miRNAs调控在中医虚证发生中的作用
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摘要
中医虚证分为脏腑虚证、阴阳虚证、气血津液虚证等。其发病机制的影响因素广泛,涉及到神经、免疫、内分泌等多个系统。miRNA(microRNA,小RNA)在个体发育、细胞增殖分化、凋亡、代谢、神经等多个生理过程中产生重要作用,控制着多种疾病的发生发展进程。大量研究表明,miRNAs也是中医虚证发生的重要调控机制之一,在中医虚证的发生中发挥着重要作用。结合miRNA的主要功能,从脏腑虚证、气血津液虚证、阴阳虚证等方面,对miRNAs调控与中医虚证发生的相关性作一综述。
TCM deficiency syndrome is divided into Zang-fu deficiency syndrome,Yin-Yang deficiency syndrome, Qi-blood and fluid deficiency syndrome and so on. Its pathogenesis is affected by a wide range of factors, including nerve, immune, endocrine and other systems. MiRNA-microRNAs(small RNAs) play an important role in many physiological processes, such as ontogeny, cell proliferation, differentiation, apoptosis, metabolism, nerveand so on. They control the occurrence and development of many diseases. A large number of studies show that miRNAs are also one of the important regulatory mechanisms of deficiency syndrome in TCM, and play an important role in the occurrence of deficiency syndrome in TCM.This article, based on the main functions of miRNA, summarizes the correlation between the regulation of miRNAs and the deficiency syndrome in TCM from the aspects of the deficiency of Zang-fu, the deficiency of Qi-blood and the deficiency of Yin-Yang.
TCM deficiency syndrome is divided into Zang-fu deficiency syndrome,Yin-Yang deficiency syndrome, Qi-blood and fluid deficiency syndrome and so on. Its pathogenesis is affected by a wide range of factors, including nerve, immune, endocrine and other systems. MiRNA-microRNAs(small RNAs) play an important role in many physiological processes, such as ontogeny, cell proliferation, differentiation, apoptosis, metabolism, nerveand so on. They control the occurrence and development of many diseases. A large number of studies show that miRNAs are also one of the important regulatory mechanisms of deficiency syndrome in TCM, and play an important role in the occurrence of deficiency syndrome in TCM.This article, based on the main functions of miRNA, summarizes the correlation between the regulation of miRNAs and the deficiency syndrome in TCM from the aspects of the deficiency of Zang-fu, the deficiency of Qi-blood and the deficiency of Yin-Yang.
引文
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[21] 崔欢.结直肠癌术后气虚证患者证素分布特点及其与血浆miR-133a、miR-152的关系[D].福州:福建中医药大学,2017.
[22] Yang Weili,ChenJi,MengYuhong,et al.Novel Targets for Treating Ischemia-Reperfusion Injury in the Liver[J] .Int J MolSci,2018,19(5).
[23] Zohora Fatema,Bidad Katayoon,Pourpak Zahra,et al.Biological and Immunological Aspects of Iron Deficiency Anemia in Cancer Development:A Narrative Review[J].Nutr Cancer,2018.
[24] 李峻,陈劼,刘月皎,等.血复生浸膏结合辨证类方对慢性再生障碍性贫血患者外周血miRNA、淋巴细胞亚群及细胞因子影响的临床观察[J].上海中医药杂志,2017,51(10):42-47.
[2] 申维玺.中医虚证与食管癌、肺癌生物学行为关系及肺阴虚证本质的研究[D].北京:中国协和医科大学,1999.
[3] Uddin A,Chakraborty S.Role of miRNAs in lung cancer[J].J Cell Physiol,2018:1-10.
[4] Gregory Richard I,Yan Kai-Ping,Amuthan Govindasamy,et al.The Microprocessor complex mediates the genesis of microRNAs[J].Nature,2004,432(7014):235-240.
[5] Long Hai,WangXin,ChenYongjian,et al.Dysregulation of microRNAs in autoimmune diseases:pathogenesis,biomarkers and potential therapeutic targets[J].Cancer Lett.,2018.
[6] Mehta A,Baltimore D.MicroRNAs as regulatory elements in immune system logic[J].Nature reviews.Immunology,2016,28,16(5):279-294.
[7] 梁文娜,李西海,李灿东.血清microRNA与绝经后骨质疏松症肾虚证的关系[J].中医正骨,2017,29(10):53-56.
[8] 孙玉文.基于“肾为先天之本”2型糖尿病肾虚证的miRNA研究[D].成都:成都中医药大学,2013.
[9] 陶文华.基于miR-29b调节SP1/IL-10对D-IBS合并2型糖尿病肝郁脾虚证的分子生物学研究[D].扬州:扬州大学,2016.
[10] 原宁.结直肠腺瘤中医虚实证候miRNA表达谱的研究[D].广州:广州中医药大学,2012.
[11] 杨泽民,陈滢宇,杨小蓉,等.血脂异常症脾虚证患者血清microRNA差异表达谱生物信息学分析[J/OL].中国中西医结合杂志:1-6[2018-02-08].http://kns.cnki.net.fd.mylibs.cn:808/kcms/detail/11.2787.R.20171229.1342.008.html.
[12] 杨阳,罗义.MicroRNAs与缺血性心脏病关系的研究进展[J].医学综述,2013,19(5):786-788.
[13] Zhang Li,Wu Yanli,Li Yumei,et al.Tanshinone IIA improves miR-133 expression through MAPK ERK1/2 pathway in hypoxic cardiac myocytes[J].Cell.Physiol.Biochem.,2012,30(4):843-852.
[14] 颜家渝,黄映红,曾洁萍.阴虚火旺型OLP差异表达miRNAs靶基因信号通路调控分析[J].中药与临床,2011,2(3):52-55.
[15] 袁雅琪.干燥综合征阴虚证患者中miR-17-92的表达与临床谱及中医证型的相关性研究[D].南京:南京中医药大学,2014.
[16] 武彦伶.阳虚、阴虚体质唾液miRNA表达谱分析[D].北京:北京中医药大学,2017.
[17] 杨飞,陈金妍,谭从娥,等.肾阳虚证患者外周血差异表达microRNAs筛选及功能预测[J].现代中西医结合杂志,2017,26(14):1483-1485,1490.
[18] 魏任雄,崔云,黄纪红,等.男性不育中医证型与精浆游离microRNA表达相关性研究[J].中国男科学杂志,2016,30(5):34-39,46.
[19] 许嗣立,崔珈铭,李炜弘,等.右归丸调控老龄肾阳虚免疫相关基因表达水平的研究[J].中华中医药学刊,2016,34(7):1609-1612.
[20] 周小军.气虚鼻咽癌特征性miRNA表达研究[C]//中华中医药学会耳鼻喉科分会 (China Association of Chinese Medicine Department of ENT branch)、 世界中联耳鼻喉口腔科专业委员会 (World Federation of Chinese Medicine Societies).中华中医药学会耳鼻喉科分会第二十三次学术年会、世界中联耳鼻喉口腔科专业委员会第九次学术年会论文集.中华中医药学会耳鼻喉科分会 (China Association of Chinese Medicine Department of ENT branch)、 世界中联耳鼻喉口腔科专业委员会 (World Federation of Chinese Medicine Societies),2017:1.
[21] 崔欢.结直肠癌术后气虚证患者证素分布特点及其与血浆miR-133a、miR-152的关系[D].福州:福建中医药大学,2017.
[22] Yang Weili,ChenJi,MengYuhong,et al.Novel Targets for Treating Ischemia-Reperfusion Injury in the Liver[J] .Int J MolSci,2018,19(5).
[23] Zohora Fatema,Bidad Katayoon,Pourpak Zahra,et al.Biological and Immunological Aspects of Iron Deficiency Anemia in Cancer Development:A Narrative Review[J].Nutr Cancer,2018.
[24] 李峻,陈劼,刘月皎,等.血复生浸膏结合辨证类方对慢性再生障碍性贫血患者外周血miRNA、淋巴细胞亚群及细胞因子影响的临床观察[J].上海中医药杂志,2017,51(10):42-47.