高效液相色谱法同时测定白虎加桂枝汤中5个有效指标性成分的含量
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摘要
目的:建立白虎加桂枝汤中芒果苷、新芒果苷、甘草酸、甘草苷、肉桂酸5个有效指标性成分的含量测定方法,为白虎加桂枝汤的颗粒研发奠定基础。方法:采用HPLC-PDA双波长同时测定,Waters x BridgeBEH C18(4.6 mm×250μm,5μm)色谱柱,流动相乙腈-0.01%甲酸水,梯度洗脱,流速1 m L·min-1,柱温24℃,检测波长255 nm、280 nm。结果:内芒果苷、新芒果苷、甘草酸、甘草苷、肉桂酸线性范围分别为66-1 976μg(r=0.9993),70-3 487μg(r=0.999 7),30-913μg(r=0.999 5),35-1 734μg(r=0.999 5),0.6-187μg(r=0.999 8),加样回收率平均范围95.29%-100.17%。结论:建立了白虎加桂枝汤中5个有效指标性成分的含量测定方法,该方法简捷、稳定、准确,可为白虎加桂枝汤及其复方颗粒研制的质量控制提供评价方法。
This study was aimed to develop an HPLC method for simultaneous determination of five active components in Baihu and Guizhi decoction. Simultaneous determination of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin, and cinnamic acid in Baihu and Guizhi decoction were conducted by HPLC-PDA under multiple UV wavelengths. An Waters x Bridge BEH C18 Column(4.6 mm × 250 μm, 5 μm) was used. The mobile phase was acetonitrile-0.01% formic acid solution. The flow rate was 1 m L·min-1. The column temperature was kept at 24°C. The detection wavelength was set at255 nm and 280 nm. The results showed that the linear range of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin,and cinnamic acid was 66-1 976 μg(r = 0.999 3), 70-3487 μg(r = 0.999 7), 30-913 μg(r = 0.999 5), 35-1 734 μg(r =0.999 5), 0.6-187 μg(r = 0.999 8) in 70 min, respectively. The average recovery was between 95.29% and 100.17%. It was concluded that the method was convenient, stable, reliable and accurate for simultaneous determination of the five components in Baihu and Guizhi decoction. It provided an evaluation method for the quality control of Baihu and Guizhi decoction and the research o its granules.
This study was aimed to develop an HPLC method for simultaneous determination of five active components in Baihu and Guizhi decoction. Simultaneous determination of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin, and cinnamic acid in Baihu and Guizhi decoction were conducted by HPLC-PDA under multiple UV wavelengths. An Waters x Bridge BEH C18 Column(4.6 mm × 250 μm, 5 μm) was used. The mobile phase was acetonitrile-0.01% formic acid solution. The flow rate was 1 m L·min-1. The column temperature was kept at 24°C. The detection wavelength was set at255 nm and 280 nm. The results showed that the linear range of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin,and cinnamic acid was 66-1 976 μg(r = 0.999 3), 70-3487 μg(r = 0.999 7), 30-913 μg(r = 0.999 5), 35-1 734 μg(r =0.999 5), 0.6-187 μg(r = 0.999 8) in 70 min, respectively. The average recovery was between 95.29% and 100.17%. It was concluded that the method was convenient, stable, reliable and accurate for simultaneous determination of the five components in Baihu and Guizhi decoction. It provided an evaluation method for the quality control of Baihu and Guizhi decoction and the research o its granules.
引文
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2 范衡.白虎加桂枝汤临床新用体会.实用中医药杂志,2013,29(11):948-949.
3 丁仲华.白虎加桂枝汤加减治疗热痹的体会.中医药学刊,2005,23(8):1489-1490.
4 赵娇.白虎汤中石膏清热作用的成分研究.哈尔滨:黑龙江中医药大学硕士学位论文,2012.
4 夏怡,李祥,陈建伟,等.石膏及白虎汤清热泻火功效的实验研究.现代中药研究与实践,2009,23(2):48-51.
5 王娅,冯芳,孙丽丽.白虎汤配伍谱效关系研究.药学与临床研究,2010,18(3):226-230.
6 马强,李晓晶,顾丽,等.白虎汤不同配伍对甘草酸含量的影响.中华中医药杂志,2011,(9):2049-2051.
7 卢林,吴君金,马强,等.UPLC研究白虎汤不同配伍对芒果苷含量的影响.中国实验方剂学杂志,2011,26(9):35-37.
8 刘敏,赵白云,赵亮,等.炮制前后知母中芒果苷和新芒果苷的含量变化.第二军医大学学报,2006,27(5):528-530.
9 刘海峰.知母药材中芒果苷的制备以及白虎汤中芒果苷的初步研究.成都:四川大学硕士学位论文,2007.
10 陈新阳,毕开顺,易丽昕,等.双波长切换HPLC同时测定桂枝甘草汤中4种成分的含量.中国现代应用药学,2011,28(3):265-267.
11 李春来,李伟东,吴育,等.HPLC同时测定白虎汤中4种有效成分的含量.中国实验方剂学杂志,2012,18(12):78-80.
12 王智民,叶祖光,肖诗鹰,等.对中药配方颗粒发展的几点建议和应用前景分析.中国中药杂志,2004,29(1):5-7.
13 崔景朝,赵自明.中药配方颗粒研究进展(Ⅱ)——中药单煎与合煎对比研究概况.中国实验方剂学杂志,2011,17(4):240-245.
14 李松林,宋景政,徐宏喜.中药配方颗粒研究浅析.中草药,2009,40(S1):1-7.
2 范衡.白虎加桂枝汤临床新用体会.实用中医药杂志,2013,29(11):948-949.
3 丁仲华.白虎加桂枝汤加减治疗热痹的体会.中医药学刊,2005,23(8):1489-1490.
4 赵娇.白虎汤中石膏清热作用的成分研究.哈尔滨:黑龙江中医药大学硕士学位论文,2012.
4 夏怡,李祥,陈建伟,等.石膏及白虎汤清热泻火功效的实验研究.现代中药研究与实践,2009,23(2):48-51.
5 王娅,冯芳,孙丽丽.白虎汤配伍谱效关系研究.药学与临床研究,2010,18(3):226-230.
6 马强,李晓晶,顾丽,等.白虎汤不同配伍对甘草酸含量的影响.中华中医药杂志,2011,(9):2049-2051.
7 卢林,吴君金,马强,等.UPLC研究白虎汤不同配伍对芒果苷含量的影响.中国实验方剂学杂志,2011,26(9):35-37.
8 刘敏,赵白云,赵亮,等.炮制前后知母中芒果苷和新芒果苷的含量变化.第二军医大学学报,2006,27(5):528-530.
9 刘海峰.知母药材中芒果苷的制备以及白虎汤中芒果苷的初步研究.成都:四川大学硕士学位论文,2007.
10 陈新阳,毕开顺,易丽昕,等.双波长切换HPLC同时测定桂枝甘草汤中4种成分的含量.中国现代应用药学,2011,28(3):265-267.
11 李春来,李伟东,吴育,等.HPLC同时测定白虎汤中4种有效成分的含量.中国实验方剂学杂志,2012,18(12):78-80.
12 王智民,叶祖光,肖诗鹰,等.对中药配方颗粒发展的几点建议和应用前景分析.中国中药杂志,2004,29(1):5-7.
13 崔景朝,赵自明.中药配方颗粒研究进展(Ⅱ)——中药单煎与合煎对比研究概况.中国实验方剂学杂志,2011,17(4):240-245.
14 李松林,宋景政,徐宏喜.中药配方颗粒研究浅析.中草药,2009,40(S1):1-7.