大围山微型鸡和艾维茵肉鸡骨钙含量及CaBP基因表达差异研究
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摘要
钙结合蛋白(CaBP)可直接影响骨骼中钙的沉积,进而影响骨骼生长发育。为研究家鸡骨钙含量及其CaBP基因表达与骨强度的关系,选用大围山微型鸡和艾维茵肉鸡,分别测定4、8、12周龄时试验鸡的骨强度、骨钙沉积和小肠组织、软骨组织中CaBP基因的相对表达量。结果表明:4、8、12周龄时大围山微型鸡股骨骨强度、骨钙含量均显著高于艾维茵肉鸡(P<0.05),且大围山微型鸡小肠组织、软骨组织中CaBP基因的相对表达量显著高于艾维茵肉鸡(P<0.05)。相关性分析显示,骨钙含量与CaBP基因表达量和骨强度均呈正相关,且CaBP基因表达量和骨强度呈正相关。可见,具有较高的骨钙沉积是大围山微型鸡具有优良骨强度的重要原因,且CaBP基因可作为调控家鸡骨钙沉积的重要候选基因。
Calcium binding protein(CaBP) gene can directly affect calcium deposition in bone,which in turn affects bone growth and development.In order to study the relationship between bone calcium content,CaBP gene expression and bone strength in chickens,Daweishan mini chickens and Avian broilers were selected for detecting bone strength,bone calcium content of femur,CaBP gene expression in small intestine and cartilage tissues of chickens at of 4,8and 12 weeks of age.The results showed that the femur bone strength and bone calcium content of Daweishan mini chickens were significantly higher than those of Avian broilers at 4,8 and 12 weeks of age(P<0.05),and CaBP gene expression in small intestine and cartilage of Daweishan mini chickens were significantly higher than that of Avian broilers(P<0.05).Correlation analysis showed that bone calcium content was positively correlated with CaBP gene expression and bone strength,and CaBP gene expression was positively correlated with bone strength.It can be seen that the high bone calcium deposition contributes better bone strength for Daweishan mini chickens,and CaBP gene is an important candidate gene for the regulation of bone calcium deposition in chickens.
Calcium binding protein(CaBP) gene can directly affect calcium deposition in bone,which in turn affects bone growth and development.In order to study the relationship between bone calcium content,CaBP gene expression and bone strength in chickens,Daweishan mini chickens and Avian broilers were selected for detecting bone strength,bone calcium content of femur,CaBP gene expression in small intestine and cartilage tissues of chickens at of 4,8and 12 weeks of age.The results showed that the femur bone strength and bone calcium content of Daweishan mini chickens were significantly higher than those of Avian broilers at 4,8 and 12 weeks of age(P<0.05),and CaBP gene expression in small intestine and cartilage of Daweishan mini chickens were significantly higher than that of Avian broilers(P<0.05).Correlation analysis showed that bone calcium content was positively correlated with CaBP gene expression and bone strength,and CaBP gene expression was positively correlated with bone strength.It can be seen that the high bone calcium deposition contributes better bone strength for Daweishan mini chickens,and CaBP gene is an important candidate gene for the regulation of bone calcium deposition in chickens.
引文
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[2]豆腾飞,荣华,张丽春,等.TNF-α基因表达量及对云南大围山微型鸡及武定鸡股骨强度的影响[J].中国家禽,2015,37(5):10-13.
[3]RUBIN C J,BR?NDSTR?M H,WRIGHT D,et al.Quantitative trait loci for BMD and bone strength in an intercross between domestic and wildtype chickens[J].Journal of Bone and Mineral Research,2007,22(3):375-384.
[4]YANG C Y,OH T W,NAKAJIMA D,et al.Effects of habitual chitosan intake on bone mass,bone-related metabolic markers and duodenum CaBP D9K mRNA in ovariectomized SHRSP rats[J].J Nutr Sci Vitaminol(Tokyo),2002,48(5):371-378.
[5]WALDENSTED T L.Nutritional factors of importance for optimal health in broilers:A review[J].Animal Feed Science and Technology,2005(126):291-307.
[6]ADAMS D J,ACKERT-BICKNELL C L.Genetic regulation of bone strength:A review of animal model studies[J].Bonekey Reports,2015(4):714.doi:10.1038/bonekey.2015.83
[7]PODISI B K,KNOTT S A,DUNN I C,et al.Bone mineral density QTL at sexual maturity and end of lay[J].British Poultry Science,2012,53(6):763-769.
[8]TALATY P N,KATANBAF M N,HESTER P Y.Bone mineralization in male commercial broilers and its relationship to gait score[J].Poultry Science,2010,89(2):342-348.
[9]WILLIAMS B,WADDINGTON D,MURRAY D H,et al.Bone strength during growth:influence of growth rate on cortical porosity and mineralization[J].Calcified Tissue International,2004,74(3):236-245.
[10]GONZáLEZ-CERòN F,REKAYA R,AGGREY S E.Genetic analysis of bone quality traits and growth in a random mating broiler population[J].Poultry Science,2015,94(5):883-889.
[11]WANG Y,LI Y P,PAULSON C,et al.Wnt and the Wnt signaling pathway in bone development and disease[J].Front Biosci(Landmark Ed),2014(19):379-407.
[12]GUO J,SUN C,QU L,et al.Genetic architecture of bone quality variation in layer chickens revealed by a genomewide association study[J].Scientific Reports,2017(7):45317.
[13]KO S H,CHOI K C,OH G T,et al.Effect of dietary calcium and 1,25-(OH)2D3 on the expression of calcium transport genes in calbindin-D9k and-D28k double knockout mice[J].Biochemical and Biophysical Research Communications,2009,379(2):227-32.
[14]余晓丹,颜崇淮,沈晓明.缺锌对大鼠小肠粘膜维生素D受体及钙结合蛋白基因表达的影响[J].营养学报,2005,27(3):203-206.
[15]冯永淼,闫素梅,李慧英,等.日粮维生素A水平对肉鸡组织CaBP基因表达影响的研究[J].畜牧与兽医,2007,39(9):7-9.