芜湖地区猪瘟、猪口蹄疫、高致病性猪蓝耳病抗体水平监测与分析
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摘要
目的:为评估芜湖地区猪瘟、猪口蹄疫、高致病性猪蓝耳病免疫效果,对2013～2016年这3种疫病血清抗体水平进行监测,为芜湖地区综合防控相关疫病提供基础依据。方法:对芜湖地区部分规模猪场和散养户进行血清学调查,共采集血清样品1 038份,使用间接ELISA(酶联免疫吸附试验)进行监测。结果:(1)猪瘟血清抗体平均阳性率为91.9%,芜湖四区四县中血清抗体阳性率最高的为弋江区,最低为南陵县,规模养殖户的血清抗体阳性率略低于散养户。(2)猪口蹄疫血清抗体平均阳性率为83.9%,芜湖四区四县中血清抗体阳性率最高的为三山区,最低为繁昌县,规模养殖户血清抗体平均阳性率高于散养户。(3)高致病性猪蓝耳病血清抗体平均阳性率为65.1%,低于国家免疫要求,需加强针对性免疫措施。结论:监测结果表明,猪瘟、猪口蹄疫血清抗体水平符合国家要求,高致病性猪蓝耳病血清抗体水平低于国家要求。
Objective:To evaluate the immune effect of classical swine fever,pig foot-and-mouth disease,high pathogenic porcine reproductive and respiratory syndrome in Wuhu region,serum antibody levels of pig diseases above in Wuhu region were monitored from 2013 to 2016,which provide a basis of prevention and control of related diseases in Wuhu region.Methods:Serological investigation were carried out on some pig farms and individual farmers in Wuhu area.1038 serum samples were collected and monitored by indirect ELISA(enzyme-linked immunosorbent assay).Results:(1)The average positive rate of serum antibody of classical swine fever was 91.9%,the highest positive rate of serum antibody was in Yijiang district and the lowest was in Nanling county.The positive rate of serum antibody in large-scale farmers was slightly lower than that in bulk farmers.(2)The average positive rate of foot-and-mouth disease of pigs' serum antibody was 83.9%.The highest positive rate of serum antibody was in Sanshan district,and the lowest was in Fanchang county.(3)The average positive rate of serum antibody for high pathogenic porcine reproductive and respiratory syndrome was 65.1%,lower than the national immunization requirements.Targeted immunization measures should be strengthened.Conclusion:The monitoring results showed that the serum antibody levels of swine fever and foot-and-mouth disease met the national requirements,and the serum antibody levels of high pathogenic porcine reproductive and respiratory syndrome were lower than the national requirements.
Objective:To evaluate the immune effect of classical swine fever,pig foot-and-mouth disease,high pathogenic porcine reproductive and respiratory syndrome in Wuhu region,serum antibody levels of pig diseases above in Wuhu region were monitored from 2013 to 2016,which provide a basis of prevention and control of related diseases in Wuhu region.Methods:Serological investigation were carried out on some pig farms and individual farmers in Wuhu area.1038 serum samples were collected and monitored by indirect ELISA(enzyme-linked immunosorbent assay).Results:(1)The average positive rate of serum antibody of classical swine fever was 91.9%,the highest positive rate of serum antibody was in Yijiang district and the lowest was in Nanling county.The positive rate of serum antibody in large-scale farmers was slightly lower than that in bulk farmers.(2)The average positive rate of foot-and-mouth disease of pigs' serum antibody was 83.9%.The highest positive rate of serum antibody was in Sanshan district,and the lowest was in Fanchang county.(3)The average positive rate of serum antibody for high pathogenic porcine reproductive and respiratory syndrome was 65.1%,lower than the national immunization requirements.Targeted immunization measures should be strengthened.Conclusion:The monitoring results showed that the serum antibody levels of swine fever and foot-and-mouth disease met the national requirements,and the serum antibody levels of high pathogenic porcine reproductive and respiratory syndrome were lower than the national requirements.
引文
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[9]郝永清,王潇,张爱荣,等.内蒙古猪瘟流行病学调查及猪瘟病毒的分离[J].内蒙古农业大学学报(自然科学版),2004(4):1-4.
[10]金仕强,杨丁,刘丽,等.2017年宜宾市猪口蹄疫,猪瘟,高致病性猪蓝耳病免疫抗体情况调查[J].四川畜牧兽医,2018,45(7):20-21.
[11]贺绍君,刘德义,李静,等.环境应激对生长后期肉鸡免疫器官组织结构的影响[J].安徽科技学院学报,2013,27(4):5-9.
[12]丁梦蝶,陈斌,裴超信,等.四川省2017年高致病性猪蓝耳病和猪瘟免疫抗体监测与分析[J].畜禽业,2018,3(346):17-18.
[2]崔筱雨,隋树丛,苏炳娟,等.血常规检测技术在猪群健康监测中的应用[J].黑龙江畜牧兽医,2018(2):86-88.
[3]聂奎,李竹青,曾政,等.川渝部分地区育肥猪伪狂犬病血清流行病学调查及危险性因素[J].西南大学学报(自然科学版),2013,35(9):6-10.
[4]陈冬梅,李晓霞,孙剑峰,等.2015-2017年甘肃省猪瘟抗体监测及免疫效果分析[J].国外畜牧学,2018,38(4):67-70.
[5]李建玲,赵庆,蔡扩军,等.猪瘟,高致病性猪蓝耳病和口蹄疫母源抗体的测定及3种疫苗首日免疫日龄的确定[J].中国兽医杂志,2018,54(1):34-36.
[6]张冬冬,季慕寅,徐迪,等.芜湖市规模鸡场禽流感抗体水平的监测与分析[J].安徽科技学院学报,2016,30(1):25-29.
[7]中华人们共和国农业农村部.2018年国家动物疫病强制免疫计划[J].北方牧业,2018(3):32.
[8]洪厚成,魏忠云,杜丽飞,等.猪高致病性蓝耳病的诊断及其防治措施[J].中国动物检疫,2011,28(1):70-72.
[9]郝永清,王潇,张爱荣,等.内蒙古猪瘟流行病学调查及猪瘟病毒的分离[J].内蒙古农业大学学报(自然科学版),2004(4):1-4.
[10]金仕强,杨丁,刘丽,等.2017年宜宾市猪口蹄疫,猪瘟,高致病性猪蓝耳病免疫抗体情况调查[J].四川畜牧兽医,2018,45(7):20-21.
[11]贺绍君,刘德义,李静,等.环境应激对生长后期肉鸡免疫器官组织结构的影响[J].安徽科技学院学报,2013,27(4):5-9.
[12]丁梦蝶,陈斌,裴超信,等.四川省2017年高致病性猪蓝耳病和猪瘟免疫抗体监测与分析[J].畜禽业,2018,3(346):17-18.