黄精多糖对强迫运动大鼠脾脏免疫功能的影响
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摘要
为探讨黄精多糖对大鼠脾脏免疫功能的影响,将36只试验大鼠中6只较懒惰大鼠淘汰,剩余30只按体重随机分为安静组、运动组和运动给药组,每组10只,试验前对3组大鼠进行体重测定及一次力竭跑台练习,3组大鼠体重、力竭运动时间均无显著差异。试验时运动给药组大鼠灌服剂量为100g·(kg·d)~(-1)黄精多糖溶液,其他2组灌服相应体积的生理盐水。运动训练组、运动给药组进行为期8周递增负荷训练及1周力竭训练,测定各组大鼠体重、脾脏指数、T淋巴细胞增殖能力、B淋巴细胞增殖能力、T淋巴细胞亚群、巨噬细胞吞噬能力。结果表明:与安静组相比,运动组体重、脾脏重、脾脏指数降低,脾脏中T淋巴细胞增殖、B淋巴细胞增殖、巨噬细胞吞噬能力、CD_3~+、CD_4~+、CD_8~+、CD_4~+/CD_8~+降低,运动给药组体重、脾脏重、巨噬细胞吞噬能力降低,运动给药组T淋巴细胞增殖、B淋巴细胞增殖、CD_3~+、CD_4~+、CD_8~+、CD_4~+/CD_8~+无显著差异。与运动组相比,运动给药组体重、脾脏重、脾脏指数升高,脾脏中T淋巴细胞增殖、B淋巴细胞增殖、巨噬细胞吞噬能力、CD_3~+、CD_4~+、CD_8~+、CD_4~+/CD_8~+升高。这一研究提示黄精多糖能显著缓解强迫运动引起的脾脏免疫功能低下,恢复脾脏免疫功能接近正常水平。
This paper aims to study the effect of polygonatum polysaccharide on spleen immune function of rats by establishing a model of exercise immunosuppression.Of 36 experimental rats,6lazy rats were eliminated,and the remaining 30 rats were randomly divided into sedentary group,exercise group,and dosage-given plus traing group,with 10 rats in each group according to their body weight.As a result,there was no significant difference in body mass and exhaustive exercise time among the three groups.In the experiment,the mice in the exercise administration group were given 100g·(kg·d)~(-1) yellow essence polysaccharide solution,and the other two groups were given the corresponding volume of normal saline according to the quality.The exercise group and the dosage-given plus traing group received 8weeks incremental load training and 1 week exhaustive training.The body weight,spleen index and proliferation of T lymphocytes were measured in each group.The proliferation of B lymphocytes and the phagocytosis of macrophages were measured.The results showed that compared with the sedentary group,the body mass,spleen weight,spleen index,T lymphocyte proliferation and B lymphocyte proliferation,macrophage phagocytic capacity,CD_3~+,CD_4~+,CD_8~+,CD_4~+/CD_8~+,body weight and spleen weight in the exercise group were decreased.There was no significant difference in T lymphocyte proliferation and B lymphocyte proliferation CD_3,CD_4,CD_8,CD_4/CD_8 in the dosage-given plus traing group.Compared with exercise group,the body weight,spleen index,proliferation of T lymphocytes and ratio of T lymphocytes in the spleen and CD_3~+,CD_4~+,CD_8~+,CD_4~+/CD_8~+ were increased in the dosage-given plus training group.Polygonatum polysaccharide significantly alleviated the spleen immune function induced by forced exercise and recovered the spleen immune function close to the normal level.
This paper aims to study the effect of polygonatum polysaccharide on spleen immune function of rats by establishing a model of exercise immunosuppression.Of 36 experimental rats,6lazy rats were eliminated,and the remaining 30 rats were randomly divided into sedentary group,exercise group,and dosage-given plus traing group,with 10 rats in each group according to their body weight.As a result,there was no significant difference in body mass and exhaustive exercise time among the three groups.In the experiment,the mice in the exercise administration group were given 100g·(kg·d)~(-1) yellow essence polysaccharide solution,and the other two groups were given the corresponding volume of normal saline according to the quality.The exercise group and the dosage-given plus traing group received 8weeks incremental load training and 1 week exhaustive training.The body weight,spleen index and proliferation of T lymphocytes were measured in each group.The proliferation of B lymphocytes and the phagocytosis of macrophages were measured.The results showed that compared with the sedentary group,the body mass,spleen weight,spleen index,T lymphocyte proliferation and B lymphocyte proliferation,macrophage phagocytic capacity,CD_3~+,CD_4~+,CD_8~+,CD_4~+/CD_8~+,body weight and spleen weight in the exercise group were decreased.There was no significant difference in T lymphocyte proliferation and B lymphocyte proliferation CD_3,CD_4,CD_8,CD_4/CD_8 in the dosage-given plus traing group.Compared with exercise group,the body weight,spleen index,proliferation of T lymphocytes and ratio of T lymphocytes in the spleen and CD_3~+,CD_4~+,CD_8~+,CD_4~+/CD_8~+ were increased in the dosage-given plus training group.Polygonatum polysaccharide significantly alleviated the spleen immune function induced by forced exercise and recovered the spleen immune function close to the normal level.
引文
[1]和卫宾.罗汉果叶黄酮对力竭性运动大鼠免疫功能的影响[D].桂林:广西师范大学,2013.
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[3]韩春杨,杨明川,杨孜生,等.黄精多糖的提取及其对CCl4致大鼠肝损伤的保护作用[J].浙江农业学报,2018,30(4):537-547.
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[5]李超彦,周媛媛,王福青.黄精多糖联合低剂量顺铂对小鼠H22肝癌移植瘤生长的抑制及其抗氧化损伤作用[J].中国老年学杂志,2016,36(5):1038-1040.
[6]赵宏丽,许燕,赵红岩,等.黄精多糖对2型糖尿病大鼠SREBP-1c和SCD-1蛋白表达的影响[J].中药药理与临床,2015,31(1):106-109.
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[9]王雪芹,郝选明.长期递增负荷运动对大鼠脾淋巴细胞增殖活性、凋亡及凋亡相关基因表达的影响[J].中国体育科技,2013,49(6):100-104.
[10]田雪文,刘倩,黄超,等.蛹虫草对过度运动大鼠T淋巴细胞免疫抑制的调节研究[J].山东体育学院学报,2013,29(3):41-45.
[11]蔡国梁,郭霞,汪宇峰,等.不同负荷游泳运动对大鼠部分免疫指标的影响[J].哈尔滨体育学院学报,2017,35(2):1-5,11.
[12]华岩,杨影云,叶绍凡,等.牡蛎提取液对长期训练小鼠免疫功能的影响[J].中国老年学杂志,2012,32(19):4206-4208.
[13]MORGADO J M,RAMA L,SILVA I,et al.Cytokine production by monocytes,neutrophils,and dendritic cells is hampered by long-term intensive training in elite swimmers[J].Eur J Appl Physiol,2012,112(2):471-482.
[14]MURAKAMI S,KURIHARA S,TITCHENAL C A,et al.Suppression of exercise-induced neutrophilia and lymphopenia in athletes by cystine/theanine intake:a randomized,double-blind,placebo-controlled trial[J].J Int Soc Sports Nutr,2010,7(1):23.
[15]王文迪.黄精多糖对一次大强度运动后人体外周血T淋巴细胞免疫功能的影响[D].成都:成都体育学院,2012.
[16]叶绍凡.黄精多糖对力竭运动小鼠胸腺胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞吞噬功能的影响[J].基因组学与应用生物学,2015,34(1):60-65.
[17]华岩,邓武装,王春亮,等.牡蛎多糖对力竭运动小鼠血免疫球蛋白、T淋巴细胞亚群、自然杀伤细胞、自然杀伤T淋巴细胞的影响[J].中国康复医学杂志,2014,29(6):571-573.
[18]肖卫华,陈佩杰,王茹,等.过度训练及补充二联甲苯或谷氨酰胺对大鼠腹膜巨噬细胞活性氧和诱导型一氧化氮合酶的影响[J].体育科学,2011,31(2):49-54.
[19]肖卫华,陈佩杰.IGF-1和MGF在过度训练抑制大鼠腹膜巨噬细胞吞噬功能中的作用研究[J].体育科学,2011,31(10):67-71.
[2]LIU D,WANG R,GRANT A R,et al.Immune adaptation to chronic intense exercise training:new microarray evidence[J].BMC Genomics,2017,18(1):29.
[3]韩春杨,杨明川,杨孜生,等.黄精多糖的提取及其对CCl4致大鼠肝损伤的保护作用[J].浙江农业学报,2018,30(4):537-547.
[4]邓旭坤,段欢,刘钊,等.黄精多糖对环磷酰胺诱导小鼠免疫抑制的影响[J].中南民族大学学报(自然科学版),2018,37(2):49-53.
[5]李超彦,周媛媛,王福青.黄精多糖联合低剂量顺铂对小鼠H22肝癌移植瘤生长的抑制及其抗氧化损伤作用[J].中国老年学杂志,2016,36(5):1038-1040.
[6]赵宏丽,许燕,赵红岩,等.黄精多糖对2型糖尿病大鼠SREBP-1c和SCD-1蛋白表达的影响[J].中药药理与临床,2015,31(1):106-109.
[7]BEDFORD T G,TIPTON C M,WILSON N C,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].J Appl Physiol Respir Environ Exerc Physiol,1979,47(6):1278-1283.
[8]王茹,陈佩杰.运动诱导的TH1/TH2淋巴细胞亚群失衡机制研究进展[J].中国运动医学杂志,2009,28(1):112-117.
[9]王雪芹,郝选明.长期递增负荷运动对大鼠脾淋巴细胞增殖活性、凋亡及凋亡相关基因表达的影响[J].中国体育科技,2013,49(6):100-104.
[10]田雪文,刘倩,黄超,等.蛹虫草对过度运动大鼠T淋巴细胞免疫抑制的调节研究[J].山东体育学院学报,2013,29(3):41-45.
[11]蔡国梁,郭霞,汪宇峰,等.不同负荷游泳运动对大鼠部分免疫指标的影响[J].哈尔滨体育学院学报,2017,35(2):1-5,11.
[12]华岩,杨影云,叶绍凡,等.牡蛎提取液对长期训练小鼠免疫功能的影响[J].中国老年学杂志,2012,32(19):4206-4208.
[13]MORGADO J M,RAMA L,SILVA I,et al.Cytokine production by monocytes,neutrophils,and dendritic cells is hampered by long-term intensive training in elite swimmers[J].Eur J Appl Physiol,2012,112(2):471-482.
[14]MURAKAMI S,KURIHARA S,TITCHENAL C A,et al.Suppression of exercise-induced neutrophilia and lymphopenia in athletes by cystine/theanine intake:a randomized,double-blind,placebo-controlled trial[J].J Int Soc Sports Nutr,2010,7(1):23.
[15]王文迪.黄精多糖对一次大强度运动后人体外周血T淋巴细胞免疫功能的影响[D].成都:成都体育学院,2012.
[16]叶绍凡.黄精多糖对力竭运动小鼠胸腺胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞吞噬功能的影响[J].基因组学与应用生物学,2015,34(1):60-65.
[17]华岩,邓武装,王春亮,等.牡蛎多糖对力竭运动小鼠血免疫球蛋白、T淋巴细胞亚群、自然杀伤细胞、自然杀伤T淋巴细胞的影响[J].中国康复医学杂志,2014,29(6):571-573.
[18]肖卫华,陈佩杰,王茹,等.过度训练及补充二联甲苯或谷氨酰胺对大鼠腹膜巨噬细胞活性氧和诱导型一氧化氮合酶的影响[J].体育科学,2011,31(2):49-54.
[19]肖卫华,陈佩杰.IGF-1和MGF在过度训练抑制大鼠腹膜巨噬细胞吞噬功能中的作用研究[J].体育科学,2011,31(10):67-71.