节节红化学成分研究
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摘要
目的:探究艾纳香属植物节节红的化学成分。方法:采用硅胶、凝胶Sephadex LH-20及ODS反相等柱色谱方法对节节红茎叶的乙酸乙酯萃提物进行分离纯化,通过谱学分析(质谱、核磁共振等)技术对所得化合物进行结构鉴定。结果:从节节红80%乙醇-水提取物的乙酸乙酯部位分离得到9个化合物,分别鉴定为:芹菜素-7-O-β-D-吡喃葡萄糖苷(1)、芹菜素(2)、咖啡酸乙酯(3)、木犀草素(4)、α-羟基苯丙酸(5)、小麦黄素(6)、金圣草黄素(7)、羽扇豆醇(8)和32,33,34-三甲基-细菌藿烷-16-烯-3-O-β-D-吡喃葡萄糖苷(9)。结论:首次对节节红的化学成分进行分离鉴定并通过文献调研这些化合物的活性作用机制,以期探寻节节红的活性化学物质基础。
Objective:To explore the chemical constituents of Blumea fistulosa(Roxb.)Kurz.Methods:The compounds were isolated and purified by column chromatography by repeated silica gel,sephadex LH-20 gel or ODS etc.from EtOAc extraction of the B.fistulosa.The structures of the isolated compounds were elucidated on the basis of spectroscopic analysis(MS,NMR).Results:9 compounds were isolated,which were identified as:apigenin-7-O-β-D-glucopyranoside(1),apigenin(2),ethyl caffeate(3),luteolin(4),3-phenyllactic acid(5),tricin(6),chrysoeriol(7),lupeol(8)and 32,33,34-trimethyl-bacteriohopan-16-ene3-O-β-D-glucopyranoside(9).Conclusion:The chemical constituents of B.fistulosa were isolated and identified for the first time,and the active mechanism of these compounds was postulated through literature investigation.The study lays a foundation for further study of active substances of B.fistulosa.
Objective:To explore the chemical constituents of Blumea fistulosa(Roxb.)Kurz.Methods:The compounds were isolated and purified by column chromatography by repeated silica gel,sephadex LH-20 gel or ODS etc.from EtOAc extraction of the B.fistulosa.The structures of the isolated compounds were elucidated on the basis of spectroscopic analysis(MS,NMR).Results:9 compounds were isolated,which were identified as:apigenin-7-O-β-D-glucopyranoside(1),apigenin(2),ethyl caffeate(3),luteolin(4),3-phenyllactic acid(5),tricin(6),chrysoeriol(7),lupeol(8)and 32,33,34-trimethyl-bacteriohopan-16-ene3-O-β-D-glucopyranoside(9).Conclusion:The chemical constituents of B.fistulosa were isolated and identified for the first time,and the active mechanism of these compounds was postulated through literature investigation.The study lays a foundation for further study of active substances of B.fistulosa.
引文
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[21] 彭钰芳,邹文韬,许传莲.杭白菊中黄酮类化合物及其抑制人源肿瘤细胞增殖活性的研究[J].中国药学杂志,2010,45(19):1454-1459.
[22] 龚春燕,张道敬,魏鸿刚,等.多粘类芽孢杆菌HY96-2发酵液化学成分研究[J].天然产物研究与开发,2009,21(3):379-381,387.
[23] ITHO A,SADANARI H,TAKEMOTO M,et al.Tricin inhibits CCL5 induction required for the efficient growth of human cytomegalovirus[J].Microbiol Immunol,2018,62(5):341-347.
[24] YANG Y,ZHOU X,XIAO M,et al.Discovery of chrysoeriol,a PI3K-AKT-mTOR pathway inhibitor with potent antitumor activity against human multiple myeloma cells in vitro[J].J Huazhong Univ Sci Technolog Med Sci,2010,30(6):734-740.
[25] WANG M,CUI H X,SUN C,et al.Effect of lupeol on migration and invasion of human breast cancer MDA-MB-231 cells and its mechanism[J].Acta Pharm Sin,2016,51(4):558.
[2] 陈铭,金慧子,严岚,等.艾纳香黄酮类化学成分的研究[J].天然产物研究与开发,2010,22(6):991-994.
[3] 韦金桃,陈艺波,许婉婷,等.芹菜素抗小鼠心肌缺氧作用的实验研究[J].西北民族大学学报(自然科学版),2017,38(1):21-23.
[4] 广西壮族自治区药物研究所.广西药用植物名录[M].北京:科学出版社,2010:621
[5] 蓝鸣生,罗超,谭昌恒.等.山风乙酸乙酯部位的化学成分研究[J].中药材,2012,35(2):229-231.
[6] 赵伟,耿岩玲,崔莉,等.牡丹花黄酮类化学成分研究[J].中国现代中药,2016,18(3):303-306.
[7] 戴晓庆,豪汪,叶文才,等.维药香青兰叶的化学成分研究[J].药学与临床研究,2018,18(3):267-268.
[8] 曹莉,黄多临,陈华.大叶紫珠的化学成分研究[J].中国现代中药,2014,16(9):733-734.
[9] 海姜,满文静,柳杨,等.苍耳子中咖啡酰奎宁酸类化学成分的研究[J].长春中医药大学学报,2019,35(1):104-107.
[10] CHEN F Z,XIANG Q X,LI S H.Chemical constituents in the leaves of Alsophila spinulosa[J].Acta Bot Borea Occident Sin,2008,28(6):1246-1249.
[11] 马子玉,卢青秀,年贺凤,等.多花山竹子果实化学成分研究[J].中草药,2019,50(1):17-21.
[12] 董岩,南叶飞,蔡雪刁,等.β-(3,4-二羟基苯基)-α-羟基丙酸异丙酯/冰片酯合成研究[J].有机化学,2009,29(9):1466-1469.
[13] LI H,ZHOU C,PAN Y,et al.Evaluation of Antiviral Activity of Compounds Isolated from Ranunculus sieboldii and Ranunculus sceleratus[J].Planta Med,2005,71(12):1128-1133.
[14] KIMA Y H.Chrysoeriol isolated from the leaves of Eurya ciliata stimulate-sproliferation and differentiation of osteoblastic MC3T3-E1 cells[J].J Asian Nat Prod Res,2009,11(9):817-823.
[15] 林云良,邱明华,李忠荣,等.黄杨中的非生物碱化学成分[J].云南植物研究,2006,28(4):429-432.
[16] SEMWAL R B,SEMWAL D K,SEMWAL R,et al.Chemical constituents from the stem bark of Symplocos paniculata Thunb.with antimicrobial,analgesic and anti-inflammatory activities[J].J Ethnopharmacol,2011,135(1):78-87.
[17] 叶素梅.芹菜素对黄嘌呤氧化酶活性的抑制机理研究[J].食品研究与开发,2018,39(21):67-71.
[18] 谢杨春,邓永诚,田黎.芹菜素诱导膀胱癌5637细胞凋亡的机制研究[J].中国民族民间医药,2016,25(13):25-28.
[19] 邱文慧,沈建昌,林丽纯.穗花蛇菰的抗发炎成分研究[J].药物食品分析,2011,19(4):122-128.
[20] 丁永桢,温嘉泳,赖宝龙,等.咖啡酸抗人乳头瘤病毒感染的研究[J].病毒学报,2018,34(3):287-295.
[21] 彭钰芳,邹文韬,许传莲.杭白菊中黄酮类化合物及其抑制人源肿瘤细胞增殖活性的研究[J].中国药学杂志,2010,45(19):1454-1459.
[22] 龚春燕,张道敬,魏鸿刚,等.多粘类芽孢杆菌HY96-2发酵液化学成分研究[J].天然产物研究与开发,2009,21(3):379-381,387.
[23] ITHO A,SADANARI H,TAKEMOTO M,et al.Tricin inhibits CCL5 induction required for the efficient growth of human cytomegalovirus[J].Microbiol Immunol,2018,62(5):341-347.
[24] YANG Y,ZHOU X,XIAO M,et al.Discovery of chrysoeriol,a PI3K-AKT-mTOR pathway inhibitor with potent antitumor activity against human multiple myeloma cells in vitro[J].J Huazhong Univ Sci Technolog Med Sci,2010,30(6):734-740.
[25] WANG M,CUI H X,SUN C,et al.Effect of lupeol on migration and invasion of human breast cancer MDA-MB-231 cells and its mechanism[J].Acta Pharm Sin,2016,51(4):558.