超快速液相色谱-串联质谱法测定血液中的右美托咪定
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摘要
建立采用超快速液相色谱-串联质谱法快速检测人体血液中右美托咪定成分的定性定量分析方法。采用乙腈沉淀蛋白法对血液进行前处理,LC-MS/MS多反应监测模式采集,以右美托咪定的两对离子对和保留时间定性,外标-标准曲线法进行定量分析。该方法检测血液中右美托咪定成分在1ng/m L～100ng/mL浓度范围内呈良好的线性(r=0.9984)。方法最低检出限是0.2ng/mL,定量限是1ng/m L,日内和日间精密度均在6%以下。该方法简单、快速、准确,有较好的重现性和较高的回收率,适用于实际案件中血液中右美托咪定的快速定性定量分析。
引文
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[2]舒成仁,黄露,葛苗苗,等.盐酸右美托咪定注射液在健康人体的药动学[J].医药导报,2011(12):1555-1558.
[3]王瑞花,刘瑞,栾玉静,等.固相萃取-气相色谱-质谱法检验人血浆中的右美托咪定[J].中国法医学杂志, 2017(5):497-499.
[4]Hui Y H,Marsh K C,MenacherryS.Analyticalmethoddevelopmentforthesimultaneousquantitationofdexmedetomidine and three potential metabolites in plasma[J].Journal of Chromatography A,1997(1-2):281.
[5]高存强,韦艳丽,马仕珉,等.HPLC法测定盐酸右美托咪定及注射剂中的R-异构体[J].中国医药导报,2010(18):63-64.
[6]李鸿雁,张顺利,刘珍仁,等.HPLC法测定盐酸右美托咪定有关物质[J].药物分析杂志,2015(12):2193-2198.
[7]LiuHC,SunW,WangCY,etal.Determinationofdexmedetomidineinchildren’splasmabyultra-performance liquid chromatography tandem mass spectrometryand application to pharmacokinetic study[J].Journal ofChromatography B,2016,1023-1024:30-35.
[8]Cui Z,Chow D S,Wu L,et al. High performance liquidchromatography-tandemmassspectrometricassayofdexmedetomidine in plasma, urine and amniotic fluidsamples for pregnant ewe model[J].Journal of ChromatographyB,2014,961:13-19.
[9]孙会会,仲建军,翟晚枫.LC-MS/MS测定血液中利眠宁成分[J].刑事技术,2016(5):376-379.
[10]孙会会,仲建军.LC-MS/MS同时分析血液中8种苯二氮卓类药物[J].化学工程师,2016(5):30-32.
[11]宋蕊,武继锋,刘海燕,等.HPLC-MS/MS法快速检测人血中钩吻毒素[J].中国刑警学院学报,2017(2):103-106.