正交试验法优选肺炎合剂提取工艺
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摘要
目的优选肺炎合剂的提取工艺条件。方法以盐酸麻黄碱和虎杖苷含量及浸出液浓缩后的相对密度所构成的综合评分为评定指标,采用L_9(3~4)正交试验法,考察加水量、浸泡时间、提取时间、提取次数4个因素对水提取工艺的影响,并进行验证试验。结果最优提取工艺为加8倍量水,浸泡90/120 min,提取3次,每次提取1 h。结论以优选提取工艺制备的肺炎合剂中盐酸麻黄碱、虎杖苷含量高,溶液相对密度大,且稳定性、重复性好。
Objective To optimize the conditions of the extraction process for Feiyan Mixture. Methods With the comprehensive scores consisting of the content ephedrine hydrochloride and polydatin and the relative density of evaporated lixivium after concentration as the evaluation indexes, L_9(3~4) orthogonal test was used to investigate the effects of water addition, soaking time, extraction time and extraction times on the water extraction process, and validation test was carried out. Results The optimum extraction process was as follows : adding 8 times of water, soaking for 90/120 min,extracting for 3 times with 1 h each time. Conclusion In the Feiyan Mixture prepared by the optimized extraction process,the content of ephedrine hydrochloride and polydatin is high,the relative density of the solution is high,the stability and repeatability are good.
Objective To optimize the conditions of the extraction process for Feiyan Mixture. Methods With the comprehensive scores consisting of the content ephedrine hydrochloride and polydatin and the relative density of evaporated lixivium after concentration as the evaluation indexes, L_9(3~4) orthogonal test was used to investigate the effects of water addition, soaking time, extraction time and extraction times on the water extraction process, and validation test was carried out. Results The optimum extraction process was as follows : adding 8 times of water, soaking for 90/120 min,extracting for 3 times with 1 h each time. Conclusion In the Feiyan Mixture prepared by the optimized extraction process,the content of ephedrine hydrochloride and polydatin is high,the relative density of the solution is high,the stability and repeatability are good.
引文
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[2]贾越光,丁志英,张越,等,正交试验筛选北五味子保肝活性成分的提取工艺研究[J].中国生化药物杂志,2015,35(1):151-155.
[3]林淑贞,李美霞,罗春燕,正交设计优化桂枝和营消风合剂的制备工艺[J].海峡药学,2015,27(12):18-20.
[4]田杰,王艳平,宁娱,等,正交试验法优选强力五虎合剂的水提取工艺[J].中国现代中药,2016,6(18):789-792.
[5]马玉,肖禾,焦小珂,等.小儿解表止咳口服液中盐酸麻黄碱含量测定[J].中国药业,2014,23(6):37-38.
[6]魏萍,张亚锋,王苑桃.高效液相色谱法测定定喘止咳胶囊中盐酸麻黄碱含量[J].中国药业,2012,21(17):22-23.
[7]周红玲,石雕.高效液相色谱法测定复方咳喘颗粒中盐酸麻黄碱的含量[J].湖南中医药大学学报,2014,34(8):21-23.
[8]刘建华,刘文炜,高玉琼,等. HPLC测定祛痰平喘胶囊中盐酸麻黄碱的含量[J].中国实验方剂学杂志,2011,17(9):93-94.
[9]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:209,320.
[10]徐剑,张永萍.苗药痔康宁胶囊中大黄素和虎杖苷的含量测定[J].贵阳中医学院学报,2011,33(1):84-86.
[11]杨海燕,张成.高效液相色谱法测定朱砂七中虎杖苷含量[J].中国药业,2011,20(10):29-30.
[12]田文涛,杨茂文,杨金琰,等.高效液相色谱法测定复方伸筋片中虎杖苷的含量[J].中医药师,2013,17(3):522-523.
[13]张海欢,缪红. HPLC法测定疏风解毒胶囊中虎杖苷[J].中成药,2013,35(17):2281-2283.
[14]车彦忠,王培智,陈红英,等. HPLC测定护宁肝中虎杖苷的含量[J].中医临床研究,2013,5(19):27-29.
[15]滕海英,祝国强,黄平,等.正交试验设计实例分析[J].药学服务与研究,2008,8(1):75-76.
[16]齐辉,张勉,王峥涛. HPLC同时测定虎杖中4种成分的含量[J].中国中药杂志,2006,31(23):2003-2005.
[17]王健.小儿肾炎颗粒质量标准研究[J].中成药,2007,29(12):附10.