何首乌标准饮片的HPLC特征图谱分析
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摘要
目的:建立何首乌标准饮片的HPLC特征图谱,并与何首乌原形饮片、原料药材、对照药材的HPLC特征图谱进行对比分析。方法:采用HPLC,Waters BEH-C_(18)色谱柱(4. 6 mm×250 mm,5μm),流动相甲醇(A)-0. 1%磷酸水溶液(B)梯度洗脱(0～20 min,15%～30%A; 20～35 min,30%～40%A; 35～55 min,40%～75%A; 55～75 min,75%～100%A),柱温30℃,检测波长270 nm,流速0. 8 mL·min~(-1),进样量10μL;对何首乌标准饮片及其原形饮片、原料药材、对照药材的HPLC特征图谱进行相似度分析和聚类分析。结果:构建了由7个色谱峰组成的何首乌标准饮片HPLC特征图谱;何首乌标准饮片与原形饮片特征图谱的相似度达0. 999,优于原料药材、对照药材与原形饮片特征图谱的相似度;标准饮片与原形饮片的图谱在色谱峰个数上无明显差异,而对照药材与原形饮片图谱在峰个数上有明显差异;并且标准饮片与原形饮片、对照药材大体上聚为一类,而原料药材大体上聚为一类。结论:相比较原料药材和对照药材,所建立的何首乌标准饮片HPLC特征图谱能更好地反映原形饮片的内在质量,可用于何首乌饮片的质量控制。
Objective: To establish HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix,and compare with the HPLC characteristic chromatograms of original pieces,raw materials and control materials of Polygoni Multiflori Radix. Method: HPLC analysis was carried out on a Waters BEH-C_(18) column( 4. 6 mm × 250 mm,5 μm) with the mobile phase of methanol( A)-0. 1% phosphoric acid( B) for gradient elution( 0-20 min,15%-30% A; 20-35 min,30%-40% A; 35-55 min,40%-75% A; 55-75 min,75%-100% A) at a flow rate of 0. 8 mL·min~(-1). The detection wavelength was set at 270 nm and the column temperature was maintained at 30 ℃. The injection volume was 10 μL. Moreover,similarity and cluster analysis of HPLC characteristic chromatograms of four samples of Polygoni Multiflori Radix were performed. Result: HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix composed of seven peaks was established. The similarity was 0. 999 between characteristic chromatograms of standard pieces and original pieces,which was better than similarities among characteristic chromatograms of raw materials,control materials and original pieces. There was no obvious difference on number of peaks between characteristic chromatograms of standard pieces and original pieces,while an obvious difference on number of peaks between chromatograms of control materials and original pieces was found. In addition,standard pieces,original pieces and control materials could generally gather into one class,while raw materials could generally gather into another class. Conclusion:Compared with the raw materials and control materials,the established HPLC characteristic chromatogram of standard pieces can better reflect the internal quality of original pieces,which can be used for the quality control of decoction pieces of Polygoni Multiflori Radix.
Objective: To establish HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix,and compare with the HPLC characteristic chromatograms of original pieces,raw materials and control materials of Polygoni Multiflori Radix. Method: HPLC analysis was carried out on a Waters BEH-C_(18) column( 4. 6 mm × 250 mm,5 μm) with the mobile phase of methanol( A)-0. 1% phosphoric acid( B) for gradient elution( 0-20 min,15%-30% A; 20-35 min,30%-40% A; 35-55 min,40%-75% A; 55-75 min,75%-100% A) at a flow rate of 0. 8 mL·min~(-1). The detection wavelength was set at 270 nm and the column temperature was maintained at 30 ℃. The injection volume was 10 μL. Moreover,similarity and cluster analysis of HPLC characteristic chromatograms of four samples of Polygoni Multiflori Radix were performed. Result: HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix composed of seven peaks was established. The similarity was 0. 999 between characteristic chromatograms of standard pieces and original pieces,which was better than similarities among characteristic chromatograms of raw materials,control materials and original pieces. There was no obvious difference on number of peaks between characteristic chromatograms of standard pieces and original pieces,while an obvious difference on number of peaks between chromatograms of control materials and original pieces was found. In addition,standard pieces,original pieces and control materials could generally gather into one class,while raw materials could generally gather into another class. Conclusion:Compared with the raw materials and control materials,the established HPLC characteristic chromatogram of standard pieces can better reflect the internal quality of original pieces,which can be used for the quality control of decoction pieces of Polygoni Multiflori Radix.
引文
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[12]苏晓,冯伟红,李娆娆,等.不同产地加工方法对何首乌饮片质量的影响[J].中国实验方剂学杂志,2017,23(13):18-23.
[13]卢吉燕,龙明.不同炮制工艺对何首乌有效成分含量的影响[J].河南中医,2015,35(12):3190-3192.
[14]王焱.高效液相色谱法同时测定制何首乌中多种活性成分[J].亚太传统医药,2016,12(19):45-47.
[15]郑英,李玮,赵贵,等.基于过程控制的何首乌产地加工与炮制一体化方法分析[J].中国实验方剂学杂志,2018,24(15):29-35.
[16]朱培芳,赵紫伟,赵荣华,等.高效液相色谱法测定12个市售地何首乌中有效成分的含量[J].中华中医药杂志,2014,29(11):3605-3607.
[17]钟国跃,张倩,瞿显友. HPLC测定不同生长年限及采收期何首乌中二苯乙烯苷和蒽醌类成分的含量[J].中国中药杂志,2010,35(10):1221-1225.
[2]吴菊芳,黄少君.中药炮制对中药饮片的化学成分及疗效的影响[J].中国现代药物应用,2018,12(12):207-208.
[3]李丽,刘颖,肖永庆.中药标准饮片作为标准物质应用若干问题探讨[J].北京中医药大学学报,2015,38(6):411-412,419.
[4]肖永庆,李丽,刘颖.加快中药标准饮片作为标准物质的研究[J].中国中药杂志,2014,39(13):2428-2431.
[5]王新杰,王英姿,肖永庆,等.川楝子与炒川楝子标准饮片的均匀化工艺研究[J].现代中药研究与实践,2018,32(6):53-57.
[6]高笑笑,李霞,苗静,等.天麻炮制及其标准饮片的均匀化工艺研究[J].天津中医药大学学报,2018,37(6):500-505.
[7]翟文泽,任晶,孟江,等.制何首乌标准饮片的均匀化工艺研究[J].广东药科大学学报,2017,33(2):172-176.
[8]李水清,孙萌,梁生旺,等.焦槟榔标准饮片均匀化方法及质量控制的研究[J].中药材,2018,41(7):1699-1702.
[9]窦珮丹,李克宁,龙琴,等.槟榔标准饮片均匀化方法研究[J].亚太传统医药,2017,13(9):15-18.
[10]任晶,翟文泽,梁生旺,等.何首乌标准饮片均匀化方法研究[J].亚太传统医药,2017,13(6):16-19.
[11]王芳,于欢,李风琴,等.枳实制白术的炮制工艺优选及HPLC特征图谱分析[J].中国实验方剂学杂志,2018,24(17):6-13.
[12]苏晓,冯伟红,李娆娆,等.不同产地加工方法对何首乌饮片质量的影响[J].中国实验方剂学杂志,2017,23(13):18-23.
[13]卢吉燕,龙明.不同炮制工艺对何首乌有效成分含量的影响[J].河南中医,2015,35(12):3190-3192.
[14]王焱.高效液相色谱法同时测定制何首乌中多种活性成分[J].亚太传统医药,2016,12(19):45-47.
[15]郑英,李玮,赵贵,等.基于过程控制的何首乌产地加工与炮制一体化方法分析[J].中国实验方剂学杂志,2018,24(15):29-35.
[16]朱培芳,赵紫伟,赵荣华,等.高效液相色谱法测定12个市售地何首乌中有效成分的含量[J].中华中医药杂志,2014,29(11):3605-3607.
[17]钟国跃,张倩,瞿显友. HPLC测定不同生长年限及采收期何首乌中二苯乙烯苷和蒽醌类成分的含量[J].中国中药杂志,2010,35(10):1221-1225.