追施氮肥对灌浆期沙地饲用燕麦叶片衰老特性的影响
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摘要
为探究氮素对饲用燕麦叶片衰老特性的影响,在科尔沁沙地,以燕麦为材料,于苗期、分蘖期、拔节期、抽穗期按照15%,40%,25%,20%比例,对燕麦追施0(CK),70,140,210,280 kg/hm~2纯氮,检测灌浆期燕麦叶片的叶面积、叶绿素含量、可溶性蛋白含量、抗氧化酶活性及膜脂过氧化程度。结果表明:追施氮肥对延缓叶片衰老、提高功能叶作用时间和效率有明显作用。在一定范围内随着施氮量的增加,饲用燕麦的叶面积、叶绿素含量均显著提高,可溶性蛋白含量增加,而MDA含量明显降低,SOD活性显著下降,POD活性和CAT活性显著增强,延缓叶片的衰老进程。当施氮量超过210 kg/hm~2后,继续增施氮肥旗叶叶面积、叶绿素含量及可溶性蛋白含量均明显下降,且不能持续提高花后叶片POD和CAT活性,但SOD活性显著增强,MDA含量显著升高。试验亦表明,清除植物体内氧自由基并非单一的酶作用结果,取决于SOD、POD、CAT保护酶系协同作用,在科尔沁沙地追施210 kg/hm~2氮肥,饲用燕麦叶片抗衰老能力最强。
To explore the effect of nitrogen on senescence characteristics of fodder oat leaves,in Horqin sandy land,using oat as material,pure nitrogen of 0( CK),70,140,210,280 kg/ha was applied to oats at seedling stage,tillering stage,jointing stage and heading stage in proportion of 15%,40%,25% and 20%. Leaf area,chlorophyll content,soluble protein content,antioxidant enzyme activity and membrane peroxidation degree were measured. The results showed that the application of nitrogen fertilizer had an obvious effect on delaying leaf senescence and improving functional leaf time and efficiency. In a certain range with the increased of nitrogen,chlorophyll content,leaf area of oat were significantly improved,the soluble protein content increased,while MDA content decreased,SOD activity decreased significantly,POD activity and CAT activity were significantly enhanced,delaying leaf senescence. When the nitrogen was more than 210 kg/ha,continuing to increase nitrogen application,flag leaf area and chlorophyll content and soluble protein content decreased significantly,and could not improve leaf POD and CAT activity after flowering,but the activity of SOD was significantly increased; MDA content increased significantly. This experiment also indicated that scavenging oxygen free radicals in plants was not a single enzyme action. It depended on the synergistic action of SOD,POD and CAT protective enzymes. The application of 210 kg/ha nitrogen fertilizer in the Horqin sandy land had the strongest anti-aging ability.
To explore the effect of nitrogen on senescence characteristics of fodder oat leaves,in Horqin sandy land,using oat as material,pure nitrogen of 0( CK),70,140,210,280 kg/ha was applied to oats at seedling stage,tillering stage,jointing stage and heading stage in proportion of 15%,40%,25% and 20%. Leaf area,chlorophyll content,soluble protein content,antioxidant enzyme activity and membrane peroxidation degree were measured. The results showed that the application of nitrogen fertilizer had an obvious effect on delaying leaf senescence and improving functional leaf time and efficiency. In a certain range with the increased of nitrogen,chlorophyll content,leaf area of oat were significantly improved,the soluble protein content increased,while MDA content decreased,SOD activity decreased significantly,POD activity and CAT activity were significantly enhanced,delaying leaf senescence. When the nitrogen was more than 210 kg/ha,continuing to increase nitrogen application,flag leaf area and chlorophyll content and soluble protein content decreased significantly,and could not improve leaf POD and CAT activity after flowering,but the activity of SOD was significantly increased; MDA content increased significantly. This experiment also indicated that scavenging oxygen free radicals in plants was not a single enzyme action. It depended on the synergistic action of SOD,POD and CAT protective enzymes. The application of 210 kg/ha nitrogen fertilizer in the Horqin sandy land had the strongest anti-aging ability.
引文
[1]朱爱民,张玉霞,王国君,王乐.科尔沁沙地4个燕麦品种的饲草产量特性比较[J].农学学报,2017,7(10):56-59. doi:CNKI:SUN:XKKJ. 0. 2017-10-011.Zhu A M,Zhang Y X,Wang G J,Wang L. 4 oat varie-ties in Horqin Sandy Land:comparison of forage yieldcharacteristics[J]. Journal of Agriculture, 2017,7(10):56-59. doi:CNKI:SUN:XKKJ. 0. 2017-10-011.
[2]田永雷,张玉霞,朱爱民,杜晓艳,王乐.施氮对科尔沁沙地饲用燕麦产量及氮肥利用率的影响[J].草原与草坪,2018,38(5):54-58. doi:10. 13817/j. cnki.cyycp. 2018. 05. 009.Tian Y L,Zhang Y X,Zhu A M,Du X Y,Wang L.Effects of nitrogen application on yield and nitrogen useefficiency of oat in Horqin sandy land[J]. Grassland andTurf,2018,38(5):54-58. doi:10. 13817/j. cnki.cyycp. 2018. 05. 009.
[3] Wu J B,Miao S,Zhang R Q,Miao H L,Wang S. Re-search progress on the mechanism of alfalfa cold resist-ance during overwintering period[J]. Agriculture,Forest-ry and Fisheries,2015,4(6):300-304.
[4]曲祥春,何中国,郝文媛,栾天浩,李玉发.我国燕麦生产现状及发展对策[J].杂粮作物,2006,26(3):233-235. doi:10. 3969/j. issn. 2095-0896. 2006. 03.035.Qu X C,He Z G,Hao W Y,Luan T H,Li Y F. Pro-ductive status and developing countermeasure of oat inChina[J]. Rain Fed Crops,2006,26(3):233-235.doi:10. 3969/j. issn. 2095-0896. 2006. 03. 035.
[5]吴浩,刘文辉,贾志锋,石红霄,梁国玲,马祥.青引1号燕麦生物量积累及其分配对磷肥的响应[J].中国草地学报,2018,40(6):18-25. doi:10. 16742/j.zgcdxb. 2018-06-03.Wu H,Liu W H,Jia Z F,Shi H X,Liang G L,Ma X.Response of biomass accumulation and distribution tophosphorus in oat(Avena sativa cv. Qingyin No. 1)[J].Chinese Journal of Grassland,2018,40(6):18-25.doi:10. 16742/j. zgcdxb. 2018-06-03.
[6]马力,周青平,颜红波,梁国玲,刘勇.氮肥与保水剂配施对青燕1号燕麦产量的影响[J].草业科学,2014,31(10):1929-1934. doi:10. 11829/j. issn.1001-0629. 2013-0695.Ma L,Zhou Q P,Yan H B,Liang G L,Liu Y. Effectsof nitrogen fertilizer and super absorbent polymers onyield of Avena sativa cv. Qingyan No. 1[J]. PrataculturalScience,2014,31(10):1929-1934. doi:10. 11829/j.issn. 1001-0629. 2013-0695.
[7]刘华.不同施肥量对6个燕麦品种产量的影响[J].青海草业,2018,27(3):24-27. doi:10. 3969/j.issn. 1008-1445. 2018. 03. 006.Liu H. Effects of different fertilization on yield of 6 oatscultivars[J]. Qinghai Prataculture,2018,27(3):24-27. doi:10. 3969/j. issn. 1008-1445. 2018. 03. 006.
[8]马祥,贾志锋,刘文辉,刘勇,魏小星,牛奎举.青海地区燕麦″3414″施肥效果及推荐施肥量[J].草业科学,2017,34(9):1906-1914. doi:10. 11829/j. issn.1001-0629. 2016-0586.Ma X,Jia Z F,Liu W H,Liu Y,Wei X X,Niu K J. Effcctof″3114″fertilization and recommended fertilizer quantityfor Avena sativa in Qinghai Arcea[J]. Pratacultural Sci-ence,2017,31(9):1906-1914. doi:10. 11829/j.issn. 1001-0629. 2016-0586.
[9]王乐,张玉霞,于华荣,郭园,朱爱民.苗期追施氮肥对沙地燕麦生长特性及叶绿体色素含量的影响[J].草原与草坪,2017,37(1):20-24. doi:10. 3969/j.issn. 1009-5500. 2017. 01. 005.Wang L,Zhang Y X,Yu H Y,Guo Y,Zhu A M.Effects of nitrogen fertilizer on growth and chlorophyllcontent of oat in seedling stage[J]. Grassland and Turf,2017,37(1):20-24. doi:10. 3969/j. issn. 1009-5500. 2017. 01. 005.
[10]李娜.不同施肥水平下糜子叶片衰老和活性氧代谢研究[D].杨凌:西北农林科技大学,2014:8-10.Li N. Under different fertilization broomcorn millet leafsenescence and activated oxygen metabolism studies[D]. Yangling:Northwest University of Agriculture andForestry Science and Technology,2014:8-10.
[11] Roca M, James C, Pruzinska A, Hortensteiner S,Thomas H,Ougham H. Analysis of the chlorophyll ca-tabolism pathway in leaves of an introgression senes-cence mutant of Lolium temulentum[J]. Phytochemis-tryj. phytochem,2004,65(9):1231-1238. doi. org/10. 1016/j. phytochem. 2004. 03. 022.
[12] Liu L,Li H X,Zeng H L,Cai Q S,Zhou X,Yin C X.Exogenous jasmonic acid and cytokinin antagonisticallyregulate rice flag leaf senescence by mediating chloro-phyll degradation,membrane deterioration and senes-cence-associated genes expression[J]. Plant GrowthRegulation,2016,35(2):366-376. doi. org/10. 1007/s00344-015-9539-0.
[13]严雯奕,叶胜海,董彦君,金庆生,张小明.植物叶片衰老相关研究进展[J].作物杂志,2010(4):4-9.Yan W Y,Ye S H,Dong Y J,Jin Q S,Zhang X M.Research progress related to plant leaf senescence[J].Crops,2010(4):4-9.
[14]曹蓓蓓.不同品种和栽培条件对小麦叶片衰老与碳氮平衡的影响[D].杨凌:西北农林科技大学,2017.Cao B B. Effects of different cultivars and cultivationson leaf senescence and C/N of wheat[D]. Yangling:Northwest University of Agriculture and Forestry Scienceand Technology,2017.
[15]王鹏杰,吴殿星,舒小丽.水稻衰老相关突变体的研究进展[J].核农学报,2018,32(3):497-505.doi:10. 11869/j. issn. 100-8551. 2018. 03. 0497.Wang P J,Wu D X,Shu X L. Advances of research onrice senescence mutants[J]. Journal of Nuclear Agricul-tural Sciences,2018,32(3):497-505. doi:10.11869/j. issn. 100-8551. 2018. 03. 0497.
[16]李健,赵宇,李锦锦,杨青华,谌涛,马兴立.不同基因型玉米叶片衰老与活性氧代谢的关系及其调控[J].华北农学报,2011,26(1):131-135. doi:10.7668/hbnxb. 2011. 01. 027.Li J,Zhao Y,Li J J,Yang Q H,Shen T,Ma X L. Astudy on the relationship between the leaf senescenceand activate oxygen metabolism of different maize varie-ties and their regulation[J]. Acta Agriculturae Boreali-Sinica,2011,26(1):131-135. doi:10. 7668/hbnxb.2011. 01. 027.
[17]陈义珍,董合忠.棉花衰老和熟相形成的生理生态与调控研究进展[J].应用生态学报,2016,27(2):643-651. doi:10. 13287/j. 1001-9332. 201602. 008.Chen Y Z,Dong H Z. Eco-physiology and regulation ofleaf senescence and maturity performance in cotton:Areview[J]. Chinese Journal of Applied Ecology,2016,27(2):643-651. doi:10. 13287/j. 1001-9332.201602. 008.
[18]王乐,张玉霞,于华荣,郭园,朱爱民.氮肥对沙地燕麦生长特性及产量的影响[J].草业科学,2017,34(7):1516-1521. doi:10. 11829/j. issn. 1001-0629. 2016-0490.Wang L,Zhang Y X,Yu H R,Guo Y,Zhu A M.Effect of nitrogen fertilizer application on growth charac-teristics and yield of oats in sandy soil[J]. Pratacultur-al Science,2017,34(7):1516-1521. doi:10. 11829/j. issn. 1001-0629. 2016-0490.
[19]丁锦峰,成亚梅,黄正金,李春燕,郭文善,朱新开.稻茬小麦不同氮效率群体花后物质生产与衰老特性差异分析[J].中国农业科学,2015,48(6):1063-1073. doi:10. 3864/j. issn. 0578-1752. 2015. 06. 03.Ding J F,Cheng Y M,Huang Z J,Li C Y,Guo W S,Zhu X K. Difference analysis of post-anthesis matterproduction and senescence characteristics among differ-ent nitrogen efficiency populations in wheat followingrice[J]. Scientia Agricultura Sinica,2015,48(6):1063-1073. doi:10. 3864/j. issn. 0578-1752. 2015.06. 03.
[20]蔡红梅.超量表达谷氨酰胺合成酶、硝酸根转运蛋白和铵离子转运蛋白家族1基因对水稻氮代谢的功能研究[D].武汉:华中农业大学,2009.Cai H M. Functional analysis of overexpressing gluta-mine synthetase,nitrate transporter and ammonium trans-porter 1 genes for nitrogen assimilation in rice[D]. Wu-han:Huazhong Agricultural University,2009.
[21] Matile P. Biochemistry of Indian summer:physiology ofautumnal leaf coloration[J]. Experimental Gerontology,2000,35(2):145-158. doi:10. 1016/S0531-5565(00)00081-4.
[22]郝再彬,苍品,徐仲.生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004.Hao Z B,Cang P,Xu Z. Physiological experiments[M]. Harbin:Harbin Institute of Technology Press,2004
[23]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.Zou Q. Guidance for plant physiology experiments[M]. Beijing:China Agriculture Press,2000
[24]李淑文,文宏达,周彦珍,李雁鸣,肖凯.不同氮效率小麦品种氮素吸收和物质生产特性[J].中国农业科学,2006,39(10):1992-2000. doi:10. 3321/j.issn:0578-1752. 2006. 10. 007.Li S W,Wen H D,Zhou Y Z,Li Y M,Xiao K. Char-acterization of nitrogen uptake and dry matter productionin wheat varieties with different N efficiency[J]. Scien-tia Agricultura Sinica,2006,39(10):1992-2000.doi:10. 3321/j. issn:0578-1752. 2006. 10. 007.
[25]路文静,张树华,郭程瑾,段巍巍,肖凯.不同氮素利用效率小麦品种的氮效率相关生理参数研究[J].植物营养与肥料学报,2009,15(5):985-991. doi:10. 3321/j. issn:1008-505X. 2009. 05. 001.Lu W J,Zhang S H,Guo C J,Duan W W,Xiao K.Studies on the physiological parameters related to nitro-gen use efficiency in wheat cultivars with different nitro-gen utilization[J]. Plant Nutrition and Fertilizer Sci-ence,2009,15(5):985-991. doi:10. 3321/j. issn:1008-505X. 2009. 05. 001.
[26]袁昌洪,韩冬,杨菲,杨再强.氮肥对茶树春季光合、抗衰老特性及内源激素含量的影响[J].南京林业大学学报(自然科学版),2016,40(5):67-73.doi:10. 3969/j. issn. 1000-2006. 2016. 05. 011.Yuan C H,Han D,Yang F,Yang Z Q. Effects of nitro-gen fertilization level in soil on physiological characteris-tics and quality of tea leaves[J]. Journal of NanjingForestry University(Natural Science Edition),2016,40(5):67-73. doi:10. 3969/j. issn. 1000-2006. 2016.05. 011.
[27] Makino A,Mac T,Ohire K. Changes in photosyntheticcapacity in rice leaves from emergence through senes-cence. analysis from ribulose-1,5-bisphosphate carbox-ylase and leaf conductance[J]. Plant and Cell Physiol,1984,25:511-514. doi. org/10. 1093/oxfordjournals.pcp. a076740.
[28]张承烈,杨成德,梁厚果.萝卜离体子叶衰老与膜脂过氧化的关系[J].植物生理学报,1990,16(3):227-232.Zhang C L,Yang C D,Liang H G. The relationship be-tween senescence and membrane lipid peroxidation indetached cotyledons of radish[J]. Acta Phytophysiolog-ica Sinica,1990,16(3):227-232.
[29]朱爱民,张玉霞,王显国,田永雷.秋末刈割对沙地苜蓿冬季根颈抗氧化酶活性及脯氨酸含量的影响[J].草地学报,2018,26(1):222-230. doi:10.11733/j. issn. 1007-0435. 2018. 01. 028.Zhu A M,Zhang Y X,Wang X G,Tian Y L. Effects ofcutting treatments in late autumn on antioxidant enzymeactivity and proline content in crown of sandy land alfalfain winter[J]. Acta Agrestia sinica,2018,26(1):222-230. doi:10. 11733/j. issn. 1007-0435. 2018. 01. 028.
[30]施大伟,陈国祥,张成军,王萍,王静,庄丽娟,陈利.两种高产小麦旗叶自然衰老过程中生理特性的比较[J].西北农业学报,2005,14(2):23-26.doi:10. 3969/j. issn. 1004-1389. 2005. 02. 006.Shi D W,Chen G X,Zhang C J,Wang P,Wang J,Zhuang L J,Chen L. Comparison of physiological prop-erties between two high-yield wheat varieties during flagnatural senescence[J]. Acta Agriculturae Boreali-Occi-dentalis Sinica,2005,14(2):23-26. doi:10. 3969/j.issn. 1004-1389. 2005. 02. 006.
[31]陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学通报,1989,6(4):211-217.Chen S Y. Membrane lipid peroxidation and plant stress[J]. Botanical Bulletin,1989,6(4):211-217.
[32]李京涛,姜雯,张玉梅,刘树堂,林琪.长期定位施肥对冬小麦后期根系衰老和产量的影响[J].华北农学报,2007,22(5):138-141. doi:10. 7668/hbnxb.2007. 05. 034.Li J T,Jiang W,Zhang Y M,Liu S T,Lin Q. Effectsof long-term locating fertilization on decrepitude index ofroot system and yield of winter wheat at later growthstage[J]. Acta Agriculturae Boreali-Sinica,2007,22(5):138-141. doi:10. 7668/hbnxb. 2007. 05. 034.
[2]田永雷,张玉霞,朱爱民,杜晓艳,王乐.施氮对科尔沁沙地饲用燕麦产量及氮肥利用率的影响[J].草原与草坪,2018,38(5):54-58. doi:10. 13817/j. cnki.cyycp. 2018. 05. 009.Tian Y L,Zhang Y X,Zhu A M,Du X Y,Wang L.Effects of nitrogen application on yield and nitrogen useefficiency of oat in Horqin sandy land[J]. Grassland andTurf,2018,38(5):54-58. doi:10. 13817/j. cnki.cyycp. 2018. 05. 009.
[3] Wu J B,Miao S,Zhang R Q,Miao H L,Wang S. Re-search progress on the mechanism of alfalfa cold resist-ance during overwintering period[J]. Agriculture,Forest-ry and Fisheries,2015,4(6):300-304.
[4]曲祥春,何中国,郝文媛,栾天浩,李玉发.我国燕麦生产现状及发展对策[J].杂粮作物,2006,26(3):233-235. doi:10. 3969/j. issn. 2095-0896. 2006. 03.035.Qu X C,He Z G,Hao W Y,Luan T H,Li Y F. Pro-ductive status and developing countermeasure of oat inChina[J]. Rain Fed Crops,2006,26(3):233-235.doi:10. 3969/j. issn. 2095-0896. 2006. 03. 035.
[5]吴浩,刘文辉,贾志锋,石红霄,梁国玲,马祥.青引1号燕麦生物量积累及其分配对磷肥的响应[J].中国草地学报,2018,40(6):18-25. doi:10. 16742/j.zgcdxb. 2018-06-03.Wu H,Liu W H,Jia Z F,Shi H X,Liang G L,Ma X.Response of biomass accumulation and distribution tophosphorus in oat(Avena sativa cv. Qingyin No. 1)[J].Chinese Journal of Grassland,2018,40(6):18-25.doi:10. 16742/j. zgcdxb. 2018-06-03.
[6]马力,周青平,颜红波,梁国玲,刘勇.氮肥与保水剂配施对青燕1号燕麦产量的影响[J].草业科学,2014,31(10):1929-1934. doi:10. 11829/j. issn.1001-0629. 2013-0695.Ma L,Zhou Q P,Yan H B,Liang G L,Liu Y. Effectsof nitrogen fertilizer and super absorbent polymers onyield of Avena sativa cv. Qingyan No. 1[J]. PrataculturalScience,2014,31(10):1929-1934. doi:10. 11829/j.issn. 1001-0629. 2013-0695.
[7]刘华.不同施肥量对6个燕麦品种产量的影响[J].青海草业,2018,27(3):24-27. doi:10. 3969/j.issn. 1008-1445. 2018. 03. 006.Liu H. Effects of different fertilization on yield of 6 oatscultivars[J]. Qinghai Prataculture,2018,27(3):24-27. doi:10. 3969/j. issn. 1008-1445. 2018. 03. 006.
[8]马祥,贾志锋,刘文辉,刘勇,魏小星,牛奎举.青海地区燕麦″3414″施肥效果及推荐施肥量[J].草业科学,2017,34(9):1906-1914. doi:10. 11829/j. issn.1001-0629. 2016-0586.Ma X,Jia Z F,Liu W H,Liu Y,Wei X X,Niu K J. Effcctof″3114″fertilization and recommended fertilizer quantityfor Avena sativa in Qinghai Arcea[J]. Pratacultural Sci-ence,2017,31(9):1906-1914. doi:10. 11829/j.issn. 1001-0629. 2016-0586.
[9]王乐,张玉霞,于华荣,郭园,朱爱民.苗期追施氮肥对沙地燕麦生长特性及叶绿体色素含量的影响[J].草原与草坪,2017,37(1):20-24. doi:10. 3969/j.issn. 1009-5500. 2017. 01. 005.Wang L,Zhang Y X,Yu H Y,Guo Y,Zhu A M.Effects of nitrogen fertilizer on growth and chlorophyllcontent of oat in seedling stage[J]. Grassland and Turf,2017,37(1):20-24. doi:10. 3969/j. issn. 1009-5500. 2017. 01. 005.
[10]李娜.不同施肥水平下糜子叶片衰老和活性氧代谢研究[D].杨凌:西北农林科技大学,2014:8-10.Li N. Under different fertilization broomcorn millet leafsenescence and activated oxygen metabolism studies[D]. Yangling:Northwest University of Agriculture andForestry Science and Technology,2014:8-10.
[11] Roca M, James C, Pruzinska A, Hortensteiner S,Thomas H,Ougham H. Analysis of the chlorophyll ca-tabolism pathway in leaves of an introgression senes-cence mutant of Lolium temulentum[J]. Phytochemis-tryj. phytochem,2004,65(9):1231-1238. doi. org/10. 1016/j. phytochem. 2004. 03. 022.
[12] Liu L,Li H X,Zeng H L,Cai Q S,Zhou X,Yin C X.Exogenous jasmonic acid and cytokinin antagonisticallyregulate rice flag leaf senescence by mediating chloro-phyll degradation,membrane deterioration and senes-cence-associated genes expression[J]. Plant GrowthRegulation,2016,35(2):366-376. doi. org/10. 1007/s00344-015-9539-0.
[13]严雯奕,叶胜海,董彦君,金庆生,张小明.植物叶片衰老相关研究进展[J].作物杂志,2010(4):4-9.Yan W Y,Ye S H,Dong Y J,Jin Q S,Zhang X M.Research progress related to plant leaf senescence[J].Crops,2010(4):4-9.
[14]曹蓓蓓.不同品种和栽培条件对小麦叶片衰老与碳氮平衡的影响[D].杨凌:西北农林科技大学,2017.Cao B B. Effects of different cultivars and cultivationson leaf senescence and C/N of wheat[D]. Yangling:Northwest University of Agriculture and Forestry Scienceand Technology,2017.
[15]王鹏杰,吴殿星,舒小丽.水稻衰老相关突变体的研究进展[J].核农学报,2018,32(3):497-505.doi:10. 11869/j. issn. 100-8551. 2018. 03. 0497.Wang P J,Wu D X,Shu X L. Advances of research onrice senescence mutants[J]. Journal of Nuclear Agricul-tural Sciences,2018,32(3):497-505. doi:10.11869/j. issn. 100-8551. 2018. 03. 0497.
[16]李健,赵宇,李锦锦,杨青华,谌涛,马兴立.不同基因型玉米叶片衰老与活性氧代谢的关系及其调控[J].华北农学报,2011,26(1):131-135. doi:10.7668/hbnxb. 2011. 01. 027.Li J,Zhao Y,Li J J,Yang Q H,Shen T,Ma X L. Astudy on the relationship between the leaf senescenceand activate oxygen metabolism of different maize varie-ties and their regulation[J]. Acta Agriculturae Boreali-Sinica,2011,26(1):131-135. doi:10. 7668/hbnxb.2011. 01. 027.
[17]陈义珍,董合忠.棉花衰老和熟相形成的生理生态与调控研究进展[J].应用生态学报,2016,27(2):643-651. doi:10. 13287/j. 1001-9332. 201602. 008.Chen Y Z,Dong H Z. Eco-physiology and regulation ofleaf senescence and maturity performance in cotton:Areview[J]. Chinese Journal of Applied Ecology,2016,27(2):643-651. doi:10. 13287/j. 1001-9332.201602. 008.
[18]王乐,张玉霞,于华荣,郭园,朱爱民.氮肥对沙地燕麦生长特性及产量的影响[J].草业科学,2017,34(7):1516-1521. doi:10. 11829/j. issn. 1001-0629. 2016-0490.Wang L,Zhang Y X,Yu H R,Guo Y,Zhu A M.Effect of nitrogen fertilizer application on growth charac-teristics and yield of oats in sandy soil[J]. Pratacultur-al Science,2017,34(7):1516-1521. doi:10. 11829/j. issn. 1001-0629. 2016-0490.
[19]丁锦峰,成亚梅,黄正金,李春燕,郭文善,朱新开.稻茬小麦不同氮效率群体花后物质生产与衰老特性差异分析[J].中国农业科学,2015,48(6):1063-1073. doi:10. 3864/j. issn. 0578-1752. 2015. 06. 03.Ding J F,Cheng Y M,Huang Z J,Li C Y,Guo W S,Zhu X K. Difference analysis of post-anthesis matterproduction and senescence characteristics among differ-ent nitrogen efficiency populations in wheat followingrice[J]. Scientia Agricultura Sinica,2015,48(6):1063-1073. doi:10. 3864/j. issn. 0578-1752. 2015.06. 03.
[20]蔡红梅.超量表达谷氨酰胺合成酶、硝酸根转运蛋白和铵离子转运蛋白家族1基因对水稻氮代谢的功能研究[D].武汉:华中农业大学,2009.Cai H M. Functional analysis of overexpressing gluta-mine synthetase,nitrate transporter and ammonium trans-porter 1 genes for nitrogen assimilation in rice[D]. Wu-han:Huazhong Agricultural University,2009.
[21] Matile P. Biochemistry of Indian summer:physiology ofautumnal leaf coloration[J]. Experimental Gerontology,2000,35(2):145-158. doi:10. 1016/S0531-5565(00)00081-4.
[22]郝再彬,苍品,徐仲.生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004.Hao Z B,Cang P,Xu Z. Physiological experiments[M]. Harbin:Harbin Institute of Technology Press,2004
[23]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.Zou Q. Guidance for plant physiology experiments[M]. Beijing:China Agriculture Press,2000
[24]李淑文,文宏达,周彦珍,李雁鸣,肖凯.不同氮效率小麦品种氮素吸收和物质生产特性[J].中国农业科学,2006,39(10):1992-2000. doi:10. 3321/j.issn:0578-1752. 2006. 10. 007.Li S W,Wen H D,Zhou Y Z,Li Y M,Xiao K. Char-acterization of nitrogen uptake and dry matter productionin wheat varieties with different N efficiency[J]. Scien-tia Agricultura Sinica,2006,39(10):1992-2000.doi:10. 3321/j. issn:0578-1752. 2006. 10. 007.
[25]路文静,张树华,郭程瑾,段巍巍,肖凯.不同氮素利用效率小麦品种的氮效率相关生理参数研究[J].植物营养与肥料学报,2009,15(5):985-991. doi:10. 3321/j. issn:1008-505X. 2009. 05. 001.Lu W J,Zhang S H,Guo C J,Duan W W,Xiao K.Studies on the physiological parameters related to nitro-gen use efficiency in wheat cultivars with different nitro-gen utilization[J]. Plant Nutrition and Fertilizer Sci-ence,2009,15(5):985-991. doi:10. 3321/j. issn:1008-505X. 2009. 05. 001.
[26]袁昌洪,韩冬,杨菲,杨再强.氮肥对茶树春季光合、抗衰老特性及内源激素含量的影响[J].南京林业大学学报(自然科学版),2016,40(5):67-73.doi:10. 3969/j. issn. 1000-2006. 2016. 05. 011.Yuan C H,Han D,Yang F,Yang Z Q. Effects of nitro-gen fertilization level in soil on physiological characteris-tics and quality of tea leaves[J]. Journal of NanjingForestry University(Natural Science Edition),2016,40(5):67-73. doi:10. 3969/j. issn. 1000-2006. 2016.05. 011.
[27] Makino A,Mac T,Ohire K. Changes in photosyntheticcapacity in rice leaves from emergence through senes-cence. analysis from ribulose-1,5-bisphosphate carbox-ylase and leaf conductance[J]. Plant and Cell Physiol,1984,25:511-514. doi. org/10. 1093/oxfordjournals.pcp. a076740.
[28]张承烈,杨成德,梁厚果.萝卜离体子叶衰老与膜脂过氧化的关系[J].植物生理学报,1990,16(3):227-232.Zhang C L,Yang C D,Liang H G. The relationship be-tween senescence and membrane lipid peroxidation indetached cotyledons of radish[J]. Acta Phytophysiolog-ica Sinica,1990,16(3):227-232.
[29]朱爱民,张玉霞,王显国,田永雷.秋末刈割对沙地苜蓿冬季根颈抗氧化酶活性及脯氨酸含量的影响[J].草地学报,2018,26(1):222-230. doi:10.11733/j. issn. 1007-0435. 2018. 01. 028.Zhu A M,Zhang Y X,Wang X G,Tian Y L. Effects ofcutting treatments in late autumn on antioxidant enzymeactivity and proline content in crown of sandy land alfalfain winter[J]. Acta Agrestia sinica,2018,26(1):222-230. doi:10. 11733/j. issn. 1007-0435. 2018. 01. 028.
[30]施大伟,陈国祥,张成军,王萍,王静,庄丽娟,陈利.两种高产小麦旗叶自然衰老过程中生理特性的比较[J].西北农业学报,2005,14(2):23-26.doi:10. 3969/j. issn. 1004-1389. 2005. 02. 006.Shi D W,Chen G X,Zhang C J,Wang P,Wang J,Zhuang L J,Chen L. Comparison of physiological prop-erties between two high-yield wheat varieties during flagnatural senescence[J]. Acta Agriculturae Boreali-Occi-dentalis Sinica,2005,14(2):23-26. doi:10. 3969/j.issn. 1004-1389. 2005. 02. 006.
[31]陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学通报,1989,6(4):211-217.Chen S Y. Membrane lipid peroxidation and plant stress[J]. Botanical Bulletin,1989,6(4):211-217.
[32]李京涛,姜雯,张玉梅,刘树堂,林琪.长期定位施肥对冬小麦后期根系衰老和产量的影响[J].华北农学报,2007,22(5):138-141. doi:10. 7668/hbnxb.2007. 05. 034.Li J T,Jiang W,Zhang Y M,Liu S T,Lin Q. Effectsof long-term locating fertilization on decrepitude index ofroot system and yield of winter wheat at later growthstage[J]. Acta Agriculturae Boreali-Sinica,2007,22(5):138-141. doi:10. 7668/hbnxb. 2007. 05. 034.