酸性铬蓝K作探针测定药物中的卡马西平
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摘要
酸性铬蓝K在弱碱性溶液及溴代十六烷基吡啶存在下与卡马西平反应生成离子缔合物,产生的共振瑞利散射(RRS)信号显著增强,最大RRS峰位于376 nm波长处,线性范围为0.008～0.28 mg/L,检出限为0.0068 mg/L。据此建立了高灵敏、快速、准确测定卡马西平的新方法,探讨了体系的RRS光谱特征、适宜反应条件及共存物质的影响。方法用于药物中卡马西平的测定,回收率为98.18%～102.2%,相对标准偏差(RSD)(n=5)为2.2%～2.6%。
In the presence of a weak alkaline solution and cetylpyridinium bromide, acidic chrome blue K reacts with carbamazepine to form an ionic associate, resulting a distinctly enhanced of resonance Rayleigh scattering(RRS) signal. The maximum resonance Rayleigh scattering peak was located at 376 nm and the linear range was from 0.008 to 0.28 mg/L with a detection limit of 0.006 8 mg/L. A new rapid, sensitive and accurate RRS method for determination of carbamazepine was established. The RRS spectral characteristics of the ternary system, suitable reaction conditions and the effect of coexisting substances were discussed. The method was employed to determine the content of carbamazepine in drug. The recoveries and relative standard deviations(RSD),(n=5) were in the range from 98.18% to 102.2% and 2.2% to 2.6%, respectively.
In the presence of a weak alkaline solution and cetylpyridinium bromide, acidic chrome blue K reacts with carbamazepine to form an ionic associate, resulting a distinctly enhanced of resonance Rayleigh scattering(RRS) signal. The maximum resonance Rayleigh scattering peak was located at 376 nm and the linear range was from 0.008 to 0.28 mg/L with a detection limit of 0.006 8 mg/L. A new rapid, sensitive and accurate RRS method for determination of carbamazepine was established. The RRS spectral characteristics of the ternary system, suitable reaction conditions and the effect of coexisting substances were discussed. The method was employed to determine the content of carbamazepine in drug. The recoveries and relative standard deviations(RSD),(n=5) were in the range from 98.18% to 102.2% and 2.2% to 2.6%, respectively.
引文
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[2] Vosough M,Ghafghazi S,Sabetkasaei M.Chemometrics Enhanced HPLC-DAD Performance for Rapid Quantification of Carbamazepine and Phenobarbital in Human Serum Samples [J].Talanta,2014,119:17-23.
[3] Das S,Fleming D H,Mathew B S,et al.Determination of Serum Carbamazepine Concentration Using Dried Blood Spot Specimens for Resource Limited Settings [J].Hosp Pract,2017,45(2):46-50.
[4] Tuchil■ C,B?lea E D,Baconi D L.Development of a Simple HPLC Method for Determination of Carbamazepine in Human Plasma with Application in Therapeutic Drug Monitoring[J].Toxicol Lett,2015,238(2):S149-S149.
[5] Ma A L,Wang Y M,Duan H F,et al.Determination of Car Bamazepine Comcentration on DBS by HPLC [J].Chin J Hosp Pharm,2017,37(1):40-42 (in Chinese).(马爱玲,王漪檬,段虹飞,等.高效液相色谱法检测干血滤纸片中卡马西平的浓度[J].中国医院药学杂志,2017,37(1):40-42.)
[6] Qu L H,Fan Y J,Wang W J,et al.Development,Validation and Clinical Application of an Online-SPE-LC-HRMS/MS for Simultaneous Quantification of Phenobarbital,Phenytoin,Carbamazepine,and Its Active Metabolite Carbamazepine 10,11-epoxide [J].Talanta,2016,158:77-88.
[7] Mei S H,Yang L,Feng W X,et al.Application of LC-MS/MS Method for the Determination of Carbamazepine in Human Plasma and External Quality Assessment [J].China Pharm,2016,27(8):1 044-1 047 (in Chinese).(梅升辉,杨莉,冯卫星,等.LC-MS/MS 法测定人血浆中卡马西平的浓度及其在室间质评中的应用[J].中国药房,2016,27(8):1 044-1 047.)
[8] Lozano V A,Escandar G M.Second-order Advantage with Excitation-emission Photoinduced Fluorimetry for the Determination of the Antiepileptic Carbamazepine in Environmental Waters [J].Anal Chim Acta,2013,782:37-45.
[9] Basavaiah K,Abdulrahman S A M.Utilization of Bromination Reactions for the Determination of Carbamazepine Using Bromate-bromide Mixture as a Green Brominating Agent [J].Arab J Chem,2014,7(3):297-305.
[10] Teixeira J G,Veiga A,Candeias A J P,et al.Electro-oxidation of Carbamazepine Metabolites:Characterization and Influence in the Voltammetric Determination of the Parent Drug[J].Electrochim Acta,2013,108:51-65.
[11] Lavanya N,Sekar C,Ficarra S,et al.A Novel Disposable Electrochemical Sensor for Determination of Carbamazepine Based on Fe Doped SnO2 Nanoparticles Modified Screen-printed Carbon Electrode [J].Mater Sci Eng C,2016,62:53-60.