胚挽救技术在葡萄育种上的研究及应用
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摘要
胚挽救是指受精后胚在早期的发育阶段退化或败育,然后通过早期胚的离体培养而发育成完整植株的过程。胚挽救技术在早熟葡萄育种率的提高、无核葡萄品种的培育、三倍体葡萄的育种以及葡萄远缘杂交等方面发挥着重要作用。综述了胚挽救的概念、影响胚挽救成功的因素、胚挽救技术在葡萄育种中的应用,并提出了胚挽救技术在今后的研究和应用前景。
Embryo rescue refers to the process by which post-fertilization embryos degenerate or abort at an early developmental stage and then develop into intact plants by in vitro culture of early embryos. Embryo rescue technology plays an important role in the improvement of early maturing grape breeding rate, the cultivation of seedless grape varieties, the breeding of triploid grapes, and the distant hybridization of grapes. This paper reviews the concept of embryo rescue, the factors affecting the success of embryo rescue, the application of embryo rescue technology in grape breeding, and the future research and application prospects of embryo rescue technology are put forward.
Embryo rescue refers to the process by which post-fertilization embryos degenerate or abort at an early developmental stage and then develop into intact plants by in vitro culture of early embryos. Embryo rescue technology plays an important role in the improvement of early maturing grape breeding rate, the cultivation of seedless grape varieties, the breeding of triploid grapes, and the distant hybridization of grapes. This paper reviews the concept of embryo rescue, the factors affecting the success of embryo rescue, the application of embryo rescue technology in grape breeding, and the future research and application prospects of embryo rescue technology are put forward.
引文
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[2]SNYDER E,HARMON F N.Grape breeding summary 1932-1952[J].Proc Amer Soc Hort Sci,1952,60:243-246.
[3]RAMMING D W,EMERSHAD R L.In ovule embryo culture of seeded and seedless Vitis vinifera L.(Abst.)[J].Hortscience,1982,17:487.
[4]RAMMING D W,EMERSHAD R L.Stemospernocarpic seedless Vitis vinifera×Vitis rotundifolia hybrid developed by embryo rescue[J].Hortscience,2000,35(4):732-734.
[5]BHARATHY P V,KARl BASAPPA G S,PATIL S G,et a1.In ovule rescue of hybrid embryos in Flame seedless grapes-influence of pre-bloom sprays of benzyladenine[J].Scientia Horticulturae,2005,106:353-359.
[6]AMARALA L,OLIVEIRA P R,CZERAINSKI A B,et al.Embryo growth stages on plant obtention from crosses between seedless grape parents[J].Rev Bras Frutic,2001,23(3):647-651.
[7]GRAY D J,MORTENSEN J A,BENTON C M.Ovule Culture to obtain progeny from hybrid seedless bunch grapes[J].J Amer Soc Hort Sci,1990,115(6):1019-1024.
[8]GOLDY R G,EMERSHAD R L.Embryo culture as a means of introgressory seedlessness from Vitis vinifera to V.rotundifolia[J].Hortscience,1988,23(5):886-889.
[9]EMERSHAD R L,RAMMING D W.Somatic embryo genesis and p lant development from immature zygotic embryos of seedless grapes(Vitis vinifera L.)[J].Plant Cell Reports,1994,14:6-12.
[10]GRAY D J.亓桂梅,译.利用组织培养方法从无核葡萄杂交中获得后代[J].葡萄栽培与酿酒,1993,6(3):35-38.
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[12]LEDBETTER C A,SHONNERD C B.Improved seed development and germination of stenospermic grapes by plant growth regulators[J].Horticultured Sci,1990,65(3):269-274.
[13]孟新法,张利,张潞生,等.无核葡萄胚发育及早期离体培养的研究III.培养方式对离体胚发育影响[J].北京农业大学学报,1992,18(4):393-395.
[14]孟新法,张利,张潞生,等.无核葡萄胚发育及早期离体培养的研究IV.胚珠接种时期对胚发育的影响[J].北京农业大学学报,1993,19(1):45-47.
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[18]亓桂梅,杨立英.植物生长调节剂对假单性结实葡萄种子发育和萌发的影响[J].山东农业科学,2001,32(5):21-22.
[19]DING H F,QI G M.Ovules culture and plant formation of hybrid progeny of seedless grape[J].Journal of Agriculture in the Tropics and Subtropics,2001,102(2):147-152.
[20]董晓玲,贺普超.无核葡萄杨格尔的胚发育及胚培养[J].果树科学,1991,8(2):55-58.
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[22]李桂荣,王跃进,唐冬梅,等.无核白葡萄胚挽救育种技术研究[J].西北植物学报,2001,21(3):432-436.
[23]王飞,王跃进,周建锡.无核葡萄与中国野生葡萄杂种的胚挽救技术研究[J].园艺学报,2006,33(5):1079-1082.
[24]刘加驹,卢春生.无核葡萄[J].果树科学,1985(1):15-20.
[25]张潞生,孟新法.大粒无核葡萄品种选育技术[J].北京农业大学学报,1993,19(4):92-97.
[26]SPIEGEL-ROY,SAHAR P N,BARON J,et al.In vitro culture and plant formation from grape culture with abortive ovules and seeds[J].Amer Soc Hort Sci,1985,110:109-112.
[27]GO1DY R G.In vitro cultivability of ovules from 10 seedless grape clones[J].Hortsciene,1987,22(5):952.
[28]马远,张振文,杨玉洁,等.葡萄组织培养应用研究进展[J].中外葡萄与葡萄酒,2002(4):23-26.
[29]CAIN,D W,EMERSHAD R L,TARAILO R E.In-ovulo embryo culture and seedling development of seeded and seedless grapes(Vitis vinifera L.)[J].Vitis,1983,22:9-14.
[30]EMERSHAD R L,RAMMING D W,SERPE M D.In ovule plant formation from stenospermic genotypes of Vitis vinifera[J].L Amer J Bot,1989,76(3):397-402.
[31]王跃进,张剑侠,李桂荣,等.采用胚挽救技术获得抗病无核葡萄新材料的研究[J].甘肃农业大学学报,2001,36(专辑):105-111.
[32]ALBERTO C,SUZANNE M,PETHIER R.By growth of immature peach embryos in response to media,ovule support method,and ovule perforation[J].Hortsience,1994,29(9):1081-1083.
[33]GRIBAUDO I,ZANETTI R,BOTTA R,et al.I.In ovule embryo culture of stenospermocarpic grapes[J].Vitis,1993,32:9-14.
[34]贺普超,董晓玲.极早熟葡萄胚珠培养技术的研究[J].果树科学,1989,6(4):199-204.
[35]陈香波,曹孜义.离体葡萄未成熟胚成苗途径研究[J].果树科学,2000,17(4):261-264.
[36]颜昌敬.植物组织培养手册[M].上海:上海科学技术出版社,1990.
[37]AG譈ERO C,GERGORI M T,PONCE M T,et al.An improved germination of stenospermic grape fertilized ovules cultured by low temperature[J].Biocell,1996,20(2):123-126.
[38]BURGER P,TRAUTMANN I A.Manipulations of ovules to improve in vitro development of Vitis vinifera L.embryos[J].Acta Horticulturae,2000,528:613-619.
[39]GARCIA E,MARTINEZ A,GARCIA DE LA CALERA E,et al.In vitro culture of ovules and embryos of grape for the obtain of new seedless table grape cultivars[J].Acta Horticulturae,2000,528:663-666.
[40]郭印山,张海娥,郭修武,等.早熟葡萄胚抢救技术研究[J].中外葡萄与葡萄酒,2006(1):11-15.
[41]RAMMING D W,EMERSHAD R L.Embryo culture of early ripening seeded grape(Vitis vinifera)genotypes[J].Hort scince,1990,25(3):339-342.
[42]SHARMA DR,KAUR R,KUMAR K.Embryo rescue in plants-a review[J].Euphytica,1996,89:325-337.
[43]BOUQUET A,DAVIS H P.In vitro ovule and embryo culture for breeding seedless table grapes(Vitis vinifera L.)[J].Agronomie,1989,9:565-574.
[44]RAMMING D W.The use of embryo culture in fruit breeding[J].Hortscience,1990,25(4):393-398.
[45]NOTSUKA K,TSURU T,SHIRAISHI M.Seedless-seedless grape hybridization via in ovule embryo culture[J].Jap Soc Hortic Sci,2001,70:7-15.
[46]郭修武,郭印山,张海娥,等.接种时期和培养基对无核葡萄胚挽救的影响[J].园艺学报,2007,34(2):329-332.
[47]GOLDY R G,Ramming D W,Emershad R L,Chaparro J X.Increasing production of Vitis vinifera×V.rotundifolia hybrids through embryo rescue[J].Hortscience,1989(24):820-822.
[48]潘春云,亓桂梅,汤小宁,等.葡萄三倍体育种初报[J].山东农业大学学报,1998,29(3):299-302.
[49]YAMASHITA H,HORIUCHI S,TAIRA T.Development of seeds and the growth of triploid seedlings obtained from reciprocal crosses between diploids and triploid grapes[J].Jap Soc Hortic Sci,1993,62:249-255.
[50]YAMASHITA H,HANIUDA T,SHIBA H.In vitro culture of embryos obtained by crossing tetraploid grape cultivar Kyoho with diploid cultivars[J].Jap Soc Hortic Sci,1995,63:719-724.
[51]YAMASHITA H,SHIGEHARA I,HANIUDA T.Production of triploid grapes by in ovulo embryo culture[J].Vitis,1998,37:113-117.
[52]亓桂梅.无核葡萄杂交胚珠的培养及萌发成苗的若干影响因素[J].中外葡萄与葡萄酒,2001(4):24-27.
[53]徐海英,阎爱玲,张国军.葡萄二倍体与四倍体品种间杂交胚珠的离体培养[J].果树学报,2001,18(6):317-320.
[54]王跃进,LAMIKANRA O.葡萄无核基因的RAPD标记的序列分析[J].西北农业大学学报,1997,25(4):l-5.
[55]刘小宁,王跃进,张剑侠,等.Flame Seedless葡萄胚珠、胚乳及胚发育与败育的研究[J].西北植物学报,2005,25(10):1947-1953.
[56]郝燕,王发林,杨瑞,等.无核葡萄胚败育时期的确定及接种时间对胚萌发的影响[J].甘肃农业大学学报,2006,41(4):45-48.
[57]蒋爱丽,李世诚,金佩芳,等.大败育型无核葡萄胚珠培养成苗技术研究[J].上海交通大学学报(农业科学版),2002,20(1):45-48.
[58]李世诚,金佩芳,蒋爱丽,等.二倍体与四倍体杂交的无核葡萄胚珠培养获得三倍体植株[J].上海农业学报,1998,14(4):13-17.
[2]SNYDER E,HARMON F N.Grape breeding summary 1932-1952[J].Proc Amer Soc Hort Sci,1952,60:243-246.
[3]RAMMING D W,EMERSHAD R L.In ovule embryo culture of seeded and seedless Vitis vinifera L.(Abst.)[J].Hortscience,1982,17:487.
[4]RAMMING D W,EMERSHAD R L.Stemospernocarpic seedless Vitis vinifera×Vitis rotundifolia hybrid developed by embryo rescue[J].Hortscience,2000,35(4):732-734.
[5]BHARATHY P V,KARl BASAPPA G S,PATIL S G,et a1.In ovule rescue of hybrid embryos in Flame seedless grapes-influence of pre-bloom sprays of benzyladenine[J].Scientia Horticulturae,2005,106:353-359.
[6]AMARALA L,OLIVEIRA P R,CZERAINSKI A B,et al.Embryo growth stages on plant obtention from crosses between seedless grape parents[J].Rev Bras Frutic,2001,23(3):647-651.
[7]GRAY D J,MORTENSEN J A,BENTON C M.Ovule Culture to obtain progeny from hybrid seedless bunch grapes[J].J Amer Soc Hort Sci,1990,115(6):1019-1024.
[8]GOLDY R G,EMERSHAD R L.Embryo culture as a means of introgressory seedlessness from Vitis vinifera to V.rotundifolia[J].Hortscience,1988,23(5):886-889.
[9]EMERSHAD R L,RAMMING D W.Somatic embryo genesis and p lant development from immature zygotic embryos of seedless grapes(Vitis vinifera L.)[J].Plant Cell Reports,1994,14:6-12.
[10]GRAY D J.亓桂梅,译.利用组织培养方法从无核葡萄杂交中获得后代[J].葡萄栽培与酿酒,1993,6(3):35-38.
[11]LEDBETTER C A,RAMMING D W.Seedlessnessingrapes[J].Horticultual Review,1989,11:159-181.
[12]LEDBETTER C A,SHONNERD C B.Improved seed development and germination of stenospermic grapes by plant growth regulators[J].Horticultured Sci,1990,65(3):269-274.
[13]孟新法,张利,张潞生,等.无核葡萄胚发育及早期离体培养的研究III.培养方式对离体胚发育影响[J].北京农业大学学报,1992,18(4):393-395.
[14]孟新法,张利,张潞生,等.无核葡萄胚发育及早期离体培养的研究IV.胚珠接种时期对胚发育的影响[J].北京农业大学学报,1993,19(1):45-47.
[15]张宏明,孟新法,张潞生.无核葡萄胚珠发育及早期离体培养的研究I.激素对胚珠离体培养的影响[J].北京农业大学学报,1990,16(3):227-282.
[16]张利,孟新法,张潞生,等.无核葡萄胚珠发育及早期离体培养的研究II.无核葡萄胚发育的特点[J].北京农业大学学报,1991,17(4):55-59.
[17]亓桂梅,潘春云,汤小宁,等.无核葡萄杂交胚珠的培养技术[J].果树科学,1998,15(4):364-367.
[18]亓桂梅,杨立英.植物生长调节剂对假单性结实葡萄种子发育和萌发的影响[J].山东农业科学,2001,32(5):21-22.
[19]DING H F,QI G M.Ovules culture and plant formation of hybrid progeny of seedless grape[J].Journal of Agriculture in the Tropics and Subtropics,2001,102(2):147-152.
[20]董晓玲,贺普超.无核葡萄杨格尔的胚发育及胚培养[J].果树科学,1991,8(2):55-58.
[21]郭海江,王跃进,张剑侠,等.葡萄抗病无核胚挽救育种及分子标记辅助选择[J].西北植物报,2005,25(2):2395-2401.
[22]李桂荣,王跃进,唐冬梅,等.无核白葡萄胚挽救育种技术研究[J].西北植物学报,2001,21(3):432-436.
[23]王飞,王跃进,周建锡.无核葡萄与中国野生葡萄杂种的胚挽救技术研究[J].园艺学报,2006,33(5):1079-1082.
[24]刘加驹,卢春生.无核葡萄[J].果树科学,1985(1):15-20.
[25]张潞生,孟新法.大粒无核葡萄品种选育技术[J].北京农业大学学报,1993,19(4):92-97.
[26]SPIEGEL-ROY,SAHAR P N,BARON J,et al.In vitro culture and plant formation from grape culture with abortive ovules and seeds[J].Amer Soc Hort Sci,1985,110:109-112.
[27]GO1DY R G.In vitro cultivability of ovules from 10 seedless grape clones[J].Hortsciene,1987,22(5):952.
[28]马远,张振文,杨玉洁,等.葡萄组织培养应用研究进展[J].中外葡萄与葡萄酒,2002(4):23-26.
[29]CAIN,D W,EMERSHAD R L,TARAILO R E.In-ovulo embryo culture and seedling development of seeded and seedless grapes(Vitis vinifera L.)[J].Vitis,1983,22:9-14.
[30]EMERSHAD R L,RAMMING D W,SERPE M D.In ovule plant formation from stenospermic genotypes of Vitis vinifera[J].L Amer J Bot,1989,76(3):397-402.
[31]王跃进,张剑侠,李桂荣,等.采用胚挽救技术获得抗病无核葡萄新材料的研究[J].甘肃农业大学学报,2001,36(专辑):105-111.
[32]ALBERTO C,SUZANNE M,PETHIER R.By growth of immature peach embryos in response to media,ovule support method,and ovule perforation[J].Hortsience,1994,29(9):1081-1083.
[33]GRIBAUDO I,ZANETTI R,BOTTA R,et al.I.In ovule embryo culture of stenospermocarpic grapes[J].Vitis,1993,32:9-14.
[34]贺普超,董晓玲.极早熟葡萄胚珠培养技术的研究[J].果树科学,1989,6(4):199-204.
[35]陈香波,曹孜义.离体葡萄未成熟胚成苗途径研究[J].果树科学,2000,17(4):261-264.
[36]颜昌敬.植物组织培养手册[M].上海:上海科学技术出版社,1990.
[37]AG譈ERO C,GERGORI M T,PONCE M T,et al.An improved germination of stenospermic grape fertilized ovules cultured by low temperature[J].Biocell,1996,20(2):123-126.
[38]BURGER P,TRAUTMANN I A.Manipulations of ovules to improve in vitro development of Vitis vinifera L.embryos[J].Acta Horticulturae,2000,528:613-619.
[39]GARCIA E,MARTINEZ A,GARCIA DE LA CALERA E,et al.In vitro culture of ovules and embryos of grape for the obtain of new seedless table grape cultivars[J].Acta Horticulturae,2000,528:663-666.
[40]郭印山,张海娥,郭修武,等.早熟葡萄胚抢救技术研究[J].中外葡萄与葡萄酒,2006(1):11-15.
[41]RAMMING D W,EMERSHAD R L.Embryo culture of early ripening seeded grape(Vitis vinifera)genotypes[J].Hort scince,1990,25(3):339-342.
[42]SHARMA DR,KAUR R,KUMAR K.Embryo rescue in plants-a review[J].Euphytica,1996,89:325-337.
[43]BOUQUET A,DAVIS H P.In vitro ovule and embryo culture for breeding seedless table grapes(Vitis vinifera L.)[J].Agronomie,1989,9:565-574.
[44]RAMMING D W.The use of embryo culture in fruit breeding[J].Hortscience,1990,25(4):393-398.
[45]NOTSUKA K,TSURU T,SHIRAISHI M.Seedless-seedless grape hybridization via in ovule embryo culture[J].Jap Soc Hortic Sci,2001,70:7-15.
[46]郭修武,郭印山,张海娥,等.接种时期和培养基对无核葡萄胚挽救的影响[J].园艺学报,2007,34(2):329-332.
[47]GOLDY R G,Ramming D W,Emershad R L,Chaparro J X.Increasing production of Vitis vinifera×V.rotundifolia hybrids through embryo rescue[J].Hortscience,1989(24):820-822.
[48]潘春云,亓桂梅,汤小宁,等.葡萄三倍体育种初报[J].山东农业大学学报,1998,29(3):299-302.
[49]YAMASHITA H,HORIUCHI S,TAIRA T.Development of seeds and the growth of triploid seedlings obtained from reciprocal crosses between diploids and triploid grapes[J].Jap Soc Hortic Sci,1993,62:249-255.
[50]YAMASHITA H,HANIUDA T,SHIBA H.In vitro culture of embryos obtained by crossing tetraploid grape cultivar Kyoho with diploid cultivars[J].Jap Soc Hortic Sci,1995,63:719-724.
[51]YAMASHITA H,SHIGEHARA I,HANIUDA T.Production of triploid grapes by in ovulo embryo culture[J].Vitis,1998,37:113-117.
[52]亓桂梅.无核葡萄杂交胚珠的培养及萌发成苗的若干影响因素[J].中外葡萄与葡萄酒,2001(4):24-27.
[53]徐海英,阎爱玲,张国军.葡萄二倍体与四倍体品种间杂交胚珠的离体培养[J].果树学报,2001,18(6):317-320.
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