3种蓑藓属(Macromitrium)植物DNA C-值测定——兼论苔藓DNA C-值研究的生物学意义
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摘要
分别以小立碗藓(Physcomitrella patens)和水稻(日本晴亚种)(Oryza sativa spp.japonica)为标样,用流式细胞术分别测定了中华蓑藓(Macromirium cavaleriei)、钝叶蓑藓(M.japonicum)和缺齿蓑藓(M.gymnostomum)3种藓类植物的DNA C-值,由此建立了应用流式细胞术测定蓑藓属植物DNA C-值的方法,讨论了影响藓类植物DNA C-值测定结果的因素.最后讨论了苔藓植物DNA C-值变异式样、系统发育和分类学意义,以及今后有关藓类植物DNA C-值研究中值得关注的几个方向.
The DNA C-values of three Macromitrium species(including M.cavaleriei,M.japonicum and M.gymnostomum) were determined by flow cytometry by using Physcomitrella patens and Oryza sativa spp. japonica as standard samples,respectively.Thus the method of measuring DNA C-values of Macromitrium species by flow cytometry was established.The factors influencing the measuring result were also discussed.Finnally,variaiton patterns of bryophytes DNA C-values,their phylogenetic and taxonomic significances,and some interesting topics about DNA C-value research were remarked.
The DNA C-values of three Macromitrium species(including M.cavaleriei,M.japonicum and M.gymnostomum) were determined by flow cytometry by using Physcomitrella patens and Oryza sativa spp. japonica as standard samples,respectively.Thus the method of measuring DNA C-values of Macromitrium species by flow cytometry was established.The factors influencing the measuring result were also discussed.Finnally,variaiton patterns of bryophytes DNA C-values,their phylogenetic and taxonomic significances,and some interesting topics about DNA C-value research were remarked.
引文
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[2] FLAVELL R B.The molecular characterization and organization of plant chromosomal DNA sequences [J].Annual Review of Plant Physiology,1980,31:569-596.
[3] CAVALIER-SMITH T.Eukaryote gene numbers,non-coding DNA and genome size [M]//The Evolution of Genome Size.Chichester,Britain:John Wiley & Sons,1985:69-103.
[4] BENNETT M D,LEITCH L J.Nuclear DNA amounts in angiosperms:progress,problems and prospects [J].Annals of Botany,2005,95:45-90.
[5] 郭水良,周平,印丽萍,等.植物DNA C-值在种间和种内的变异及其生物学意义 [J].上海师范大学学报(自然科学版),2011,40(1):102-110.GUO S L,ZHOU P,YIN L P,et al.Interspecific and intraspecific variations of plant DNA C-values and its biological significance [J].Journal of Shanghai Normal University (Natural Sciences),2011,40(1):102-110.
[6] OHRI D,FRISCH R M,HANELT P.Evolution of genome size in Allium (Alliaceae) [J].Plant Systematics and Evolution,1998,210:57-86.
[7] BENNETT M D,BHANDOL P,LEITCH I J.Nuclear DNA amounts in angiosperms and their modern uses [J].Annals of Botany,2000,86:859-909.
[8] DOLE?ALOVA I,LEBEDA A,JANECEK J,et al.Variation in chromosome numbers and nuclear DNA contents in genetic resources of Lactuca L.species (Asteraceae) [J].Genetic Resources and Crop Evoluion,2002,49:383-395.
[9] KRAAIJEVELD K.Genome size and species diversification [J].Evolutionary Biology,2010,37:227-233.
[10] VINOGRADOV A E.Selfish DNA is maladaptive:evidence from the plant red list [J].Trends in Genetics,2003,19:609-614.
[11] GRIME J P,MOWFORTH M A.Variation in genome size:an ecological interpretation [J].Nature,1982,299:151-153.
[12] PRICE H J,CHAMBERS K L,BACHMANN K.Geographic and ecological distribution of genomic DNA content variation in Microseris douglasii (Asteraceae) [J].Botanical Gazette,1981,142(3):415-426.
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[15] RESKI R,FAUST M,WANG X H,et al.Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.[J].Molecular and General Genetics,1994,244:352-359.
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[17] RENZAGLIA K S,RASCH E M,PIKE L M.Estimates of nuclear DNA content in bryophyte sperm cells:phylogenetic considerations [J].American Journal of Botany,1995,82:18-25.
[18] VOGLMAYR H.Nuclear DNA amounts in Mosses (Musci) [J].Annals of Botany,2000,85:531-546.
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[25] DOLEZEL J,GOHDE W.Sex determination in dioecious plants Melandrium album and M.rubrum using high-resolution flow cytometry [J].Cytometry,1995,19:103-106.
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[27] 金亮,张宪银,薛庆中.分离缓冲液对水稻细胞核悬液DNA分辨率效应比较 [J].浙江农业学报,2007,19(2):93-96.JIN L,ZHANG X Y,XUE Q Z.Comparison on the effects of different isolation buffer on the DNA resolution of the cell nucleus suspensions from rice [J].Acta Agriculturae Zhejiangensis,2007,19(2):93-96.
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[29] BENNETT M D,SMITH J B.Nuclear DNA amounts in angiosperms [J].Philosophical Transactions of the Royal Society of London,1976,274(933):227-274.
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[32] EDUARDO A M,MOSCONE,MONIKA B,et al.Analysis of Nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and feulgen densitometry [J].Annals of Botany,2003,92:21-29.
[33] ZHAO X F,CHEN L H,LI Z Y,et al.Flow cytometry and its application in the microorganisms [J].Henan Agriculture Science,2005,6:5-9.
[34] DOLEZEL J.Flow cytometric analysis of nuclear DNA content in higher plants [J].Phytochemical Analysis,1991,2(4):143-154.
[35] BAINARD J D,FAZEKAS A J,NEWMASTER S G.Methodology significantly affects genome size estimates:quantitative evidence using bryophytes [J].Cytometry Part A,2010,77A (8):725-732.
[36] 方其,郭水良,印丽萍.应用流式细胞术测定植物DNAC-值的实验方法研究 [J].植物检疫,2011,25(2):40-44.FANG Q,GUO S L,YIN L P.On method of application of flow cytometry to determine plant DNA C-value [J].Plant Quarantine,2011,25(2):40-44.
[37] CHEN G Q,GUO S L,YIN L P.Applying DNA C-values to evaluate invasiveness of angiosperms:validity and limitation [J].Biological Invasions,2010,12(5):1335-1348.
[38] 崔明昆.苔藓植物的染色体多样性 [J].生物学杂志,2001,18(3):8-10.CUI M K.On diversity of bryophyte chromosome [J].Journal of Biology,2001,18(3):8-10.
[39] 郑敏.中国苔类植物染色体研究 [D].上海:华东师范大学,2007.ZHENG M.Cytological studies on liverworts from China [D].Shanghai:East China Normal University,2007.
[40] 陈圆圆,郭水良,曹同.藓类植物的无性繁殖及其应用 [J].生态学杂志,2008,27(6):993-998.CHEN Y Y,GUO S L,CAO T.Asexua l reproduction of moss and its applications [J].Chinese Journal of Ecology,2008,27(6):993-998.
[41] BAINARD J D,NEWMASTER S G.Endopolyploidy in bryophytes:widespread in mosses and absent in liverworts [J].Journal of Botany,2010:316356.
[2] FLAVELL R B.The molecular characterization and organization of plant chromosomal DNA sequences [J].Annual Review of Plant Physiology,1980,31:569-596.
[3] CAVALIER-SMITH T.Eukaryote gene numbers,non-coding DNA and genome size [M]//The Evolution of Genome Size.Chichester,Britain:John Wiley & Sons,1985:69-103.
[4] BENNETT M D,LEITCH L J.Nuclear DNA amounts in angiosperms:progress,problems and prospects [J].Annals of Botany,2005,95:45-90.
[5] 郭水良,周平,印丽萍,等.植物DNA C-值在种间和种内的变异及其生物学意义 [J].上海师范大学学报(自然科学版),2011,40(1):102-110.GUO S L,ZHOU P,YIN L P,et al.Interspecific and intraspecific variations of plant DNA C-values and its biological significance [J].Journal of Shanghai Normal University (Natural Sciences),2011,40(1):102-110.
[6] OHRI D,FRISCH R M,HANELT P.Evolution of genome size in Allium (Alliaceae) [J].Plant Systematics and Evolution,1998,210:57-86.
[7] BENNETT M D,BHANDOL P,LEITCH I J.Nuclear DNA amounts in angiosperms and their modern uses [J].Annals of Botany,2000,86:859-909.
[8] DOLE?ALOVA I,LEBEDA A,JANECEK J,et al.Variation in chromosome numbers and nuclear DNA contents in genetic resources of Lactuca L.species (Asteraceae) [J].Genetic Resources and Crop Evoluion,2002,49:383-395.
[9] KRAAIJEVELD K.Genome size and species diversification [J].Evolutionary Biology,2010,37:227-233.
[10] VINOGRADOV A E.Selfish DNA is maladaptive:evidence from the plant red list [J].Trends in Genetics,2003,19:609-614.
[11] GRIME J P,MOWFORTH M A.Variation in genome size:an ecological interpretation [J].Nature,1982,299:151-153.
[12] PRICE H J,CHAMBERS K L,BACHMANN K.Geographic and ecological distribution of genomic DNA content variation in Microseris douglasii (Asteraceae) [J].Botanical Gazette,1981,142(3):415-426.
[13] REEVES G,FRANCIS D,DAVIES S,et al.Genome Size is negatively correlated with altitude in natural populations of Dactylis glomerata [J].Annals of Botany,1998,82 (Suppl.A):99-105.
[14] BENNETT M D,LEITCH I J.Plant DNA C-values database [DB/OL].(2012-12-01)[2018-04-15].http://data.kew.org/cvalues/.
[15] RESKI R,FAUST M,WANG X H,et al.Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.[J].Molecular and General Genetics,1994,244:352-359.
[16] TEMSCH E M,GREILHUBER J,KRISAI R.Genome size in Sphagnum (peat moss) [J].Botanica Acta,1998,111:325-330.
[17] RENZAGLIA K S,RASCH E M,PIKE L M.Estimates of nuclear DNA content in bryophyte sperm cells:phylogenetic considerations [J].American Journal of Botany,1995,82:18-25.
[18] VOGLMAYR H.Nuclear DNA amounts in Mosses (Musci) [J].Annals of Botany,2000,85:531-546.
[19] GREILHUBER J,OBERMAYER R,LEITCH I J,et al.Bryophyte DNA C-values database [DB/OL].(2010-12-01) [2018-04-15].http://www.kew.org/cvalues/.
[20] DOLEZEL J,BINAROVA P,LUCRETTI S.Analysis of nuclear DNA content in plant cells by flow cytometry [J].Biologia Plantarum,1989,31:113-120.
[21] ARUMUGANATHAN K,EARLE E D.Estimation of nuclear DNA content of plants by flow cytometry [J].Plant Molecular Biology Reporter,1991,9:229-241.
[22] MARIE D,BROWN S C.A cytometric exercise in plant DNA histograms with 2C values for 70 species [J].Biology of the Cell,1993,78:41-51.
[23] OTTO F J.DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA [M]//DARZYNKIEWICKZ Z,CRISSMAN H A.Methods in Cell Biology.San Diego:Academic Press,1990,33:105-110.
[24] JOHNSTON J S,BENNETT M D,RAYBURH A L H J,et al.Reference standards for determination of DNA content of plant nuclei [J].American Journal of Botany,1999,86:609-613.
[25] DOLEZEL J,GOHDE W.Sex determination in dioecious plants Melandrium album and M.rubrum using high-resolution flow cytometry [J].Cytometry,1995,19:103-106.
[26] 杜立颖,冯仁青.流式细胞术 [M].北京:北京大学出版社,2008.DU L Y,FENG R Q.Flow Cytometry [M].Beijing:Press of Peking University,2008.
[27] 金亮,张宪银,薛庆中.分离缓冲液对水稻细胞核悬液DNA分辨率效应比较 [J].浙江农业学报,2007,19(2):93-96.JIN L,ZHANG X Y,XUE Q Z.Comparison on the effects of different isolation buffer on the DNA resolution of the cell nucleus suspensions from rice [J].Acta Agriculturae Zhejiangensis,2007,19(2):93-96.
[28] SEKER M,TUZCU O,OLLITRAULT P.Comparison of nuclear DNA content of citrus rootstock populations by flow cytometry analysis.[J].Plant Breeding,2003,122:169-172.
[29] BENNETT M D,SMITH J B.Nuclear DNA amounts in angiosperms [J].Philosophical Transactions of the Royal Society of London,1976,274(933):227-274.
[30] BRITTEN R J,GRAHAM D E,NEUFELD B R.Analysis of repeating DNA sequences by reassociation [M]//GROSSMAN L,MOLDAVE K.Methods in Enzymology.New York:Academic Press,1974,29:363-418.
[31] BENNETT M D,SMITH J B.Nuclear DNA amounts in angiosperms [J].Philosophical Transactions of the Royal Society of London,1991,334:309-345.
[32] EDUARDO A M,MOSCONE,MONIKA B,et al.Analysis of Nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and feulgen densitometry [J].Annals of Botany,2003,92:21-29.
[33] ZHAO X F,CHEN L H,LI Z Y,et al.Flow cytometry and its application in the microorganisms [J].Henan Agriculture Science,2005,6:5-9.
[34] DOLEZEL J.Flow cytometric analysis of nuclear DNA content in higher plants [J].Phytochemical Analysis,1991,2(4):143-154.
[35] BAINARD J D,FAZEKAS A J,NEWMASTER S G.Methodology significantly affects genome size estimates:quantitative evidence using bryophytes [J].Cytometry Part A,2010,77A (8):725-732.
[36] 方其,郭水良,印丽萍.应用流式细胞术测定植物DNAC-值的实验方法研究 [J].植物检疫,2011,25(2):40-44.FANG Q,GUO S L,YIN L P.On method of application of flow cytometry to determine plant DNA C-value [J].Plant Quarantine,2011,25(2):40-44.
[37] CHEN G Q,GUO S L,YIN L P.Applying DNA C-values to evaluate invasiveness of angiosperms:validity and limitation [J].Biological Invasions,2010,12(5):1335-1348.
[38] 崔明昆.苔藓植物的染色体多样性 [J].生物学杂志,2001,18(3):8-10.CUI M K.On diversity of bryophyte chromosome [J].Journal of Biology,2001,18(3):8-10.
[39] 郑敏.中国苔类植物染色体研究 [D].上海:华东师范大学,2007.ZHENG M.Cytological studies on liverworts from China [D].Shanghai:East China Normal University,2007.
[40] 陈圆圆,郭水良,曹同.藓类植物的无性繁殖及其应用 [J].生态学杂志,2008,27(6):993-998.CHEN Y Y,GUO S L,CAO T.Asexua l reproduction of moss and its applications [J].Chinese Journal of Ecology,2008,27(6):993-998.
[41] BAINARD J D,NEWMASTER S G.Endopolyploidy in bryophytes:widespread in mosses and absent in liverworts [J].Journal of Botany,2010:316356.