腹腔镜器械应用生物膜清洗剂与多酶清洗剂清洗效果比较
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摘要
目的:探讨生物膜清洗剂与多酶清洗剂对腹腔镜器械清洗消毒效果比较。方法:选取609件临床使用后的腹腔镜手术器械,按照清洗方法的不同将其分为观察组(306件)和对照组(303件),观察组采用生物膜清洗剂清洗方法,对照组采用全效酶浸泡清洗方法。对两组清洗质量采取细菌检测与三磷酸腺苷(ATP)生物荧光检测的方法进行对比,评价两组腹腔镜器械清洗消毒效果。结果:1细菌检测合格率显示,观察组为97.4%,对照组为94.7%,两组相比差异有统计学意义(x2=5.868,P<0.05);2ATP生物荧光检测合格率显示,观察组为96.7%,对照组为93.7%,两组相比差异有统计学意义(x2=9.435,P<0.05)。结论:采用生物膜清洗剂进行内镜器械清洗消毒和灭菌,能有效地提高清洗消毒与灭菌的效果,适宜广泛在腹腔镜器械的清洗消毒中应用。
Objective: To discuss of the effects of biological membrane cleaning agent and enzyme's soaking to the results of the cleaning and disinfection of laparoscopic instruments. Methods: 609 laparoscopic surgical instruments were selected and randomly divided into the experimental group(n=306) with the method of biological membrane cleaning agent and the control group(n=303) with the method of full effect enzyme's soaking. The results of bacteria detection and ATP bioluminescence detection were compared to evaluate the efficacy of laparoscopic equipment cleaning of the two groups. Results: The qualification rate ofbacteria detection in the experimental group was 97.4% and the qualification rate in the control group was 94.7%. The difference was statistically significant(x2=5.868, P<0.05). The qualification rate of ATP bioluminescence detection in the experimental group was 96.7% and the qualification rate in the control group was 93.7%. The difference was statistically significant(x2=9.435, P<0.05). Conclusion: It can effectively improve the effect of cleaning, disinfection and sterilization with the biological membrane cleaning agent. It was suit for cleaning to laparoscopic instruments.
Objective: To discuss of the effects of biological membrane cleaning agent and enzyme's soaking to the results of the cleaning and disinfection of laparoscopic instruments. Methods: 609 laparoscopic surgical instruments were selected and randomly divided into the experimental group(n=306) with the method of biological membrane cleaning agent and the control group(n=303) with the method of full effect enzyme's soaking. The results of bacteria detection and ATP bioluminescence detection were compared to evaluate the efficacy of laparoscopic equipment cleaning of the two groups. Results: The qualification rate ofbacteria detection in the experimental group was 97.4% and the qualification rate in the control group was 94.7%. The difference was statistically significant(x2=5.868, P<0.05). The qualification rate of ATP bioluminescence detection in the experimental group was 96.7% and the qualification rate in the control group was 93.7%. The difference was statistically significant(x2=9.435, P<0.05). Conclusion: It can effectively improve the effect of cleaning, disinfection and sterilization with the biological membrane cleaning agent. It was suit for cleaning to laparoscopic instruments.
引文
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[15]赵琪琴,迟培环,王俊萍.腹腔镜器械的清洗、消毒及管理[J].腹腔镜外科杂志,2007,12(3):266-267.
[16]Fang Y,Shen Z,Li L,et al.A study of the efficacy of bacterial biofilm cleanout for gastrointestinal endoscopes[J].World J Gastroent erol,2010,16(8):1019-1024.
[17]Vickery K,Paikos A,Cossart Y.Removal of biofilm from endoscopes[J].Article in Portuguese,2012,10(46):91-98.
[2]杨华明,易滨.现代医学消毒学[M].北京:人民军医出版社,2002.
[3]董薪,于秀荣.不同浓度多酶清洗剂对手术器械清洗的效果[J].中华医院感染学杂志,2011,21(3):498-499.
[4]Hicure IM.Bacterial biofilms and infections.Silme busters[J].Nature,2000,408(6810):284-286.
[5]杨晓华.不同消毒灭菌方案对纤维支气管镜活检钳的消毒效果分析[J].中国医学装备,2014,11(10):64-67.
[6]李六亿.内镜医院感染现状、存在问题及管理对策[J].中华医院感染杂志,2005,15(4):423.
[7]陈彦丽,孙敬,柴海荣,等.生物膜特效清洗剂与全效酶对腰椎穿刺针清洗效果对比[J].中国消毒学杂志,2013,30(7):117-119.
[8]王素珍,刘正祥,扬帆,等.改进清洗方法提高医疗器械清洗洁净度[J].中华医院感染学杂志,2009,19(5):563-568.
[6]陈培琴,郭惜珍,黄旭华,等.手术器械清洗方法的对比研究[J].中华医院感染学杂志,2010,20(18):2806-2807.
[10]沙立民,蒋朝霞.灭菌治疗缺陷原因分析与对策[J].中华医院感染学杂志,2006,16(10):1134.
[11]Miner N,Harris V,Ebron T,et al.Sporicidal activity of disinfectantsas one possible cause for bacteria in patient-ready endoscopes[J].Gastroenterol Nurs,2007,30(4):285-290.
[12]Nelson DB.Recent advances in epidemiology and prevention of gastrointeslinal endoscopy related infections[J].Curr Opin Infect Dis,2005,18(3):326.
[13]魏静蓉,李斌,施建辉.手术器械清洁效果分析[J].中国医学装备,2007,4(2):46.
[14]冯彩霞.医院消毒供应中心手术器械的清洗消毒和管理[J].中国医学装备,2012,9(11)77-78.
[15]赵琪琴,迟培环,王俊萍.腹腔镜器械的清洗、消毒及管理[J].腹腔镜外科杂志,2007,12(3):266-267.
[16]Fang Y,Shen Z,Li L,et al.A study of the efficacy of bacterial biofilm cleanout for gastrointestinal endoscopes[J].World J Gastroent erol,2010,16(8):1019-1024.
[17]Vickery K,Paikos A,Cossart Y.Removal of biofilm from endoscopes[J].Article in Portuguese,2012,10(46):91-98.