241.
[13]董文彦, 吴丽萍, 李聪,等. D-半乳糖对小鼠骨矿物质、羟脯氨酸含量及最大载荷的影响[J]. 中国骨质疏松杂志, 2004, 10(2):147-149.
[14]王卫强. 穴位埋线对去卵巢大鼠骨密度及相关细胞因子的影响[J]. 实用中医药杂志, 2017, 33(2):112-113.
[15]郭鱼波,马如风,王丽丽,等.骨质疏松动物模型及其评价方法的研究进展[J].中国骨质疏松杂志,2015,21(9):1149-1154.
[16]陈玮, 徐将, 陈超,等. 中青年甲亢患者血清PINP、β-CTx与骨密度关系分析[J]. 安徽医科大学学报, 2014(6):804-807.
[17]董培智, 曹丽梅, 李波. 去卵巢骨质疏松症大鼠模型骨密度的变化[J]. 动物学杂志, 2004, 39(6):87-90.
[18]蒋宜伟, 刘宗权, 宋敏,等. 淫羊藿不同提取物对去势大鼠PINP、NTx影响的实验研究[J]. 中国骨质疏松杂志, 2014,20(2):142-144.
[19]韩龙,吴水培.注射用骨肽对去卵巢骨质疏松大鼠骨微结构、骨密度和骨生物力学性能的影响[J].中国比较医学杂志,2018,28(6):79-83.
[20]陈丽思, 黄志荣, 吴海游,等. 辅酶Q10对去卵巢大鼠骨质和骨密度的影响[J]. 中国临床药理学与治疗学, 2016, 21(8):868-872.
[21]冯有辉, 许碧连, 何康,等. 影响去卵巢大鼠骨量因素的灰色关联分析[J]. 中国临床药理学与治疗学, 2005, 10(7):747-749.
[22]谢斌, 陈允震, 刘海春. 骨胶原在骨质疏松中的作用[J]. 中国老年学杂志, 2009, 29(16):2057-2059.
[23]那晓琳, 禹萍, 李璐璐. 大豆异黄酮对去卵巢大鼠骨矿物质含量及骨密度的影响[J]. 中国组织工程研究, 2004, 8(15):2890-2891.
[2]刘学军. 骨质疏松症[J]. 中国矫形外科杂志, 2006, 14(21):1668-1670.
[3]贾齐新, 孙儒, 任豫黔,等. 老年骨质疏松症的药物治疗新进展[J]. 中国社区医师, 2018(3):6.
[4]杨巍, 成晓瑜, 陈文华,等. 畜禽骨深加工技术与应用现状[J]. 肉类研究, 2009(11):75-79.
[5]蒋智然. 胶原肽的制备及其促进成骨细胞增殖分化活性研究[D].南宁:广西大学, 2014.
[6]姚薇, 甄守艳, 王钰慧,等. 羊骨粉复合蛋白酶水解工艺[J]. 食品工业, 2016(9):8-11.
[7]霍乃蕊, 马俪珍, 甄润英,等. 羊骨胶原肽对去卵巢大鼠骨质疏松症的防治作用[J]. 营养学报, 2009, 31(3):234-237.
[8]张曼华, 王保岚, YING N ZHANG FOUTZ. 骨密度测量技术诊断骨质疏松的评价[J]. 中国骨质疏松杂志, 2007, 13(11):818-820.
[9]顾新丰, 郑昱新, 庞坚,等. 膝关节骨矿物质密度测定研究进展[J]. 国际骨科学杂志, 2014, 35(3):167-169.
[10]蔡若男. 糖基化终产物对成骨细胞Ⅰ型胶原积聚的影响[D]. 南京:东南大学, 2011.
[11]陈玮. 中青年Graves病患者血清PINP及β-CTx与骨代谢关系分析[D]. 合肥:安徽医科大学, 2013.
[12]田茂友, 李洪洋. 去卵巢大鼠骨质疏松模型研究[J]. 现代预防医学, 2007, 34(12):2239-2241.
[13]董文彦, 吴丽萍, 李聪,等. D-半乳糖对小鼠骨矿物质、羟脯氨酸含量及最大载荷的影响[J]. 中国骨质疏松杂志, 2004, 10(2):147-149.
[14]王卫强. 穴位埋线对去卵巢大鼠骨密度及相关细胞因子的影响[J]. 实用中医药杂志, 2017, 33(2):112-113.
[15]郭鱼波,马如风,王丽丽,等.骨质疏松动物模型及其评价方法的研究进展[J].中国骨质疏松杂志,2015,21(9):1149-1154.
[16]陈玮, 徐将, 陈超,等. 中青年甲亢患者血清PINP、β-CTx与骨密度关系分析[J]. 安徽医科大学学报, 2014(6):804-807.
[17]董培智, 曹丽梅, 李波. 去卵巢骨质疏松症大鼠模型骨密度的变化[J]. 动物学杂志, 2004, 39(6):87-90.
[18]蒋宜伟, 刘宗权, 宋敏,等. 淫羊藿不同提取物对去势大鼠PINP、NTx影响的实验研究[J]. 中国骨质疏松杂志, 2014,20(2):142-144.
[19]韩龙,吴水培.注射用骨肽对去卵巢骨质疏松大鼠骨微结构、骨密度和骨生物力学性能的影响[J].中国比较医学杂志,2018,28(6):79-83.
[20]陈丽思, 黄志荣, 吴海游,等. 辅酶Q10对去卵巢大鼠骨质和骨密度的影响[J]. 中国临床药理学与治疗学, 2016, 21(8):868-872.
[21]冯有辉, 许碧连, 何康,等. 影响去卵巢大鼠骨量因素的灰色关联分析[J]. 中国临床药理学与治疗学, 2005, 10(7):747-749.
[22]谢斌, 陈允震, 刘海春. 骨胶原在骨质疏松中的作用[J]. 中国老年学杂志, 2009, 29(16):2057-2059.
[23]那晓琳, 禹萍, 李璐璐. 大豆异黄酮对去卵巢大鼠骨矿物质含量及骨密度的影响[J]. 中国组织工程研究, 2004, 8(15):2890-2891.