铁线莲2个野生种花粉活力和柱头可授性
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摘要
以野生的大花铁线莲(Clematis patens)和褐毛铁线莲(Clematis fusc)为研究对象,对其不同花期、不同开花时间和不同保存温度下的花粉活力进行研究,同时研究了2种铁线莲在不同开花时间的柱头可授性,探讨了成功授粉时期的选择。结果表明:2种铁线莲均在初花期和盛花期具有较高的花粉活力,大花铁线莲开花第3天花粉能保持很高的活力,褐毛铁线莲则能保持到第6天,2种铁线莲花粉适合低温保存,4℃下能较长时间保持较高的花粉活力;大花铁线莲柱头可授性能保持到开花第9天,褐毛铁线莲只能保持到第7天,大花铁线莲花粉活力较低时柱头仍具有较强的可授性,而褐毛铁线莲在柱头可授性基本消失时花粉仍具有很高的活性,为研究2种铁线莲的传粉方式提供了参考,有助于开发优质的铁线莲植物种质资源。
The experiment was conducted to study the pollen viability under different flowering periods,different flowering days,different storage temperatures and different flowering days of two clematis,stigma receptivity of Clematis patens and C. fusc in Yantong Mountains,in China. At the same time,selection of successful pollination period was also analyzed. The both clematis had higher pollen vigor at the early flowering stage and full flowering stage,and the pollen of the third flowering day of C. patens maintained high vigor,while C. fusc maintained by the sixth day,and two types of clematis were suitable for low temperature preservation and could maintain high pollen viability at 4 ℃ for a long time; the stigma receptivity of C.patens maintained until the ninth day of flowering,C. fusc only maintained until the seventh day,when the pollen viability of C. patens was low,the stigma still had strong receptivity,and the pollen had high activity when the receptivity of the stigma was basically disappeared for C. fusc.
The experiment was conducted to study the pollen viability under different flowering periods,different flowering days,different storage temperatures and different flowering days of two clematis,stigma receptivity of Clematis patens and C. fusc in Yantong Mountains,in China. At the same time,selection of successful pollination period was also analyzed. The both clematis had higher pollen vigor at the early flowering stage and full flowering stage,and the pollen of the third flowering day of C. patens maintained high vigor,while C. fusc maintained by the sixth day,and two types of clematis were suitable for low temperature preservation and could maintain high pollen viability at 4 ℃ for a long time; the stigma receptivity of C.patens maintained until the ninth day of flowering,C. fusc only maintained until the seventh day,when the pollen viability of C. patens was low,the stigma still had strong receptivity,and the pollen had high activity when the receptivity of the stigma was basically disappeared for C. fusc.
引文
[1]蔡艳飞,李世峰,李涵,等.中国铁线莲属植物研究进展[J].中国农学通报,2009,25(4):195-198.
[2]王文采,李良千.铁线莲属:新分类系统[J].植物分类学报,2005,43(5):431-488.
[3]钱仁卷,郑坚,胡青荻,等.中国铁线莲属观赏种质资源研究进展[J].中国农学通报,2017,33(21):75-81.
[4]吴荣.观赏铁线莲离体培养研究[D].昆明:西南林业大学,2011.
[5]余伟军.铁线莲遗传多样性、杂交育种及离体快繁研究[D].福州:福建农林大学,2017.
[6]阮丽丽.铁线莲属植物的杂交和扦插研究[C]//张启祥.中国观赏园艺研究进展.北京:中国林业出版社,2014.
[7]苏芸芸,王康才,薛启.不同产地藿香花粉活力与柱头可授性研究[J].草业学报,2016,25(9):189-196.
[8]王卫,杨水平,崔广林,等.青蒿花粉活力及柱头可授性研究[J].西南大学学报(自然科学版),2015,37(2):1-7.
[9]刘玉玲,潘文,张方秋,等.杜鹃红山茶花粉保存及其生活力测定[J].广东林业科技,2010,26(2):1-6.
[10]李元元,曹红星,李新国.3种果壳类型油棕花粉活力及柱头可授性的比较[J].热带作物学报,2018,39(3):459-464.
[11]彭亮,安衍茹,黄涛,等.黄芩花粉活力及柱头可授性分析[J].中国实验方剂学杂志,2018,24(10):304-308.
[12]郭艳平.褐毛铁线莲开花生物学研究[D].哈尔滨:东北林业大学,2018.
[13]武喆,刘霞,张光星.不同温度对胡萝卜花粉活力的影响[J].华北农学报,2010,25(4):116-118.
[14]刘会超,贾文庆.离体萌发法测定垂丝海棠花粉生活力的研究[J].山西农业科学,2007,35(5):39-41.
[15]CARPENTER J L,PLOENSE S E,SNUSTAD D P,et al.Preferential expression of an alpha-tubulin gene of Arabidopsis in pollen[J].Plant Cell,1992,4(5):557-571.
[16]谭健晖.贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响[J].林业科学,2011,47(9):28-32.
[17]张洪伟,段一凡,李稚,等.不同贮藏方法对桂花花粉活力影响的研究[J].南京林业大学学报(自然科学版),2014,38(S1):7-12.
[18]王碧芳,袁德义,刘冬明,等.不同贮藏条件下锥栗花粉生活力研究[J].经济林研究,2014,32(4):73-76.
[19]黄永芳,吴雪辉,何美儿,等.3种油茶植物花粉贮藏及生活力的研究[J].森林与环境学报,2011,31(1):56-59.
[20]蒋京桥,牛红彬,关文灵,等.毛茛铁线莲开花结实生物学特性研究[J].中国园艺文摘,2013,29(1):13-15.
[21]王非,赵舒野,姜思佳,等.不同散粉期胡桃楸离体花粉生活力的测定[J].森林工程,2013,29(3):42-44.
[22]严靖.圆锥铁线莲的繁殖生物学研究[D].南京:南京林业大学,2013.
[23]王欢.绵枣儿开花生物学特性研究[D].哈尔滨:东北林业大学,2017.
[24]孙颖,王阿香,陈士惠,等.野生侧金盏花的花粉活力与柱头可授性[J].草业科学,2014,31(6):1045-1051.
[25]ALCARAZ M L,HORMAZA J I.Optimization of controlled pollination in avocado(Persea americana,Mill.Lauraceae)[J].Scientia Horticulturae,2014,180:79-85.
[26]杨中义,尚嘉琪,张春来,等.地被菊柱头可授性[J].东北林业大学学报,2018,46(3):50-53.
[27]杨芩,万兴权,李东平,等.温度对“杰兔”兔眼蓝莓花粉活力及柱头可授性的影响[J].北方园艺,2017(14):39-43.
[28]古丽江·贾曼拜,古力西拉·沙菩西.不同光照条件对新疆猪牙花花粉生活力及柱头可授性的影响[J].南方农业学报,2013,44(9):1444-1447.
[29]高文杰,王欢,王想,等.野生绵枣儿的花粉活力与柱头可授性[J].东北林业大学学报,2017,45(9):45-48.
[2]王文采,李良千.铁线莲属:新分类系统[J].植物分类学报,2005,43(5):431-488.
[3]钱仁卷,郑坚,胡青荻,等.中国铁线莲属观赏种质资源研究进展[J].中国农学通报,2017,33(21):75-81.
[4]吴荣.观赏铁线莲离体培养研究[D].昆明:西南林业大学,2011.
[5]余伟军.铁线莲遗传多样性、杂交育种及离体快繁研究[D].福州:福建农林大学,2017.
[6]阮丽丽.铁线莲属植物的杂交和扦插研究[C]//张启祥.中国观赏园艺研究进展.北京:中国林业出版社,2014.
[7]苏芸芸,王康才,薛启.不同产地藿香花粉活力与柱头可授性研究[J].草业学报,2016,25(9):189-196.
[8]王卫,杨水平,崔广林,等.青蒿花粉活力及柱头可授性研究[J].西南大学学报(自然科学版),2015,37(2):1-7.
[9]刘玉玲,潘文,张方秋,等.杜鹃红山茶花粉保存及其生活力测定[J].广东林业科技,2010,26(2):1-6.
[10]李元元,曹红星,李新国.3种果壳类型油棕花粉活力及柱头可授性的比较[J].热带作物学报,2018,39(3):459-464.
[11]彭亮,安衍茹,黄涛,等.黄芩花粉活力及柱头可授性分析[J].中国实验方剂学杂志,2018,24(10):304-308.
[12]郭艳平.褐毛铁线莲开花生物学研究[D].哈尔滨:东北林业大学,2018.
[13]武喆,刘霞,张光星.不同温度对胡萝卜花粉活力的影响[J].华北农学报,2010,25(4):116-118.
[14]刘会超,贾文庆.离体萌发法测定垂丝海棠花粉生活力的研究[J].山西农业科学,2007,35(5):39-41.
[15]CARPENTER J L,PLOENSE S E,SNUSTAD D P,et al.Preferential expression of an alpha-tubulin gene of Arabidopsis in pollen[J].Plant Cell,1992,4(5):557-571.
[16]谭健晖.贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响[J].林业科学,2011,47(9):28-32.
[17]张洪伟,段一凡,李稚,等.不同贮藏方法对桂花花粉活力影响的研究[J].南京林业大学学报(自然科学版),2014,38(S1):7-12.
[18]王碧芳,袁德义,刘冬明,等.不同贮藏条件下锥栗花粉生活力研究[J].经济林研究,2014,32(4):73-76.
[19]黄永芳,吴雪辉,何美儿,等.3种油茶植物花粉贮藏及生活力的研究[J].森林与环境学报,2011,31(1):56-59.
[20]蒋京桥,牛红彬,关文灵,等.毛茛铁线莲开花结实生物学特性研究[J].中国园艺文摘,2013,29(1):13-15.
[21]王非,赵舒野,姜思佳,等.不同散粉期胡桃楸离体花粉生活力的测定[J].森林工程,2013,29(3):42-44.
[22]严靖.圆锥铁线莲的繁殖生物学研究[D].南京:南京林业大学,2013.
[23]王欢.绵枣儿开花生物学特性研究[D].哈尔滨:东北林业大学,2017.
[24]孙颖,王阿香,陈士惠,等.野生侧金盏花的花粉活力与柱头可授性[J].草业科学,2014,31(6):1045-1051.
[25]ALCARAZ M L,HORMAZA J I.Optimization of controlled pollination in avocado(Persea americana,Mill.Lauraceae)[J].Scientia Horticulturae,2014,180:79-85.
[26]杨中义,尚嘉琪,张春来,等.地被菊柱头可授性[J].东北林业大学学报,2018,46(3):50-53.
[27]杨芩,万兴权,李东平,等.温度对“杰兔”兔眼蓝莓花粉活力及柱头可授性的影响[J].北方园艺,2017(14):39-43.
[28]古丽江·贾曼拜,古力西拉·沙菩西.不同光照条件对新疆猪牙花花粉生活力及柱头可授性的影响[J].南方农业学报,2013,44(9):1444-1447.
[29]高文杰,王欢,王想,等.野生绵枣儿的花粉活力与柱头可授性[J].东北林业大学学报,2017,45(9):45-48.