一测多评法比较毛橘红与光橘红5种黄酮类成分含量
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摘要
目的建立一测多评法同时测定毛橘红与光橘红中5种黄酮类成分的含量,并进行分析。方法采用HPLC法,以柚皮苷为内参物,建立其与新橙皮苷、野漆树苷、柚皮素和芹菜素的相对校正因子,并利用相对校正因子计算毛橘红与光橘红样品中各成分含量。同时通过外标法计算各成分含量,并比较2种方法的差异。采用t检验比较分析毛橘红与光橘红中5种黄酮类成分含量。结果柚皮苷与新橙皮苷、野漆树苷、柚皮素、芹菜素的校正因子分别为0.898、1.519、0.313、0.406,RSD<3.0%。一测多评法与外标法计算得到的含量测定结果无显著差异。毛橘红与光橘红中的柚皮苷、野漆树苷、柚皮素含量存在显著差异。结论以柚皮苷为内参物建立的相对校正因子准确、可行,毛橘红与光橘红黄酮类成分存在很大差异,一测多评法可用于毛橘红与光橘红的质量控制。
Objective To establish quantitative analysis of multi-components by single marker(QAMS) to compare five flavones in Citrus grandis "Tomentosa" and C. grandis. Methods Four relative correction factors(RCFs) of neohesperidin, rhoifolin, naringenin, apigenin were established in the HPLC method with the Naringin as internal standard, which were used to determine the content of four flavones in C. grandis "Tomentosa" and C. grandis. Meanwhile, external standard method(ESM) was employed to calculate the content of five flavones. The difference between ESM and QAMS were analyzed to evaluate the accuracy of QAMS. T-test was used to compare five flavones in C. grandis ‘Tomentosa' and C. grandis. Results RCFs of neohesperidin, rhoifolin, naringenin, apigenin were 0.898, 1.519, 0.313, 0.406, and repeatability was good in different experimental conditions(RSD < 3.0 %). There were no significant differences in the quantitative analysis results of two methods. Content of naringin, rhoifolin and naringenin in two species were significant different. Conclusion The RCFs of neohesperidin, rhoifolin, naringenin, apigenin as reference to naringin are accurate and feasible. Content of flavones in Citrus grandis "Tomentosa" and C. grandis are obvious different. QASM can be applied as a strategy for quality control of C. grandis.
Objective To establish quantitative analysis of multi-components by single marker(QAMS) to compare five flavones in Citrus grandis "Tomentosa" and C. grandis. Methods Four relative correction factors(RCFs) of neohesperidin, rhoifolin, naringenin, apigenin were established in the HPLC method with the Naringin as internal standard, which were used to determine the content of four flavones in C. grandis "Tomentosa" and C. grandis. Meanwhile, external standard method(ESM) was employed to calculate the content of five flavones. The difference between ESM and QAMS were analyzed to evaluate the accuracy of QAMS. T-test was used to compare five flavones in C. grandis ‘Tomentosa' and C. grandis. Results RCFs of neohesperidin, rhoifolin, naringenin, apigenin were 0.898, 1.519, 0.313, 0.406, and repeatability was good in different experimental conditions(RSD < 3.0 %). There were no significant differences in the quantitative analysis results of two methods. Content of naringin, rhoifolin and naringenin in two species were significant different. Conclusion The RCFs of neohesperidin, rhoifolin, naringenin, apigenin as reference to naringin are accurate and feasible. Content of flavones in Citrus grandis "Tomentosa" and C. grandis are obvious different. QASM can be applied as a strategy for quality control of C. grandis.
引文
[1]中国药典[S].一部.2015.
[2]林励,陈志霞,袁旭江,等.两种化橘红的质量鉴别[J].广州中医药大学学报,2004,21(4):308-312.
[3]Gao S,Li P B,Yang H L,et al.Antitussive effect of naringin on experimentally induced cough in guinea pigs[J].Planta Med,2010,77(1):16-21.
[4]陈南迪,方妙玉,于超凡,等.毛橘红总黄酮指纹图谱与其抗氧化活性的谱效关系研究[J].广州中医药大学学报,2012,29(6):702-706.
[5]林建云,易延逵,蔡光先,等.中药多指标质量控制体系的研究分析[J].辽宁中医杂志,2013,40(8):1674-1676.
[6]刘晓涵,陈永刚,林励,等.化橘红中柚皮苷和野漆树苷含量同时测定方法的建立[J].中药新药与临床药理,2010,12(6):640-642.
[7]邓少东,王莲婧,林励,等.UPLC法测定酸水解前后化橘红中4种黄酮类成分的含量[J].中华中医药杂志,2012,27(4):924-928.
[8]李吉华.化橘红中柚皮苷、柚皮芸香苷、野漆树苷和柚皮素的HPLC测定[J].山东中医杂志,2012,31(4):277-278.
[9]陆兔林,石上梅,蔡宝昌,等.基于一测多评的中药多成分定量研究进展[J].中草药,2012,43(12):2525-2529.
[10]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-658.
[11]袁旭江,李春阳,张平.一测多评法测定鸡骨草叶中3种黄酮碳苷含量[J].中药新药与临床药理,2014,25(4):493-518.
[12]万青,涂楚月,熊慧,等.一测多评法测定藏药金腰草中4种黄酮类化学成分的含量[J].药物分析杂志,2016,36(6):1053-1058.
[13]吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类成分[J].中草药,2017,48(4):777-781.
[14]林励,李向明,万建义,等.化橘红药材质量评价、监测与应用研究[J].中国现代中药,2010,12(8):21-26.
[2]林励,陈志霞,袁旭江,等.两种化橘红的质量鉴别[J].广州中医药大学学报,2004,21(4):308-312.
[3]Gao S,Li P B,Yang H L,et al.Antitussive effect of naringin on experimentally induced cough in guinea pigs[J].Planta Med,2010,77(1):16-21.
[4]陈南迪,方妙玉,于超凡,等.毛橘红总黄酮指纹图谱与其抗氧化活性的谱效关系研究[J].广州中医药大学学报,2012,29(6):702-706.
[5]林建云,易延逵,蔡光先,等.中药多指标质量控制体系的研究分析[J].辽宁中医杂志,2013,40(8):1674-1676.
[6]刘晓涵,陈永刚,林励,等.化橘红中柚皮苷和野漆树苷含量同时测定方法的建立[J].中药新药与临床药理,2010,12(6):640-642.
[7]邓少东,王莲婧,林励,等.UPLC法测定酸水解前后化橘红中4种黄酮类成分的含量[J].中华中医药杂志,2012,27(4):924-928.
[8]李吉华.化橘红中柚皮苷、柚皮芸香苷、野漆树苷和柚皮素的HPLC测定[J].山东中医杂志,2012,31(4):277-278.
[9]陆兔林,石上梅,蔡宝昌,等.基于一测多评的中药多成分定量研究进展[J].中草药,2012,43(12):2525-2529.
[10]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-658.
[11]袁旭江,李春阳,张平.一测多评法测定鸡骨草叶中3种黄酮碳苷含量[J].中药新药与临床药理,2014,25(4):493-518.
[12]万青,涂楚月,熊慧,等.一测多评法测定藏药金腰草中4种黄酮类化学成分的含量[J].药物分析杂志,2016,36(6):1053-1058.
[13]吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类成分[J].中草药,2017,48(4):777-781.
[14]林励,李向明,万建义,等.化橘红药材质量评价、监测与应用研究[J].中国现代中药,2010,12(8):21-26.