银屑平丸对小鼠银屑病样模型血清中TGF-β、IL-10表达的影响
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摘要
目的:观察银屑平丸对BALB/c小鼠银屑病样模型血清中转化生长因子-β(TGF-β)和白细胞介素10(IL-10)表达的影响,探讨银屑平丸治疗银屑病的可能作用机制。方法:将48只BALB/c雄性小鼠随机分为空白组、模型对照组、银屑平丸高、中、低剂量组、雷公藤组,每组8只。所有小鼠背部脱毛后,空白组小鼠外涂凡士林,其余5组小鼠外涂咪喹莫特乳膏,诱发小鼠银屑病样模型;同时予以相应药物进行灌胃干预,连续8 d。观察并记录小鼠背部皮肤皮损面积和严重程度指数(psoriasis area and serenty index,PASI)评分;ELISA法检测小鼠外周血清中TGF-β、IL-10水平;取小鼠背部皮肤组织做病理切片进行组织病理观察。结果:银屑平丸高、中、低剂量组、雷公藤组小鼠背部皮损PASI评分及外周血TGF-β、IL-10含量均明显低于模型对照组,差异有统计学意义(P<0.05)。结论:银屑平丸能降低银屑病样模型小鼠外周血TGF-β、IL-10的表达,这可能是银屑平丸治疗银屑病的作用机制之一。
Objective: To observe the effect of Yinxieping pills(银屑平丸) on TGF-β, IL-10 expression in serum of psoriasis-like BALB/c mice models, and to explore the possible mechanism of the Yinxieping pills on treating psoriasis. Methods: The 48 male BALB/c mice were randomly divided into 6 groups which 8 mice in each group. After all the mice were depilated on the back, the back skin were given with imiquimod cream except the blank group with vaseline, the rat models were established. At the same time, the corresponding drugs were given by gavage. The psoriasis area and severity index(PASI) score of the skin in the back of mice was observed. The levels of TGF-β and IL-10 in serum were detected. The skin tissue of the back were taken as pathological sections to compare the histopathology. Results: The PASI scores of back skin lesions and the levels of TGF-β and IL-10 in peripheral blood of mice in high, medium and low dose groups and Tripterygium wilfordii groups were significantly lower than those in model control group(P<0.05). Conclusion: Yinxieping Pills can reduce the content of TGF-β and IL-10 in psoriasis-like mice's peripheral blood, it is probably one of the mechanisms of Yinxieping pills in the treatment of psoriasis.
Objective: To observe the effect of Yinxieping pills(银屑平丸) on TGF-β, IL-10 expression in serum of psoriasis-like BALB/c mice models, and to explore the possible mechanism of the Yinxieping pills on treating psoriasis. Methods: The 48 male BALB/c mice were randomly divided into 6 groups which 8 mice in each group. After all the mice were depilated on the back, the back skin were given with imiquimod cream except the blank group with vaseline, the rat models were established. At the same time, the corresponding drugs were given by gavage. The psoriasis area and severity index(PASI) score of the skin in the back of mice was observed. The levels of TGF-β and IL-10 in serum were detected. The skin tissue of the back were taken as pathological sections to compare the histopathology. Results: The PASI scores of back skin lesions and the levels of TGF-β and IL-10 in peripheral blood of mice in high, medium and low dose groups and Tripterygium wilfordii groups were significantly lower than those in model control group(P<0.05). Conclusion: Yinxieping Pills can reduce the content of TGF-β and IL-10 in psoriasis-like mice's peripheral blood, it is probably one of the mechanisms of Yinxieping pills in the treatment of psoriasis.
引文
[1]席建元,陈偶英,田优德.银屑平胶囊治疗寻常型银屑病40例临床观察[J].新中医,2008,40(11):29-30.
[2]谢汶芳,席建元,李小鹏,等.银屑平丸对BALB/c小鼠银屑病样模型IL-17、IL-23表达的影响[J].湖南中医药大学学报,2016,36(5):27-31.
[3]李小鹏,席建元,谢汶芳,等.银屑平丸对豚鼠银屑病样模型血管内皮生长因子和肿瘤坏死因子-α表达的影响[J].中国中西医结合皮肤性病学杂志,2014,13(5):294-297.
[4]席建元,温柔,陈偶英,等.基于STAT3/Survivin信号通路探讨银屑平丸对寻常型银屑病的干预作用[J].中医药导报,2016,22(11):20-23.
[5] Van der Fits L, Mourits S, Voerman JS, et al. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis[J].J Immunol,2009,182(9):5836-5845.
[6] Van Belle A B, de Heusch M, Lemaire M M, et al.IL-22 is required for imiquimod-induced psoriasiform skin inflammation in mice[J]. J Immunol,2012,188(1):462-469.
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[8]李冰.调节性T细胞在银屑病中的作用及机制研究[D].西安:第四军医大学,2016.
[9]徐文俊,毛常亮,冯仁洋,等.寻常型银屑病(白疕)血热证与TH17相关细胞因子病理变化的相关性分析[J].中医药导报,2016,22(21):18-23.
[10]熊国欣,李新忠.准分子激光照射对寻常型银屑病患者外周血Th17/treg失衡及相关细胞因子表达的影响[J].应用激光,2015,35(2):264-268.
[11]张玉丽,王逸飞,张春敏.CD4+CD25+调节性T细胞与Th17细胞在寻常型银屑病中的表达[J].中国麻风皮肤病杂志,2016,32(11):653-656.
[12]韩凌,颜克香,钱辉,等.CD39和CD73在CD4+CD25highFoxp3+调节性T细胞发挥免疫抑制功能中的作用研究[J].现代免疫学,2017,37(2):101-106.
[13]梅沉成,张云璧,陈曦,等.Treg/Th17平衡偏移与寻常型银屑病中医三证型的相关性研究[J].中华)中医药杂志,2017,32(6):2717-2720.
[14]李垣君.儿童银屑病与代谢综合征各组分的相关性研究及机制探讨[D].广州:暨南大学,2017.
[15] Yun K X,Fang X,Han L,et al. Foxp3+regulatory T cells and related cytokines differentially expressed on plaque vs. Guttate psoriasis volgaris[J].Br J Dermatol,2010,163(1):48-56.
[16] Zhang L,Yang X Q,Cheng J,et al. Inereased Th17 cells are accompanied by Foxp3 Treg cell accumulation and correlated with psoriasis disease severity[J].J Clin Immunol,2010,135(1):108-117.
[17] Li Zhang,Xue-Qin Yang,Juan Cheng,et al. Increased Th17 cells are accompanied by FoxP3+Treg cell accumulation and correlated with psoriasis disease severity[J].Clin Immunol,2010,135(1):108-117.
[18] Chen Ling,Shen Zhu,Wang Gang. Dynamic frequency of CD4+CD25+Foxp3+Treg cells in psoriasis vulgaris[J].J Dermatol Sci,2008,51(3):200-203.
[19]胡文娟.脾气虚证与寻常型银屑病的相关性研究[D].广州:广州中医药大学,2016.
[20]金权鑫,金桂花,金丹,等.咪喹莫特诱导的小鼠银屑病模型的建立[J].延边大学医学学报,2014,37(4):241-243.
[21] Sun J,Zhao Y,Hu J. Curcumin inhibits imiquimod-induced psoriasis-like inflammation by inhibiting IL-1beta and IL-6 production in mice[J].PloS One,2013,8(6):e67078.
[22]郭顺,时乐,闫小兵,等.麻桂汤对咪喹莫特诱导小鼠银屑病样皮损的干预研究[J].中药材,2014,37(6):1049-1052.
[23]张跃营,岳东山,苏晓明,等.贝伐珠单抗注射液在改善银屑病小鼠模型中的作用及机制[J].中国医院药学杂志,2017,37(14):1374-1378.
[24]张明瑞.α1抗胰蛋白酶对咪喹莫特诱导BABL/c小鼠银屑病样模型治疗作用及机制的实验研究[D].长春:吉林大学,2015.
[2]谢汶芳,席建元,李小鹏,等.银屑平丸对BALB/c小鼠银屑病样模型IL-17、IL-23表达的影响[J].湖南中医药大学学报,2016,36(5):27-31.
[3]李小鹏,席建元,谢汶芳,等.银屑平丸对豚鼠银屑病样模型血管内皮生长因子和肿瘤坏死因子-α表达的影响[J].中国中西医结合皮肤性病学杂志,2014,13(5):294-297.
[4]席建元,温柔,陈偶英,等.基于STAT3/Survivin信号通路探讨银屑平丸对寻常型银屑病的干预作用[J].中医药导报,2016,22(11):20-23.
[5] Van der Fits L, Mourits S, Voerman JS, et al. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis[J].J Immunol,2009,182(9):5836-5845.
[6] Van Belle A B, de Heusch M, Lemaire M M, et al.IL-22 is required for imiquimod-induced psoriasiform skin inflammation in mice[J]. J Immunol,2012,188(1):462-469.
[7]赵辨.中国临床皮肤病学[M].4版.南京:江苏科学技术出版社,2009:1011-1014.
[8]李冰.调节性T细胞在银屑病中的作用及机制研究[D].西安:第四军医大学,2016.
[9]徐文俊,毛常亮,冯仁洋,等.寻常型银屑病(白疕)血热证与TH17相关细胞因子病理变化的相关性分析[J].中医药导报,2016,22(21):18-23.
[10]熊国欣,李新忠.准分子激光照射对寻常型银屑病患者外周血Th17/treg失衡及相关细胞因子表达的影响[J].应用激光,2015,35(2):264-268.
[11]张玉丽,王逸飞,张春敏.CD4+CD25+调节性T细胞与Th17细胞在寻常型银屑病中的表达[J].中国麻风皮肤病杂志,2016,32(11):653-656.
[12]韩凌,颜克香,钱辉,等.CD39和CD73在CD4+CD25highFoxp3+调节性T细胞发挥免疫抑制功能中的作用研究[J].现代免疫学,2017,37(2):101-106.
[13]梅沉成,张云璧,陈曦,等.Treg/Th17平衡偏移与寻常型银屑病中医三证型的相关性研究[J].中华)中医药杂志,2017,32(6):2717-2720.
[14]李垣君.儿童银屑病与代谢综合征各组分的相关性研究及机制探讨[D].广州:暨南大学,2017.
[15] Yun K X,Fang X,Han L,et al. Foxp3+regulatory T cells and related cytokines differentially expressed on plaque vs. Guttate psoriasis volgaris[J].Br J Dermatol,2010,163(1):48-56.
[16] Zhang L,Yang X Q,Cheng J,et al. Inereased Th17 cells are accompanied by Foxp3 Treg cell accumulation and correlated with psoriasis disease severity[J].J Clin Immunol,2010,135(1):108-117.
[17] Li Zhang,Xue-Qin Yang,Juan Cheng,et al. Increased Th17 cells are accompanied by FoxP3+Treg cell accumulation and correlated with psoriasis disease severity[J].Clin Immunol,2010,135(1):108-117.
[18] Chen Ling,Shen Zhu,Wang Gang. Dynamic frequency of CD4+CD25+Foxp3+Treg cells in psoriasis vulgaris[J].J Dermatol Sci,2008,51(3):200-203.
[19]胡文娟.脾气虚证与寻常型银屑病的相关性研究[D].广州:广州中医药大学,2016.
[20]金权鑫,金桂花,金丹,等.咪喹莫特诱导的小鼠银屑病模型的建立[J].延边大学医学学报,2014,37(4):241-243.
[21] Sun J,Zhao Y,Hu J. Curcumin inhibits imiquimod-induced psoriasis-like inflammation by inhibiting IL-1beta and IL-6 production in mice[J].PloS One,2013,8(6):e67078.
[22]郭顺,时乐,闫小兵,等.麻桂汤对咪喹莫特诱导小鼠银屑病样皮损的干预研究[J].中药材,2014,37(6):1049-1052.
[23]张跃营,岳东山,苏晓明,等.贝伐珠单抗注射液在改善银屑病小鼠模型中的作用及机制[J].中国医院药学杂志,2017,37(14):1374-1378.
[24]张明瑞.α1抗胰蛋白酶对咪喹莫特诱导BABL/c小鼠银屑病样模型治疗作用及机制的实验研究[D].长春:吉林大学,2015.