葡萄籽提取物对人鼻咽癌细胞HIF-1α和VEGF表达的影响
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摘要
目的:研究葡萄籽提取物(GSE)对鼻咽癌(NPC)细胞HIF-1α和VEGF表达的影响,为GSE在NPC治疗中的应用提供依据。方法:采用100μmol/L CoCl_2建立细胞拟缺氧模型,用终浓度为0mg/L、12.5 mg/L、25 mg/L和50 mg/L的GSE加入鼻咽癌CNE-2Z细胞系,作用48h后,采用实时荧光定量PCR(Quantitative Real-time PCR)检测低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)m RNA表达,免疫印迹技术(Western Blot)检测HIF-1α和VEGF表达。结果:低氧环境下,不同浓度GSE干预抑制鼻咽癌细胞HIF-1α和VEGF表达均受到显著影响。结论:GSE对鼻咽癌CNE-2Z细胞HIF-1α和VEGF表达具有调节作用。
Objective:To investigate the effect ofgrape seed extract(GSE)on expression of HIF-1α and VEGF gene in nasopharyngeal carcinoma cell line CNE-2Z. Methods:The model of hypoxia was established by 100μmol/L CoCl_2,The GSE with different final concentration of 0mg/L,12.5mg/L,25mg/L and 50mg/L was added to CNE-2Z cells cultured in vitro for succedent experiments.The experimental and blank control groups of CNE-2Z cells were cultivated for 48 h.The expression of HIF-1α and VEGF gene was detected by Quantitative Real-time PCR and Western Blot at m RNA and protein level. Results:Under hypoxic conditions,different solubility of GSE intervention on nasopharyngeal carcinoma cells HIF-1α and VEGF expression were significantly affected. Conclusion:GSE has a regulatory effect on the expression of HIF-1α and VEGF in CNE-2Z cells of nasopharyngeal carcinoma.
Objective:To investigate the effect ofgrape seed extract(GSE)on expression of HIF-1α and VEGF gene in nasopharyngeal carcinoma cell line CNE-2Z. Methods:The model of hypoxia was established by 100μmol/L CoCl_2,The GSE with different final concentration of 0mg/L,12.5mg/L,25mg/L and 50mg/L was added to CNE-2Z cells cultured in vitro for succedent experiments.The experimental and blank control groups of CNE-2Z cells were cultivated for 48 h.The expression of HIF-1α and VEGF gene was detected by Quantitative Real-time PCR and Western Blot at m RNA and protein level. Results:Under hypoxic conditions,different solubility of GSE intervention on nasopharyngeal carcinoma cells HIF-1α and VEGF expression were significantly affected. Conclusion:GSE has a regulatory effect on the expression of HIF-1α and VEGF in CNE-2Z cells of nasopharyngeal carcinoma.
引文
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[3]Cheah KY,Howarth GS,Bastian SE.Grape seed extract dose-responsively decreases disease severity in a rat model of mucositis;concomitantlyenhancingchemotherapeutic effectiveness in colon cancer cells[J].PLo S One,2014,9(1):e85184.
[4]Dinicola S,Pasqualato A,Cucina A,et al.Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion[J].Eur JNutr,2014,53(2):421-431.
[5]Prasad R,Katiyar SK.Grape seed proanthocyanidins inhibit migration potential of pancreatic cancer cells by promoting mesenchymal-to-epithelial transition and targeting NF-κB[J].Cancer Lett,2013,334(1):118-126.
[6]Lafaro KJ,Demirjian AN,Pawlik TM.Epidemiology of hepato-cellular carcinoma[J].Surg Oncol Clin N Am,2015,24(1):1-17.
[7]Song IS,Wang AG,Yoon SY,et a1.Regulation of glucose metabolismrelated genes and VEGF by HIF-1 alpha and HIF-1 beta,but not HIF-2 alpha,in gastric cancer[J].Exp Mol Med,2009,41(1):51-58.
[8]Sung FL,Hui EP,Tao Q,et al.Genome-wide expression analysis using microarray identified complex signaling pathways modulated by hypoxia in nasopharyngeal carcinoma[J].Cancer Lett,2007,253(1):74-88.
[9]Shou Z,Lin L,Liang J,et al.Expression and prognosis of FOXO3a and HIF-1αin nasopharyngeal carcinoma[J].J Cancer Res Clin Oncol,2012,138(4):585-93.
[10]曾伟,张建国.siRNA沉默HIF-1α在缺氧状态下对鼻咽癌细影响[J].现代中西医结合杂志,2009,18(21):2499-2505.