六味葛蓝降脂片HPLC指纹图谱建立及5种成分测定
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摘要
目的建立六味葛蓝降脂片(火麻仁、决明子、葛根等)HPLC指纹图谱,并测定葛根素、大豆苷、大豆苷元、橙黄决明素、大黄酚的含有量。方法该药物甲醇提取液的分析采用Phenomenex Gemini C_(18)色谱柱(250 mm×4.6 mm, 5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长280 nm。结果 10批样品中有12个共有峰,相似度大于0.997。5种成分在各自范围内线性关系良好(r>0.999 50),平均加样回收率96.0%～102.6%,RSD 0.54%～1.7%。结论该方法简便准确,可用于六味葛蓝降脂片的质量控制。
AIM To establish the HPLC fingerprints of Liuwei Gelan Jiangzhi Tablets(Cannabis Fructus, Cassiae Semen, Puerariae lobatae Radix, etc.) and to determine the contents of puerarin, daidzin, daidzein, aurantio-obtusin, chrysophanol.METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Phenomenex Gemini C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 280 nm.RESULTS There were twelve common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.997. Five constituents showed good linear relationships within their own ranges(r>0.999 50), whose average recoveries were 96.0%-102.6% with the RSDs of 0.54%-1.7%.CONCLUSION This simple and accurate method can be used for the quality control of Liuwei Gelan Jiangzhi Tablets.
AIM To establish the HPLC fingerprints of Liuwei Gelan Jiangzhi Tablets(Cannabis Fructus, Cassiae Semen, Puerariae lobatae Radix, etc.) and to determine the contents of puerarin, daidzin, daidzein, aurantio-obtusin, chrysophanol.METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Phenomenex Gemini C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 280 nm.RESULTS There were twelve common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.997. Five constituents showed good linear relationships within their own ranges(r>0.999 50), whose average recoveries were 96.0%-102.6% with the RSDs of 0.54%-1.7%.CONCLUSION This simple and accurate method can be used for the quality control of Liuwei Gelan Jiangzhi Tablets.
引文
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[21] 魏晓雨,姜国栋,陈泓,等.贞芪扶正颗粒的HPLC指纹图谱研究[J].中国药房,2017,28(33):4691-4694.
[22] 赵路,关琴笑,路丽,等.UHPLC法同时测定不同产地葛根中5种活性成分的含量[J].中药材,2015,38(3):473-475.
[23] 牛小花,陈洪源.清喉利咽颗粒的UPLC指纹图谱建立及主成分分析[J].中国药房,2017,28(6):826-830.
[24] 王颖,高琨.中药质量与质量控制方法综述[J].中国科技博览,2013(15):256.
[2] 卢熠,杨悦.降血脂药物的临床应用与展望[J].中国当代医药,2014,21(5):180-182.
[3] 郭东杰,袁肖寒,蔡曼,等.降血脂西药与中药研究现状与展望[J].黑龙江医药,2014,27(1):31-37.
[4] 杜志澎.高胆固醇与心血管危险因素相关性研究[J].中国当代医药,2013,20(2):7-8.
[5] 苏蓉,于德水.高脂血症的危害及防治[J].中国当代医药,2009,16(8):128-129.
[6] 刘石.调血脂药物的研究进展[J].北方药学,2013,10(10):94.
[7] 刁玉林,刘祎,孟大利.降血脂天然产物研究进展[J].沈阳药科大学学报,2009,26(12):1008-1012.
[8] 辛丹丹,宝贵荣,博·格日勒图,等.国内外降脂药物的研究进展[J].内蒙古中医药,2013,32(21):149-151.
[9] 农毅清,蒋林,罗宇东,等.六味葛蓝降脂片质量标准研究[J].食品与药品,2016,18(3):170-174.
[10] 罗宇东,蒋林,朱智德,等.六味葛蓝降脂片大鼠30d喂养试验研究[J].中国当代医药,2016,23(9):15-18.
[11] Du G,Zhao H Y,Song Y L,et al.Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column[J].J Sep Sci,2011,34(19):2576-2585.
[12] 金文姗,谈钰元,陈有根,等.高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量[J].中国中药杂志,2003,28(1):49-51.
[13] 张仁岗,刘福明.葛根的心血管药理作用研究进展[J].中药材,2001,24(7):535-537.
[14] 纪宝玉,裴莉昕,陈随清,等.野葛种质资源的随机扩增多态性DNA技术分析[J].中国实验方剂学杂志,2014,20(16):56-59.
[15] 郝延军,桑育黎,赵余庆,等.决明子的研究进展[J].中草药,2001,32(9):858-859.
[16] 刘斌,巩鸿霞,肖学凤,等.决明子化学成分及药理作用研究进展[J].药物评价研究,2010,33(4):312-315.
[17] 王小华,邓斌,张晓军,等.决明子黄酮类化合物的提取工艺研究[J].现代农业科技,2008(24):7-8,11.
[18] 张启伟,阴健,张俊,等.生、炒决明子及其煎剂中部分活性成分的比较[J].中草药,1996,27(2):79-81.
[19] 王晶彬,周旭,胡志方,等.基于指标成分和HPLC 指纹图谱检测的决明子化学品质评价研究[J].中草药,2008,39(6):917-919.
[20] 吴健,高家荣,韩燕全,等.芪归糖痛宁颗粒的 UPLC指纹图谱研究[J].中国药房,2014,25(11):1022-1024.
[21] 魏晓雨,姜国栋,陈泓,等.贞芪扶正颗粒的HPLC指纹图谱研究[J].中国药房,2017,28(33):4691-4694.
[22] 赵路,关琴笑,路丽,等.UHPLC法同时测定不同产地葛根中5种活性成分的含量[J].中药材,2015,38(3):473-475.
[23] 牛小花,陈洪源.清喉利咽颗粒的UPLC指纹图谱建立及主成分分析[J].中国药房,2017,28(6):826-830.
[24] 王颖,高琨.中药质量与质量控制方法综述[J].中国科技博览,2013(15):256.