黄瓜枯萎病研究进展
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摘要
黄瓜枯萎病是一种由土壤传染,病原菌通过黄瓜的根或根颈部侵入,在维管束内寄生的系统性病害;是中国和世界范围内黄瓜生产中最具破坏性的疾病之一,因此对黄瓜枯萎病抗性育种的研究具有重要意义。从黄瓜枯萎病的病原菌的生物学特性、鉴定方法、黄瓜枯萎病的症状及病情分级等方面,分析了黄瓜枯萎病的致病机制、抗病机制和抗性遗传规律,总结了其农业防治、生物防治和化学防治等综合防治措施,以期为今后黄瓜枯萎病的抗性育种和科学防治提供理论依据。
Cucumber fusarium wilt is a systemic disease which is transmitted by soil. The pathogen Fusarium oxysporum(Schl.) F. sp cucumerinum Owen invades the root or root neck of cucumber and parasitizes in vascular bundles. As one of the most destructive diseases in cucumber production in China and the world, the research on cucumber fusarium wilt resistance breeding is of great significance. From the biological characteristics, identification methods, symptoms and disease classification of cucumber fusarium wilt, the pathogenic mechanism, resistance mechanism and resistance heredity of cucumber fusarium wilt are analyzed, and the comprehensive control measures such as agricultural control, biological control and chemical control are summarized in order to provide theoretical basis for resistance breeding and scientific control of cucumber fusarium wilt in the future.
Cucumber fusarium wilt is a systemic disease which is transmitted by soil. The pathogen Fusarium oxysporum(Schl.) F. sp cucumerinum Owen invades the root or root neck of cucumber and parasitizes in vascular bundles. As one of the most destructive diseases in cucumber production in China and the world, the research on cucumber fusarium wilt resistance breeding is of great significance. From the biological characteristics, identification methods, symptoms and disease classification of cucumber fusarium wilt, the pathogenic mechanism, resistance mechanism and resistance heredity of cucumber fusarium wilt are analyzed, and the comprehensive control measures such as agricultural control, biological control and chemical control are summarized in order to provide theoretical basis for resistance breeding and scientific control of cucumber fusarium wilt in the future.
引文
[1]刘永胜,王永,秦立金,等.FOC-RA-5诱导后黄瓜叶片内几种酶活性的变化[J].北方园艺,2016(6):1-5.
[2]Ye S F,Yu J Q,Peng Y H,et al.Incidence of Fusarium wilt in Cucumis sativus L.is promoted by cinnamic acid,an autotoxin in root exudates[J].Plant and Soil,2004,263(1):143-150.
[3]Hu J L,Lin X G,Wang J H,et al.Arbuscular mycorrhizal fungal inoculation enhances suppression of cucumber Fusarium wilt in greenhouse soils[J].Pedosphere,2010,20(5):586-593.
[4]郭晋云,胡晓峰,李勇,等.黄瓜枯萎病对黄瓜光合和水分生理特性的影响[J].南京农业大学学报,2011,34(01):79-83.
[5]杜宾.一株黄瓜枯萎病拮抗菌的鉴定及发酵培养基的优化[J].北方园艺,2017(3):132-137.
[6]沈凤瑞,吴萍,毛爱军,等.黄瓜抗枯萎病相关基因c DNA的分离及鉴定[J].华北农学报,2010,25(1):40-43.
[7]Ahn P,Chung H S,Lee Y H.Vegetative compatibility groups and pathogenicity among isolates of Fusarium oxysporum f.sp.cucumerinum[J].Plant Disease,1998,82(2):244-246.
[8]Yuan F,Zhang C L,Shen Q R.Alleviating effect of phenol compounds on cucumber fusarium wilt and mechanism[J].Agricultural Sciences in China,2003,2(6):647-652.
[9]Owen J H.Fusarium wilt of cucumber[J].Phytopathology,1955,45(8):435-439.
[10]Armsrong G M,Armstrong J K,Neter.Pathogenic races of the cucumber-wilt fusarium[J].Plant Disease Report,1987,62:824-828.
[11]翁祖信,徐新波,冯东昕.黄瓜枯萎病菌生理小种研究初报[J].中国蔬菜,1989(1):19-21.
[12]林中正,魏润洁,盛云泽,等.黄瓜枯萎病研究概述[J].北京农业,2011,(30):91-92.
[13]Zhang S S,Waseem R,Yang X M,et al.Control of fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer[J].Biology and Fertility of Soils,2008,44:1073-1080.
[14]King B C,Waxman K D,Nenni N V,et al.Arsenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi[J].Biotechnology for Biofuels,2011,4(1):4.
[15]Lionetti V,Métraux J P.Plant cell wall in pathogenesis,parasitism and symbiosis[J].Frontiers in Plant Science,2014,5(5):612.
[16]St?pień?,Wa?kiewicz A,Urbaniak M.Wildly growing asparagus(Asparagus officinalis L.)hosts pathogenic fusarium species and accumulates their mycotoxin[J].Microbial Ecology,2016,71(4):927-937.
[17]Singh V K,Singh H B,Upadhyay R S.Role of fusaric acid in the development of‘Fusarium wilt’symptoms in tomato:Physiological,biochemical and proteomic perspectives[J].Plant Physiology and Biochemistry,2017,118:320-332.
[18]Portal N,Soler A,Alphonsine P A M,et al.Nonspecific toxins as components of a host-specific culture filtrate from Fusarium oxysporum f.sp.cubense race 1[J].Plant Pathology,2018,67(2):467-476.
[19]Di-Pietro A,Garcia-Maceira F I,Meglecz E,et al.A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis[J].Molecular Microbiology,2001,39(5):1140-1152.
[20]López-Berges M S,Hera C,Sulyok M,et al.The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts[J].Molecular microbiology,2013,87(1):49-65.
[21]Michielse C B,Van Wijk R,Reijnen L,et al.Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f.sp.lycopersici through large-scale insertional mutagenesis[J].Genome Biology,2009,10(1):R4.
[22]韩金星,洪日新,周林,等.西瓜、黄瓜、甜瓜等瓜类枯萎病研究进展[J].中国瓜菜,2009,22(2):32-35.
[23]陈胜萍,付丽军,陈志,等.黄瓜抗枯萎病育种研究进展[J].河北农业科学,2011,15(11):50-53.
[24]马艳玲,吴凤芝,刘守伟.抗感枯萎病黄瓜品种的病理组织结构学研究[J].植物保护,2008,34(1):81-84.
[25]邹芳斌,司龙亭,李新,等.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究[J].华北农学报,2008,23(3):181-184.
[26]史娟,邱艳,王红玲,等.黄瓜几丁酶活性与其对枯萎病抗性的关系[J].宁夏农学院学报,2001,22(4):4-5.
[27]Netzer D,Niegro S,Galun F.A dominant gene conferring resistance to fusarium wilt in cucumber[J].Phytopathology,1977,77(4):525-527.
[28]Vakalounakis D J.Inheritance and linkage of resistance in cucumber line SMR-18 to races 1 and 2 of Fusarium oxysporu f.sp.cucumerium[J].Plant Pathology,1995,44:169-172.
[29]侯安福,尹彦.黄瓜枯萎病抗性遗传规律的研究[M].北京:科学出版社,1995.
[30]刘殿林,杨瑞环,哈玉洁.黄瓜抗枯萎病遗传特性的研究[J].天津农业科学,2003,9(2):33-35.
[31]张海英.黄瓜重要抗病基因的分子标记研究及遗传图谱的构建[D].北京:中国农业科学院,2006.
[32]张海英,张海霞,张峰,等.黄瓜枯萎病抗性基因的连锁分子标记[J].生物技术通报,2006(z1):320-322.[万方]
[33]刘缙,田花丽,王亚红,等.黄瓜转新型抗菌蛋白基因GNK2-1及其抗枯萎病的研究[J].植物学报,2010(4):411-418.
[34]沈凤瑞,吴萍,毛爱军,等.黄瓜抗枯萎病相关基因c DNA的分离及鉴定[J].华北农学报,2010,25(1):40-43.
[35]Zhang S P,Miao H,Sun R F,et al.Localization of a new gene for bitterness in cucumber[J].Journal of Heredity,2013,104:134-139.
[36]周红梅,董从娟,张海英,等.黄瓜抗枯萎病基因连锁分析和定位[J].分子植物育种,2015,13(9):1980-1986.
[37]毛爱军,张峰,张丽蓉,等.不同黄瓜材料对枯萎病的抗性评价[J].华北农学报,2008,23(2):214-216.
[38]WANG S N,YANG R X,LIU X,et al.Overcoming the autotoxicity of muskmelon by Trichoderma spp[J].Allelopathy Journal,2016,39(1):29-42.
[39]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[40]Raza W,Ling N,Zhang R F,et al.Success evaluation of the biological control of Fusarium wilts of cucumber,banana,and tomato since 2000 and future research strategies[J].Critical Reviews in Biotechnology,2017,37(2):202-212.
[41]Lu D D,Ma Z,Xu X H,et al.Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f.sp.Cucumerinum[J].Journal of Basic Microbiology,2016,56(8):929-933.
[42]余平,李文君,余丙华,等.黄瓜枯萎病与疫病的发生与防治[J].现代农业科技,2009(16):122,125.
[43]Pushpa K,Borkar G M,Patil D V.Studies on seed borne pathogens of pumpkin,cucumber,watermelon and mushmelon[J].Journal of Soils and Crops,1999,9(2):234-238.
[44]赵帅,杜春梅,田长彦.黄瓜枯萎病综合防治研究进展[J].中国农学通报,2014,30(7):254-259.
[2]Ye S F,Yu J Q,Peng Y H,et al.Incidence of Fusarium wilt in Cucumis sativus L.is promoted by cinnamic acid,an autotoxin in root exudates[J].Plant and Soil,2004,263(1):143-150.
[3]Hu J L,Lin X G,Wang J H,et al.Arbuscular mycorrhizal fungal inoculation enhances suppression of cucumber Fusarium wilt in greenhouse soils[J].Pedosphere,2010,20(5):586-593.
[4]郭晋云,胡晓峰,李勇,等.黄瓜枯萎病对黄瓜光合和水分生理特性的影响[J].南京农业大学学报,2011,34(01):79-83.
[5]杜宾.一株黄瓜枯萎病拮抗菌的鉴定及发酵培养基的优化[J].北方园艺,2017(3):132-137.
[6]沈凤瑞,吴萍,毛爱军,等.黄瓜抗枯萎病相关基因c DNA的分离及鉴定[J].华北农学报,2010,25(1):40-43.
[7]Ahn P,Chung H S,Lee Y H.Vegetative compatibility groups and pathogenicity among isolates of Fusarium oxysporum f.sp.cucumerinum[J].Plant Disease,1998,82(2):244-246.
[8]Yuan F,Zhang C L,Shen Q R.Alleviating effect of phenol compounds on cucumber fusarium wilt and mechanism[J].Agricultural Sciences in China,2003,2(6):647-652.
[9]Owen J H.Fusarium wilt of cucumber[J].Phytopathology,1955,45(8):435-439.
[10]Armsrong G M,Armstrong J K,Neter.Pathogenic races of the cucumber-wilt fusarium[J].Plant Disease Report,1987,62:824-828.
[11]翁祖信,徐新波,冯东昕.黄瓜枯萎病菌生理小种研究初报[J].中国蔬菜,1989(1):19-21.
[12]林中正,魏润洁,盛云泽,等.黄瓜枯萎病研究概述[J].北京农业,2011,(30):91-92.
[13]Zhang S S,Waseem R,Yang X M,et al.Control of fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer[J].Biology and Fertility of Soils,2008,44:1073-1080.
[14]King B C,Waxman K D,Nenni N V,et al.Arsenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi[J].Biotechnology for Biofuels,2011,4(1):4.
[15]Lionetti V,Métraux J P.Plant cell wall in pathogenesis,parasitism and symbiosis[J].Frontiers in Plant Science,2014,5(5):612.
[16]St?pień?,Wa?kiewicz A,Urbaniak M.Wildly growing asparagus(Asparagus officinalis L.)hosts pathogenic fusarium species and accumulates their mycotoxin[J].Microbial Ecology,2016,71(4):927-937.
[17]Singh V K,Singh H B,Upadhyay R S.Role of fusaric acid in the development of‘Fusarium wilt’symptoms in tomato:Physiological,biochemical and proteomic perspectives[J].Plant Physiology and Biochemistry,2017,118:320-332.
[18]Portal N,Soler A,Alphonsine P A M,et al.Nonspecific toxins as components of a host-specific culture filtrate from Fusarium oxysporum f.sp.cubense race 1[J].Plant Pathology,2018,67(2):467-476.
[19]Di-Pietro A,Garcia-Maceira F I,Meglecz E,et al.A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis[J].Molecular Microbiology,2001,39(5):1140-1152.
[20]López-Berges M S,Hera C,Sulyok M,et al.The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts[J].Molecular microbiology,2013,87(1):49-65.
[21]Michielse C B,Van Wijk R,Reijnen L,et al.Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f.sp.lycopersici through large-scale insertional mutagenesis[J].Genome Biology,2009,10(1):R4.
[22]韩金星,洪日新,周林,等.西瓜、黄瓜、甜瓜等瓜类枯萎病研究进展[J].中国瓜菜,2009,22(2):32-35.
[23]陈胜萍,付丽军,陈志,等.黄瓜抗枯萎病育种研究进展[J].河北农业科学,2011,15(11):50-53.
[24]马艳玲,吴凤芝,刘守伟.抗感枯萎病黄瓜品种的病理组织结构学研究[J].植物保护,2008,34(1):81-84.
[25]邹芳斌,司龙亭,李新,等.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究[J].华北农学报,2008,23(3):181-184.
[26]史娟,邱艳,王红玲,等.黄瓜几丁酶活性与其对枯萎病抗性的关系[J].宁夏农学院学报,2001,22(4):4-5.
[27]Netzer D,Niegro S,Galun F.A dominant gene conferring resistance to fusarium wilt in cucumber[J].Phytopathology,1977,77(4):525-527.
[28]Vakalounakis D J.Inheritance and linkage of resistance in cucumber line SMR-18 to races 1 and 2 of Fusarium oxysporu f.sp.cucumerium[J].Plant Pathology,1995,44:169-172.
[29]侯安福,尹彦.黄瓜枯萎病抗性遗传规律的研究[M].北京:科学出版社,1995.
[30]刘殿林,杨瑞环,哈玉洁.黄瓜抗枯萎病遗传特性的研究[J].天津农业科学,2003,9(2):33-35.
[31]张海英.黄瓜重要抗病基因的分子标记研究及遗传图谱的构建[D].北京:中国农业科学院,2006.
[32]张海英,张海霞,张峰,等.黄瓜枯萎病抗性基因的连锁分子标记[J].生物技术通报,2006(z1):320-322.[万方]
[33]刘缙,田花丽,王亚红,等.黄瓜转新型抗菌蛋白基因GNK2-1及其抗枯萎病的研究[J].植物学报,2010(4):411-418.
[34]沈凤瑞,吴萍,毛爱军,等.黄瓜抗枯萎病相关基因c DNA的分离及鉴定[J].华北农学报,2010,25(1):40-43.
[35]Zhang S P,Miao H,Sun R F,et al.Localization of a new gene for bitterness in cucumber[J].Journal of Heredity,2013,104:134-139.
[36]周红梅,董从娟,张海英,等.黄瓜抗枯萎病基因连锁分析和定位[J].分子植物育种,2015,13(9):1980-1986.
[37]毛爱军,张峰,张丽蓉,等.不同黄瓜材料对枯萎病的抗性评价[J].华北农学报,2008,23(2):214-216.
[38]WANG S N,YANG R X,LIU X,et al.Overcoming the autotoxicity of muskmelon by Trichoderma spp[J].Allelopathy Journal,2016,39(1):29-42.
[39]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[40]Raza W,Ling N,Zhang R F,et al.Success evaluation of the biological control of Fusarium wilts of cucumber,banana,and tomato since 2000 and future research strategies[J].Critical Reviews in Biotechnology,2017,37(2):202-212.
[41]Lu D D,Ma Z,Xu X H,et al.Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f.sp.Cucumerinum[J].Journal of Basic Microbiology,2016,56(8):929-933.
[42]余平,李文君,余丙华,等.黄瓜枯萎病与疫病的发生与防治[J].现代农业科技,2009(16):122,125.
[43]Pushpa K,Borkar G M,Patil D V.Studies on seed borne pathogens of pumpkin,cucumber,watermelon and mushmelon[J].Journal of Soils and Crops,1999,9(2):234-238.
[44]赵帅,杜春梅,田长彦.黄瓜枯萎病综合防治研究进展[J].中国农学通报,2014,30(7):254-259.