白虎加桂枝汤单煎与合煎提取物质量表征特性分析
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摘要
为研究白虎加桂枝汤单煎和合煎提取模式中药学质量表征及药效质量表征的同异性,该实验以相同工艺参数分别制备白虎加桂枝汤单煎和合煎提取物,基于两者HPLC图谱、指标性成分的含量及其提取量比较分析其药学质量表征特性同异性,并基于两者在炎症模型和疼痛模型下的药效实验结果比较分析其药效质量表征特性同异性。研究发现两者HPLC图峰表征未见明显质的差异,但峰面积值表征反映出量的差异,主要表征特性为单煎提取物小于合煎提取物的色谱峰主要来源于知母和桂枝;而单煎提取物大于合煎提取物的色谱峰主要来源于炙甘草;指标性成分中新芒果苷、芒果苷、知母皂苷BⅡ的含量及其提取量为合煎提取物高于单煎提取物,甘草苷、甘草酸的含量及其提取量为合煎提取物低于单煎提取物,肉桂酸的含量及其提取量为合煎提取物与单煎提取物无明显差异。药效实验结果表明两者均在一定程度上显示了抗炎、镇痛作用;但合煎提取物显示了较快速的抗炎药效作用,较快且持续时间较长的镇痛作用。由此可知,白虎加桂枝汤方单煎与合煎具有相同的有效物质基础,且其合煎的模式有利于促进本方中知母和桂枝有效成分的溶出,继而可更有利于其相关疗效的发挥。
To study the differences and similarities in pharmaceutical characterization and pharmacodynamic characterization between the single decoction and merger decoction of Baihu and Guizhi. The same technology parameters were used to prepare Baihu and Guizhi single decoticon and merger decoction extracts,and then the differences and similarities in pharmaceutical characterization were analyzed based on their HPLC fingerprint,content of index components,and the extraction content. The pharmacodynamic differences and similarities were analyzed by inflammatory model and pain model. There was no significant difference in HPLC chromatographic peak,but the peak area value reflected the difference of quantity to some extent. It was found that the peak value of single Rhizoma anemarrhenae and Cassia twig decoction was less than the peak of their merger decoction,but the peak value of single honey-fried Licorice root decoction was greater than the peak of merger decoction. The contents of neomangiferin,mangiferin and timosaponin B Ⅱamong index components as well as extraction content in merger decoction were higher than those in single decoction. The contents of liquiritin and glycyrrhizic acid as well as extraction content in merger decoction were lower than those in single decoction. There was no significant difference in the content of cinnamicacid and its extraction content between merger decoction and single decoction. According to the efficacy experiment,both of them showed significant anti-inflammatory and analgesic effects. However,the merger decoction showed faster anti-inflammation effect,and longer analgesic effect. It can be concluded that the merger decoction and single decoction of Baihu and Guizhi have the same material basis,and the merger decoction is better for the dissolution of the active ingredients in this recipe,and is more beneficial to the therapeutic effect.
To study the differences and similarities in pharmaceutical characterization and pharmacodynamic characterization between the single decoction and merger decoction of Baihu and Guizhi. The same technology parameters were used to prepare Baihu and Guizhi single decoticon and merger decoction extracts,and then the differences and similarities in pharmaceutical characterization were analyzed based on their HPLC fingerprint,content of index components,and the extraction content. The pharmacodynamic differences and similarities were analyzed by inflammatory model and pain model. There was no significant difference in HPLC chromatographic peak,but the peak area value reflected the difference of quantity to some extent. It was found that the peak value of single Rhizoma anemarrhenae and Cassia twig decoction was less than the peak of their merger decoction,but the peak value of single honey-fried Licorice root decoction was greater than the peak of merger decoction. The contents of neomangiferin,mangiferin and timosaponin B Ⅱamong index components as well as extraction content in merger decoction were higher than those in single decoction. The contents of liquiritin and glycyrrhizic acid as well as extraction content in merger decoction were lower than those in single decoction. There was no significant difference in the content of cinnamicacid and its extraction content between merger decoction and single decoction. According to the efficacy experiment,both of them showed significant anti-inflammatory and analgesic effects. However,the merger decoction showed faster anti-inflammation effect,and longer analgesic effect. It can be concluded that the merger decoction and single decoction of Baihu and Guizhi have the same material basis,and the merger decoction is better for the dissolution of the active ingredients in this recipe,and is more beneficial to the therapeutic effect.
引文
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[3]马学玉.加味白虎加桂枝汤治疗类风湿关节炎湿热痹阻证临床研究[J].中医学报,2016,31(10):1573.
[4]陈欢,巨少华,魏江平,等.白虎加桂枝汤对热痹模型大鼠特征性甲基化基因表达的影响[J].中国中药杂志,2017,42(2):332.
[5]巨少华.湿热环境对RA模型DNA甲基化影响及基于炎症反应的白虎加桂枝汤初步验证[D].成都:成都中医药大学,2015.
[6]王娅,冯芳,孙丽丽.白虎汤配伍谱效关系研究[J].药学与临床研究,2010,18(3):226.
[7]彭平,王梦婕,李晓盈,等.高效液相色谱法同时测定白虎加桂枝汤中5个有效指标性成分的含量[J].世界科学技术——中医药现代化,2017,19(8):1391.
[8]中国药典.一部[S].2015:213.