HPLC法同时测定加味四妙颗粒中6种成分
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摘要
目的建立HPLC法同时测定加味四妙颗粒(大血藤、黄柏、甘草等)中红景天苷、黄柏碱、甘草酸铵、延胡索乙素、盐酸小檗碱、甘草苷的含有量。方法该药物80%甲醇提取液的分析采用Agilent ZORBAX SB-C18色谱柱(250 mm×4. 6 mm,5μm);以乙腈-0. 1%磷酸为流动相,梯度洗脱;体积流量1. 0 m L/min;柱温30℃;检测波长220、280、250 nm。结果 6种成分在各自范围内线性关系良好(r> 0. 999 0),平均加样回收率98. 98%～100. 11%,RSD 0. 81%～1. 52%。结论该方法简便准确、专属性强,可用于加味四妙颗粒的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of salidroside,phellodendrine,ammonium glycyrrhizinate,tetrahydropalmatine,berberine hydrochloride and liquiritin in Supplemented Simiao Granules( Sargentodoxae Caulis,Phellodendri chinensis Cortex,Glycyrrhizae Radix et Rhizoma,etc.). METHODS The analysis of 80% methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent ZORBAX SB-C18column( 250 mm×4. 6 mm,5 μm),with the mobile phase comprising of acetonitrile-0. 1%phosphoric acid flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelengths were set at220,280,250 nm. RESULTS Six constituents showed good linear relationships within their own ranges( r >0. 999 0), whose average recoveries were 98. 98%-100. 11% with the RSDs of 0. 81%-1. 52%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Supplemented Simiao Granules.
AIM To establish an HPLC method for the simultaneous content determination of salidroside,phellodendrine,ammonium glycyrrhizinate,tetrahydropalmatine,berberine hydrochloride and liquiritin in Supplemented Simiao Granules( Sargentodoxae Caulis,Phellodendri chinensis Cortex,Glycyrrhizae Radix et Rhizoma,etc.). METHODS The analysis of 80% methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent ZORBAX SB-C18column( 250 mm×4. 6 mm,5 μm),with the mobile phase comprising of acetonitrile-0. 1%phosphoric acid flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelengths were set at220,280,250 nm. RESULTS Six constituents showed good linear relationships within their own ranges( r >0. 999 0), whose average recoveries were 98. 98%-100. 11% with the RSDs of 0. 81%-1. 52%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Supplemented Simiao Granules.
引文
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[2]谭雄斯. HPLC法同时测定五粒回春丸中8种成分[J].中成药,2018,40(1):110-113.
[3]钱珺,谢凡,石燕红,等. HPLC法同时测定白芍配方颗粒中5种成分[J].中成药,2018,40(1):117-120.
[4]马瑞丽,于小凤,徐秀泉,等.大血藤的化学成分及药理作用研究进展[J].中国野生植物资源,2012,31(6):1-5.
[5]王欢,毕福钧,林彤,等.延胡索HPLC指纹图谱研究及9种生物碱含量测定[J].中药材,2017,40(3):624-629.
[6]廉莲,万国盛,贾伟利,等. HPLC同时测定黄柏中6种化学成分含量[J].中国实验方剂学杂志,2013,19(2):94-97.
[7]张明发,沈雅琴.甘草及其活性成分对生殖系统药理作用研究进展[J].药物评价研究,2014,37(4):367-374.
[8]郭明晔,张燕玲,李洋,等. HPLC法同时测定甘草及甘草头中4种成分含量[J].世界科学技术(中医药现代化),2014,16(2):358-363.
[9]李浩,赵凤春,袁贤达,等.大血藤药材和饮片中酚类和皂苷类成分的含量[J].中国中药杂志,2015,40(10):1865-1871.
[10]潘超,张莉,王玉. HPLC法同时测定二妙丸中黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱的含量[J].中国生化药物杂志,2012,33(4):361-364.
[11]杨颜芳,张贵君,王晶娟,等.甘草干姜汤中8种药效组分的含量测定[J].天然产物研究与开发,2017,29(5):810-814.
[12]韩雪娇,郭娜,朱美宣,等.红景天苷药理作用及其作用机理研究进展[J].中国生化药物杂志,2015,35(1):171-175.
[13]李荣,蔡青青,牛彦兵,等.生、熟延胡索饮片药理作用的对比研究[J].中国实验方剂学杂志,2014,20(19):133-137.
[14]梁园园,于天浩,陈萍,等.黄柏的药理活性及在化妆品中的应用[J].辽宁中医药大学学报,2013,15(1):149-151.
[15]张友波,徐嵬,杨秀伟,等. RP-HPLC法同时测定不同产地甘草中9个主要成分的含量[J].药物分析杂志,2013,33(2):214-219,229.
[16]杨晓露,刘朵,卞卡,等.甘草总黄酮及其成分体外抗炎活性及机制研究[J].中国中药杂志,2013,38(1):99-104.
[17]严永旺,肖兰,周旭,等.绿原酸的药理作用及药用研发对策[J].中国药房,2017,28(19):2729-2732.