基于现代文献探索大鼠肥胖模型建立的优选方案研究
|
摘要
目的基于大数据挖掘技术,探索现代大鼠肥胖模型建立的优选方案。方法运用数据库检索的方法,以"大鼠""肥胖""模型建立"(包括"造模""建模")为关键词,对1998-2017年的CNKI、WF、VIP三大数据库中大鼠肥胖模型建立的相关文献进行检索及梳理,筛选出有效文献104篇,对造模大鼠品系、产地、引种周龄、喂养饲料成分、饲料硬度处理、造模时长、药理补救方法、成功率等影响因素进行多维数据对比分析,探索大鼠肥胖模型建立的优选方案。结果综合分析得出,造模选用中国医科院研究所等培育出生3～4周、断乳后1周左右、适应性饲养3 d左右的SPF级雄性Wistar大鼠喂以作冷藏颗粒硬化处理的高蛋白(粗蛋白质24%、脂肪12%、碳水化合物42%、其他22%)饲料,必要时辅以氯丙嗪(齐齐哈尔第二制药有限公司H23020442)[1]。结论经4～6周造模,为最优大鼠肥胖模型建立方案,成功65.0%～75.0%。
Objective Based on the data mining technology, to explore the establishment of a modern obesity rat model of optimal scheme. Methods With the keywords of "rat", "obesity" and "model building", the related literatures of rat obesity model in CNKI, WF and VIP databases from 1998 to 2017 were searched and sorted out by using database retrieval method. A total of 104 effective literatures were screened out. The influencing factors such as strains, and weeks of age, feed composition, feed processing hardness, molding time, pharmacological remedy, the success rate and influence factors were compared and analyzed by multi-dimensional data, and the optimal scheme for establishing rat obesity model was explored. Results Comprehensive analysis, model selection of China medical institutes to cultivate born 3～4 weeks, about 1 week after weaning, adaptive feeding about 3 days male Wistar SPF rats were fed with high protein cold hardening(coarse particles 24% protein, 12% fat, 42% carbohydrate, 22% other) feed, other necessary chlorpromazine(Qigihar Second Pharmaceutical Co. Ltd. H23020442), the 4～6 weeks model, to establish the rat model of the optimal scheme for obesity, successful 65.0%～75.0%. Conclusion Modeling 4～6 weeks was the best way to establish obesity model in rats. The success rate can achieve 65.0% ～ 75.0%.
Objective Based on the data mining technology, to explore the establishment of a modern obesity rat model of optimal scheme. Methods With the keywords of "rat", "obesity" and "model building", the related literatures of rat obesity model in CNKI, WF and VIP databases from 1998 to 2017 were searched and sorted out by using database retrieval method. A total of 104 effective literatures were screened out. The influencing factors such as strains, and weeks of age, feed composition, feed processing hardness, molding time, pharmacological remedy, the success rate and influence factors were compared and analyzed by multi-dimensional data, and the optimal scheme for establishing rat obesity model was explored. Results Comprehensive analysis, model selection of China medical institutes to cultivate born 3～4 weeks, about 1 week after weaning, adaptive feeding about 3 days male Wistar SPF rats were fed with high protein cold hardening(coarse particles 24% protein, 12% fat, 42% carbohydrate, 22% other) feed, other necessary chlorpromazine(Qigihar Second Pharmaceutical Co. Ltd. H23020442), the 4～6 weeks model, to establish the rat model of the optimal scheme for obesity, successful 65.0%～75.0%. Conclusion Modeling 4～6 weeks was the best way to establish obesity model in rats. The success rate can achieve 65.0% ～ 75.0%.
引文
[1]李媛,王保芝,岂春晓,等.一种新的肥胖大鼠模型的制备方法[J].河北医科大学学报, 2005, 26(4):305-307.
[2]李晓东.食源性肥胖大鼠模型的建立和电针治疗肥胖症作用机制的实验研究[D].天津:天津医科大学, 2003.
[3] WANG C R, TAN J, YANG K L. Establishment of a rat model of obesity induced by high fat diet and indirect estimation of body fat[J]. Journal of Beijing Sport University, 1994(3):20-23.
[4]王根辈,栗志文,曹晶,等.高脂饮食诱发大鼠营养性肥胖动物模型的研究[J].吉林医学, 2012(1):5-7.
[5]姚荣英,郑玲,李霞,等.营养性幼年肥胖雌性大鼠模型的建立[J].现代预防医学, 2012(3):558-559.
[6]徐姝迪,郑玉建,丁红,等.高脂饲料诱导肥胖大鼠模型的方法研究[J].现代预防医学, 2012(2):294-295.
[7]田小梅,陈梅晞.混合喂养法诱导营养性肥胖大鼠模型的实验研究[J].实用心脑肺血管病杂志, 2012(3):430-432.
[8]丁婧,王辉,余诗灏,等.肥胖大鼠模型的建立及其脂代谢相关分子机制研究[J].中国实验动物学报, 2012(5):20-24, 94.
[9]张建刚,米生权,孙晓玮,等.混合喂养法建立营养性肥胖大鼠模型[J].中国比较医学杂志, 2008(9):16-20, 90.
[10] WANG T Y, ZAN Y X, DONG W H, et al. High fat diet induced obesity rat model[J]. experimental study of modern preventive medicine, 2008(16):3131-3132.
[11] LI X, ZHAO D, NING H, et al. Study on the improvement of the experimental animal model of reducing weight[J]. Health Research, 2010(2):162-164.
[12]汤锦花,严海东.营养性肥胖大鼠模型的建立及评价[J].同济大学学报(医学版), 2010(1):32-34.
[13]郑绪阳,黄先玫.建立雄性营养性肥胖幼鼠模型的研究[J].中国预防医学杂志, 2011(2):146-148.
[14]赵玉琼.肥胖大鼠模型[J].实验动物科学, 2011(6):59-61.
[15]朱磊,路瑛丽,冯连世,等.不同配方高脂饲料构建SD大鼠肥胖模型的实验研究[J].中国运动医学杂志,2016(7):642-647.
[16]申瑞玲,马婧.四种肥胖动物造模方法的比较[J].动物医学进展, 2007(3):105-108.
[17]程志清,黄科.高血压合并肥胖大鼠动物模型的建立[J].浙江中医药大学学报, 2007(3):291-292.
[18]钱伯初,史红,吕燕萍.肥胖动物模型的研究进展[J].中国新药杂志, 2007(15):1159-1162.
[19]章涛,张潜,薛黔,等. SD大鼠肥胖动物模型的建立[J].遵义医学院学报, 2007(3):240-242.
[20]李晓静,刘敏,王安平,等.混合喂养法建立成年营养性肥胖大鼠模型[J].现代生物医学进展, 2015(18):3418-3421.
[21]陈凤丽,曹晨,孙亦农,等.高脂饲料诱发大鼠肥胖模型的实验研究[J].辽宁中医杂志, 2015(9):1792-1794.
[22]潘玲,李德良,张立群.成年营养性肥胖大鼠模型[J].中药药理与临床, 2003(5):47-49.
[23]王从容,谭健,杨锡让.高脂饲料诱发大鼠肥胖模型的建立及间接推测体脂法[J].北京体育大学学报, 1994(3):20-23.
[24]林帆,钱镭等高脂饮食诱导肥胖大鼠模型的实验研究[J].福建医药杂志, 2009(6):1-3.
[25] LU X L, LUO J Y, HU C G, et al. Journal of three rat models of fatty liver[J]. of Gastroenterology and Hepatology,2005(3):243-248.
[26] YANG A J, CUI Y, YE H Z. Establishment of an animal model of nutritional obesity[J]. Journal of clinical and experimental medicine, 2005(3):156-157.
[27]刘芳.不同品系小鼠肥胖模型比较、肥胖机制研究&LRTG减肥作用及作用机理探讨[D].北京:北京协和医学院,2013.
[28]丁婧.肥胖大鼠模型的建立及其发病的分子机制研究[D].北京:北京协和医学院, 2010.
[29]饶子亮,钟志勇,谢超敏,等.高脂饲料诱导肥胖大鼠和肥胖小鼠模型的比较研究[A].中国实验动物学会(Chinese Association for Laboratory Animal Sciences).第九届中国实验动物科学年会(2010新疆)论文集[C]//中国实验动物学会.
[30]刘晓平,王勇,龙廷,等.青春期单纯性肥胖雄性大鼠模型的实验研究[J].汕头大学医学院学报, 2006(3):160-161, 164.
[31]刘芳,高南南,杨润梅,等.不同品系小鼠肥胖模型比较及C57BL/6J小鼠肥胖机制研究[J].中国药理学通报,2013(3):360-365.
[32]田辉,王玉婷,陶莉,等.一种新型小鼠肥胖模型的建立[J].中国药理学通报, 2013(7):1016-1018.
[33]钱伯初.肥胖动物模型的制备原理和方法[J].中国药理学通报, 1993(1):75-76.
[34]刘恩岐,李亮平,师长宏.人类疾病动物模型[M].2版.北京:人民卫生出版社, 2014.
[2]李晓东.食源性肥胖大鼠模型的建立和电针治疗肥胖症作用机制的实验研究[D].天津:天津医科大学, 2003.
[3] WANG C R, TAN J, YANG K L. Establishment of a rat model of obesity induced by high fat diet and indirect estimation of body fat[J]. Journal of Beijing Sport University, 1994(3):20-23.
[4]王根辈,栗志文,曹晶,等.高脂饮食诱发大鼠营养性肥胖动物模型的研究[J].吉林医学, 2012(1):5-7.
[5]姚荣英,郑玲,李霞,等.营养性幼年肥胖雌性大鼠模型的建立[J].现代预防医学, 2012(3):558-559.
[6]徐姝迪,郑玉建,丁红,等.高脂饲料诱导肥胖大鼠模型的方法研究[J].现代预防医学, 2012(2):294-295.
[7]田小梅,陈梅晞.混合喂养法诱导营养性肥胖大鼠模型的实验研究[J].实用心脑肺血管病杂志, 2012(3):430-432.
[8]丁婧,王辉,余诗灏,等.肥胖大鼠模型的建立及其脂代谢相关分子机制研究[J].中国实验动物学报, 2012(5):20-24, 94.
[9]张建刚,米生权,孙晓玮,等.混合喂养法建立营养性肥胖大鼠模型[J].中国比较医学杂志, 2008(9):16-20, 90.
[10] WANG T Y, ZAN Y X, DONG W H, et al. High fat diet induced obesity rat model[J]. experimental study of modern preventive medicine, 2008(16):3131-3132.
[11] LI X, ZHAO D, NING H, et al. Study on the improvement of the experimental animal model of reducing weight[J]. Health Research, 2010(2):162-164.
[12]汤锦花,严海东.营养性肥胖大鼠模型的建立及评价[J].同济大学学报(医学版), 2010(1):32-34.
[13]郑绪阳,黄先玫.建立雄性营养性肥胖幼鼠模型的研究[J].中国预防医学杂志, 2011(2):146-148.
[14]赵玉琼.肥胖大鼠模型[J].实验动物科学, 2011(6):59-61.
[15]朱磊,路瑛丽,冯连世,等.不同配方高脂饲料构建SD大鼠肥胖模型的实验研究[J].中国运动医学杂志,2016(7):642-647.
[16]申瑞玲,马婧.四种肥胖动物造模方法的比较[J].动物医学进展, 2007(3):105-108.
[17]程志清,黄科.高血压合并肥胖大鼠动物模型的建立[J].浙江中医药大学学报, 2007(3):291-292.
[18]钱伯初,史红,吕燕萍.肥胖动物模型的研究进展[J].中国新药杂志, 2007(15):1159-1162.
[19]章涛,张潜,薛黔,等. SD大鼠肥胖动物模型的建立[J].遵义医学院学报, 2007(3):240-242.
[20]李晓静,刘敏,王安平,等.混合喂养法建立成年营养性肥胖大鼠模型[J].现代生物医学进展, 2015(18):3418-3421.
[21]陈凤丽,曹晨,孙亦农,等.高脂饲料诱发大鼠肥胖模型的实验研究[J].辽宁中医杂志, 2015(9):1792-1794.
[22]潘玲,李德良,张立群.成年营养性肥胖大鼠模型[J].中药药理与临床, 2003(5):47-49.
[23]王从容,谭健,杨锡让.高脂饲料诱发大鼠肥胖模型的建立及间接推测体脂法[J].北京体育大学学报, 1994(3):20-23.
[24]林帆,钱镭等高脂饮食诱导肥胖大鼠模型的实验研究[J].福建医药杂志, 2009(6):1-3.
[25] LU X L, LUO J Y, HU C G, et al. Journal of three rat models of fatty liver[J]. of Gastroenterology and Hepatology,2005(3):243-248.
[26] YANG A J, CUI Y, YE H Z. Establishment of an animal model of nutritional obesity[J]. Journal of clinical and experimental medicine, 2005(3):156-157.
[27]刘芳.不同品系小鼠肥胖模型比较、肥胖机制研究&LRTG减肥作用及作用机理探讨[D].北京:北京协和医学院,2013.
[28]丁婧.肥胖大鼠模型的建立及其发病的分子机制研究[D].北京:北京协和医学院, 2010.
[29]饶子亮,钟志勇,谢超敏,等.高脂饲料诱导肥胖大鼠和肥胖小鼠模型的比较研究[A].中国实验动物学会(Chinese Association for Laboratory Animal Sciences).第九届中国实验动物科学年会(2010新疆)论文集[C]//中国实验动物学会.
[30]刘晓平,王勇,龙廷,等.青春期单纯性肥胖雄性大鼠模型的实验研究[J].汕头大学医学院学报, 2006(3):160-161, 164.
[31]刘芳,高南南,杨润梅,等.不同品系小鼠肥胖模型比较及C57BL/6J小鼠肥胖机制研究[J].中国药理学通报,2013(3):360-365.
[32]田辉,王玉婷,陶莉,等.一种新型小鼠肥胖模型的建立[J].中国药理学通报, 2013(7):1016-1018.
[33]钱伯初.肥胖动物模型的制备原理和方法[J].中国药理学通报, 1993(1):75-76.
[34]刘恩岐,李亮平,师长宏.人类疾病动物模型[M].2版.北京:人民卫生出版社, 2014.