摘要
以杜仲的幼嫩茎段为试材,采用组织培养的方法,研究了消毒时间、基本培养基、NAA浓度、6-BA浓度、低温处理时间及培养环境对杜仲腋芽诱导及其分化的影响,以期为建立杜仲高效组织培养体系提供依据。结果表明:最佳消毒时间为9 min,污染率与死亡率均较低,分别为10.00%和26.67%;腋芽诱导的基本培养基以MS培养基为最佳,诱导率达91.66%;诱导培养基为MS+0.2 mg·L~(-1) 6-BA+0.03 mg·L~(-1) NAA时,腋芽的诱导率较高,达93.33%,且生长良好;当低温处理时间为24 h时,外植体诱导效果最好,且褐化率最低,为5.00%;与黑暗培养相比,光照培养条件更加有利于杜仲的腋芽诱导和生长;MS+1.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA为愈伤分化的最佳培养基,MS+2.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA为芽增殖的最佳培养基。
The young stem segments of Eucommia ulmoides Oliver were used as materials,tissue culture method was used,the effects of disinfection time,basic medium,NAA concentration,6-BA concentration,low temperature treatment time and culture environment on the induction of Eucommia ulmoides Oliver axillary buds and the differentiation of Eucommia ulmoides Oliver were investigated,in order to provide a basis for establishing an efficient tissue culture system of Eucommia ulmoides Oliver.The results showed that the best disinfection time was 9 minutes,and the rate of pollution and mortality were lower,10.00% and 26.67% respectively;the basic medium for axillary buds induction was MS medium,with an induction rate of 91.66%;when the induction medium was MS+0.2 mg·L~(-1) 6-BA+0.03 mg·L~(-1) NAA,the induction rate of axillary buds was 93.33% and it grew well;when the low temperature treatment time was 24 hours,explant induction effect was the best,and browning rate was the lowest,5.00%;compared with dark culture,light culture conditions were more conducive to the induction and growth of axillary buds of Eucommia ulmoides;MS+1.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA was the best medium for callus differentiation,MS+2.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA was the best medium for bud proliferation.
引文
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