调中健胃丸质量标准研究
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摘要
目的探索调中健胃丸的质量标准。方法采用薄层色谱法对调中健胃丸中丹参、白术、半夏进行定性鉴别;采用高效液相色谱法测定处方中芍药有效成分芍药苷的含量。结果薄层色谱中在与对照药材色谱相应的位置上均可检出丹参、白术、半夏相应的斑点;芍药苷在0. 12～0. 6μg内呈良好的线性关系,r=0. 9991,平均回收率为98. 66%,RSD为0. 70%。结论该方法操作简便,结果准确,重复性良好,可作检测调中健胃丸质量的控制标准。
Objective: To investigate the quality standard of Regulating Spleen-Stomach and Strengthening Stomach Pill( Tiaozhong Jianwei Pill). Method: TLC was used to identify Salvia miltiorrhiza,Atractylodes macrocephala and Pinellia ternata in Tiaozhong Jianwei Pills. The content of paeoniflorin in the prescription was determined by HPLC. Result: The corresponding spots of Salvia miltiorrhiza,Atractylodes macrocephala and Pinellia ternata could be detected by TLC at the corresponding positions of the control herbs. Paeoniflorin showed a good linear relationship within 0. 12-0. 6 ug,r = 0. 9991,with an average recovery of 98. 66% and RSD of 0. 70%. Conclusion: The method is simple,accurate and reproducible. It can be used as a quality control standard for Regulating Spleen-Stomach and Strengthening Stomach Pill( Tiaozhong Jianwei Pill).
Objective: To investigate the quality standard of Regulating Spleen-Stomach and Strengthening Stomach Pill( Tiaozhong Jianwei Pill). Method: TLC was used to identify Salvia miltiorrhiza,Atractylodes macrocephala and Pinellia ternata in Tiaozhong Jianwei Pills. The content of paeoniflorin in the prescription was determined by HPLC. Result: The corresponding spots of Salvia miltiorrhiza,Atractylodes macrocephala and Pinellia ternata could be detected by TLC at the corresponding positions of the control herbs. Paeoniflorin showed a good linear relationship within 0. 12-0. 6 ug,r = 0. 9991,with an average recovery of 98. 66% and RSD of 0. 70%. Conclusion: The method is simple,accurate and reproducible. It can be used as a quality control standard for Regulating Spleen-Stomach and Strengthening Stomach Pill( Tiaozhong Jianwei Pill).
引文
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[10]黄卫娟,何秀云,刘杰,等. HPLC法测定八珍丸(浓缩丸)中芍药苷的含量[J].中国药房,2016,27(15):2126.
[11]杨慈海,范常龙.高效液相色谱法测定斑秃丸中芍药苷的含量[J].海峡药学,2014,26(3):45.
[12]于红艳,张宾.反相高效液相色谱法测定降酶丸中芍药苷的含量[J].中医学报,2014,29(4):546-547.
[13]李喜香,刘效栓,毕映燕,等.补脑软胶囊质量标准的研究[J].中成药,2016,38(2):321.
[14]杨仕林,王雨.反相高效液相色谱法同时测定正柴胡饮合剂中橙皮苷和芍药苷含量[J].中国药业,2015,24(21):121.
[15]毕映燕,李秀文,刘效栓,等. HPLC法测定复方赤芍颗粒中芍药苷含量[J].中国中医药信息杂志,2014,21(4):78.
[16]尤立华,赵希贤.氧化铝对正天丸中芍药苷含量测定结果影响的探析[J].首都医药,2014,4(7):53-54.
[2]孙榕,栾永福,李晓宇,等.芍药苷抗神经元损伤药理作用研究进展[J].山东中医药大学报,2012,36(5):454-456.
[3]李春香,丁里玉,丁芳,等.白芍养血柔肝止痛作用与其配伍及芍药苷含量的相关性研究[J].中药新药与临床药理,2011,22(1):54-56.
[4]李娉婷,陈子骄,贾薇,等.中药胶囊剂中芍药苷含量测定方法优化[J].时珍国医国药,2014,25(10):2357-2359.
[5]林爱琴,蒋建霞. HPLC测定健胃消炎颗粒中芍药苷的含量[J].中国医药导报,2010,7(3):54.
[6]严亚锋,史亚军.舒心通络丸质量标准研究[J].陕西中医药大学学报,2016,39(2):93-97.
[7]陈亚龙,李昭,曹林林,等.益视口服液质量标准提高研究[J].现代中医药,2017,37(2):76-80.
[8]胡轶娟,浦锦宝,王爱贵,等.高效液相法测定白芍药材中白芍总苷的含量[J].医学研究杂志,2008,37(8):104.
[9]覃英镨,莫武明. HPLC法测定小儿泻痢片中芍药苷的含量[J].中国医药科学,2011,1(14):134.
[10]黄卫娟,何秀云,刘杰,等. HPLC法测定八珍丸(浓缩丸)中芍药苷的含量[J].中国药房,2016,27(15):2126.
[11]杨慈海,范常龙.高效液相色谱法测定斑秃丸中芍药苷的含量[J].海峡药学,2014,26(3):45.
[12]于红艳,张宾.反相高效液相色谱法测定降酶丸中芍药苷的含量[J].中医学报,2014,29(4):546-547.
[13]李喜香,刘效栓,毕映燕,等.补脑软胶囊质量标准的研究[J].中成药,2016,38(2):321.
[14]杨仕林,王雨.反相高效液相色谱法同时测定正柴胡饮合剂中橙皮苷和芍药苷含量[J].中国药业,2015,24(21):121.
[15]毕映燕,李秀文,刘效栓,等. HPLC法测定复方赤芍颗粒中芍药苷含量[J].中国中医药信息杂志,2014,21(4):78.
[16]尤立华,赵希贤.氧化铝对正天丸中芍药苷含量测定结果影响的探析[J].首都医药,2014,4(7):53-54.