虎杖苷转化菌的筛选及所产酶的性质研究
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摘要
利用专一性底物法,以虎杖水提液为初始培养基,经过初筛复筛获得转化菌。根据菌落形态特征对菌株进行初步鉴定,对转化菌产酶的酶学性质进行研究。结果表明,通过筛选得到1株特异性转化虎杖苷为白藜芦醇的菌株,经形态学鉴定为青霉菌。该菌产的酶最适p H值为5. 0,最适温度为60℃; Fe2+、Na+和Ca2+对酶活力有激活作用;虎杖苷是该酶理想的水解作用底物。利用该菌产的酶液转化虎杖原料,虎杖苷的转化率最高可达到100%。
引文
[1]刘盛楠,邵淑丽,隋文静,等.白藜芦醇诱导肺癌A549细胞凋亡[J].基因组学与应用生物学,2015,34(4):685-691.
[2]ztürka E,Arslana A K K,Yerera M B,et al. Resveratrol and diabetes:a critical review of clinical studies[J]. Biomedicine&Pharmacotherapy,2017,95:230-234.
[3]Harper C,Pate B B. Resveratrol suppresses prostate cancer progression in transgenic mice[J]. Carcinogenesis,2007,28(9):1946-1953.
[4]董雯,王蓉.白藜芦醇诱导细胞自噬在神经退行性疾病进展中的作用[J].药学学报,2016,51(1):18-22.
[5]Patel K R,Brown V A,Jones D J,et al. Clinical pharmacology of resveratrol and its metabolites in colorectal cancer patients[J].Cancer Res,2010,70(19):7392-7399.
[6]Alejandra A F,Helder D S,Robert S F,et al. Formation stability and antioxidant activity of food-grade multilayer emulsions containing resveratrol[J]. Food Hydrocolloids,2017,71:207-215.
[7]Wang W B,Lai H C,Hsueh P R,et al. Inhibition of swarming and virulence factor expression in Proteus mirabilis by resveratrol[J]. J Med Microbiol,2006,55(10):1313-1321.
[8]Tung B T,Rodriguez-Bies E,Talero E,et al. Anti-inflammatory effect of resveratrol in old mice liver[J]. Exp Gerontol,2015,64:1-7.
[9]李先宽,李赫宇,李帅,等.白藜芦醇研究进展[J].中草药,2016,47(14):2568-2578.
[10]张文根,杨赛刚,李波,等.中国寥科植物化学成分研究进展[J].现代生物医学进展,2008,8(2):393-396.
[11]周建军,张宏杰,杨培君.不同地区虎杖中白藜芦醇苷及苷元的含量比较[J].中药材,2005,28(l):31-33.
[12]任建伟.β-葡萄糖苷酶高产菌株选育及发酵条件优化[D].杭州:浙江大学,2015.
[13]田天丽.微生物发酵转化虎杖的研究[D].成都:四川大学,2007:6-7.
[14]叶秋雄,黄苇.白藜芦醇苷的稳定性研究[J].食品工业科技,2011,32(4):155-157.
[15]王辉.微生物转化虎杖中白藜芦醇苷及其产物的分离纯化[D].大连:大连理工大学,2008:29-30.
[16]陈蓉蓉,姜华,浦含林.酶解法制备白藜芦醇的工艺优化[J].食品工程,2011(11):151-154.
[17]孙诗妻,张笑笛,宋字.葡萄中白藜芦醇合成酶基因的克隆及其转化黄芪研究[J].江苏农业科学,2017,45(9):38-41.
[2]ztürka E,Arslana A K K,Yerera M B,et al. Resveratrol and diabetes:a critical review of clinical studies[J]. Biomedicine&Pharmacotherapy,2017,95:230-234.
[3]Harper C,Pate B B. Resveratrol suppresses prostate cancer progression in transgenic mice[J]. Carcinogenesis,2007,28(9):1946-1953.
[4]董雯,王蓉.白藜芦醇诱导细胞自噬在神经退行性疾病进展中的作用[J].药学学报,2016,51(1):18-22.
[5]Patel K R,Brown V A,Jones D J,et al. Clinical pharmacology of resveratrol and its metabolites in colorectal cancer patients[J].Cancer Res,2010,70(19):7392-7399.
[6]Alejandra A F,Helder D S,Robert S F,et al. Formation stability and antioxidant activity of food-grade multilayer emulsions containing resveratrol[J]. Food Hydrocolloids,2017,71:207-215.
[7]Wang W B,Lai H C,Hsueh P R,et al. Inhibition of swarming and virulence factor expression in Proteus mirabilis by resveratrol[J]. J Med Microbiol,2006,55(10):1313-1321.
[8]Tung B T,Rodriguez-Bies E,Talero E,et al. Anti-inflammatory effect of resveratrol in old mice liver[J]. Exp Gerontol,2015,64:1-7.
[9]李先宽,李赫宇,李帅,等.白藜芦醇研究进展[J].中草药,2016,47(14):2568-2578.
[10]张文根,杨赛刚,李波,等.中国寥科植物化学成分研究进展[J].现代生物医学进展,2008,8(2):393-396.
[11]周建军,张宏杰,杨培君.不同地区虎杖中白藜芦醇苷及苷元的含量比较[J].中药材,2005,28(l):31-33.
[12]任建伟.β-葡萄糖苷酶高产菌株选育及发酵条件优化[D].杭州:浙江大学,2015.
[13]田天丽.微生物发酵转化虎杖的研究[D].成都:四川大学,2007:6-7.
[14]叶秋雄,黄苇.白藜芦醇苷的稳定性研究[J].食品工业科技,2011,32(4):155-157.
[15]王辉.微生物转化虎杖中白藜芦醇苷及其产物的分离纯化[D].大连:大连理工大学,2008:29-30.
[16]陈蓉蓉,姜华,浦含林.酶解法制备白藜芦醇的工艺优化[J].食品工程,2011(11):151-154.
[17]孙诗妻,张笑笛,宋字.葡萄中白藜芦醇合成酶基因的克隆及其转化黄芪研究[J].江苏农业科学,2017,45(9):38-41.