HPLC法测定东革阿里中宽缨酮的含量
|
摘要
目的:测定东革阿里中宽缨酮含量。方法:采用Waters Sunfire C18(4.6×250mm,5μm)色谱柱,乙腈-0.1%甲酸为流动相梯度洗脱,流速1.0mL/min,检测波长:238nm,柱温40℃。结果:宽缨酮在线性范围1.272～101.72μg·mL-1(r=0.9998)内具有良好的线性关系,平均回收率分别为98.87%(n=9),日内精密度RSD为0.46%。结论:本法操作简便,结果稳定,重现性好,可作为东革阿里中宽缨酮的质量控制方法。
AIM:To establish an HPLC method for quantitative determining of eurycomanone in Eurycoma longifolia Jack.METHODS:Separation was accomplished on the Waters Sunfire C18(4.6×250 mm,5 μm)column,with acetonitrile-0.1%formic acid as mobile phase in gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was set at238 nm. The column temperature was set at 40℃. RESULTS:The method offered good linearity 1.272～101.72μg·mL-1 for eurycomanone(r=0.9998). The average recovery rate was 98.87%(n=9)and the intra-day precision RSD was 0.46%.CONCLUSION:Themethodwasconvenient,accurateandpracticable,andcanbeusedforqualitycontrolofthispreparation.
AIM:To establish an HPLC method for quantitative determining of eurycomanone in Eurycoma longifolia Jack.METHODS:Separation was accomplished on the Waters Sunfire C18(4.6×250 mm,5 μm)column,with acetonitrile-0.1%formic acid as mobile phase in gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was set at238 nm. The column temperature was set at 40℃. RESULTS:The method offered good linearity 1.272～101.72μg·mL-1 for eurycomanone(r=0.9998). The average recovery rate was 98.87%(n=9)and the intra-day precision RSD was 0.46%.CONCLUSION:Themethodwasconvenient,accurateandpracticable,andcanbeusedforqualitycontrolofthispreparation.
引文
[1] Al-SALAHI O S A,JI D,MAJID AMSA,et a1.Anti-Tumor Activity of Eurycoma longifolia Root Extracts against K-562 Cell Line:in vitro and in vivo Study[J]. PLoS One,2014,9(1):e83818.
[2] AL-ADHROEY A H,NOR Z M,AL-MEKHLAFI H M,et a1. Ethnobotanical study on some Malaysian anti-malarial plants:a community based survey[J]. J Ethnopharmacol,2010,132(1):362-364.
[3] KOTIRUM S,ISMAIL S B,CHAIYAKUNAPRUK N. Efficacy of Tongkat Ali(Eurycoma longifolia)on erectile function improvement:systematic review and Meta-analysis of randomized controlled trials[J]. Complement Ther Med,2015,23(5):693-698.
[4] RALF R H,RUXIANG W,SUSAN H. B,et a1. Tongkat ali as a potential herbal supplement for physically active male and female seniors-A Pilot study[J]. Phytother Res,2014,28:544-550.
[5] ANG H H,CHEANG H S. Studies on the anxiolytic activity of Eurycoma longifolia Jack roots in mice[J]. Jpn J Pharmacol,1999,79(4):497-500.
[6] SHUID A N,ABU BAKAR M F,ABDUL SHUKOR T A,et al. The anti-osteoporotic effect of Eurycoma Longifolia in aged orchidectomised rat model[J].Aging Male,2011,14(3):150-154.
[7] YOUNG M H,SANG-UK W,MIN S C. Anti-inflammatory and analgesic effects of Eurycoma longifolia extracts[J].Arch Pharm Res,2016,39:421-428.
[8] ZAKIA K,CHEW S W,IRSHAD U H B. Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia Jack(Tongkat Ali)[J]. J King Sand Univ Sci,2015,27:23-30.
[9] SURATWADEE J,VILAI S,AKIRA M. In vitro anti-tumor promoting and anti-parasitic activities of the quassinoids from Eurycoma longifolia,a medicinal plant in Southeast Asia[J]. J Ethnopharmacol,2002,82:55-58.
[10] ANNIE G,NAOKO S,AZREENA B A.Immunomodulation in middle-aged humans via the ingestion of physta standardized root water extract of Eurycoma longifolia Jack-a randomized,double-blind,placebo-controlled,parallel study[J]. Phytother Res,2016,30:627-635.
[11] OMAR S A A-S,CHAN K-L,AMIN M S A M,et a1.Antiangiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function[J]. Microvasc Res,2013,90:30-39.
[12] RAFIDAH H,AZIMAHTOL H L P,MEENAKSHII N.Screening for antihyperglycaemic activity in several local herbs of Malaysia[J]. J Ethnopharmacol,2004,95:205-208.
[13] Rajeev B,Karim A A. Tongkat Ali(Eurycoma longifolia Jack):A review on its ethnobotany and pharmacological importance[J]. Fitoterapia,2010,81:669-679.
[14] TONG K L,CHAN K L,ABUBAKAR S,et a1.The in vitro and in vivo Anti-Cancer Activities of a Standardized Quassinoids Composition from Eurycoma longifolia on LNCaP Human Prostate Cancer Cells[J]. PLoS One,2015,10(3):e0121752.
[15] BIN-SENG L,PRASHANTA K D,KIT-LAM C. Acute,reproductive toxicity and two-generation teratology studies of a standardized quassinoidrich extract of Eurycoma longifolia Jack in Sprague-dawley rats[J]. Phytother Res,2014,28:1022-1029.
[16] BIN-SENG L,PRASHANTA K D,KIT-LAM C. Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic-pituitary-gonadal axis[J]. J Ethnopharmacol,2013,145:706-714.
[2] AL-ADHROEY A H,NOR Z M,AL-MEKHLAFI H M,et a1. Ethnobotanical study on some Malaysian anti-malarial plants:a community based survey[J]. J Ethnopharmacol,2010,132(1):362-364.
[3] KOTIRUM S,ISMAIL S B,CHAIYAKUNAPRUK N. Efficacy of Tongkat Ali(Eurycoma longifolia)on erectile function improvement:systematic review and Meta-analysis of randomized controlled trials[J]. Complement Ther Med,2015,23(5):693-698.
[4] RALF R H,RUXIANG W,SUSAN H. B,et a1. Tongkat ali as a potential herbal supplement for physically active male and female seniors-A Pilot study[J]. Phytother Res,2014,28:544-550.
[5] ANG H H,CHEANG H S. Studies on the anxiolytic activity of Eurycoma longifolia Jack roots in mice[J]. Jpn J Pharmacol,1999,79(4):497-500.
[6] SHUID A N,ABU BAKAR M F,ABDUL SHUKOR T A,et al. The anti-osteoporotic effect of Eurycoma Longifolia in aged orchidectomised rat model[J].Aging Male,2011,14(3):150-154.
[7] YOUNG M H,SANG-UK W,MIN S C. Anti-inflammatory and analgesic effects of Eurycoma longifolia extracts[J].Arch Pharm Res,2016,39:421-428.
[8] ZAKIA K,CHEW S W,IRSHAD U H B. Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia Jack(Tongkat Ali)[J]. J King Sand Univ Sci,2015,27:23-30.
[9] SURATWADEE J,VILAI S,AKIRA M. In vitro anti-tumor promoting and anti-parasitic activities of the quassinoids from Eurycoma longifolia,a medicinal plant in Southeast Asia[J]. J Ethnopharmacol,2002,82:55-58.
[10] ANNIE G,NAOKO S,AZREENA B A.Immunomodulation in middle-aged humans via the ingestion of physta standardized root water extract of Eurycoma longifolia Jack-a randomized,double-blind,placebo-controlled,parallel study[J]. Phytother Res,2016,30:627-635.
[11] OMAR S A A-S,CHAN K-L,AMIN M S A M,et a1.Antiangiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function[J]. Microvasc Res,2013,90:30-39.
[12] RAFIDAH H,AZIMAHTOL H L P,MEENAKSHII N.Screening for antihyperglycaemic activity in several local herbs of Malaysia[J]. J Ethnopharmacol,2004,95:205-208.
[13] Rajeev B,Karim A A. Tongkat Ali(Eurycoma longifolia Jack):A review on its ethnobotany and pharmacological importance[J]. Fitoterapia,2010,81:669-679.
[14] TONG K L,CHAN K L,ABUBAKAR S,et a1.The in vitro and in vivo Anti-Cancer Activities of a Standardized Quassinoids Composition from Eurycoma longifolia on LNCaP Human Prostate Cancer Cells[J]. PLoS One,2015,10(3):e0121752.
[15] BIN-SENG L,PRASHANTA K D,KIT-LAM C. Acute,reproductive toxicity and two-generation teratology studies of a standardized quassinoidrich extract of Eurycoma longifolia Jack in Sprague-dawley rats[J]. Phytother Res,2014,28:1022-1029.
[16] BIN-SENG L,PRASHANTA K D,KIT-LAM C. Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic-pituitary-gonadal axis[J]. J Ethnopharmacol,2013,145:706-714.