摘要
目的研究宽叶打碗花Calystegia sepium中的化学成分。方法利用硅胶柱色谱、凝胶柱色谱、反相柱色谱和制备液相色谱等方法进行分离纯化,通过理化性质、波谱数据并结合文献报道数据鉴定化合物结构。结果从宽叶打碗花中分离得到20个化合物,分别鉴定为酪醇(1)、槲皮素-3-O-β-D-吡喃葡糖苷(2)、野黄芩素-7-O-β-吡喃葡萄糖苷(3)、紫云英苷(4)、山柰酚-3-O-半乳糖苷(5)、山柰酚-3-O-β-刺槐双糖苷(6)、烟花苷(7)、芦丁(8)、尿苷(9)、它乔糖苷(10)、2-甲氧基-4-(2′-羟乙基)-苯基-1-O-β-D-吡喃葡萄糖苷(11)、4-羟基苯乙醇-4-O-β-D-吡喃葡萄糖苷(12)、polybotrin(13)、吡啶-3,5-二甲酰胺(14)、茵芋苷(15)、苯甲醇-β-D-葡萄糖苷(16)、5-羟基-2-羟甲基吡啶(17)、云杉苷(18)、5-羟甲基糠醛(19)、sessiline(20)。结论所有化合物均为首次从该植物中分离得到。
Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol(1), quercetin-3-O-β-D-glucopyranoside(2), scutellarein-7-O-β-glucopyranoside(3), kaempferol-3-O-β-D-glucopyranoside(4), kaempferol-3-O-galactoside(5), kaempferol-3-O-β-robinobioside(6), nicotiflorin(7), rutin(8), uridine(9), tachioside(10), 2-methoxy-4-(2′-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside(11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside(12), polybotrin(13), pyridine-3,5-dicarboamide(14), skimmin(15), benzylalcohol-β-D-glucopyranoside(16), 5-hydroxyl-2-hydroxymethylpyridine(17), picein(18), 5-hydroxymethyl-furaldehyde(19), and sessiline(20). Conclusion Compounds 1—20 are isolated from Calystegia sepium for the first time.
引文
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