地龙蛋白多肽类成分的研究进展
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摘要
动物药地龙药用历史悠久,资源丰富,为临床常用中药之一。现代研究表明蛋白多肽类为地龙主要成分,具有抗凝血、抗脑卒中、抗菌、抗纤维化等多方面的药理作用,已被应用于心脑血管等多种疾病的治疗。对1983年至今发现的48种地龙蛋白多肽类成分的名称、相对分子质量、氨基酸序列、等电点及其活性等进行总结,同时对地龙蛋白多肽类成分的药理作用研究进展进行较全面地综述,以期为地龙蛋白多肽类成分的深入研究与开发利用提供参考。
Earthworm is one of animal drugs in Chinese materia medica. It was commonly used in clinic with rich resources in China. Modern studies showed that proteins and peptides were the main components in earthworm. It has been used for treating cardiovascular and cerebrovascular diseases because of its various activities, such as anticoagulant, anti-stroke, antibacterial, and antifibrotic activities, etc. In this review, 48 proteins and peptides from different species of earthworm reported since 1983 were summarized, including their names, molecular weights, amino acid sequences, isoelectric points, and activities. In addition, its pharmacological effects of earthworm proteins and peptides were summarized. In all, it will provide a scientific basis for the further study and comprehensive utilization of proteins and peptides of earthworm.
Earthworm is one of animal drugs in Chinese materia medica. It was commonly used in clinic with rich resources in China. Modern studies showed that proteins and peptides were the main components in earthworm. It has been used for treating cardiovascular and cerebrovascular diseases because of its various activities, such as anticoagulant, anti-stroke, antibacterial, and antifibrotic activities, etc. In this review, 48 proteins and peptides from different species of earthworm reported since 1983 were summarized, including their names, molecular weights, amino acid sequences, isoelectric points, and activities. In addition, its pharmacological effects of earthworm proteins and peptides were summarized. In all, it will provide a scientific basis for the further study and comprehensive utilization of proteins and peptides of earthworm.
引文
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[5]杜航,孙佳明,郭晓庆,等.地龙的化学成分及药理作用[J].吉林中医药,2014,34(7):707-709.
[6]Verma M K,Pulicherla K K.Enzyme promiscuity in earthworm serine protease:Substrate versatility and therapeutic potential[J].Amino Acids,2016,48(4):941-948.
[7]袁渊,沈宏萍.地龙活性蛋白药理学作用研究进展[J].中国现代医药杂志,2014,16(3):107-109.
[8]杨新,刘欣,万明,等.地龙抗凝血活性物质研究进展[J].江汉大学学报:自然科学版,2017,45(1):83-88.
[9]Mihara H,Sumi H,Akazawa T,et al.Fibinolytic enzyme extracted from the earthworm[J].Thromb Haemostas,1983,50(1):258-263.
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[13]Yang J S,Ru B G.Purification and characterization of an SDS-activated fibrinolytic enzyme from Eisenia fetida[J].Biochem Mol Biol,1997,118(3):623-631.
[14]Lassegues M,Milochau A,Doignon F,et al.Sequence and expression of an Eisenia-fetida-derived cDNA clone that encodes the 40-kDa fetidin antibacterial protein[J].Eur J Biochem,1997,246(3):756-762.
[15]Cho I H,Choi E S,Lim H G,et al.Purification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus[J].J Biochem Mol Biol,2004,37(2):199-205.
[16]Verma M K,Pulicherla K K.Broad substrate affinity and catalytic diversity of fibrinolytic enzyme from Pheretima posthumous-Purification and molecular characterization study[J].Int J Biol Macromol,2017,95:1011-1021.
[17]程牛亮,牛勃,张祖珣,等.双胸蚓纤溶酶的纯化及性质[J].中国生物化学与分子生物学报,1990,6(2):186-190.
[18]郑国平,程牛亮.双胸蚓纤溶酶III的分离纯化及其性质研究[J].山西医科大学学报,1996,27(2):81-83.
[19]于保锋,刘志贞,张悦红,等.赤子爱胜蚓核酸酶样蛋白的纯化及其活性[J].中国生物制品学杂志,2013,26(5):705-709.
[20]于保锋,刘志贞,张悦红,等.赤子爱胜蚓核酸酶样蛋白的一级结构特征[J].中国组织工程研究,2014,18(15):2390-2396.
[21]杨四成,刘晓英,李令媛,等.蚯蚓纤溶酶的分离纯化及部分序列的测定[J].中国生物化学与分子生物学报,1997,13(3):292-296.
[22]谢江碧,郭振泉,翁宁,等.一种凋亡相关蚯蚓丝氨酸蛋白酶的纯化、活性鉴定及部分性质研究[J].生物化学与生物物理进展,2003,30(3):453-460.
[23]Feng W,Chao W,Mei L,et al.Purification,characterization and crystallization of a group of earthworm fibrinolytic enzymes from Eisenia fetida[J].Biotechnol Lett,2003,25(13):1105-1109.
[24]Tang Y,Zhang J,Gui L,et al.Crystallization and preliminary X-ray analysis of earthworm fibrinolytic enzyme component A from Eisenia fetida[J].Acta Crystallogr Sect C-Struct Chem,2000,56(12):1659-1661.
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