河北地区中国荷斯坦牛DGAT1基因多态性与泌乳性状遗传效应分析
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摘要
本研究以河北地区870头中国荷斯坦牛为试验材料,采用PCR-RFLP法对DGAT1基因K232A位点进行了遗传多态性分析,并利用混合动物模型分析了DGAT1基因K232A位点突变对乳脂率、乳脂量、乳蛋白率、乳蛋白量、305d产奶量5个泌乳性状的影响。结果共检测到KK、KA和AA 3种基因型,基因频率分别为0.0402、0.9276和0.0321;等位基因K和A的频率分别为0.5040和0.4960;该碱基突变对乳脂率的影响达到极显著水平(P<0.01),对30d产奶量的影响达到显著水平(P<0.05),对乳脂量、乳蛋白率和乳蛋白量的影响不显著(P>0.05);多重比较结果表明,KK基因型的乳脂率极显著高于KA和AA基因型(P<0.01);KA和AA基因型的305d产奶量显著高于KK基因型(P<0.05);AA和KA基因型的泌乳性状无显著差异(P>0.05)。结果显示,DGAT1基因突变对河北荷斯坦奶牛泌乳性状有较大的遗传效应,可用于泌乳性状的分子标记辅助选择。
In this study, the polymorphism in K232 A locus of DGAT1 gene were investigated by PCR-RFLP in a population of 870 Chinese Holstein cows in Hebei province. The effects of K232 A mutation of DGAT1 gene on milk fat percentage, milk fat content, milk protein rate, milk protein content and 305-day milk yield were analyzed by mixed animal model. Three kinds of genotypes were found and the genotypic frequencies of KK, KA and AA were0.0402, 0. 9276 and 0.0321, respectively. Additionally, the gene frequencies of K and A were 0.5040 and 04960,respectively. Significant association was observed between the K232 A site and milk fat rate(P<0.01), 305 days milk yield(P<0.05). Duncan's multiple-range test showed that the cows with KK genotype had higher milk fat rate than those with KA and AA genotypes(P<0.01), while had lower 305-day milk yield(P<0.05). No significant difference were observed in lactation traits between AA and KA genotypes(P>0.05). The results suggested that DGAT1 gene has a great effect on milk production traits and could be a useful genetic marker in Chinese Holstein breeding.
In this study, the polymorphism in K232 A locus of DGAT1 gene were investigated by PCR-RFLP in a population of 870 Chinese Holstein cows in Hebei province. The effects of K232 A mutation of DGAT1 gene on milk fat percentage, milk fat content, milk protein rate, milk protein content and 305-day milk yield were analyzed by mixed animal model. Three kinds of genotypes were found and the genotypic frequencies of KK, KA and AA were0.0402, 0. 9276 and 0.0321, respectively. Additionally, the gene frequencies of K and A were 0.5040 and 04960,respectively. Significant association was observed between the K232 A site and milk fat rate(P<0.01), 305 days milk yield(P<0.05). Duncan's multiple-range test showed that the cows with KK genotype had higher milk fat rate than those with KA and AA genotypes(P<0.01), while had lower 305-day milk yield(P<0.05). No significant difference were observed in lactation traits between AA and KA genotypes(P>0.05). The results suggested that DGAT1 gene has a great effect on milk production traits and could be a useful genetic marker in Chinese Holstein breeding.
引文
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[18]卢振峰,刘莉莉,赵国丽,等.三河牛DGAT1基因K232A位点与产奶性状的关联分析[J].中国奶牛,2014,3:13-16.
[19]周利华,邓政,陈从英,等.DGAT1基因K232A突变位点在3个中国荷斯坦母牛群体中的基因型频率分布及其与产奶量的相关性[J].基因组学与应用生物学,2009,28(6):1087-1091.
[20]张晓东.奶牛DGAT1基因SNPs分析及其与产奶性能关系的研究[D].合肥:安徽农业大学,2009.
[21]徐秀容.DGAT1和DGAT2基因多态性与牛部分经济性状相关性的研究[D].杨凌:西北农林科技大学,2004.
[22]李峥.荷斯坦奶牛DGAT1等3个基因多态与泌乳性状的关联分析[D].大庆:黑龙江八一农垦大学,2016.
[23]殷骥,马海涛,赵国丽,等.中国荷斯坦牛DGAT1基因第8外显子对产奶性状的遗传效应分析[J].黑龙江畜牧兽医,2013,3:37-40.
[24]毛永江,陈仁金,陈莹,等.南方地区中国荷斯坦牛DGAT1基因多态性与泌乳性状的关联分析[J].中国农业科学,2010,43(14):2990-2995.
[25]马彦男,贺鹏迦,马永生,等.DGAT-1基因K232A突变位点对甘肃地区中国荷斯坦牛泌乳性状的影响[J].四川动物,2013,32(6):835-840.
[26]张驭纬,谭志东,付贤圣,等.贵州荷斯坦奶牛甘油二酯酰基转移酶基因多态性及其与产奶性状的关联分析[J].中国畜牧兽医,2013,40(12):56-61.
[27]娄佑武,吴志勇,王荣民,等.吉安西杂母牛DGAT1基因K232A遗传多态性及其对乳成分影响的分析[J].中国牛业科学,2015,41(3):18-21.
[28]邹荣婕.中国荷斯坦奶牛DGAT1、LOX-1基因多态性与产奶性状之间的关系研究[D].杨凌:西北农林科技大学,2007.
[29]巴塔拉伊.奶牛和水牛的MAP4K4和DGAT1基因对产奶性能和体细胞评分的影响与机理的研究[D].武汉:华中农业大学,2017.
[30]Maxa J,Neuditschko M,Russ I,et al.Genome-wide association mapping of milk production traits in Braunvieh cattle[J].Journal of Dairy Science,2012,95(9):5357-5364.
[31]Nayeri S,Sargolzaei M,Aboismail M K,et al.Genome-wide association for milk production and female fertility traits in Canadian dairy Holstein cattle[J].Bmc Genetics,2016,17(1):75.
[32]Bovenhuis H,Visker M H,Van Valenberg H J,et al.Effects of the DGAT1 polymorphism on test-day milk production traits throughout lactation[J].Journal of Dairy Science,2015,98(9):6572-6582.
[33]BartoňL,Bure?D,Kott T,et al.Associations of polymorphisms in bovine DGAT1,FABP4,FASN,and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls[J].Meat Science,2016,114:18-23.
[34]Bovenhuis H,Visker M H,Poulsen N A,et al.Effects of the diacylglycerol o-acyltransferase 1(DGAT1)K232A polymorphism on fatty acid,protein,and mineral composition of dairy cattle milk[J].Journal of Dairy Science,2016,99(4):3113-3123.
[35]Vanbergue E,Peyraud J L,Guinard-Flament J,et al.Effects of DGAT1 K232A polymorphism and milking frequency on milk composition and spontaneous lipolysis in dairy cows[J].Journal of Dairy Science,2016,99(7):5739-5749.
[36]Tomka J,Va?íkováK,OravcováM,et al.Effects of polymorphisms in DGAT1 and LEP genes on milk traits in Holstein primiparous cows[J].Mljekarstvo,2016,66(2):122-128.
[2]Cases S,Smith S J,Zheng Y W,et al.Identification of a gene encoding an Acyl CoA:Diacylglycerol acyltransferase,a key enzyme in triacylglycerol synthesis[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(22):13018-13023.
[3]And R M B,Coleman R A.Enzymes of glycerolipid synthesis in eukaryotes[J].Annual Review of Biochemistry,1980,49(49):459-487.
[4]Coleman R,Bell R M.Triacylglycerol synthesis in isolated fat cells.Studies on the microsomal diacylglycerol acyltransferase activity using ethanol-dispersed diacylglycerols[J].Journal of Biological Chemistry,1976,251(15):4537-4543.
[5]Swanton E M,Saggerson E D.Glycerolipid metabolizing enzymes in rat ventricle and in cardiac myocytes[J].Biochimica et Biophysica Acta(BBA)-Lipids and Lipid Metabolism,1997,1346(1):93-102.
[6]Homa S T,Racowsky C,Mcgaughey R W.Lipid analysis of immature pig oocytes[J].J Reprod Fertil,1986,77(2):425-434.
[7]Winter A,Kr?mer W,Werner F A,et al.Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase(DGAT1)with variation at a quantitative trait locus for milk fat content[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99(14):9300-9305.
[8]Grisart B,Coppieters W,Farnir F,et al.Positional candidate cloning of a QTL in dairy cattle:identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition[J].Genome Research,2002,12(2):222.
[9]Grisart B,Farnir F,Karim L,et al.Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(8):2398-2403.
[10]Spelman R J,Ford C A,Mcelhinney P,et al.Characterization of the DGAT1 gene in the New Zealand dairy population[J].Journal of Dairy Science,2002,85(12):3514-3517.
[11]Thaller G,Kr?mer W,Winter A,et al.Effects of DGAT1 variants on milk production traits in German cattle breeds[J].Journal of Animal Science,2003,81(8):1911-1918.
[12]Weller J I,Golik M,Seroussi E,et al.Population-Wide Analysis of a QTL Affecting Milk-Fat Production in the Israeli Holstein Population[J].Journal of Dairy Science,2003,86(6):2219-2227.
[13]Kaupe B,Winter A,Fries R,et al.DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds[J].Journal of Dairy Research,2004,71(2):182-187.
[14]贾晋,马妍,孙东晓,等.中国荷斯坦牛DGAT1基因与产奶性状关联分析[J].畜牧兽医学报,2008,39(12):1661-1664.
[15]Oikonomou G,Angelopoulou K,Arsenos G,et al.The effects of polymorphisms in the DGAT1,leptin and growth hormone receptor gene loci on body energy,blood metabolic and reproductive traits of Holstein cows[J].Animal Genetics,2009,40(1):10-17.
[16]Pareek C S,Czarnik U,T,Pareek R S,et al.DGAT1 K232Aquantitative trait nucleotide polymorphism in Polish Black-andWhite cattle[J].Journal of Applied Genetics,2005,46(1):85-87.
[17]N?slund J,Fikse W F,Pielberg G R,et al.Frequency and Effect of the Bovine Acyl-CoA:Diacylglycerol Acyltransferase1(DGAT1)K232A Polymorphism in Swedish Dairy Cattle[J].Journal of Dairy Science,2008,91(5):2127-2134.
[18]卢振峰,刘莉莉,赵国丽,等.三河牛DGAT1基因K232A位点与产奶性状的关联分析[J].中国奶牛,2014,3:13-16.
[19]周利华,邓政,陈从英,等.DGAT1基因K232A突变位点在3个中国荷斯坦母牛群体中的基因型频率分布及其与产奶量的相关性[J].基因组学与应用生物学,2009,28(6):1087-1091.
[20]张晓东.奶牛DGAT1基因SNPs分析及其与产奶性能关系的研究[D].合肥:安徽农业大学,2009.
[21]徐秀容.DGAT1和DGAT2基因多态性与牛部分经济性状相关性的研究[D].杨凌:西北农林科技大学,2004.
[22]李峥.荷斯坦奶牛DGAT1等3个基因多态与泌乳性状的关联分析[D].大庆:黑龙江八一农垦大学,2016.
[23]殷骥,马海涛,赵国丽,等.中国荷斯坦牛DGAT1基因第8外显子对产奶性状的遗传效应分析[J].黑龙江畜牧兽医,2013,3:37-40.
[24]毛永江,陈仁金,陈莹,等.南方地区中国荷斯坦牛DGAT1基因多态性与泌乳性状的关联分析[J].中国农业科学,2010,43(14):2990-2995.
[25]马彦男,贺鹏迦,马永生,等.DGAT-1基因K232A突变位点对甘肃地区中国荷斯坦牛泌乳性状的影响[J].四川动物,2013,32(6):835-840.
[26]张驭纬,谭志东,付贤圣,等.贵州荷斯坦奶牛甘油二酯酰基转移酶基因多态性及其与产奶性状的关联分析[J].中国畜牧兽医,2013,40(12):56-61.
[27]娄佑武,吴志勇,王荣民,等.吉安西杂母牛DGAT1基因K232A遗传多态性及其对乳成分影响的分析[J].中国牛业科学,2015,41(3):18-21.
[28]邹荣婕.中国荷斯坦奶牛DGAT1、LOX-1基因多态性与产奶性状之间的关系研究[D].杨凌:西北农林科技大学,2007.
[29]巴塔拉伊.奶牛和水牛的MAP4K4和DGAT1基因对产奶性能和体细胞评分的影响与机理的研究[D].武汉:华中农业大学,2017.
[30]Maxa J,Neuditschko M,Russ I,et al.Genome-wide association mapping of milk production traits in Braunvieh cattle[J].Journal of Dairy Science,2012,95(9):5357-5364.
[31]Nayeri S,Sargolzaei M,Aboismail M K,et al.Genome-wide association for milk production and female fertility traits in Canadian dairy Holstein cattle[J].Bmc Genetics,2016,17(1):75.
[32]Bovenhuis H,Visker M H,Van Valenberg H J,et al.Effects of the DGAT1 polymorphism on test-day milk production traits throughout lactation[J].Journal of Dairy Science,2015,98(9):6572-6582.
[33]BartoňL,Bure?D,Kott T,et al.Associations of polymorphisms in bovine DGAT1,FABP4,FASN,and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls[J].Meat Science,2016,114:18-23.
[34]Bovenhuis H,Visker M H,Poulsen N A,et al.Effects of the diacylglycerol o-acyltransferase 1(DGAT1)K232A polymorphism on fatty acid,protein,and mineral composition of dairy cattle milk[J].Journal of Dairy Science,2016,99(4):3113-3123.
[35]Vanbergue E,Peyraud J L,Guinard-Flament J,et al.Effects of DGAT1 K232A polymorphism and milking frequency on milk composition and spontaneous lipolysis in dairy cows[J].Journal of Dairy Science,2016,99(7):5739-5749.
[36]Tomka J,Va?íkováK,OravcováM,et al.Effects of polymorphisms in DGAT1 and LEP genes on milk traits in Holstein primiparous cows[J].Mljekarstvo,2016,66(2):122-128.